LOCUS BC000751 1290 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens eukaryotic translation initiation factor 5A, mRNA
(cDNA clone MGC:2453 IMAGE:2961296), complete cds.
ACCESSION BC000751
VERSION BC000751.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1290)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1290)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000751.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 2 Row: j Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34147551.
FEATURES Location/Qualifiers
source 1..1290
/db_xref="H-InvDB:HIT000029893"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:2453 IMAGE:2961296"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1290
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/gene_synonym="uORF"
/gene_synonym="uORF A"
/db_xref="GeneID:1984"
/db_xref="HGNC:HGNC:3300"
/db_xref="MIM:600187"
CDS 125..589
/gene="EIF5A"
/gene_synonym="EIF-5A"
/gene_synonym="EIF5A1"
/gene_synonym="uORF"
/gene_synonym="uORF A"
/codon_start=1
/product="eukaryotic translation initiation factor 5A"
/protein_id="AAH00751.1"
/db_xref="GeneID:1984"
/db_xref="HGNC:HGNC:3300"
/db_xref="MIM:600187"
/translation="MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMS
TSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLL
QDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK"
BASE COUNT 306 a 343 c 354 g 287 t
ORIGIN
1 gcggcggcgg cggtagaggc ggcggcggcg gcggcagcgg gctcggaggc agcggttggg
61 ctcgcggcga gcggacgggg tcgagtcagt gcgttcgcgc gagttggaat cgaagcctct
121 taaaatggca gatgacttgg acttcgagac aggagatgca ggggcctcag ccaccttccc
181 aatgcagtgc tcagcattac gtaagaatgg ctttgtggtg ctcaaaggcc ggccatgtaa
241 gatcgtcgag atgtctactt cgaagactgg caagcacggc cacgccaagg tccatctggt
301 tggtattgac atctttactg ggaagaaata tgaagatatc tgcccgtcaa ctcataatat
361 ggatgtcccc aacatcaaaa ggaatgactt ccagctgatt ggcatccagg atgggtacct
421 atcactgctc caggacagcg gggaggtacg agaggacctt cgtctccctg agggagacct
481 tggcaaggag attgagcaga agtacgactg tggagaagag atcctgatca cggtgctgtc
541 tgccatgaca gaggaggcag ctgttgcaat caaggccatg gcaaaataac tggctcccag
601 gatggcggtg gtggcagcag tgatcctctg aacctgcaga ggccccctcc ccgagcctgg
661 cctggctctg gcccggtcct aagctggact cctcctacac aatttatttg acgttttatt
721 ttggttttcc ccaccccctc aatctgtcgg ggagcccctg cccttcacct agctcccttg
781 gccaggagcg agcgaagctg tggccttggt gaagctgccc tcctcttctc ccctcacact
841 acagccctgg tgggggagaa gggggtgggt gctgcttgtg gtttagtctt tttttttttt
901 tttttttttt ttttaaattc aatctggaat cagaaagcgg tggattctgg caaatggtcc
961 ttgtgccctc cccactcatc cctggtctgg tcccctgttg cccatagccc tttaccctga
1021 gcaccacccc aacagactgg ggaccagccc cctcgcctgc ctgtgtctct ccccaaaccc
1081 ctttagatgg ggagggaaga ggaggagagg ggaggggacc tgccccctcc tcaggcatct
1141 gggagggccc tgcccccatg ggctttaccc ttccctgcgg gctctctccc cgacacattt
1201 gttaaaatca aacctgaata aaactacaag tttaatatga aaaaaaaaaa aaaaaaaaaa
1261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//