LOCUS BC000718 1759 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens dynactin 2 (p50), mRNA (cDNA clone MGC:1336
IMAGE:3504109), complete cds.
ACCESSION BC000718
VERSION BC000718.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1759)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1759)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000718.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 7 Row: a Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13259506.
FEATURES Location/Qualifiers
source 1..1759
/db_xref="H-InvDB:HIT000029863"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1336 IMAGE:3504109"
/tissue_type="Placenta, choriocarcinoma"
/clone_lib="NIH_MGC_21"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1759
/gene="DCTN2"
/gene_synonym="DCTN50"
/gene_synonym="DYNAMITIN"
/gene_synonym="RBP50"
/db_xref="GeneID:10540"
/db_xref="MIM:607376"
CDS 133..1338
/gene="DCTN2"
/gene_synonym="DCTN50"
/gene_synonym="DYNAMITIN"
/gene_synonym="RBP50"
/codon_start=1
/product="DCTN2 protein"
/protein_id="AAH00718.1"
/db_xref="GeneID:10540"
/db_xref="MIM:607376"
/translation="MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVE
HIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQK
YQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGP
DAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKF
SQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVL
DQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLV
TIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASI
DERMKKLGK"
BASE COUNT 489 a 459 c 457 g 354 t
ORIGIN
1 gagacttcct gctcatctgc cgctcccttt gccgccgcct tagcccggga cccgaaccca
61 gcctctcccc tacccgaaca ccggccccgg ctccaccgag gcccgggtcc cccagcccgt
121 ctcgccgccg ccatggcgga ccctaaatac gccgaccttc ccggcattgc caggaatgag
181 ccagatgttt atgaaactag cgacctacct gaggatgatc aagcggagtt cgatgcggag
241 gagctgacaa gcacaagtgt ggaacacatc attgtcaatc ctaatgctgc ctatgacaag
301 ttcaaggaca agagagtggg gacaaaggga cttgatttct cagatcgtat tggaaaaacc
361 aagaggacag gatatgaatc tggagaatat gagatgcttg gagagggtct gggagtgaag
421 gagacacccc agcaaaagta ccagcgccta ctgcatgagg tccaagagct gacaactgaa
481 gttgaaaaaa tcaagacgac agtgaaggag tcagccacag aggagaagct gacccctgtg
541 ttgctggcta aacagctggc agccctgaag cagcagctgg ttgcttccca cctggagaag
601 ctgctgggac cagatgctgc aatcaacctt accgaccccg atggcgccct ggctaagcgc
661 ctactactgc agctggaagc aacaaagaac agcaaagggg gatcaggggg aaaaaccact
721 gggacccccc cagatagcag ccttgtcact tatgaactac attctcggcc tgagcaggac
781 aagttctctc aagctgccaa agtcgcagaa cttgaaaagc gcctgacaga gctggagaca
841 gctgtacgtt gtgatcagga tgctcagaat cccctttctg caggtctaca gggagcctgt
901 ctcatggaga ctgtagagct gttgcaagca aaggtgagcg ccctagacct tgcagttttg
961 gatcaagtgg aggctcggct acagagtgtc ctgggaaagg tgaacgagat tgccaagcat
1021 aaagcctctg tagaagatgc agatacacaa agcaaggtgc accagctata tgaaactata
1081 cagcgctgga gccccattgc ctccaccctc cctgagctgg tgcagagact tgtcaccatc
1141 aagcagctgc acgagcaagc catgcagttt ggtcagctcc tgacacactt ggataccacc
1201 cagcagatga ttgctaattc cttgaaggac aataccaccc tcttgaccca ggtgcagaca
1261 accatgcgtg aaaacctggc cacagttgag gggaactttg ccagcattga tgaacggatg
1321 aagaagctgg gaaagtgagc acatttggga gctggagaac aggggttatc cctacccctg
1381 tgaactctgt taacagctta catagggttt cccctttact ataactctag catccccatc
1441 ccatttgaca ctgggggcaa gggttcttct tgcatgtggg gtttataccc ctcccctgat
1501 gaatacagag tggtagctag gggttggtta tcatcagaag gtggtctccc ctcaggcctg
1561 ggggataagg acgtgggccc agccacatgc caactcatgt ccaatactgc tttgcctggt
1621 gtggggaagg attgggtctt gtcccccaac acagcttctg tggctgactg taatactgta
1681 caactgtttc tgaccattaa atgctgttgt actctgaaaa aaaaaaaaaa aaaaaaaaaa
1741 aaaaaaaaaa aaaaaaaaa
//