LOCUS BC000479 2729 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens v-akt murine thymoma viral oncogene homolog 1, mRNA
(cDNA clone MGC:8686 IMAGE:2964603), complete cds.
ACCESSION BC000479
VERSION BC000479.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2729)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2729)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000479.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: p Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 62241012.
FEATURES Location/Qualifiers
source 1..2729
/db_xref="H-InvDB:HIT000029663"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8686 IMAGE:2964603"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2729
/gene="AKT1"
/gene_synonym="PKB"
/gene_synonym="PRKBA"
/gene_synonym="RAC"
/gene_synonym="RAC-ALPHA"
/db_xref="GeneID:207"
/db_xref="HGNC:HGNC:391"
/db_xref="MIM:164730"
CDS 276..1718
/gene="AKT1"
/gene_synonym="PKB"
/gene_synonym="PRKBA"
/gene_synonym="RAC"
/gene_synonym="RAC-ALPHA"
/codon_start=1
/product="v-akt murine thymoma viral oncogene homolog 1"
/protein_id="AAH00479.1"
/db_xref="GeneID:207"
/db_xref="HGNC:HGNC:391"
/db_xref="MIM:164730"
/translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQD
VDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTA
IQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGT
FGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQ
THDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLK
LENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWG
LGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRL
GGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITIT
PPDQDDSMECVDSERRPHFPQFSYSASGTA"
BASE COUNT 552 a 815 c 826 g 536 t
ORIGIN
1 ggcggccgag caccgagcgc tgggcaccgg gcaccgagcg gcggcggcac gcgaggcccg
61 gccccgagca gcgcccccgc ccgccgcggc ctccagcccg gccccgccca gcgccggccc
121 gcggggatgc ggagcggcgg gcgccggagg ccgcggcccg gctaggcccg cgctcgcgcc
181 cggacgcggc ggcccgaggc tgtggccagg ccagctgggc tcggggagcg ccagcctgag
241 aggagcgcgt gagcgtcgcg ggagcctcgg gcaccatgag cgacgtggct attgtgaagg
301 agggttggct gcacaaacga ggggagtaca tcaagacctg gcggccacgc tacttcctcc
361 tcaagaatga tggcaccttc attggctaca aggagcggcc gcaggatgtg gaccaacgtg
421 aggctcccct caacaacttc tctgtggcgc agtgccagct gatgaagacg gagcggcccc
481 ggcccaacac cttcatcatc cgctgcctgc agtggaccac tgtcatcgaa cgcaccttcc
541 atgtggagac tcctgaggag cgggaggagt ggacaaccgc catccagact gtggctgacg
601 gcctcaagaa gcaggaggag gaggagatgg acttccggtc gggctcaccc agtgacaact
661 caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgcgtg accatgaacg
721 agtttgagta cctgaagctg ctgggcaagg gcactttcgg caaggtgatc ctggtgaagg
781 agaaggccac aggccgctac tacgccatga agatcctcaa gaaggaagtc atcgtggcca
841 aggacgaggt ggcccacaca ctcaccgaga accgcgtcct gcagaactcc aggcacccct
901 tcctcacagc cctgaagtac tctttccaga cccacgaccg cctctgcttt gtcatggagt
961 acgccaacgg gggcgagctg ttcttccacc tgtcccggga gcgtgtgttc tccgaggacc
1021 gggcccgctt ctatggcgct gagattgtgt cagccctgga ctacctgcac tcggagaaga
1081 acgtggtgta ccgggacctc aagctggaga acctcatgct ggacaaggac gggcacatta
1141 agatcacaga cttcgggctg tgcaaggagg ggatcaagga cggtgccacc atgaagacct
1201 tttgcggcac acctgagtac ctggcccccg aggtgctgga ggacaatgac tacggccgtg
1261 cagtggactg gtgggggctg ggcgtggtca tgtacgagat gatgtgcggt cgcctgccct
1321 tctacaacca ggaccatgag aagctttttg agctcatcct catggaggag atccgcttcc
1381 cgcgcacgct tggtcccgag gccaagtcct tgctttcagg gctgctcaag aaggacccca
1441 agcagaggct tggcgggggc tccgaggacg ccaaggagat catgcagcat cgcttctttg
1501 ccggtatcgt gtggcagcac gtgtacgaga agaagctcag cccacccttc aagccccagg
1561 tcacgtcgga gactgacacc aggtattttg atgaggagtt cacggcccag atgatcacca
1621 tcacaccacc tgaccaagat gacagcatgg agtgtgtgga cagcgagcgc aggccccact
1681 tcccccagtt ctcctactcg gccagcggca cggcctgagg cggcggtgga ctgcgctgga
1741 cgatagcttg gagggatgga gaggcggcct cgtgccatga tctgtattta atggttttta
1801 tttctcgggt gcatttgaga gaagccacgc tgtcctctcg agcccagatg gaaagacgtt
1861 tttgtgctgt gggcagcacc ctcccccgca gcggggtagg gaagaaaact atcctgcggg
1921 ttttaattta tttcatccag tttgttctcc gggtgtggcc tcagccctca gaacaatccg
1981 attcacgtag ggaaatgtta aggacttctg cagctatgcg caatgtggca ttggggggcc
2041 gggcaggtcc tgcccatgtg tcccctcact ctgtcagcca gccgccctgg gctgtctgtc
2101 accagctatc tgtcatctct ctggggccct gggcctcagt tcaacctggt ggcaccagat
2161 gcaacctcac tatggtatgc tggccagcac cctctcctgg gggtggcagg cacacagcag
2221 ccccccagca ctaaggccgt gtctctgagg acgtcatcgg aggctgggcc cctgggatgg
2281 gaccagggat gggggatggg ccagggttta cccagtggga cagaggagca aggtttaaat
2341 ttgttattgt gtattatgtt gttcaaatgc attttggggg tttttaatct ttgtgacagg
2401 aaagccctcc cccttcccct tctgtgtcac agttcttggt gactgtccca ccgggagcct
2461 ccccctcaga tgatctctcc acggtagcac ttgacctttt cgacgcttaa cctttccgct
2521 gtcgccccag gccctccctg actccctgtg ggggtggcca tccctgggcc cctccacgcc
2581 tcctggccag acgctgccgc tgccgctgca ccacggcgtt tttttacaac attcaacttt
2641 agtattttta ctattataat ataatatgga accttccctc caaattcttc aataaaagtt
2701 gcttttcaaa aaaaaaaaaa aaaaaaaaa
//