LOCUS BC000447 576 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens macrophage migration inhibitory factor
(glycosylation-inhibiting factor), mRNA (cDNA clone MGC:8444
IMAGE:2821346), complete cds.
ACCESSION BC000447
VERSION BC000447.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 576)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 576)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000447.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: f Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505184.
FEATURES Location/Qualifiers
source 1..576
/db_xref="H-InvDB:HIT000029632"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8444 IMAGE:2821346"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..576
/gene="MIF"
/gene_synonym="GIF"
/gene_synonym="MMIF"
/db_xref="GeneID:4282"
/db_xref="HGNC:HGNC:7097"
/db_xref="MIM:153620"
CDS 82..429
/gene="MIF"
/gene_synonym="GIF"
/gene_synonym="MMIF"
/codon_start=1
/product="macrophage migration inhibitory factor
(glycosylation-inhibiting factor)"
/protein_id="AAH00447.1"
/db_xref="GeneID:4282"
/db_xref="HGNC:HGNC:7097"
/db_xref="MIM:153620"
/translation="MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVP
DQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDM
NAANVGWNNSTFA"
BASE COUNT 126 a 199 c 161 g 90 t
ORIGIN
1 agaagtcagg cacgtagctc agcggcggcc gcggcgcgtg cgtctgtgcc tctgcgcggg
61 tctcctggtc cttctgccat catgccgatg ttcatcgtaa acaccaacgt gccccgcgcc
121 tccgtgccgg acgggttcct ctccgagctc acccagcagc tggcgcaggc caccggcaag
181 cccccccagt acatcgcggt gcacgtggtc ccggaccagc tcatggcctt cggcggctcc
241 agcgagccgt gcgcgctctg cagcctgcac agcatcggca agatcggcgg cgcgcagaac
301 cgctcctaca gcaagctgct gtgcggcctg ctggccgagc gcctgcgcat cagcccggac
361 agggtctaca tcaactatta cgacatgaac gcggccaatg tgggctggaa caactccacc
421 ttcgcctaag agccgcaggg acccacgctg tctgcgctgg ctccacccgg gaacccgccg
481 cacgctgtgt tctaggcccg cccaccccaa ccttctggtg gggagaaata aacggtttag
541 agactaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//