LOCUS BC000419 1217 bp mRNA linear HUM 09-MAR-2007
DEFINITION Homo sapiens catechol-O-methyltransferase, mRNA (cDNA clone
MGC:8663 IMAGE:2964400), complete cds.
ACCESSION BC000419
VERSION BC000419.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1217)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1217)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000419.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: o Column: 6
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 6466449.
FEATURES Location/Qualifiers
source 1..1217
/db_xref="H-InvDB:HIT000029605"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8663 IMAGE:2964400"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1217
/gene="COMT"
/db_xref="GeneID:1312"
/db_xref="HGNC:HGNC:2228"
/db_xref="MIM:116790"
CDS 445..993
/gene="COMT"
/codon_start=1
/product="COMT protein"
/protein_id="AAH00419.2"
/db_xref="GeneID:1312"
/db_xref="HGNC:HGNC:2228"
/db_xref="MIM:116790"
/translation="MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPG
ARLITIEINPDCAAITQRMVDFAGMKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLD
HWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLE
YREVVDGLEKAIYKGPGSEAGP"
BASE COUNT 243 a 366 c 378 g 230 t
ORIGIN
1 ggcttctggg gcagctaggg ctgcccgccg cgctgcctgc gccggaccgg ggcgggtcca
61 gtcccgggcg ggccgtcgcg ggagagaaat aacatctgct ttgctgccga gctcagagga
121 gaccccagac ccctcccgca gccagagggc tggagcctgc tcagaggtgc tttgaagatg
181 ccggaggccc cgcctctgct gttggcagct gtgttgctgg gcctggtgct gctggtggtg
241 ctgctgctgc ttctgaggca ctggggctgg ggcctgtgcc ttatcggctg gaacgagttc
301 atcctgcagc ccatccacaa cctgctcatg ggtgacacca aggagcagcg catcctgaac
361 catgtgctgc agcatgcgga gcccgggaac gcacagagcg tgctggaggc cattgacacc
421 tactgcgagt agaaggagtg ggccatgaac gtgggcgaca agaaaggcaa gatcgtggac
481 gccgtgattc aggagcacca gccctccgtg ctgctggagc tgggggccta ctgtggctac
541 tcagctgtgc gcatggcccg cctgctgtca ccaggggcga ggctcatcac catcgagatc
601 aaccccgact gtgccgccat cacccagcgg atggtggatt tcgctggcat gaaggacaag
661 gtcacccttg tggttggagc gtcccaggac atcatccccc agctgaagaa gaagtatgat
721 gtggacacac tggacatggt cttcctcgac cactggaagg accggtacct gccggacacg
781 cttctcttgg aggaatgtgg cctgctgcgg aaggggacag tgctactggc tgacaacgtg
841 atctgcccag gtgcgccaga cttcctagca cacgtgcgcg ggagcagctg ctttgagtgc
901 acacactacc aatcgttcct ggaatacagg gaggtggtgg acggcctgga gaaggccatc
961 tacaagggcc caggcagcga agcagggccc tgactgcccc ccccggcccc cctctcgggc
1021 tctctcaccc agcctggtac tgaaggtgcc agacgtgctc ctgctgacct tctgcggctc
1081 cgggctgtgt cctaaatgca aagcacacct cggccgaggc ctgcgccctg acatgctaac
1141 ctctctgaac tgcaacactg gattgttctt ttttaagact caatcatgac ttctttacta
1201 aaaaaaaaaa aaaaaaa
//