LOCUS BC000396 1250 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2N (UBC13 homolog,
yeast), mRNA (cDNA clone MGC:8489 IMAGE:2822013), complete cds.
ACCESSION BC000396
VERSION BC000396.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1250)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1250)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000396.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: g Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 61175264.
FEATURES Location/Qualifiers
source 1..1250
/db_xref="H-InvDB:HIT000029585"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8489 IMAGE:2822013"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1250
/gene="UBE2N"
/gene_synonym="MGC8489"
/gene_synonym="UBC13"
/gene_synonym="UbcH-ben"
/db_xref="GeneID:7334"
/db_xref="HGNC:HGNC:12492"
/db_xref="MIM:603679"
CDS 93..551
/gene="UBE2N"
/gene_synonym="MGC8489"
/gene_synonym="UBC13"
/gene_synonym="UbcH-ben"
/codon_start=1
/product="ubiquitin-conjugating enzyme E2N (UBC13 homolog,
yeast)"
/protein_id="AAH00396.1"
/db_xref="GeneID:7334"
/db_xref="HGNC:HGNC:12492"
/db_xref="MIM:603679"
/translation="MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQD
SPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIR
TVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI"
BASE COUNT 357 a 262 c 269 g 362 t
ORIGIN
1 gtcccccgga agtggagccc gggacttcca ctcgtgcgtg aggcgagagg agccggagac
61 gagaccagag gccgaactcg ggttctgaca agatggccgg gctgccccgc aggatcatca
121 aggaaaccca gcgtttgctg gcagaaccag ttcctggcat caaagccgaa ccagatgaga
181 gcaacgcccg ttattttcat gtggtcattg ctggccctca ggattccccc tttgagggag
241 ggacttttaa acttgaacta ttccttccag aagaataccc aatggcagcc cctaaagtac
301 gtttcatgac caaaatttat catcctaatg tagacaagtt gggaagaata tgtttagata
361 ttttgaaaga taagtggtcc ccagcactgc agatccgcac agttctgcta tcgatccagg
421 ccttgttaag tgctcccaat ccagatgatc cattagcaaa tgatgtagcg gagcagtgga
481 agaccaacga agcccaagcc atagaaacag ctagagcatg gactaggcta tatgccatga
541 ataatattta aattgatacg atcatcaagt gtgcatcact tctcctgttc tgccaagact
601 tcctcctctt tgtttgcatt taatggacac agtcttagaa acattacaga ataaaaaagc
661 ccagacatct tcagtccttt ggtgattaaa tgcacattag caaatctatg tcttgtcctg
721 attcactgtc ataaagcatg agcagaggct agaagtatca tctggattgt tgtgaaacgt
781 ttaaaagcag tggcccctcc ctgcttttat tcatttcccc catcctggtt taagtataaa
841 gcactgtgaa tgaaggtagt tgtcaggtta gctgcagggg tgtgggtgtt tttattttat
901 tttattttat tttatttttg aggggggagg tagtttaatt ttatgggctc ctttccccct
961 tttttggtga tctaattgca ttggttaaaa gcagctaacc aggtctttag aatatgctct
1021 agccaagtct aactttattt agacgctgta gatggacaag cttgattgtt ggaaccaaaa
1081 tgggaacatt aaacaaacat cacagccctc actaataaca ttgctgtcaa gtgtagattc
1141 cccccttcaa aaaaagcttg tgaccatttt gtatggcttg tctggaaact tctgtaaatc
1201 ttatgtttta gtaaaatatt ttttgttatt ctaaaaaaaa aaaaaaaaaa
//