LOCUS BC000351 1747 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphate cytidylyltransferase 2, ethanolamine, mRNA
(cDNA clone MGC:8624 IMAGE:2961418), complete cds.
ACCESSION BC000351
VERSION BC000351.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1747)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1747)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000351.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: m Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4505650.
FEATURES Location/Qualifiers
source 1..1747
/db_xref="H-InvDB:HIT000029543"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8624 IMAGE:2961418"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1747
/gene="PCYT2"
/gene_synonym="ET"
/db_xref="GeneID:5833"
/db_xref="HGNC:HGNC:8756"
/db_xref="MIM:602679"
CDS 31..1200
/gene="PCYT2"
/gene_synonym="ET"
/codon_start=1
/product="phosphate cytidylyltransferase 2, ethanolamine"
/protein_id="AAH00351.1"
/db_xref="GeneID:5833"
/db_xref="HGNC:HGNC:8756"
/db_xref="MIM:602679"
/translation="MIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQ
ARAMGDYLIVGVHTDEEIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETL
DKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAH
HSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQPGETVIYV
AGAFDLFHIGHVDFLEKVHRLAERPYIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVL
ACRYVSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQI
DSGSNLTTDLIVQRIITNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF"
BASE COUNT 402 a 517 c 532 g 296 t
ORIGIN
1 ccaggctgtc cgcgccgccg ctgcggggcc atgatccgga acgggcgcgg ggctgcaggc
61 ggcgcagagc agccgggccc ggggggcagg cgcgccgtga gggtgtggtg cgatggctgc
121 tatgacatgg tgcattacgg ccactccaac cagctgcgcc aggcacgggc catgggtgac
181 tacctcatcg taggcgtgca caccgatgag gagatcgcca agcacaaggg gcccccggtg
241 ttcactcagg aggagagata caagatggtg caggccatca aatgggtgga cgaggtggtg
301 ccagcggctc cctacgtcac tacactagag accctggaca aatacaactg tgacttctgt
361 gttcacggca atgacatcac cctgactgta gatggccggg acacctatga ggaagtaaag
421 caggctggga ggtacagaga atgcaagcgc acgcaagggg tgtccaccac agacctcgtg
481 ggccgcatgc tgctggtaac caaagcccat cacagcagcc aggagatgtc ctctgagtac
541 cgggagtatg cagacagttt tggcaagtgc cctggtgggc ggaacccctg gaccggggta
601 tcccagttcc tgcagacatc tcagaagatc atccagtttg cttctgggaa ggagccccag
661 ccaggggaga cagtcatcta tgtggctggt gccttcgacc tgttccacat cgggcatgtg
721 gacttcctgg agaaggtgca caggctggca gagaggccct acatcatcgc gggcttacac
781 tttgaccagg aggtcaatca ctacaagggg aagaactacc ccatcatgaa tctgcatgaa
841 cggactctga gcgtgctggc ctgccggtac gtgtcagaag tggtgattgg agccccgtac
901 gcggtcacag cagagctcct aagtcacttc aaggtggacc tggtgtgtca cggcaagaca
961 gaaattatcc ctgacaggga tggctccgac ccataccagg agcccaagag aaggggcatc
1021 ttccgtcaga ttgacagtgg cagcaacctc accacagacc tcatcgtcca gcggatcatc
1081 accaacaggt tggagtatga ggcgcgaaac cagaagaagg aagccaagga gctggccttc
1141 ctggaggctg ccaggcagca ggcggcacag cccctggggg agcgcgatgg tgacttctaa
1201 cctggcagag gccctggccg gccctccccc tgctctgctt ctgcgccttc tgcgtttgga
1261 cataggactc tgcagggccg ccctctctaa ctggcctggc tctggaaggg ctggtgagga
1321 ctctgcctcc ttgcctgcct acaaggtgcc tggtttgcag caggctctcc gctctttcca
1381 gcaaagctgc tcagagaggg tgtccagcac agtggagagg ccggaagtga gacgggcaga
1441 cggcacctgc agcctgaaac gcaccgctcc tgcgtgcgcc cccacctggt ccccggatgc
1501 ccccaccacc tggacagagg ccacactgac tgcccaccca gctgtggcgg gaggtgcaga
1561 gcagagggct ttagggagca gtgactgcgg tcaccccttt agttctctgg gtgtagacca
1621 caccacctcc cactgggcac cccccaacac ggtgtcctgc cacccagcgc ctggctccag
1681 gaaaacacgc ttgccttcct tcccggcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
1741 aaaaaaa
//