LOCUS BC000347 2390 bp mRNA linear HUM 06-NOV-2003
DEFINITION Homo sapiens lectin, mannose-binding 2-like, mRNA (cDNA clone
IMAGE:2960965), partial cds.
ACCESSION BC000347
VERSION BC000347.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2390)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2390)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000347.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: k Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4557466.
FEATURES Location/Qualifiers
source 1..2390
/db_xref="H-InvDB:HIT000085831"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2960965"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2390
/gene="LMAN2L"
/gene_synonym="DKFZp564L2423"
/gene_synonym="MGC11139"
/gene_synonym="VIPL"
/db_xref="GeneID:81562"
CDS <1..1046
/gene="LMAN2L"
/gene_synonym="DKFZp564L2423"
/gene_synonym="MGC11139"
/gene_synonym="VIPL"
/codon_start=3
/product="LMAN2L protein"
/protein_id="AAH00347.2"
/db_xref="GeneID:81562"
/translation="AATLGPLGSWQQWRRCLSARDGSRMLLLLLLLGSGQGPQQVGAG
QTFEYLKREHSLSKPYQGVGTGSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVP
CFLRDWELQVHFKIHGQGKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTY
PNEEKQQERVFPYISAMVNNGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVK
RHLTIMMDIDGKHEWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVE
RTPEEEKLHRDVFLPSVDNMKLPEMTAPLPPLSGLALFLIVFFSLVFSVFAIVIGIIL
YNKWQEQSRKRFY"
misc_feature 144..827
/gene="LMAN2L"
/gene_synonym="DKFZp564L2423"
/gene_synonym="MGC11139"
/gene_synonym="VIPL"
/note="Lectin_leg-like; Region: Legume-like lectin family.
Lectins are structurally diverse proteins that bind to
specific carbohydrates. This family includes the VIP36 and
ERGIC-53 lectins. These two proteins were the first
recognised members of a family of animal lectins similar
(19-24%) to the leguminous plant lectins. The alignment
for this family aligns residues lying towards the
N-terminus, where the similarity of VIP36 and ERGIC-53 is
greatest. However, while Fiedler and Simons identified
these proteins as a new family of animal lectins, our
alignment also includes yeast sequences. ERGIC-53 is a
53kD protein, localised to the intermediate region between
the endoplasmic reticulum and the Golgi apparatus
(ER-Golgi-Intermediate Compartment, ERGIC). It was
identified as a calcium-dependent, mannose-specific
lectin. Its dysfunction has been associated with combined
factors V and VIII deficiency OMIM:227300 OMIM:601567,
suggesting an important and substrate-specific role for
ERGIC-53 in the glycoprotein- secreting pathway"
/db_xref="CDD:pfam03388"
BASE COUNT 552 a 580 c 626 g 632 t
ORIGIN
1 tggcggcgac tctgggaccc cttgggtcgt ggcagcagtg gcggcgatgt ttgtcggctc
61 gggatgggtc caggatgtta ctccttcttc ttttgttggg gtctgggcag gggccacagc
121 aagtcggggc gggtcaaacg ttcgagtact tgaaacggga gcactcgctg tcgaagccct
181 accagggtgt gggcacaggc agttcctcac tgtggaatct gatgggcaat gccatggtga
241 tgacccagta tatccgcctt accccagata tgcaaagtaa acagggtgcc ttgtggaacc
301 gggtgccatg tttcctgaga gactgggagt tgcaggtgca cttcaaaatc catggacaag
361 gaaagaagaa tctgcatggg gatggcttgg caatctggta cacaaaggat cggatgcagc
421 cagggcctgt gtttggaaac atggacaaat ttgtggggct gggagtattt gtagacacct
481 accccaatga ggagaagcag caagagcggg tattccccta catctcagcc atggtgaaca
541 acggctccct cagctatgat catgagcggg atgggcggcc tacagagctg ggaggctgca
601 cagccattgt ccgcaatctt cattacgaca ccttcctggt gattcgctac gtcaagaggc
661 atttgacgat aatgatggat attgatggca agcatgagtg gagggactgc attgaagtgc
721 ccggagtccg cctgccccgc ggctactact tcggcacctc ctccatcact ggggatctct
781 cagataatca tgatgtcatt tccttgaagt tgtttgaact gacagtggag agaaccccag
841 aagaggaaaa gctccatcga gatgtgttct tgccctcagt ggacaatatg aagctgcctg
901 agatgacagc tccactgccg cccctgagtg gcctggccct cttcctcatc gtctttttct
961 ccctggtgtt ttctgtattt gccatagtca ttggtatcat actctacaac aaatggcagg
1021 aacagagccg aaagcgcttc tactgagccc tcctgctgcc accacttttg tgactgtcac
1081 ccatgaggta tggaaggagc aggcactggc ctgagcatgc agcctggaga gtgttcttgt
1141 ctctagcagc tggttgggga ctatattctg tcactggagt tttgaatgca gggaccccgc
1201 attcccatgg ttgtgcatgg ggacatctaa ctctggtctg ggaagccacc caccccaggg
1261 caatgctgct gtgatgtgcc tttccctgca gtccttccat gtgggagcag aggtgtgaag
1321 agaatttacg tggttgtgat gccaaaatca cagaacagaa tttcatagcc caggctgccg
1381 tgttgtttga ctcagaaggc ccttctactt cagttttgaa tccacaaaga attaaaaact
1441 ggtaacacca caggctttct gaccatccat tcgttgggtt ttgcatttga cccaaccctc
1501 tgcctacctg aggagctttc tttggaaacc aggatggaaa cttcttccct gccttacctt
1561 cctttcactc cattcattgt cctctctgtg tgcaacctga gctgggaaag gcatttggat
1621 gcctctctgt tggggcctgg ggctgcagaa cacacctgcg tttcactggc cttcattagg
1681 tggccctagg gagatggctt tctgctttgg atcactgttc cctagcatgg gtcttgggtc
1741 tattggcatg tccatggcct tcccaatcaa gtctcttcag gccctcagtg aagtttggct
1801 aaaggttggt gtaaaaatca agagaagcct ggaagacatc atggatgcca tggattagct
1861 gtgcaactga ccagctccag gtttgatcaa accaaaagca acatttgtca tgtggtctga
1921 ccatgtggag atgtttctgg acttgctaga gcctgcttag ctgcatgttt tgtagttacg
1981 atttttggaa tcccactttg agtgctgaaa gtgtaaggaa gctttcttct tacaccttgg
2041 gcttggatat tgcccagaga agaaatttgg cttttttttt cttaatggac aagagacagt
2101 tgctgttctc atgttccaag tctgagagca acagaccctc atcatctgtg cctggaagag
2161 ttcactgtca ttgagcagca cagcctgagt gctggcctct gtcaaccctt attccactgc
2221 cttatttgac aaggggttac atgctgctca ccttactgcc ctgggattaa atcagttaca
2281 ggccagagtc tccttggagg gcctggaact ctgagtcctc ctatgaacct ctgtagccta
2341 aatgaaattc ttaaaatcac cgatggaacc aaaaaaaaaa aaaaaaaaaa
//