LOCUS BC000300 1767 bp mRNA linear HUM 11-SEP-2007
DEFINITION Homo sapiens minichromosome maintenance complex component 2, mRNA
(cDNA clone IMAGE:2820211), partial cds.
ACCESSION BC000300
VERSION BC000300.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1767)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1767)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000300.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: c Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 9943841.
FEATURES Location/Qualifiers
source 1..1767
/db_xref="H-InvDB:HIT000085826"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2820211"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1767
/gene="MCM2"
/gene_synonym="BM28"
/gene_synonym="cdc19"
/gene_synonym="D3S3194"
/gene_synonym="MITOTIN"
/db_xref="GeneID:4171"
/db_xref="HGNC:HGNC:6944"
/db_xref="MIM:116945"
CDS <1..1089
/gene="MCM2"
/gene_synonym="BM28"
/gene_synonym="cdc19"
/gene_synonym="D3S3194"
/gene_synonym="MITOTIN"
/codon_start=1
/product="MCM2 protein"
/protein_id="AAH00300.2"
/db_xref="GeneID:4171"
/db_xref="HGNC:HGNC:6944"
/db_xref="MIM:116945"
/translation="AIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLI
DEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFS
ENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAA
EPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSI
PITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTF
ARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHN
LSAFYDSELFRMNKFSHDLKRKMILQQF"
BASE COUNT 387 a 465 c 496 g 419 t
ORIGIN
1 gccatcttca ccactggcca gggggcgtcg gctgtgggcc tcacggcgta tgtccagcgg
61 caccctgtca gcagggagtg gaccttggag gctggggccc tggttctggc tgaccgagga
121 gtgtgtctca ttgatgaatt tgacaagatg aatgaccagg acagaaccag catccatgag
181 gccatggagc aacagagcat ctccatctcg aaggctggca tcgtcacctc cctgcaggct
241 cgctgcacgg tcattgctgc cgccaacccc ataggagggc gctacgaccc ctcgctgact
301 ttctctgaga acgtggacct cacagagccc atcatctcac gctttgacat cctgtgtgtg
361 gtgagggaca ccgtggaccc agtccaggac gagatgctgg cccgcttcgt ggtgggcagc
421 cacgtcagac accaccccag caacaaggag gaggaggggc tggccaatgg cagcgctgct
481 gagcccgcca tgcccaacac gtatggcgtg gagcccctgc cccaggaggt cctgaagaag
541 tacatcatct acgccaagga gagggtccac ccgaagctca accagatgga ccaggacaag
601 gtggccaaga tgtacagtga cctgaggaaa gaatctatgg cgacaggcag catccccatt
661 acggtgcggc acatcgagtc catgatccgc atggcggagg cccacgcgcg catccatctg
721 cgggactatg tgatcgaaga cgacgtcaac atggccatcc gcgtgatgct ggagagcttc
781 atagacacac agaagttcag cgtcatgcgc agcatgcgca agacttttgc ccgctacctt
841 tcattccggc gtgacaacaa tgagctgttg ctcttcatac tgaagcagtt agtggcagag
901 caggtgacat atcagcgcaa ccgctttggg gcccagcagg acactattga ggtccctgag
961 aaggacttgg tggataaggc tcgtcagatc aacatccaca acctctctgc attttatgac
1021 agtgagctct tcaggatgaa caagttcagc cacgacctga aaaggaaaat gatcctgcag
1081 cagttctgag gccctatgcc atccataagg attccttggg attctggttt ggggtggtca
1141 gtgccctctg tgctttatgg acacaaaacc agagcacttg atgaactcgg ggtactaggg
1201 tcagggctta tagcaggatg tctggctgca cctggcatga ctgtttgttt ctccaagcct
1261 gctttgtgct tctcaccttt gggtgggatg ccttgccagt gtgtcttact tggttgctga
1321 acatcttgcc acctccgagt gctttgtctc cactcagtac cttggatcag agctgctgag
1381 ttcaggatgc ctgcgtgtgg tttaggtgtt agccttctta catggatgtc aggagagctg
1441 ctgccctctt ggcgtgagtt gcgtattcag gctgcttttg ctgcctttgg ccagagagct
1501 ggttgaagat gtttgtaatc gttttcagtc tcctgcaggt ttctgtgccc ctgtggtgga
1561 agagggcacg acagtgccag cgcagcgttc tgggctcctc agtcgcaggg gtgggatgtg
1621 agtcatgcgg attatccact cgccacagtt atcagctgcc attgctccct gtctgtttcc
1681 ccactctctt atttgtgcat tcggtttggt ttctgtagtt ttaattttta ataaagttga
1741 ataaaatata aaaaaaaaaa aaaaaaa
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