LOCUS BC000297 2068 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
(soluble), mRNA (cDNA clone IMAGE:2819708), partial cds.
ACCESSION BC000297
VERSION BC000297.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2068)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2068)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 20, 2003 this sequence version replaced BC000297.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: a Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 54020719.
FEATURES Location/Qualifiers
source 1..2068
/db_xref="H-InvDB:HIT000029496"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:2819708"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2068
/gene="HMGCS1"
/db_xref="GeneID:3157"
/db_xref="HGNC:HGNC:5007"
/db_xref="MIM:142940"
CDS <1..1668
/gene="HMGCS1"
/codon_start=1
/product="HMGCS1 protein"
/protein_id="AAH00297.2"
/db_xref="GeneID:3157"
/db_xref="HGNC:HGNC:5007"
/db_xref="MIM:142940"
/translation="HSLSSAAARSRLCPKEETVTDLETAVLYPSHSSFTMPGSLPLNA
EACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINS
LCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDT
TNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGP
NAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIH
AQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLE
AFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVL
AQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSR
TGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPN
DDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH"
BASE COUNT 605 a 392 c 484 g 587 t
ORIGIN
1 cactcccttt cctctgctgc cgctcggtca cgcttgtgcc cgaaggagga aacagtgaca
61 gacctggaga ctgcagttct ctatccttca cacagctctt tcaccatgcc tggatcactt
121 cctttgaatg cagaagcttg ctggccaaaa gatgtgggaa ttgttgccct tgagatctat
181 tttccttctc aatatgttga tcaagcagag ttggaaaaat atgatggtgt agatgctgga
241 aagtatacca ttggcttggg ccaggccaag atgggcttct gcacagatag agaagatatt
301 aactctcttt gcatgactgt ggttcagaat cttatggaga gaaataacct ttcctatgat
361 tgcattgggc ggctggaagt tggaacagag acaatcatcg acaaatcaaa gtctgtgaag
421 actaatttga tgcagctgtt tgaagagtct gggaatacag atatagaagg aatcgacaca
481 actaatgcat gctatggagg cacagctgct gtcttcaatg ctgttaactg gattgagtcc
541 agctcttggg atggacggta tgccctggta gttgcaggag atattgctgt atatgccaca
601 ggaaatgcta gacctacagg tggagttgga gcagtagctc tgctaattgg gccaaatgct
661 cctttaattt ttgaacgagg gcttcgtggg acacatatgc aacatgccta tgatttttac
721 aagcctgata tgctatctga atatcctata gtagatggaa aactctccat acagtgctac
781 ctcagtgcat tagaccgctg ctactctgtc tactgcaaaa agatccatgc ccagtggcag
841 aaagagggaa atgataaaga ttttaccttg aatgattttg gcttcatgat ctttcactca
901 ccatattgta aactggttca gaaatctcta gctcggatgt tgctgaatga cttccttaat
961 gaccagaata gagataaaaa tagtatctat agtggcctgg aagcctttgg ggatgttaaa
1021 ttagaagaca cctactttga tagagatgtg gagaaggcat ttatgaaggc tagctctgaa
1081 ctcttcagtc agaaaacaaa ggcatcttta cttgtatcaa atcaaaatgg aaatatgtac
1141 acatcttcag tatatggttc ccttgcatct gttctagcac agtactcacc tcagcaatta
1201 gcagggaaga gaattggagt gttttcttat ggttctggtt tggctgccac tctgtactct
1261 cttaaagtca cacaagatgc tacaccgggg tctgctcttg ataaaataac agcaagttta
1321 tgtgatctta aatcaaggct tgattcaaga actggtgtgg caccagatgt cttcgctgaa
1381 aacatgaagc tcagagagga cacccatcat ttggtcaact atattcccca gggttcaata
1441 gattcactct ttgaaggaac gtggtactta gttagggtgg atgaaaagca cagaagaact
1501 tacgctcggc gtcccactcc aaatgatgac actttggatg aaggagtagg acttgtgcat
1561 tcaaacatag caactgagca tattccaagc cctgccaaga aagtaccaag actccctgcc
1621 acagcagcag aacctgaagc agctgtcatt agtaatgggg aacattaaga tactctgtga
1681 ggtgcaagac ttcagggtgg ggtgggcatg gggtgggggt atgggaacag ttggaggaat
1741 gggatatctg gggataattt taaaggatta catgttatgt aaatttttat gtgactgaca
1801 tggagcctgg atgactatcg tgtacttggg aaagtctctt tgctctattt gctgacatgc
1861 ttcctgttgt ggtctggcca atgccaaatg tactcgaatg atgttaaggg ctctgtaaaa
1921 cttcatacct ctttggccat ttgtatgcat gatgtttggt ttttaaacat ggtataatga
1981 attgtgtact tctgtcagaa gaaagcagag gtactaatct ccaattaaaa aattttttaa
2041 catgtaaaaa aaaaaaaaaa aaaaaaaa
//