LOCUS BC000285 2501 bp mRNA linear HUM 10-MAY-2004
DEFINITION Homo sapiens polymerase (RNA) III (DNA directed) polypeptide E
(80kD), mRNA (cDNA clone MGC:957 IMAGE:3356711), complete cds.
ACCESSION BC000285
VERSION BC000285.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2501)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2501)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steve Jones, Sarah Barber, Mabel Brown-John, Yaron Butterfield,
Andy Chan, Steve S. Chand, William Chow, Alison Cloutier, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Amara Masson, Mike R. Mayo, Josh Moran, Ryan Morin,
Teika Olson, Diana Palmquist, Anca Petrescu, Anna Liisa Prahbu,
Parvaneh Saeedi, JR Santos, Angelique Schnerch, Ursula Skalska,
Duane Smailus, Jeff Stott, Miranda Tsai, George Yang, Jacquie
Schein, Asim Siddiqui, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: i Column: 10
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8922476.
FEATURES Location/Qualifiers
source 1..2501
/db_xref="H-InvDB:HIT000029488"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:957 IMAGE:3356711"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2501
/gene="POLR3E"
/gene_synonym="RPC5"
/gene_synonym="SIN"
/db_xref="GeneID:55718"
CDS 144..2144
/gene="POLR3E"
/gene_synonym="RPC5"
/gene_synonym="SIN"
/codon_start=1
/product="POLR3E protein"
/protein_id="AAH00285.1"
/db_xref="GeneID:55718"
/translation="MANEEDDPVVQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPH
LSAKIKPKQQKVELEMAIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTF
CSSQTTSNTSRYAAALYRQGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGD
SSQDEAEDDVKQITVRFSRPESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGLRDSRS
EHERQYLLCPGSSGVENTELVKSPSEYLMMLMPPSQEEEKDKPVAPSNVLSMAQLRTL
PLADQIKILMKNVKVMPFANLMSLLGPSIDSVAVLRGIQKVAMLVQGNWVVKSDILYP
KDSSSPHSGVPAEVLCRGRDFVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHMAVV
RINKGWEFILPYDGEFIKKHPDVVQRQHMLWTGIQAKLEKVYNLVKETMPKKPDAQSG
PAGLVCGDQRIQVAKTKAQQNHALLERELQRRKEQLRVPAVPPGVRIKEEPVSEEGEE
DEEQEAEEEPMDTSPSGLHSKLANGLPLGRAAGTDSFNGHPPQGCASTPVARELKAFV
EATFQRQFVLTLSELKRLFNLHLASLPPGHTLFSGISDRMLQDTVLAAGCKQILVPFP
PQTAASPDEQKVFALWESGDMSDQDCCVSYGGMWYLKGTVQS"
BASE COUNT 602 a 692 c 754 g 453 t
ORIGIN
1 cagcaacatg gccgccgcct gagaggagag ccgggccgcc gccgtctctg cagcccgcgg
61 gtaactgggc cgttgccgcc gtccgcgctc ggcccccgcg gagagatcga gctgaaggac
121 tgcgcggctg gctctcctct agtatggcca atgaagagga tgacccagtt gtacaggaga
181 tcgatgtgta cttggccaag agtctggcgg aaaagctgta tctatttcag taccctgtgc
241 gtccagcctc gatgacctac gatgacattc cgcacctctc agccaagatc aagcccaagc
301 agcagaaggt agagcttgag atggccatcg acaccctgaa ccccaactat tgccgcagca
361 aaggggagca gattgcgctg aacgtggacg gggcctgcgc cgacgagacc agcacgtatt
421 cctcgaagct gatggacaag cagaccttct gctcttccca gaccaccagt aacacatccc
481 gttatgccgc tgcactctac aggcaaggtg agctccacct gacaccttta catggcatcc
541 tgcagctgcg gcccagcttc tcctacctgg ataaggctga cgccaagcac cgggagaggg
601 aggcggccaa cgaggcaggg gactcttcac aggatgaggc ggaagacgat gttaagcaga
661 tcacggtgcg gttctcccgg ccggagtcag agcaggcccg ccagcgccgt gtgcagtcct
721 atgagttcct gcagaagaag cacgcagagg agccctgggt ccacctgcat tactatggcc
781 tgagggacag tcgctctgag catgagcgtc agtacctgct gtgccccggc tcaagcgggg
841 tggagaacac ggagctcgtc aagtcaccca gtgagtacct gatgatgctg atgccaccca
901 gccaggagga ggagaaagac aagcctgtgg cccccagcaa cgtcctgtcg atggcccagc
961 tgcgcacgct gcccctggcc gatcagatca agatcctgat gaagaatgtg aaggtcatgc
1021 cttttgccaa cttgatgagc ctcctgggcc cctccatcga ttccgtggct gttctgcggg
1081 gcatccagaa ggtggcgatg ttggtccaag ggaactgggt ggtgaagagt gacatcctat
1141 accccaagga ctcgtccagc cctcacagcg gcgtgcctgc tgaggtgctc tgcaggggcc
1201 gagacttcgt tatgtggaag ttcacgcaga gccgctgggt ggttaggaaa gaggtggcaa
1261 ccgtgaccaa actctgcgcc gaggatgtga aggacttcct ggagcacatg gccgtggtga
1321 ggatcaacaa aggctgggag ttcattctgc cttatgatgg ggagttcatc aagaagcacc
1381 cggatgtggt ccagcggcag cacatgctgt ggacgggtat ccaggccaaa ctggaaaaag
1441 tctataatct tgtaaaggaa accatgccaa agaagccgga tgcacaatca gggcctgccg
1501 ggctggtctg tggggaccag cggatccaag tagccaaaac caaggcccag cagaaccacg
1561 cgttgctgga gcgggagctg cagcggcgga aggagcagct gcgggtgcct gcggtcccgc
1621 ccggtgtgcg gatcaaggag gagcccgtga gcgaggaggg cgaggaggac gaggagcagg
1681 aggcggagga ggagcccatg gacacttccc ccagcggcct ccacagcaag ctggccaacg
1741 ggctgcctct cgggcgggct gcgggcacag acagcttcaa cgggcacccg ccccagggct
1801 gcgccagcac ccctgtggct cgggaactga aggccttcgt ggaggccacc tttcagagac
1861 agtttgtgct cacgctgagc gaactcaagc gcctcttcaa tctgcacttg gccagcctgc
1921 cccccggcca cacactcttc agcggcatct cggaccgcat gctacaggac acggtgctgg
1981 ccgccggttg caagcagata ctggtgcctt ttccccccca gactgctgct tccccggatg
2041 agcagaaggt gtttgccctc tgggagtctg gagacatgag tgatcaggac tgctgtgtaa
2101 gctatggtgg catgtggtac cttaaaggga cagtacagtc ttgacaatag tagcaaacta
2161 ctaacccagc aaatctaagc ccaaggaaga agggcggaac cagaagtagg gcctcgactt
2221 gcttcagacg acacagagca agaggaactg accatctcat gacctgtggc attgcacggt
2281 gcagtggaca gaagggatta tcctcagcca gtcacagggt cagcttaagt tagttagatc
2341 actcccagaa gagaccagct gggaccttct ttgcagtaca atttgaaatt cctgatgtat
2401 tttgcttatt atttggtttc attctcataa taaagagagt gtatactgac atgggcagga
2461 tgataaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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