LOCUS BC000272 2575 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 50, mRNA (cDNA
clone MGC:3199 IMAGE:3357684), complete cds.
ACCESSION BC000272
VERSION BC000272.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2575)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2575)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: o Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 13129005.
FEATURES Location/Qualifiers
source 1..2575
/db_xref="H-InvDB:HIT000029479"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3199 IMAGE:3357684"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2575
/gene="DDX50"
/gene_synonym="GU2"
/gene_synonym="GUB"
/gene_synonym="MGC3199"
/gene_synonym="RH-II/GuB"
/db_xref="GeneID:79009"
/db_xref="HGNC:HGNC:17906"
CDS 108..2321
/gene="DDX50"
/gene_synonym="GU2"
/gene_synonym="GUB"
/gene_synonym="MGC3199"
/gene_synonym="RH-II/GuB"
/codon_start=1
/product="DEAD (Asp-Glu-Ala-Asp) box polypeptide 50"
/protein_id="AAH00272.1"
/db_xref="GeneID:79009"
/db_xref="HGNC:HGNC:17906"
/translation="MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEK
SETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDID
EYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYL
FPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLA
PTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQS
GRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVY
KVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRA
IIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAAR
GLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAG
ITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAAL
AHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMC
LLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGW
SSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD"
BASE COUNT 836 a 478 c 603 g 658 t
ORIGIN
1 gcatggccaa ggccgggcgg ccgccttgcc cccgcttcct ttcacgctgt cgctgcccgt
61 aggtggttgt ggccactgtg cccggaggga ggcggcggtg gccagtaatg cctgggaaac
121 tcctctgggg ggacattatg gagctggaag cacccttgga ggagtccgag agccagaaga
181 aggagaggca aaagagtgac agaaggaagt caaggcacca ttatgactcg gatgagaaat
241 cagaaacaag agaaaatggt gttacagatg acctggatgc tcccaaggcc aaaaaatcta
301 aaatgaaaga gaagctaaat ggagacactg aagaaggatt taatagactt tcagatgaat
361 tctccaaatc tcataagtca agaagaaaag atctaccaaa tggagatata gatgaatatg
421 aaaaaaaatc aaagcgagta tcatctttag atacttctac tcataaatca agtgataata
481 aactagagga gaccttaaca cgtgaacaga aagaaggagc cttctccaat tttcctattt
541 ctgaagagac tataaagctt ctgaaaggtc gaggggtaac atatctcttt cctattcaag
601 ttaagacctt tggtcctgta tatgaaggaa aagatttaat agctcaagca cggacaggaa
661 caggaaagac attctctttt gccatcccct taattgaaag actccaaaga aatcaagaaa
721 caattaaaaa aagccgctca ccaaaggtac ttgttttggc tccaacaagg gaactggcaa
781 accaagtagc caaagacttc aaagatataa ctaggaaact cagcgtggcg tgtttttatg
841 gtggaacatc atatcaaagc caaattaatc atattcgaaa tggtattgac atcttggttg
901 gaacacctgg tcgtatcaaa gaccatctgc agagtggccg attggatctt tctaaactgc
961 gacatgttgt gcttgatgaa gtggatcaga tgttagattt aggtttcgct gaacaagttg
1021 aagatattat tcatgaatcc tacaaaactg attctgaaga caatcctcag actttacttt
1081 tttctgcaac ttgcccacag tgggtataca aagttgcaaa aaaatacatg aaatccagat
1141 atgaacaggt tgaccttgtt ggaaaaatga ctcaaaaggc tgcaactact gtggaacatt
1201 tggccatcca gtgtcattgg tctcagaggc cagcagttat tggagatgtc cttcaagtct
1261 acagtgggtc tgaagggagg gctattattt tctgtgagac caagaagaat gtaactgaaa
1321 tggccatgaa tccacacata aaacagaatg cccagtgttt acatggggac attgcacagt
1381 cacaaagaga aattacacta aaaggcttca gagaaggtag ttttaaagtt ttggtggcaa
1441 ccaatgtggc tgcccgtggt ttggacattc ctgaagttga cctggtgatt caaagttctc
1501 ctcctcagga tgttgagtcc tatatccatc gctctggacg cacaggtaga gctggacgga
1561 cagggatttg tatatgtttt tatcaaccaa gagaaagagg tcaactaaga tatgtggaac
1621 aaaaagcagg aattactttt aaacgtgtag gtgttccttc tacaatggat ttagttaaat
1681 ctaaaagcat ggatgccatc aggtctctgg cttccgtttc ttatgctgct gttgattttt
1741 tccgaccatc agctcagaga ctgatagaag agaaaggtgc agtggatgca ttggctgcag
1801 ctttagccca catttctggt gcatcaagct ttgaaccacg atctttgatc acctctgata
1861 aggggtttgt gaccatgact ctggaaagcc tagaggaaat acaggatgtc agctgtgctt
1921 ggaaagaact taacagaaag ctgagtagta atgcagtgtc tcagattacc agaatgtgcc
1981 tcctgaaagg aaatatgggt gtttgctttg atgttcctac aactgagtca gaaaggttac
2041 aggcagagtg gcatgattcc gactggatac tctcagtgcc agccaaatta cctgaaattg
2101 aagaatatta tgatggaaac acatcttcta attccagaca gaggagtggc tggtcaagtg
2161 gtcgatcagg ccggtcaggc cggtcaggtg gtcgatctgg cggccggtca ggtagacaga
2221 gtcgacaagg aagtcgctca ggaagtcgac aagatggtag aagacgaagt gggaatagaa
2281 atcgatcaag aagtgggggc cacaaacgga gttttgactg agtatttgat agttaatcta
2341 ccagtgtgag cttgcctatt tctgcctaat catgtacatt atccaccaaa aattaggtca
2401 tcatagttga ggtatgtgtc tgctatttgc aaagaagttg gtcgtatttt tttaaaaagt
2461 atttcacaag aatggaacaa atctacttat ccagttatac ctttgaataa aaaaacctcc
2521 ttttattgtc tcttttcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//