LOCUS BC000192 2851 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens dihydrofolate reductase, mRNA (cDNA clone MGC:857
IMAGE:3352539), complete cds.
ACCESSION BC000192
VERSION BC000192.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2851)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2851)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: k Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 31341213.
FEATURES Location/Qualifiers
source 1..2851
/db_xref="H-InvDB:HIT000029421"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:857 IMAGE:3352539"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2851
/gene="DHFR"
/db_xref="GeneID:1719"
/db_xref="HGNC:HGNC:2861"
/db_xref="MIM:126060"
CDS 56..619
/gene="DHFR"
/codon_start=1
/product="dihydrofolate reductase"
/protein_id="AAH00192.1"
/db_xref="GeneID:1719"
/db_xref="HGNC:HGNC:2861"
/db_xref="MIM:126060"
/translation="MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSV
EGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTE
QPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLL
PEYPGVLSDVQEEKGIKYKFEVYEKND"
BASE COUNT 870 a 586 c 640 g 755 t
ORIGIN
1 cttgaccgcg cgttctgctg taacgagcgg gctcggaggt cctcccgctg ctgtcatggt
61 tggttcgcta aactgcatcg tcgctgtgtc ccagaacatg ggcatcggca agaacgggga
121 cctgccctgg ccaccgctca ggaatgaatt cagatatttc cagagaatga ccacaacctc
181 ttcagtagaa ggtaaacaga atctggtgat tatgggtaag aagacctggt tctccattcc
241 tgagaagaat cgacctttaa agggtagaat taatttagtt ctcagcagag aactcaagga
301 acctccacaa ggagctcatt ttctttccag aagtctagat gatgccttaa aacttactga
361 acaaccagaa ttagcaaata aagtagacat ggtctggata gttggtggca gttctgttta
421 taaggaagcc atgaatcacc caggccatct taaactattt gtgacaagga tcatgcaaga
481 ctttgaaagt gacacgtttt ttccagaaat tgatttggag aaatataaac ttctgccaga
541 atacccaggt gttctctctg atgtccagga ggagaaaggc attaagtaca aatttgaagt
601 atatgagaag aatgattaat atgaaggtgt tttctagttt aagttgttcc ccctccctct
661 gaaaaaagta tgtattttta cattagaaaa ggttttttgt tgactttaga tctataatta
721 tttctaagca actagttttt attccccact actcttgtct ctatcagata ccatttatga
781 gacattcttg ctataactaa gtgcttctcc aagaccccaa ctgagtcccc agcacctgct
841 acagtgagct gccattccac acccatcaca tgtggcactc ttgccagtcc ttgacattgt
901 cgggcttttc acatgttggt aatatttatt aaagatgaag atccacatac ccttcaactg
961 agcagtttca ctagtggaaa taccaaaagc ttcctacgtg tatatccaga ggtttgtaga
1021 taaatgttgc caccttgttt gtaacagtga aaaattgaaa acaacctgga agtccagtga
1081 tgggaaaatg agtatgtttc tgtcttagat tggggaaccc aaagcagatt gcaagactga
1141 aatttcagtg aaagcagtgt atttgctagg tcataccaga aatcatcaat tgaggtacgg
1201 agaaactgaa ctgagaaggt aagaaaagca atttaaagtc agcgagcagg ttctcattga
1261 taacaagctc catactgctg agatacaggg aaatggaggg gggaaagctg gagtattgat
1321 cccgcccccc tccttggttg tcagctccct gtcctgtgtg tgggcggaac atagtccagc
1381 tgctctatag caagtctcag gtgtttgcag taagaagctg ctggcatgca cgggaacagt
1441 gaatgccaaa cacttaaagc aattcgatgt ttaagtatgt aagttctttt ttttttagac
1501 agcgtttcgc tcttgttgcc caggctggca tgcagtggtg tgacctcggc ttactgcaac
1561 ctccgccttc ccagattcaa gcgattctcc tgcctcaggc tcccaagtag ctaggaccag
1621 gtgcgcgcca ccacgcccgg ctaatttttg tattttgtat ttttagtaga gatggggttt
1681 caccatgttg gtcaggctgg tctcgaactc gtgaccgcaa gcgattcacc cacctcagcc
1741 tcccaaagtg ctgggattac cggcttgagc caccacaccc ggcacatctt cattcttttt
1801 atgtagtaaa aagtataagg ccacacatgg tttatttgaa gtattttata atttaaaaaa
1861 atacagaagc aggaaaacca attataagtt caagtgaggg atgatggttg cttgaaccaa
1921 agggttgcat gtagtaagaa attgtgattt aagatatatt ttaaagttat aagtagcagg
1981 atattctgat ggagtttgac tttggttttg ggcccaggga gtttcagatg cctttgagaa
2041 atgaatgaag tagagagaaa ataaaagaaa aaccagccag gcacagtggc tcacgcctgt
2101 aatcccagcg ctttgggagg ctaaggcagg cagatcactt gagaccagct tgggcaacat
2161 ggcaaagccc catctctaca aaaaacacaa aaattagctg ggcattgtgg cgcacacctg
2221 tattcccatc tagtcaggaa gctgagatgg aagaattaat tgagcccacg agttcaaggc
2281 tgcagtgagt cgtgattgtg ccactgcact ccagccgggg tgacagaaga gaccttgtct
2341 cgaaaaggaa tctgaaaaca atggaaccat gccttcataa ttctagaaag ttattttcaa
2401 ctgataaatc tatattcacc caaataatca agggtgaagg taaaataata catttttaga
2461 caagcaaaga ctcaggggtt acctccatgt gcccttttta gggaagctgt tggagaaaat
2521 actccagcaa aatgaaggag tacacaaacc agagaatgac atgaatccag caaataggat
2581 ccaacacagg caatattcca gctatggagc tagctttaaa aaggaacagt aaaaatatta
2641 atcggttagc tgggtggaat ggcccatgcc tgtagtccca gctactcagg aggctcagca
2701 gcaggacgac ttgagcccaa gagttccaga ccagcctggc caccttagtg agatcccttc
2761 tcttaaaaat aataacttat tgccagattt ggggcatttg gaaagaagtt cattgaagat
2821 aaagcaaaag taaaaaaaaa aaaaaaaaaa a
//