LOCUS BC000054 2614 bp mRNA linear HUM 04-OCT-2003
DEFINITION Homo sapiens 7-dehydrocholesterol reductase, mRNA (cDNA clone
MGC:1760 IMAGE:3507516), complete cds.
ACCESSION BC000054
VERSION BC000054.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2614)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2614)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC000054.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 7 Row: e Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4503320.
FEATURES Location/Qualifiers
source 1..2614
/db_xref="H-InvDB:HIT000029312"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1760 IMAGE:3507516"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2614
/gene="DHCR7"
/gene_synonym="SLOS"
/db_xref="GeneID:1717"
/db_xref="MIM:602858"
CDS 176..1603
/gene="DHCR7"
/gene_synonym="SLOS"
/codon_start=1
/product="DHCR7 protein"
/protein_id="AAH00054.1"
/db_xref="GeneID:1717"
/db_xref="MIM:602858"
/translation="MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVI
FLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTL
WVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFA
NAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYN
YMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVL
QAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLST
PHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHS
KLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHR
CASKYGRDWERYTAAVPYRLLPGIF"
misc_feature 452..1600
/gene="DHCR7"
/gene_synonym="SLOS"
/note="ERG4_ERG24; Region: Ergosterol biosynthesis
ERG4/ERG24 family"
/db_xref="CDD:pfam01222"
BASE COUNT 540 a 782 c 695 g 597 t
ORIGIN
1 gtggagcagc gcgcgcaagc gaggccaggg gaaggtgggc gcaggacttt agccggttga
61 gaaggatcaa gcaggcattt ggagcacagg tgtctagaaa cttttaaggg gccggttcaa
121 gaaggaaaag ttcccttctg ctgtgaaact atctggcaag aggctggagg gcccaatggc
181 tgcaaaatcg caacccaaca ttcccaaagc caagagtcta gatggcgtca ccaatgacag
241 aaccgcatct caagggcagt ggggccgtgc ctgggaggtg gactggtttt cactggcgag
301 cgtcatcttc ctactgctgt tcgccccctt catcgtctac tacttcatca tggcttgtga
361 ccagtacagc tgcgccctga ccggccctgt ggtggacatc gtcactggac atgctcggct
421 ctcggacatc tgggccaaga ctccacctat aacgaggaaa gccgcccagc tctatacctt
481 gtgggtcacc ttccaggtgc ttctgtacac gtctctccct gacttctgcc ataagtttct
541 acccggctac gtaggaggca tccaggaggg ggccgtgact cctgcagggg ttgtgaacaa
601 gtatcagatc aacggcctgc aagcctggct cctcacgcac ctgctctggt ttgcaaacgc
661 tcatctcctg tcctggttct cgcccaccat catcttcgac aactggatcc cactgctgtg
721 gtgcgccaac atccttggct atgccgtctc caccttcgcc atggtcaagg gctacttctt
781 ccccaccagc gccagagact gcaaattcac aggcaatttc ttttacaact acatgatggg
841 catcgagttt aaccctcgga tcgggaagtg gtttgacttc aagctgttct tcaatgggcg
901 ccccgggatc gtcgcctgga ccctcatcaa cctgtccttc gcagcgaagc agcgggagct
961 ccacagccat gtgaccaatg ccatggtcct ggtcaacgtc ctgcaggcca tctacgtgat
1021 tgacttcttc tggaacgaaa cctggtacct gaagaccatt gacatctgcc atgaccactt
1081 cgggtggtac ctgggctggg gcgactgtgt ctggctgcct tatctttaca cgctgcaggg
1141 tctgtacttg gtgtaccacc ccgtgcagct gtccaccccg cacgccgtgg gcgtcctgct
1201 gctgggcctg gtgggctact acatcttccg ggtggccaac caccagaagg acctgttccg
1261 ccgcacggat gggcgctgcc tcatctgggg caggaagccc aaggtcatcg agtgctccta
1321 cacatccgcc gacgggcaga ggcaccacag caagctgctg gtgtcgggct tctggggcgt
1381 ggcccgccac ttcaactacg tcggcgacct gatgggcagc ctggcctact gcctggcctg
1441 tggcggcggc cacctgctgc cctacttcta catcatctac atggccatcc tgctgaccca
1501 ccgctgcctc cgggacgagc accgctgcgc cagcaagtac ggccgggact gggagcgcta
1561 caccgccgca gtgccttacc gcctgctgcc tggaatcttc taagggcacg ccctagggag
1621 aagccctgtg gggctgtcaa gagcgtgttc tgccaggtcc atgggggctg gcatcccagc
1681 tccaactcga ggagcctcag tttcctcatc tgtaaactgg agagagccca gcacttggca
1741 ggtgtccagt acctaatcac gcttttgcct tcaagggaat tccgagtgtc cagcactgcc
1801 gtattgccag cacagacgga ttttctctaa tcagtgtccc tggggcagga ggatgaccca
1861 gtcaccttta ctagtccttt ggagacaatt tacctgtatt aggagcccag gccacgctac
1921 actctgccca cactggtgag caggaggtct tcccacgccc tgtcattagg ctgcatttac
1981 tcttgctaaa taaaagtggg agtggggcgt gcgcgttatc catgtattgc ctttcagctc
2041 tagatccccc tcccctgcct gctctgcagt tgtgggtggg gcccgtgcgc cgtttctcct
2101 tggtagcgtg cacggtgttg aactgggaca ctggggagaa aggggctttc atgtcgtttc
2161 cttcctgctc ctgctgcaca gctgccagga gtgctctgcc tggagtctgc agacctcaga
2221 gaggtcccag cactggctgt ggcctttcag gtgtaggcag gtgggctctg cttcccgatt
2281 ccctgtgagc gcccaccctc tcgaaagaat tttctgcttg ccctgtgact gtgcagactc
2341 tggctcgagc aacccgggga acttcaccct caggggcctc ccacaccttc tccagcgagg
2401 aggtctcagt cccagcctcg ggagggcacc tccttttctg tgctttcttc cctgaggcat
2461 tcttcctcat ccctagggtg ttgtgtagaa ctctttttaa actctatgct ccgagtagag
2521 ttcatcttta tattaaactt cccctgttca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//