LOCUS       LC732346                 546 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Rattus rattus DNA, 3'arm side boundary of tetO-H2B tdTomato/Rosa26
            knock-in rat.
ACCESSION   LC732346
VERSION     LC732346.1
KEYWORDS    .
SOURCE      Rattus rattus (black rat)
  ORGANISM  Rattus rattus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..546
                     /chromosome="4"
                     /collection_date="2022-05-20"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10117"
                     /mol_type="genomic DNA"
                     /note="3'arm side boundary of tetO-H2B tdTomato/Rosa26
                     knock-in rat"
                     /organism="Rattus rattus"
                     /tissue_type="tail"
                     /transgenic
     source          1..514
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with TRE3G promoter, H2B, tdTomato,
                     beta globin and polyA"
                     /organism="synthetic construct"
     misc_feature    515..>546
                     /note="endogenous region of acceptor, flanking region of
                     Thumpd3-as1 gene, Lister Hooded strain"
BASE COUNT          150 a          115 c          117 g          164 t
ORIGIN      
        1 gggagtcttc tgggcaggct taaaggctaa cctggtgcgt ggggcgttgt cctgcagagg
       61 aattgaacag gtgtaaaatt ggaggggcaa gacttcccac agattttcga ttgtgttgtt
      121 aagtattgta ataggggcaa ataagggaaa tagactaggc actcacctgg ggttttatgc
      181 agcaaaacta caggttatta ttgcttgtga tccgccctgg agaatttttc accgaggtag
      241 attgaagaca tgcccaccca aattttaata ttcttccact tgcgatcctt gctacagtat
      301 gaaattacag tatcgtgaat tagaatatat aagcagaatt ttaagcattt taaaagagcc
      361 cagtacttca tgtctgtctc tcccacttct gcagccctat caaagggtat tttagcacac
      421 tcattttagc cccattttca tttgttgtac tggcttatcc aatccctaga cagagcactg
      481 gcattccctc tctcctgatc ttagaagtcc gatgactcat gaaaccagac agattagttt
      541 cataca
//