LOCUS       LC732345                 512 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Rattus rattus DNA, 5'arm side boundary of tetO-H2B tdTomato/Rosa26
            knock-in rat.
ACCESSION   LC732345
VERSION     LC732345.1
KEYWORDS    .
SOURCE      Rattus rattus (black rat)
  ORGANISM  Rattus rattus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..512
                     /chromosome="4"
                     /collection_date="2022-05-20"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10117"
                     /mol_type="genomic DNA"
                     /note="5'arm side boundary of tetO-H2B tdTomato/Rosa26
                     knock-in rat"
                     /organism="Rattus rattus"
                     /tissue_type="tail"
                     /transgenic
     source          1..487
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with TRE3G promoter, H2B, tdTomato,
                     beta globin and polyA"
                     /organism="synthetic construct"
     misc_feature    488..>512
                     /note="endogenous region of acceptor, flanking region of
                     Thumpd3-as1 gene, Lister Hooded strain"
BASE COUNT          105 a          177 c          143 g           87 t
ORIGIN      
        1 gatcacgagg gaagaagggg gaagggaatc ttccaggccc agggcggtcc tcagaagaca
       61 ggaggcagca gagaactccc agaaaggtat tgcaacactc ccctcccccg tcctgagaag
      121 ggtgcggcct tttctccgcc tactccactg cagctccctt aatgataact caaaccgact
      181 tttggagagc aagtgcttct tgtctccaat acaacccgac tgagccccaa ctccttcatc
      241 cactccctgg agtgcaatgt aggtcttgtt cagcacgttc ctcagtgcca caactcaagg
      301 aggaatcccg tttgcacccg cccgccgctc agaccgaccg cccgtccccg cccccagcgc
      361 gcgcttcctg ccacgttgcg caggggcgcg gggccagact ctgcggcgcg gggcctgggg
      421 ggagggccgg aacctgtgag cgcctccctg ccgcccccgc cagccagctt agggactgaa
      481 cttgcatgaa cgcgggccaa ttgcaaaggc ca
//