LOCUS       LC732344                 658 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Mus musculus DNA, 3'arm side boundary of ELKS-mCherry knock-in
            mouse.
ACCESSION   LC732344
VERSION     LC732344.1
KEYWORDS    .
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..658
                     /chromosome="6"
                     /collection_date="2020-08-19"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10090"
                     /mol_type="genomic DNA"
                     /note="3'arm side boundary of ELKS-mCherry knock-in mouse"
                     /organism="Mus musculus"
                     /tissue_type="tail"
                     /transgenic
     source          1..417
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with ELKS and mCherry"
                     /organism="synthetic construct"
     misc_feature    418..>658
                     /note="endogenous region of acceptor, flanking region of
                     Erc1 gene, C57BL/6N strain"
BASE COUNT          163 a          137 c          152 g          206 t
ORIGIN      
        1 gcctggtcag agagagtgct aaggcaacca ggggtacaca gagaaatcat ctcttgaaaa
       61 acaacaaaaa caaaaaatca aagggcttct taatgataca gttgcatttg agtcacctct
      121 gcctctgtaa gtagcccctc agtcatagcc tttattttat ttatattggt gttaggtttt
      181 gtctctgtgt atgtcggttt gagggtgtca gatcctctgg aactggagtt acagacaatt
      241 gtgagctgct gtgtgggtgc tgggatttga acctgggtcc tctggaagaa caactagtgt
      301 tcttaactgc tgagctgagc catctctcta gtcccataag agcaggatat tttgttggtt
      361 tgcttgcttg ttttggtttt tgagacacag ttctctgagt aacaccgggc tgtcctggaa
      421 ctcactctgt agaccaggct ggcctagaac tcacagagat cttcctgccc ctgcctcctg
      481 agtgctggga ttaaaggagt gggtcactgt gtttggctcc cctctcccat atttttctaa
      541 gtcacctcaa taaaaagtat tcattggcat accaaagtaa gtaaaaggtt catattttta
      601 aaaacaattt aaagtcaatt cagcacttct ctcttgcttc actgtgtggt gtgggctg
//