LOCUS       LC732343                 380 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Mus musculus DNA, 5'arm side boundary of ELKS-mCherry knock-in
            mouse.
ACCESSION   LC732343
VERSION     LC732343.1
KEYWORDS    .
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..380
                     /chromosome="6"
                     /collection_date="2020-07-30"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10090"
                     /mol_type="genomic DNA"
                     /note="5'arm side boundary of ELKS-mCherry knock-in mouse"
                     /organism="Mus musculus"
                     /tissue_type="tail"
                     /transgenic
     source          1..314
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with ELKS and mCherry"
                     /organism="synthetic construct"
     misc_feature    315..>380
                     /note="endogenous region of acceptor, flanking region of
                     Erc1 gene, C57BL/6N strain"
BASE COUNT          146 a           69 c           67 g           98 t
ORIGIN      
        1 tgctttttgt gtacactgag catacgtatc aagtatttca tcaataaaat aatgtacatc
       61 aacaccggat actgtacaat acccaataat gttctaagta aaattctggg aaacaaagtc
      121 ccatcagatc atcatattct cataaagaac tacagacttt attataaaag cagacatgag
      181 acatgtttat aatcctagct acagggagtc tgaggcagat aaggccagct tgagctacgt
      241 ctcaaaatat aagatgaaat aaaataaaca aaacaaataa accagacaga cagggttgta
      301 tctgcttgta accccaagca ttggggaaga tgaagcaaga ggttatcaag ttcaaggcca
      361 atgtgagctt ttacaaattc
//