LOCUS       LC732342                 284 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Mus musculus DNA, 3'arm side boundary of CAG-EGFP/Rosa26 knock-in
            mouse.
ACCESSION   LC732342
VERSION     LC732342.1
KEYWORDS    .
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..284
                     /chromosome="6"
                     /collection_date="2021-05-12"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10090"
                     /mol_type="genomic DNA"
                     /note="3'arm side boundary of CAG-EGFP/Rosa26 knock-in
                     mouse"
                     /organism="Mus musculus"
                     /tissue_type="tail"
                     /transgenic
     source          1..278
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with CAG promoter, EGFP, beta globin
                     and polyA"
                     /organism="synthetic construct"
     misc_feature    279..>284
                     /note="endogenous region of acceptor, flanking region of
                     Gt(ROSA)26Sor gene, ICR strain"
BASE COUNT           75 a           69 c           44 g           96 t
ORIGIN      
        1 tgatccgcct cggagtattt tccatcgagg tagattaaag acatgctcac ccgagtttta
       61 tactctcctg cttgagatcc ttactacagt atgaaattac agtgtcgcga gttagactat
      121 gtaagcagaa ttttaatcat ttttaaagag cccagtactt catatccatt tctcccgctc
      181 cttctgcagc cttatcaaaa ggtattttag aacactcatt ttagccccat tttcatttat
      241 tatactggct tatccaaccc ctagacagag cattggcatt ttcc
//