LOCUS       LC732341                 367 bp    DNA     linear   SYN 18-OCT-2022
DEFINITION  Mus musculus DNA, 5'arm side boundary of CAG-EGFP/Rosa26 knock-in
            mouse.
ACCESSION   LC732341
VERSION     LC732341.1
KEYWORDS    .
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Abe,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) to the DDBJ/EMBL/GenBank databases.
            Contact:Manabu Abe
            Brain Research Institute, Niigata University, Department of Animal
            Model Development; 1-757 Asahimachidori, Chuo-ku, Niigata, Niigata
            951-8585, Japan
            URL    :https://www.bri.niigata-u.ac.jp/field/cellular/index.html
REFERENCE   2  
  AUTHORS   Abe,M., Nakatsukasa,E., Natsume,R., Hamada,S., Sakimura,K.,
            Watabe,A.M. and Ohtsuka,T.
  TITLE     A novel technique for large-fragment knock-in animal production
            without ex vivo handling of zygotes
  JOURNAL   Unpublished (2022)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..367
                     /chromosome="6"
                     /collection_date="2021-05-12"
                     /country="Japan: Niigata"
                     /db_xref="taxon:10090"
                     /mol_type="genomic DNA"
                     /note="5'arm side boundary of CAG-EGFP/Rosa26 knock-in
                     mouse"
                     /organism="Mus musculus"
                     /tissue_type="tail"
                     /transgenic
     source          1..357
                     /db_xref="taxon:32630"
                     /mol_type="genomic DNA"
                     /note="constucted with CAG promoter, EGFP, beta globin
                     and polyA"
                     /organism="synthetic construct"
     misc_feature    358..>367
                     /note="endogenous region of acceptor, flanking region of
                     Gt(ROSA)26Sor gene, ICR strain"
BASE COUNT           57 a          149 c          102 g           59 t
ORIGIN      
        1 cttctccccg cctactccac tgcagctccc ttactgataa caactcagag cgactttggg
       61 agagcaagtg cttcctgcct ccaaaacagc ccaactgagc cctcgctcct tccctccact
      121 ccccggagtg cgcgatggag gtctggctca gcacgcccct cttgaggcaa ctcaagtcgg
      181 aaacgtgctt gcacccgccc cgcagccgct cagccctact gcccgtcccc gcccccagcg
      241 cgcgcttcct gccacgttgc gcaggggcgc gcggccagac tctgcggcgc ggggccgagg
      301 ggagggccgg aacctgggag cgcctcctcg ccgcccccgc tggccggcgg atggactcaa
      361 cttgcac
//