LOCUS MT941067 29800 bp RNA linear VRL 31-AUG-2020 DEFINITION Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/IND/GBRC348a/2020, complete genome. ACCESSION MT941067 VERSION MT941067.1 DBLINK BioProject: PRJNA625669 BioSample: SAMN15903666 Sequence Read Archive: SRR12517247 KEYWORDS . SOURCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ORGANISM Severe acute respiratory syndrome coronavirus 2 Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Sarbecovirus. REFERENCE 1 (bases 1 to 29800) AUTHORS Kapadia,S., Puvar,A., Raval,J., Patel,Z., Gandhi,M., Trivedi,P., Pandya,M., Patel,N., Savaliya,N., Kumar,R., Kumar,D., Saiyed,Z., Patel,K., Pandya,L., Ansari,A., Trivedi,N., Shah,P., Upadhyay,K.J., Dixit,R.D., Kadri,A.M., Bakshi,H., Joshi,C. and Joshi,M. TITLE Novel Coronavirus genome sequence and its characterization from the patient in Gujarat, India during COVID-19 outbreak JOURNAL Unpublished REFERENCE 2 (bases 1 to 29800) AUTHORS Kapadia,S., Puvar,A., Raval,J., Patel,Z., Gandhi,M., Trivedi,P., Pandya,M., Patel,N., Savaliya,N., Kumar,R., Kumar,D., Saiyed,Z., Patel,K., Pandya,L., Ansari,A., Trivedi,N., Shah,P., Upadhyay,K.J., Dixit,R.D., Kadri,A.M., Bakshi,H., Joshi,C. and Joshi,M. TITLE Direct Submission JOURNAL Submitted (31-AUG-2020) Department of Science and Technology, Gujarat Biotechnology Research Centre, MS Building, Block B & D, 6th Floor, GH Road, Sector - 11, Gandhinagar, Gujarat 382011, India COMMENT ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 12 Sequencing Technology :: IonTorrent ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..29800 /organism="Severe acute respiratory syndrome coronavirus 2" /mol_type="genomic RNA" /isolate="SARS-CoV-2/human/IND/GBRC348a/2020" /host="Homo sapiens" /db_xref="taxon:2697049" /clone="CAW132" /country="India: Ahmedabad" /collection_date="2020-06-18" gene 224..21513 /gene="ORF1ab" CDS join(224..13426,13426..21513) /gene="ORF1ab" /ribosomal_slippage /codon_start=1 /product="ORF1ab polyprotein" /protein_id="QNL90918.1" /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGNSVEEVLSEARQ HLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGE TLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQ LDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFP LNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTG DFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESG LKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNL LEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGN FKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAA ITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKL KPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLV NKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEII FLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEK YCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEK CSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEF KLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPE EEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSG YLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESD DYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLA PLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIA EIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGN LHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKV PTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREM LAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLIN TLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSS KTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVIT FDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPH NSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIK WADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELG DVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQ IPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSK ETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYY KKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVT FFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWST KPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGD IILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNS VPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRI KASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTA ALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLET IQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWL MWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVE CTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRP INPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPI NVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVN TFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVEC LKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALI WNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWL KQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFA NKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLP RVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVA YESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSG RWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCL AYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTN DVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFE EAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHL AKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNG LWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVL KLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSC GSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVN VLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAV LDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHW LLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFL LPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTAR TVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLAR GIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYL VSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVL LSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKL CEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAK SEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALN NIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDA DSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTA CTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTP KGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAK AYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDH PNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSA DAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKD EDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQR LTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYA NLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVV DSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQ TYHPNCVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHFXX XXXXHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQ TVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDI RQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQ DALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAAT RGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLAR KHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQ AVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMM ILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCS QHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKH PNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTV LQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCD VTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDW TNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKP RPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSH TVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGK SHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKF KVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQ LPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLK AHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNA VASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSD RDLYDKLQFTSLEIPRRNVATLQAENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKT EGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEG CHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIP LMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCL CDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNA HVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADK FPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCL FWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQ LPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYN MMISAGFSLWVYKQFDTYNLWNTFTRLQSLENVAFNVVNKGHFDGQQGEVPVSIINNT VYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDY KRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEG SVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFK PRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESP FELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKV TIDYTEISFMLWCKDGHVETFYPKLQSSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGD SATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLP TGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEG FFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFL IGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKE GQINDMILSLLSKGRLIIRENNRVVISSDVLVNN" mat_peptide 224..763 /gene="ORF1ab" /product="leader protein" mat_peptide 764..2677 /gene="ORF1ab" /product="nsp2" mat_peptide 2678..8512 /gene="ORF1ab" /product="nsp3" mat_peptide 8513..10012 /gene="ORF1ab" /product="nsp4" mat_peptide 10013..10930 /gene="ORF1ab" /product="3C-like proteinase" mat_peptide 10931..11800 /gene="ORF1ab" /product="nsp6" mat_peptide 11801..12049 /gene="ORF1ab" /product="nsp7" mat_peptide 12050..12643 /gene="ORF1ab" /product="nsp8" mat_peptide 12644..12982 /gene="ORF1ab" /product="nsp9" mat_peptide 12983..13399 /gene="ORF1ab" /product="nsp10" mat_peptide join(13400..13426,13426..16194) /gene="ORF1ab" /product="RNA-dependent RNA polymerase" mat_peptide 16195..17997 /gene="ORF1ab" /product="helicase" mat_peptide 17998..19578 /gene="ORF1ab" /product="3'-to-5' exonuclease" mat_peptide 19579..20616 /gene="ORF1ab" /product="endoRNAse" mat_peptide 20617..21510 /gene="ORF1ab" /product="2'-O-ribose methyltransferase" CDS 224..13441 /gene="ORF1ab" /codon_start=1 /product="ORF1a polyprotein" /protein_id="QNL90919.1" /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGNSVEEVLSEARQ HLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGE TLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQ LDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFP LNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTG DFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESG LKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNL LEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGN FKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAA ITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKL KPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLV NKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEII FLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEK YCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEK CSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEF KLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPE EEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSG YLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESD DYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLA PLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIA EIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGN LHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKV PTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREM LAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLIN TLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSS KTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVIT FDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPH NSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIK WADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELG DVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQ IPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSK ETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYY KKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVT FFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWST KPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGD IILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNS VPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRI KASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTA ALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLET IQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWL MWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVE CTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRP INPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPI NVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVN TFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVEC LKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALI WNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWL KQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFA NKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLP RVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVA YESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSG RWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCL AYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTN DVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFE EAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHL AKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNG LWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVL KLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSC GSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVN VLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAV LDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHW LLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFL LPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTAR TVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLAR GIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYL VSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVL LSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKL CEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAK SEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALN NIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDA DSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTA CTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTP KGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAK AYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDH PNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSA DAQSFLNGFAV" mat_peptide 224..763 /gene="ORF1ab" /product="leader protein" mat_peptide 764..2677 /gene="ORF1ab" /product="nsp2" mat_peptide 2678..8512 /gene="ORF1ab" /product="nsp3" mat_peptide 8513..10012 /gene="ORF1ab" /product="nsp4" mat_peptide 10013..10930 /gene="ORF1ab" /product="3C-like proteinase" mat_peptide 10931..11800 /gene="ORF1ab" /product="nsp6" mat_peptide 11801..12049 /gene="ORF1ab" /product="nsp7" mat_peptide 12050..12643 /gene="ORF1ab" /product="nsp8" mat_peptide 12644..12982 /gene="ORF1ab" /product="nsp9" mat_peptide 12983..13399 /gene="ORF1ab" /product="nsp10" mat_peptide 13400..13438 /gene="ORF1ab" /product="nsp11" stem_loop 13434..13461 /gene="ORF1ab" /note="Coronavirus frameshifting stimulation element stem-loop 1" stem_loop 13446..13500 /gene="ORF1ab" /note="Coronavirus frameshifting stimulation element stem-loop 2" gene 21521..25342 /gene="S" CDS 21521..25342 /gene="S" /codon_start=1 /product="surface glycoprotein" /protein_id="QNL90920.1" /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFR SSVLHSTQDFFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIR GWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQ GFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFL LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITN LCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCF TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYN YLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPY RVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFG RDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAI HADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPR RARSVASHSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTM YICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFG GFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFN GLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQN VLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGA ISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMS ECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAH FPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELD SFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELG KYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSE PVLKGVKLHYT" gene 25351..26178 /gene="ORF3a" CDS 25351..26178 /gene="ORF3a" /codon_start=1 /product="ORF3a protein" /protein_id="QNL90921.1" /translation="MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFG WLIVGVALLAVFHSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLE APFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPY NSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQ LSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL" gene 26203..26430 /gene="E" CDS 26203..26430 /gene="E" /codon_start=1 /product="envelope protein" /protein_id="QNL90922.1" /translation="MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCC NIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV" gene 26481..27149 /gene="M" CDS 26481..27149 /gene="M" /codon_start=1 /product="membrane glycoprotein" /protein_id="QNL90923.1" /translation="MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNR FLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRL FARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCD IKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIA LLVQ" gene 27160..27345 /gene="ORF6" CDS 27160..27345 /gene="ORF6" /codon_start=1 /product="ORF6 protein" /protein_id="QNL90924.1" /translation="MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSL TENKYSQLDEEQPMEID" gene 27352..27717 /gene="ORF7a" CDS 27352..27717 /gene="ORF7a" /codon_start=1 /product="ORF7a protein" /protein_id="QNL90925.1" /translation="MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNS PFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQELYSPIFL IVAAIVFITLCFTLKRKTE" gene 27714..27845 /gene="ORF7b" CDS 27714..27845 /gene="ORF7b" /codon_start=1 /product="ORF7b" /protein_id="QNL90926.1" /translation="MIQLSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA" gene 27852..28217 /gene="ORF8" CDS 27852..28217 /gene="ORF8" /codon_start=1 /product="ORF8 protein" /protein_id="QNL90927.1" /translation="MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPCPIHFYSK WYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRC SFYEDFLEYHDVRVVLDFI" gene 28232..29491 /gene="N" CDS 28232..29491 /gene="N" /codon_start=1 /product="nucleocapsid phosphoprotein" /protein_id="QNL90928.1" /translation="MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQG LPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMK DLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSLRNSTPGSSRGTSPARMAGNGGDAA LALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGR RGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYT GAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTV TLLPAADLDDFSKQLQQSMSSADSTQA" gene 29516..29632 /gene="ORF10" CDS 29516..29632 /gene="ORF10" /codon_start=1 /product="ORF10 protein" /protein_id="QNL90929.1" /translation="MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT" stem_loop 29567..29602 /gene="ORF10" /note="Coronavirus 3' UTR pseudoknot stem-loop 1" stem_loop 29587..29615 /gene="ORF10" /note="Coronavirus 3' UTR pseudoknot stem-loop 2" stem_loop 29686..29726 /note="Coronavirus 3' stem-loop II-like motif (s2m)" BASE COUNT 8892 a 5471 c 5843 g 9580 t ORIGIN 1 tcgatctctt gtagatctgt tctctaaacg aactttaaaa tctgtgtggc tgtcactcgg 61 ctgcatgctt agtgcactca cgcagtataa ttaataacta attactgtcg ttgacaggac 121 acgagtaact cgtctatctt ctgcaggctg cttacggttt cgtccgtgtt gcagccgatc 181 atcagcacat ctaggttttg tccgggtgtg accgaaaggt aagatggaga gccttgtccc 241 tggtttcaac gagaaaacac acgtccaact cagtttgcct gttttacagg ttcgcgacgt 301 gctcgtacgt ggctttggaa actccgtgga ggaggtctta tcagaggcac gtcaacatct 361 taaagatggc acttgtggct tagtagaagt tgaaaaaggc gttttgcctc aacttgaaca 421 gccctatgtg ttcatcaaac gttcggatgc tcgaactgca cctcatggtc atgttatggt 481 tgagctggta gcagaactcg aaggcattca gtacggtcgt agtggtgaga cacttggtgt 541 ccttgtccct catgtgggcg aaataccagt ggcttaccgc aaggttcttc ttcgtaagaa 601 cggtaataaa ggagctggtg gccatagtta cggcgccgat ctaaagtcat ttgacttagg 661 cgacgagctt ggcactgatc cttatgaaga ttttcaagaa aactggaaca ctaaacatag 721 cagtggtgtt acccgtgaac tcatgcgtga gcttaacgga ggggcataca ctcgctatgt 781 cgataacaac ttctgtggcc ctgatggcta ccctcttgag tgcattaaag accttctagc 841 acgtgctggt aaagcttcat gcactttgtc cgaacaactg gactttattg acactaagag 901 gggtgtatac tgctgccgtg aacatgagca tgaaattgct tggtacacgg aacgttctga 961 aaagagctat gaattgcaga caccttttga aattaaattg gcaaagaaat ttgacacctt 1021 caatggggaa tgtccaaatt ttgtatttcc cttaaattcc ataatcaaga ctattcaacc 1081 aagggttgaa aagaaaaagc ttgatggctt tatgggtaga attcgatctg tctatccagt 1141 tgcgtcacca aatgaatgca accaaatgtg cctttcaact ctcatgaagt gtgatcattg 1201 tggtgaaact tcatggcaga cgggcgattt tgttaaagcc acttgcgaat tttgtggcac 1261 tgagaatttg actaaagaag gtgccactac ttgtggttac ttaccccaaa atgctgttgt 1321 taaaatttat tgtccagcat gtcacaattc agaagtagga cctgagcata gtcttgccga 1381 ataccataat gaatctggct tgaaaaccat tcttcgtaag ggtggtcgca ctattgcctt 1441 tggaggctgt gtgttctctt atgttggttg ccataacaag tgtgcctatt gggttccacg 1501 tgctagcgct aacataggtt gtaaccatac aggtgttgtt ggagaaggtt ccgaaggtct 1561 taatgacaac cttcttgaaa tactccaaaa agagaaagtc aacatcaata ttgttggtga 1621 ctttaaactt aatgaagaga tcgccattat tttggcatct ttttctgctt ccacaagtgc 1681 ttttgtggaa actgtgaaag gtttggatta taaagcattc aaacaaattg ttgaatcctg 1741 tggtaatttt aaagttacaa aaggaaaagc taaaaaaggt gcctggaata ttggtgaaca 1801 gaaatcaata ctgagtcctc tttatgcatt tgcatcagag gctgctcgtg ttgtacgatc 1861 aattttctcc cgcactcttg aaactgctca aaattctgtg cgtgttttac agaaggccgc 1921 tataacaata ctagatggaa tttcacagta ttcactgaga ctcattgatg ctatgatgtt 1981 cacatctgat ttggctacta acaatctagt tgtaatggcc tacattacag gtggtgttgt 2041 tcagttgact tcgcagtggc taactaacat ctttggcact gtttatgaaa aactcaaacc 2101 cgtccttgat tggcttgaag agaagtttaa ggaaggtgta gagtttctta gagacggttg 2161 ggaaattgtt aaatttatct caacctgtgc ttgtgaaatt gtcggtggac aaattgtcac 2221 ctgtgcaaag gaaattaagg agagtgttca gacattcttt aagcttgtaa ataaattttt 2281 ggctttgtgt gctgactcta tcattattgg tggagctaaa cttaaagcct tgaatttagg 2341 tgaaacattt gtcacgcact caaagggatt gtacagaaag tgtgttaaat ccagagaaga 2401 aactggccta ctcatgcctc taaaagcccc aaaagaaatt atcttcttag agggagaaac 2461 acttcccaca gaagtgttaa cagaggaagt tgtcttgaaa actggtgatt tacaaccatt 2521 agaacaacct actagtgaag ctgttgaagc tccattggtt ggtacaccag tttgtattaa 2581 cgggcttatg ttgctcgaaa tcaaagacac agaaaagtac tgtgcccttg cacctaatat 2641 gatggtaaca aacaatacct tcacactcaa aggcggtgca ccaacaaagg ttacttttgg 2701 tgatgacact gtgatagaag tgcaaggtta caagagtgtg aatatcactt ttgaacttga 2761 tgaaaggatt gataaagtac ttaatgagaa gtgttctgcc tatacagttg aactcggtac 2821 agaagtaaat gagttcgcct gtgttgtggc agatgctgtc ataaaaactt tgcaaccagt 2881 atctgaatta cttacaccac tgggcattga tttagatgag tggagtatgg ctacatacta 2941 cttatttgat gagtctggtg agtttaaatt ggcttcacat atgtattgtt ctttttaccc 3001 tccagatgag gatgaagaag aaggtgattg tgaagaagaa gagtttgagc catcaactca 3061 atatgagtat ggtactgaag atgattacca aggtaaacct ttggaatttg gtgccacttc 3121 tgctgctctt caacctgaag aagagcaaga agaagattgg ttagatgatg atagtcaaca 3181 aactgttggt caacaagacg gcagtgagga caatcagaca actactattc aaacaattgt 3241 tgaggttcaa cctcaattag agatggaact tacaccagtt gttcagacta ttgaagtgaa 3301 tagttttagt ggttatttaa aacttactga caatgtatac attaaaaatg cagacattgt 3361 ggaagaagct aaaaaggtaa aaccaacagt ggttgttaat gcagccaatg tttaccttaa 3421 acatggagga ggtgttgcag gagccttaaa taaggctact aacaatgcca tgcaagttga 3481 atctgatgat tacatagcta ctaatggacc acttaaagtg ggtggtagtt gtgttttaag 3541 cggacacaat cttgctaaac actgtcttca tgttgtcggc ccaaatgtta acaaaggtga 3601 agacattcaa cttcttaaga gtgcttatga aaattttaat cagcacgaag ttctacttgc 3661 accattatta tcagctggta tttttggtgc tgaccctata cattctttaa gagtttgtgt 3721 agatactgtt cgcacaaatg tctacttagc tgtctttgat aaaaatctct atgacaaact 3781 tgtttcaagc tttttggaaa tgaagagtga aaagcaagtt gaacaaaaga tcgctgagat 3841 tcctaaagag gaagttaagc catttataac tgaaagtaaa ccttcagttg aacagagaaa 3901 acaagatgat aagaaaatca aagcttgtgt tgaagaagtt acaacaactc tggaagaaac 3961 taagttcctc acagaaaact tgttacttta tattgacatt aatggcaatc ttcatccaga 4021 ttctgccact cttgttagtg acattgacat cactttctta aagaaagatg ctccatatat 4081 agtgggtgat gttgttcaag agggtgtttt aactgctgtg gttataccta ctaaaaaggc 4141 tggtggcact actgaaatgc tagcgaaagc tttgagaaaa gtgccaacag acaattatat 4201 aaccacttac ccgggtcagg gtttaaatgg ttacactgta gaggaggcaa agacagtgct 4261 taaaaagtgt aaaagtgcct tttacattct accatctatt atctctaatg agaagcaaga 4321 aattcttgga actgtttctt ggaatttgcg agaaatgctt gcacatgcag aagaaacacg 4381 caaattaatg cctgtctgtg tggaaactaa agccatagtt tcaactatac agcgtaaata 4441 taagggtatt aaaatacaag agggtgtggt tgattatggt gctagatttt acttttacac 4501 cagtaaaaca actgtagcgt cacttatcaa cacacttaac gatctaaatg aaactcttgt 4561 tacaatgcca cttggctatg taacacatgg cttaaatttg gaagaagctg ctcggtatat 4621 gagatctctc aaagtgccag ctacagtttc tgtttcttca cctgatgctg ttacagcgta 4681 taatggttat cttacttctt cttctaaaac acctgaagaa cattttattg aaaccatctc 4741 acttgctggt tcctataaag attggtccta ttctggacaa tctacacaac taggtataga 4801 atttcttaag agaggtgata aaagtgtata ttacactagt aatcctacca cattccacct 4861 agatggtgaa gttatcacct ttgacaatct taagacactt ctttctttga gagaagtgag 4921 gactattaag gtgtttacaa cagtagacaa cattaacctc cacacgcaag ttgtggacat 4981 gtcaatgaca tatggacaac agtttggtcc aacttatttg gatggagctg atgttactaa 5041 aataaaacct cataattcac atgaaggtaa aacattttat gttttaccta atgatgacac 5101 tctacgtgtt gaggcttttg agtactacca cacaactgat cctagttttc tgggtaggta 5161 catgtcagca ttaaatcaca ctaaaaagtg gaaataccca caagttaatg gtttaacttc 5221 tattaaatgg gcagataaca actgttatct tgccactgca ttgttaacac tccaacaaat 5281 agagttgaag tttaatccac ctgctctaca agatgcttat tacagagcaa gggctggtga 5341 agctgctaac ttttgtgcac ttatcttagc ctactgtaat aagacagtag gtgagttagg 5401 tgatgttaga gaaacaatga gttacttgtt tcaacatgcc aatttagatt cttgcaaaag 5461 agtcttgaac gtggtgtgta aaacttgtgg acaacagcag acaaccctta agggtgtaga 5521 agctgttatg tacatgggca cactttctta tgaacaattt aagaaaggtg ttcagatacc 5581 ttgtacgtgt ggtaaacaag ctacaaaata tctagtacaa caggagtcac cttttgttat 5641 gatgtcagca ccacctgctc agtatgaact taagcatggt acatttactt gtgctagtga 5701 gtacactggt aattaccagt gtggtcacta taaacatata acttctaaag aaactttgta 5761 ttgcatagac ggtgctttac ttacaaagtc ctcagaatac aaaggtccta ttacggatgt 5821 tttctacaaa gaaaacagtt acacaacaac cataaaacca gttacttata aattggatgg 5881 tgttgtttgt acagaaattg accctaagtt ggacaattat tataagaaag acaattctta 5941 tttcacagag caaccaattg atcttgtacc aaaccaacca tatccaaacg caagcttcga 6001 taattttaag tttgtatgtg ataatatcaa atttgctgat gatttaaacc agttaactgg 6061 ttataagaaa cctgcttcaa gagagcttaa agttacattt ttccctgact taaatggtga 6121 tgtggtggct attgattata aacactacac accctctttt aagaaaggag ctaaattgtt 6181 acataaacct attgtttggc atgttaacaa tgcaactaat aaagccacgt ataaaccaaa 6241 tacctggtgt atacgttgtc tttggagcac aaaaccagtt gaaacatcaa attcgtttga 6301 tgtactgaag tcagaggacg cgcagggaat ggataatctt gcctgcgaag atctaaaacc 6361 agtctctgaa gaagtagtgg aaaatcctac catacagaaa gacgttcttg agtgtaatgt 6421 gaaaactacc gaagttgtag gagacattat acttaaacca gcaaataata gtttaaaaat 6481 tacagaagag gttggccaca cagatctaat ggctgcttat gtagacaatt ctagtcttac 6541 tattaagaaa cctaatgaat tatctagagt attaggtttg aaaacccttg ctactcatgg 6601 tttagctgct gttaatagtg tcccttggga tactatagct aattatgcta agccttttct 6661 taacaaagtt gttagtacaa ctactaacat agttacacgg tgtttaaacc gtgtttgtac 6721 taattatatg ccttatttct ttactttatt gctacaattg tgtactttta ctagaagtac 6781 aaattctaga attaaagcat ctatgccgac tactatagca aagaatactg ttaagagtgt 6841 cggtaaattt tgtctagagg cttcatttaa ttatttgaag tcacctaatt tttctaaact 6901 gataaatatt ataatttggt ttttactatt aagtgtttgc ctaggttctt taatctactc 6961 aaccgctgct ttaggtgttt taatgtctaa tttaggcatg ccttcttact gtactggtta 7021 cagagaaggc tatttgaact ctactaatgt cactattgca acctactgta ctggttctat 7081 accttgtagt gtttgtctta gtggtttaga ttctttagac acctatcctt ctttagaaac 7141 tatacaaatt accatttcat cttttaaatg ggatttaact gcttttggct tagttgcaga 7201 gtggtttttg gcatatattc ttttcactag gtttttctat gtacttggat tggctgcaat 7261 catgcaattg tttttcagct attttgcagt acattttatt agtaattctt ggcttatgtg 7321 gttaataatt aatcttgtac aaatggcccc gatttcagct atggttagaa tgtacatctt 7381 ctttgcatca ttttattatg tatggaaaag ttatgtgcat gttgtagacg gttgtaattc 7441 atcaacttgt atgatgtgtt acaaacgtaa tagagcaaca agagtcgaat gtacaactat 7501 tgttaatggt gttagaaggt ccttttatgt ctatgctaat ggaggtaaag gcttttgcaa 7561 actacacaat tggaattgtg ttaattgtga tacattctgt gctggtagta catttattag 7621 tgatgaagtt gcgagagact tgtcactaca gtttaaaaga ccaataaatc ctactgacca 7681 gtcttcttac atcgttgata gtgttacagt gaagaatggt tccatccatc tttactttga 7741 taaagctggt caaaagactt atgaaagaca ttctctctct cattttgtta acttagacaa 7801 cctgagagct aataacacta aaggttcatt gcctattaat gttatagttt ttgatggtaa 7861 atcaaaatgt gaagaatcat ctgcaaaatc agcgtctgtt tactacagtc agcttatgtg 7921 tcaacctata ctgttactag atcaggcatt agtgtctgat gttggtgata gtgcggaagt 7981 tgcagttaaa atgtttgatg cttacgttaa tacgttttca tcaactttta acgtaccaat 8041 ggaaaaactc aaaacactag ttgcaactgc agaagctgaa cttgcaaaga atgtgtcctt 8101 agacaatgtc ttatctactt ttatttcagc agctcggcaa gggtttgttg attcagatgt 8161 agaaactaaa gatgttgttg aatgtcttaa attgtcacat caatctgaca tagaagttac 8221 tggcgatagt tgtaataact atatgctcac ctataacaaa gttgaaaaca tgacaccccg 8281 tgaccttggt gcttgtattg actgtagtgc gcgtcatatt aatgcgcagg tagcaaaaag 8341 tcacaacatt gctttgatat ggaacgttaa agatttcatg tcattgtctg aacaactacg 8401 aaaacaaata cgtagtgctg ctaaaaagaa taacttacct tttaagttga catgtgcaac 8461 tactagacaa gttgttaatg ttgtaacaac aaagatagca cttaagggtg gtaaaattgt 8521 taataattgg ttgaagcagt taattaaagt tacacttgtg ttcctttttg ttgctgctat 8581 tttctattta ataacacctg ttcatgtcat gtctaaacat actgactttt caagtgaaat 8641 cataggatac aaggctattg atggtggtgt cactcgtgac atagcatcta cagatacttg 8701 ttttgctaac aaacatgctg attttgacac atggtttagc cagcgtggtg gtagttatac 8761 taatgacaaa gcttgcccat tgattgctgc agtcataaca agagaagtgg gttttgtcgt 8821 gcctggtttg cctggcacga tattacgcac aactaatggt gactttttgc atttcttacc 8881 tagagttttt agtgcagttg gtaacatctg ttacacacca tcaaaactta tagagtacac 8941 tgactttgca acatcagctt gtgttttggc tgctgaatgt acaattttta aagatgcttc 9001 tggtaagcca gtaccatatt gttatgatac caatgtacta gaaggttctg ttgcttatga 9061 aagtttacgc cctgacacac gttatgtgct catggatggc tctattattc aatttcctaa 9121 cacctacctt gaaggttctg ttagagtggt aacaactttt gattctgagt actgtaggca 9181 cggcacttgt gaaagatcag aagctggtgt ttgtgtatct actagtggta gatgggtact 9241 taacaatgat tattacagat ctttaccagg agttttctgt ggtgtagatg ctgtaaattt 9301 acttactaat atgtttacac cactaattca acctattggt gctttggaca tatcagcatc 9361 tatagtagct ggtggtattg tagctatcgt agtaacatgc cttgcctact attttatgag 9421 gtttagaaga gcttttggtg aatacagtca tgtagttgcc tttaatactt tactattcct 9481 tatgtcattc actgtactct gtttaacacc agtttactca ttcttacctg gtgtttattc 9541 tgttatttac ttgtacttga cattttatct tactaatgat gtttcttttt tagcacatat 9601 tcagtggatg gttatgttca cacctttagt acctttctgg ataacaattg cttatatcat 9661 ttgtatttcc acaaagcatt tctattggtt ctttagtaat tacctaaaga gacgtgtagt 9721 ctttaatggt gtttccttta gtacttttga agaagctgcg ctgtgcacct ttttgttaaa 9781 taaagaaatg tatctaaagt tgcgtagtga tgtgctatta cctcttacgc aatataatag 9841 atacttagct ctttataata agtacaagta ttttagtgga gcaatggata caactagcta 9901 cagagaagct gcttgttgtc atctcgcaaa ggctctcaat gacttcagta actcaggttc 9961 tgatgttctt taccaaccac cacaaacctc tatcacctca gctgttttgc agagtggttt 10021 tagaaaaatg gcattcccat ctggtaaagt tgagggttgt atggtacaag taacttgtgg 10081 tacaactaca cttaacggtc tttggcttga tgacgtagtt tactgtccaa gacatgtgat 10141 ctgcacctct gaagacatgc ttaaccctaa ttatgaagat ttactcattc gtaagtctaa 10201 tcataatttc ttggtacagg ctggtaatgt tcaactcagg gttattggac attctatgca 10261 aaattgtgta cttaagctta aggttgatac agccaatcct aagacaccta agtataagtt 10321 tgttcgcatt caaccaggac agactttttc agtgttagct tgttacaatg gttcaccatc 10381 tggtgtttac caatgtgcta tgaggcccaa tttcactatt aagggttcat tccttaatgg 10441 ttcatgtggt agtgttggtt ttaacataga ttatgactgt gtctcttttt gttacatgca 10501 ccatatggaa ttaccaactg gagttcatgc tggcacagac ttagaaggta acttttatgg 10561 accttttgtt gacaggcaaa cagcacaagc agctggtacg gacacaacta ttacagttaa 10621 tgttttagct tggttgtacg ctgctgttat aaatggagac aggtggtttc tcaatcgatt 10681 taccacaact cttaatgact ttaaccttgt ggctatgaag tacaattatg aacctctaac 10741 acaagaccat gttgacatac taggacctct ttctgctcaa actggaattg ccgttttaga 10801 tatgtgtgct tcattaaaag aattactgca aaatggtatg aatggacgta ccatattggg 10861 tagtgcttta ttagaagatg aatttacacc ttttgatgtt gttagacaat gctcaggtgt 10921 tactttccaa agtgcagtga aaagaacaat caagggtaca caccactggt tgttactcac 10981 aattttgact tcacttttag ttttagtcca gagtactcaa tggtctttgt tctttttttt 11041 gtatgaaaat gcctttttac cttttgctat gggtattatt gctatgtctg cttttgcaat 11101 gatgtttgtc aaacataagc atgcatttct ctgtttgttt ttgttacctt ctcttgccac 11161 tgtagcttat tttaatatgg tctatatgcc tgctagttgg gtgatgcgta ttatgacatg 11221 gttggatatg gttgatacta gtttgtctgg ttttaagcta aaagactgtg ttatgtatgc 11281 atcagctgta gtgttactaa tccttatgac agcaagaact gtgtatgatg atggtgctag 11341 gagagtgtgg acacttatga atgtcttgac actcgtttat aaagtttatt atggtaatgc 11401 tttagatcaa gccatttcca tgtgggctct tataatctct gttacttcta actactcagg 11461 tgtagttaca actgtcatgt ttttggccag aggtattgtt tttatgtgtg ttgagtattg 11521 ccctattttc ttcataactg gtaatacact tcagtgtata atgctagttt attgtttctt 11581 aggctatttt tgtacttgtt actttggcct cttttgttta ctcaaccgct actttagact 11641 gactcttggt gtttatgatt acttagtttc tacacaggag tttagatata tgaattcaca 11701 gggactactc ccacccaaga atagcataga tgccttcaaa ctcaacatta aattgttggg 11761 tgttggtggc aaaccttgta tcaaagtagc cactgtacag tctaaaatgt cagatgtaaa 11821 gtgcacatca gtagtcttac tctcagtttt gcaacaactc agagtagaat catcatctaa 11881 attgtgggct caatgtgtcc agttacacaa tgacattctc ttagctaaag atactactga 11941 agcctttgaa aaaatggttt cactactttc tgttttgctt tccatgcagg gtgctgtaga 12001 cataaacaag ctttgtgaag aaatgctgga caacagggca accttacaag ctatagcctc 12061 agagtttagt tcccttccat catatgcagc ttttgctact gctcaagaag cttatgagca 12121 ggctgttgct aatggtgatt ctgaagttgt tcttaaaaag ttgaagaagt ctttgaatgt 12181 ggctaaatct gaatttgacc gtgatgcagc catgcaacgt aagttggaaa agatggctga 12241 tcaagctatg acccaaatgt ataaacaggc tagatctgag gacaagaggg caaaagttac 12301 tagtgctatg cagacaatgc ttttcactat gcttagaaag ttggataatg atgcactcaa 12361 caacattatc aacaatgcaa gagatggttg tgttcccttg aacataatac ctcttacaac 12421 agcagccaaa ctaatggttg tcataccaga ctataacaca tataaaaata cgtgtgatgg 12481 tacaacattt acttatgcat cagcattgtg ggaaatccaa caggttgtag atgcagatag 12541 taaaattgtt caacttagtg aaattagtat ggacaattca cctaatttag catggcctct 12601 tattgtaaca gctttaaggg ccaattctgc tgtcaaatta cagaataatg agcttagtcc 12661 tgttgcacta cgacagatgt cttgtgctgc cggtactaca caaactgctt gcactgatga 12721 caatgcgtta gcttactaca acacaacaaa gggaggtagg tttgtacttg cactgttatc 12781 cgatttacag gatttgaaat gggctagatt ccctaagagt gatggaactg gtactatcta 12841 tacagaactg gaaccacctt gtaggtttgt tacagacaca cctaaaggtc ctaaagtgaa 12901 gtatttatac tttattaaag gattaaacaa cctaaataga ggtatggtac ttggtagttt 12961 agctgccaca gtacgtctac aagctggtaa tgcaacagaa gtgcctgcca attcaactgt 13021 attatctttc tgtgcttttg ctgtagatgc tgctaaagct tacaaagatt atctagctag 13081 tgggggacaa ccaatcacta attgtgttaa gatgttgtgt acacacactg gtactggtca 13141 ggcaataaca gttacaccgg aagccaatat ggatcaagaa tcctttggtg gtgcatcgtg 13201 ttgtctgtac tgccgttgcc acatagatca tccaaatcct aaaggatttt gtgacttaaa 13261 aggtaagtat gtacaaatac ctacaacttg tgctaatgac cctgtgggtt ttacacttaa 13321 aaacacagtc tgtaccgtct gcggtatgtg gaaaggttat ggctgtagtt gtgatcaact 13381 ccgcgaaccc atgcttcagt cagctgatgc acaatcgttt ttaaacgggt ttgcggtgta 13441 agtgcagccc gtcttacacc gtgcggcaca ggcactagta ctgatgtcgt atacagggct 13501 tttgacatct acaatgataa agtagctggt tttgctaaat tcctaaaaac taattgttgt 13561 cgcttccaag aaaaggacga agatgacaat ttaattgatt cttactttgt agttaagaga 13621 cacactttct ctaactacca acatgaagaa acaatttata atttacttaa ggattgtcca 13681 gctgttgcta aacatgactt ctttaagttt agaatagacg gtgacatggt accacatata 13741 tcacgtcaac gtcttactaa atacacaatg gcagacctcg tctatgcttt aaggcatttt 13801 gatgaaggta attgtgacac attaaaagaa atacttgtca catacaattg ttgtgatgat 13861 gattatttca ataaaaagga ctggtatgat tttgtagaaa acccagatat attacgcgta 13921 tacgccaact taggtgaacg tgtacgccaa gctttgttaa aaacagtaca attctgtgat 13981 gccatgcgaa atgctggtat tgttggtgta ctgacattag ataatcaaga tctcaatggt 14041 aactggtatg atttcggtga tttcatacaa accacgccag gtagtggagt tcctgttgta 14101 gattcttatt attcattgtt aatgcctata ttaaccttga ccagggcttt aactgcagag 14161 tcacatgttg acactgactt aacaaagcct tacattaagt gggatttgtt aaaatatgac 14221 ttcacggaag agaggttaaa actctttgac cgttatttta aatattggga tcagacatac 14281 cacccaaatt gtgttaactg tttggatgac agatgcattc tgcattgtgc aaactttaat 14341 gttttattct ctacagtgtt cccacttaca agttttggac cactagtgag aaaaatattt 14401 gttgatggtg ttccatttgt agtttcaact ggataccact tcagnnnnnn nnnnnnnnta 14461 cataatcagg atgtaaactt acatagctct agacttagtt ttaaggaatt acttgtgtat 14521 gctgctgacc ctgctatgca cgctgcttct ggtaatctat tactagataa acgcactacg 14581 tgcttttcag tagctgcact tactaacaat gttgcttttc aaactgtcaa acccggtaat 14641 tttaacaaag acttctatga ctttgctgtg tctaagggtt tctttaagga aggaagttct 14701 gttgaattaa aacacttctt ctttgctcag gatggtaatg ctgctatcag cgattatgac 14761 tactatcgtt ataatctacc aacaatgtgt gatatcagac aactactatt tgtagttgaa 14821 gttgttgata agtactttga ttgttacgat ggtggctgta ttaatgctaa ccaagtcatc 14881 gtcaacaacc tagacaaatc agctggtttt ccatttaata aatggggtaa ggctagactt 14941 tattatgatt caatgagtta tgaggatcaa gatgcacttt tcgcatatac aaaacgtaat 15001 gtcatcccta ctataactca aatgaatctt aagtatgcca ttagtgcaaa gaatagagct 15061 cgcaccgtag ctggtgtctc tatctgtagt actatgacca atagacagtt tcatcaaaaa 15121 ttattgaaat caatagccgc cactagagga gctactgtag taattggaac aagcaaattc 15181 tatggtggtt ggcacaacat gttaaaaact gtttatagtg atgtagaaaa ccctcacctt 15241 atgggttggg attatcctaa atgtgataga gccatgccta acatgcttag aattatggcc 15301 tcacttgttc ttgctcgcaa acatacaacg tgttgtagct tgtcacaccg tttctataga 15361 ttagctaatg agtgtgctca agtattgagt gaaatggtca tgtgtggcgg ttcactatat 15421 gttaaaccag gtggaacctc atcaggagat gccacaactg cttatgctaa tagtgttttt 15481 aacatttgtc aagctgtcac ggccaatgtt aatgcacttt tatctactga tggtaacaaa 15541 attgccgata agtatgtccg caatttacaa cacagacttt atgagtgtct ctatagaaat 15601 agagatgttg acacagactt tgtgaatgag ttttacgcat atttgcgtaa acatttctca 15661 atgatgatac tctctgacga tgctgttgtg tgtttcaata gcacttatgc atctcaaggt 15721 ctagtggcta gcataaagaa ctttaagtca gttctttatt atcaaaacaa tgtttttatg 15781 tctgaagcaa aatgttggac tgagactgac cttactaaag gacctcatga attttgctct 15841 caacatacaa tgctagttaa acagggtgat gattatgtgt accttcctta cccagatcca 15901 tcaagaatcc taggggccgg ctgttttgta gatgatatcg taaaaacaga tggtacactt 15961 atgattgaac ggttcgtgtc tttagctata gatgcttacc cacttactaa acatcctaat 16021 caggagtatg ctgatgtctt tcatttgtac ttacaataca taagaaagct acatgatgag 16081 ttaacaggac acatgttaga catgtattct gttatgctta ctaatgataa cacttcaagg 16141 tattgggaac ctgagtttta tgaggctatg tacacaccgc atacagtctt acaggctgtt 16201 ggggcttgtg ttctttgcaa ttcacagact tcattaagat gtggtgcttg catacgtaga 16261 ccattcttat gttgtaaatg ctgttacgac catgtcatat caacatcaca taaattagtc 16321 ttgtctgtta atccgtatgt ttgcaatgct ccaggttgtg atgtcacaga tgtgactcaa 16381 ctttacttag gaggtatgag ctattattgt aaatcacata aaccacccat tagttttcca 16441 ttgtgtgcta atggacaagt ttttggttta tataaaaata catgtgttgg tagcgataat 16501 gttactgact ttaatgcaat tgcaacatgt gactggacaa atgctggtga ttacatttta 16561 gctaacacct gtactgaaag actcaagctt tttgcagcag aaacgctcaa agctactgag 16621 gagacattta aactgtctta tggtattgct actgtacgtg aagtgctgtc tgacagagaa 16681 ttacatcttt catgggaagt tggtaaacct agaccaccac ttaaccgaaa ttatgtcttt 16741 actggttatc gtgtaactaa aaacagtaaa gtacaaatag gagagtacac ctttgaaaaa 16801 ggtgactatg gtgatgctgt tgtttaccga ggtacaacaa cttacaaatt aaatgttggt 16861 gattattttg tgctgacatc acatacagta atgccattaa gtgcacctac actagtgcca 16921 caagagcact atgttagaat tactggctta tacccaacac tcaatatctc agatgagttt 16981 tctagcaatg ttgcaaatta tcaaaaggtt ggtatgcaaa agtattctac actccaggga 17041 ccacctggta ctggtaagag tcattttgct attggcctag ctctctacta cccttctgct 17101 cgcatagtgt atacagcttg ctctcatgcc gctgttgatg cactatgtga gaaggcatta 17161 aaatatttgc ctatagataa atgtagtaga attatacctg cacgtgctcg tgtagagtgt 17221 tttgataaat tcaaagtgaa ttcaacatta gaacagtatg tcttttgtac tgtaaatgca 17281 ttgcctgaga cgacagcaga tatagttgtc tttgatgaaa tttcaatggc cacaaattat 17341 gatttgagtg ttgtcaatgc cagattacgt gctaagcact atgtgtacat tggcgaccct 17401 gctcaattac ctgcaccacg cacattgcta actaagggca cactagaacc agaatatttc 17461 aattcagtgt gtagacttat gaaaactata ggtccagaca tgttcctcgg aacttgtcgg 17521 cgttgtcctg ctgaaattgt tgacactgtg agtgctttgg tttatgataa taagcttaaa 17581 gcacataaag acaaatcagc tcaatgcttt aaaatgtttt ataagggtgt tatcacgcat 17641 gatgtttcat ctgcaattaa caggccacaa ataggcgtgg taagagaatt ccttacacgt 17701 aaccctgctt ggagaaaagc tgtctttatt tcaccttata attcacagaa tgctgtagcc 17761 tcaaagattt tgggactacc aactcaaact gttgattcat cacagggctc agaatatgac 17821 tatgtcatat tcactcaaac cactgaaaca gctcactctt gtaatgtaaa cagatttaat 17881 gttgctatta ccagagcaaa agtaggcata ctttgcataa tgtctgatag agacctttat 17941 gacaagttgc aatttacaag tcttgaaatt ccacgtagga atgtggcaac tttacaagct 18001 gaaaatgtaa caggactctt taaagattgt agtaaggtaa tcactgggtt acatcctaca 18061 caggcaccta cacacctcag tgttgacact aaattcaaaa ctgaaggttt atgtgttgac 18121 atacctggca tacctaagga catgacctat agaagactca tctctatgat gggttttaaa 18181 atgaattatc aagttaatgg ttaccctaac atgtttatca cccgcgaaga agctataaga 18241 catgtacgtg catggattgg cttcgatgtc gaggggtgtc atgctactag agaagctgtt 18301 ggtaccaatt tacctttaca gctaggtttt tctacaggtg ttaacctagt tgctgtacct 18361 acaggttatg ttgatacacc taataataca gatttttcca gagttagtgc taaaccaccg 18421 cctggagatc aatttaaaca cctcatacca cttatgtaca aaggacttcc ttggaatgta 18481 gtgcgtataa agattgtaca aatgttaagt gacacactta aaaatctctc tgacagagtc 18541 gtatttgtct tatgggcaca tggctttgag ttgacatcta tgaagtattt tgtgaaaata 18601 ggacctgagc gcacctgttg tctatgtgat agacgtgcca catgcttttc cactgcttca 18661 gacacttatg cctgttggca tcattctatt ggatttgatt acgtctataa tccgtttatg 18721 attgatgttc aacaatgggg ttttacaggt aacctacaaa gcaaccatga tctgtattgt 18781 caagtccatg gtaatgcaca tgtagctagt tgtgatgcaa tcatgactag gtgtttagct 18841 gtccacgagt gctttgttaa gcgtgttgac tggactattg aatatcctat aattggtgat 18901 gaactgaaga ttaatgcggc ttgtagaaag gttcaacaca tggttgttaa agctgcatta 18961 ttagcagaca aattcccagt tcttcacgac attggtaacc ctaaagctat taagtgtgta 19021 cctcaagctg atgtagaatg gaagttctat gatgcacagc cttgtagtga caaagcttat 19081 aaaatagaag aattattcta ttcttatgcc acacattctg acaaattcac agatggtgta 19141 tgcctatttt ggaattgcaa tgtcgataga tatcctgcta attccattgt ttgtagattt 19201 gacactagag tgctatctaa ccttaacttg cctggttgtg atggtggcag tttgtatgta 19261 aataaacatg cattccacac accagctttt gataaaagtg cttttgttaa tttaaaacaa 19321 ttaccatttt tctattactc tgacagtcca tgtgagtctc atggaaaaca agtagtgtca 19381 gatatagatt atgtaccact aaagtctgct acgtgtataa cacgttgcaa tttaggtggt 19441 gctgtctgta gacatcatgc taatgagtac agattgtatc tcgatgctta taacatgatg 19501 atctcagctg gctttagctt gtgggtttac aaacaatttg atacttataa cctctggaac 19561 acttttacaa gacttcagag tttagaaaat gtggctttta atgttgtaaa taagggacac 19621 tttgatggac aacagggtga agtaccagtt tctatcatta ataacactgt ttacacaaaa 19681 gttgatggtg ttgatgtaga attgtttgaa aataaaacaa cattacctgt taatgtagca 19741 tttgagcttt gggctaagcg caacattaaa ccagtaccag aggtgaaaat actcaataat 19801 ttgggtgtgg acattgctgc taatactgtg atctgggact acaaaagaga tgctccagca 19861 catatatcta ctattggtgt ttgttctatg actgacatag ccaagaaacc aactgaaacg 19921 atttgtgcac cactcactgt cttttttgat ggtagagttg atggtcaagt agacttattt 19981 agaaatgccc gtaatggtgt tcttattaca gaaggtagtg ttaaaggttt acaaccatct 20041 gtaggtccca aacaagctag tcttaatgga gtcacattaa ttggagaagc cgtaaaaaca 20101 cagttcaatt attataagaa agttgatggt gttgtccaac aattacctga aacttacttt 20161 actcagagta gaaatttaca agaatttaaa cccaggagtc aaatggaaat tgatttctta 20221 gaattagcta tggatgaatt cattgaacgg tataaattag aaggctatgc cttcgaacat 20281 atcgtttatg gagattttag tcatagtcag ttaggtggtt tacatctact gattggacta 20341 gctaaacgtt ttaaggaatc accttttgaa ttagaagatt ttattcctat ggacagtaca 20401 gttaaaaact atttcataac agatgcgcaa acaggttcat ctaagtgtgt gtgttctgtt 20461 attgatttat tacttgatga ttttgttgaa ataataaaat cccaagattt atctgtagtt 20521 tctaaggttg tcaaagtgac tattgactat acagaaattt catttatgct ttggtgtaaa 20581 gatggccatg tagaaacatt ttacccaaaa ttacaatcta gtcaagcgtg gcaaccgggt 20641 gttgctatgc ctaatcttta caaaatgcaa agaatgctat tagaaaagtg tgaccttcaa 20701 aattatggtg atagtgcaac attacctaaa ggcataatga tgaatgtcgc aaaatatact 20761 caactgtgtc aatatttaaa cacattaaca ttagctgtac cctataatat gagagttata 20821 cattttggtg ctggttctga taaaggagtt gcaccaggta cagctgtttt aagacagtgg 20881 ttgcctacgg gtacgctgct tgtcgattca gatcttaatg actttgtctc tgatgcagat 20941 tcaactttga ttggtgattg tgcaactgta catacagcta ataaatggga tctcattatt 21001 agtgatatgt acgaccctaa gactaaaaat gttacaaaag aaaatgactc taaagagggt 21061 tttttcactt acatttgtgg gtttatacaa caaaagctag ctcttggagg ttccgtggct 21121 ataaagataa cagaacattc ttggaatgct gatctttata agctcatggg acacttcgca 21181 tggtggacag cctttgttac taatgtgaat gcgtcatcat ctgaagcatt tttaattgga 21241 tgtaattatc ttggcaaacc acgcgaacaa atagatggtt atgtcatgca tgcaaattac 21301 atattttgga ggaatacaaa tccaattcag ttgtcttcct attctttatt tgacatgagt 21361 aaatttcccc ttaaattaag gggtactgct gttatgtctt taaaagaagg tcaaatcaat 21421 gatatgattt tatctcttct tagtaaaggt agacttataa ttagagaaaa caacagagtt 21481 gttatttcta gtgatgttct tgttaacaac taaacgaaca atgtttgttt ttcttgtttt 21541 attgccacta gtctctagtc agtgtgttaa tcttacaacc agaactcaat taccccctgc 21601 atacactaat tctttcacac gtggtgttta ttaccctgac aaagttttca gatcctcagt 21661 tttacattca actcaggact ttttcttacc tttcttttcc aatgttactt ggttccatgc 21721 tatacatgtc tctgggacca atggtactaa gaggtttgat aaccctgtcc taccatttaa 21781 tgatggtgtt tattttgctt ccactgagaa gtctaacata ataagaggct ggatttttgg 21841 tactacttta gattcgaaga cccagtccct acttattgtt aataacgcta ctaatgttgt 21901 tattaaagtc tgtgaatttc aattttgtaa tgatccattt ttgggtgttt attaccacaa 21961 aaacaacaaa agttggatgg aaagtgagtt cagagtttat tctagtgcga ataattgcac 22021 ttttgaatat gtctctcagc cttttcttat ggaccttgaa ggaaaacagg gtaatttcaa 22081 aaatcttagg gaatttgtgt ttaagaatat tgatggttat tttaaaatat attctaagca 22141 cacgcctatt aatttagtgc gtgatctccc tcagggtttt tcggctttag aaccattggt 22201 agatttgcca ataggtatta acatcactag gtttcaaact ttacttgctt tacatagaag 22261 ttatttgact cctggtgatt cttcttcagg ttggacagct ggtgctgcag cttattatgt 22321 gggttatctt caacctagga cttttctatt aaaatataat gaaaatggaa ccattacaga 22381 tgctgtagac tgtgcacttg atcctctctc agaaacaaag tgtactttga aatccttcac 22441 tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc caaccaacag aatctattgt 22501 tagatttcct aatattacaa acttgtgccc ttttggtgaa gtttttaacg ccaccagatt 22561 tgcatctgtt tatgcttgga acaggaagag aatcagcaac tgtgttgctg attattctgt 22621 cctatataat tccgcatcat tttccacttt taagtgttat ggagtgtctc ctactaaatt 22681 aaatgatctc tgctttacta atgtctatgc agattcattt gtaattagag gtgatgaagt 22741 cagacaaatc gctccagggc aaactggaaa gattgctgat tataattata aattaccaga 22801 tgattttaca ggctgcgtta tagcttggaa ttctaacaat cttgattcta aggttggtgg 22861 taattataat tacctgtata gattgtttag gaagtctaat ctcaaacctt ttgagagaga 22921 tatttcaact gaaatctatc aggccggtag cacaccttgt aatggtgttg aaggttttaa 22981 ttgttacttt cctttacaat catatggttt ccaacccact aatggtgttg gttaccaacc 23041 atacagagta gtagtacttt cttttgaact tctacatgca ccagcaactg tttgtggacc 23101 taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat ttcaacttca atggtttaac 23161 aggcacaggt gttcttactg agtctaacaa aaagtttctg cctttccaac aatttggcag 23221 agacattgct gacactactg atgctgtccg tgatccacag acacttgaga ttcttgacat 23281 tacaccatgt tcttttggtg gtgtcagtgt tataacacca ggaacaaata cttctaacca 23341 ggttgctgtt ctttatcagg gtgttaactg cacagaagtc cctgttgcta ttcatgcaga 23401 tcaacttact cctacttggc gtgtttattc tacaggttct aatgtttttc aaacacgtgc 23461 aggctgttta ataggggctg aacatgtcaa caactcatat gagtgtgaca tacccattgg 23521 tgcaggtata tgcgctagtt atcagactca gactaattct cctcggcggg cacgtagtgt 23581 agctagtcac tccatcattg cctacactat gtcacttggt gcagaaaatt cagttgctta 23641 ctctaataac tctattgcca tacccacaaa ttttactatt agtgttacca cagaaattct 23701 accagtgtct atgaccaaga catcagtaga ttgtacaatg tacatttgtg gtgattcaac 23761 tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt acacaattaa accgtgcttt 23821 aactggaata gctgttgaac aagacaaaaa cacccaagaa gtttttgcac aagtcaaaca 23881 aatttacaaa acaccaccaa ttaaagattt tggtggtttt aatttttcac aaatattacc 23941 agatccatca aaaccaagca agaggtcatt tattgaagat ctacttttca acaaagtgac 24001 acttgcagat gctggcttca tcaaacaata tggtgattgc cttggtgata ttgctgctag 24061 agacctcatt tgtgcacaaa agtttaacgg ccttactgtt ttgccacctt tgctcacaga 24121 tgaaatgatt gctcaataca cttctgcact gttagcgggt acaatcactt ctggttggac 24181 ctttggtgca ggtgctgcat tacaaatacc atttgctatg caaatggctt ataggtttaa 24241 tggtattgga gttacacaga atgttctcta tgagaaccaa aaattgattg ccaaccaatt 24301 taatagtgct attggcaaaa ttcaagactc actttcttcc acagcaagtg cacttggaaa 24361 acttcaagat gtggtcaacc aaaatgcaca agctttaaac acgcttgtta aacaacttag 24421 ctccaatttt ggtgcaattt caagtgtttt aaatgatatc ctttcacgtc ttgacaaagt 24481 tgaggctgaa gtgcaaattg ataggttgat cacaggcaga cttcaaagtt tgcagacata 24541 tgtgactcaa caattaatta gagctgcaga aatcagagct tctgctaatc ttgctgctac 24601 taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt gatttttgtg gaaagggcta 24661 tcatcttatg tccttccctc agtcagcacc tcatggtgta gtcttcttgc atgtgactta 24721 tgtccctgca caagaaaaga acttcacaac tgctcctgcc atttgtcatg atggaaaagc 24781 acactttcct cgtgaaggtg tctttgtttc aaatggcaca cactggtttg taacacaaag 24841 gaatttttat gaaccacaaa tcattactac agacaacaca tttgtgtctg gtaactgtga 24901 tgttgtaata ggaattgtca acaacacagt ttatgatcct ttgcaacctg aattagactc 24961 attcaaggag gagttagata aatattttaa gaatcataca tcaccagatg ttgatttagg 25021 tgacatctct ggcattaatg cttcagttgt aaacattcaa aaagaaattg accgcctcaa 25081 tgaggttgcc aagaatttaa atgaatctct catcgatctc caagaacttg gaaagtatga 25141 gcagtatata aaatggccat ggtacatttg gctaggtttt atagctggct tgattgccat 25201 agtaatggtg acaattatgc tttgctgtat gaccagttgc tgtagttgtc tcaagggctg 25261 ttgttcttgt ggatcctgct gcaaatttga tgaagacgac tctgagccag tgctcaaagg 25321 agtcaaatta cattacacat aaacgaactt atggatttgt ttatgagaat cttcacaatt 25381 ggaactgtaa ctttgaagca aggtgaaatc aaggatgcta ctccttcaga ttttgttcgc 25441 gctactgcaa cgataccgat acaagcctca ctccctttcg gatggcttat tgttggcgtt 25501 gcacttcttg ctgtttttca tagcgcttcc aaaatcataa ccctcaaaaa gagatggcaa 25561 ctagcactct ccaagggtgt tcactttgtt tgcaacttgc tgttgttgtt tgtaacagtt 25621 tactcacacc ttttgctcgt tgctgctggc cttgaagccc cttttctcta tctttatgct 25681 ttagtctact tcttgcagag tataaacttt gtaagaataa taatgaggct ttggctttgc 25741 tggaaatgcc gttccaaaaa cccattactt tatgatgcca actattttct ttgctggcat 25801 actaattgtt acgactattg tataccttac aatagtgtaa cttcttcaat tgtcattact 25861 tcaggtgatg gcacaacaag tcctatttct gaacatgact accagattgg tggttatact 25921 gaaaaatggg aatctggagt aaaagactgt gttgtattac acagttactt cacttcagac 25981 tattaccagc tgtactcaac tcaattgagt acagacactg gtgttgaaca tgttaccttc 26041 ttcatctaca ataaaattgt tgatgagcct gaagaacatg tccaaattca cacaatcgac 26101 ggttcatccg gagttgttaa tccagtaatg gaaccaattt atgatgaacc gacgacgact 26161 actagcgtgc ctttgtaagc acaagctgat gagtacgaac ttatgtactc attcgtttcg 26221 gaagagacag gtacgttaat agttaatagc gtacttcttt ttcttgcttt cgtggtattc 26281 ttgctagtta cactagccat ccttactgcg cttcgattgt gtgcgtactg ctgcaatatt 26341 gttaacgtga gtcttgtaaa accttctttt tacgtttact ctcgtgttaa aaatctgaat 26401 tcttctagag ttcctgatct tctggtctaa acgaactaaa tattatatta gtttttctgt 26461 ttggaacttt aattttagcc atggcagatt ccaacggtac tattaccgtt gaagagctta 26521 aaaagctcct tgaacaatgg aacctagtaa taggtttcct attccttaca tggatttgtc 26581 ttctacaatt tgcctatgcc aacaggaata ggtttttgta tataattaag ttaattttcc 26641 tctggctgtt atggccagta actttagctt gttttgtgct tgctgctgtt tatagaataa 26701 attggatcac cggtggaatt gctatcgcaa tggcttgtct tgtaggcttg atgtggctca 26761 gctacttcat tgcttctttc agactgtttg cgcgtacgcg ttccatgtgg tcattcaatc 26821 cagaaactaa cattcttctc aacgtgccac tccatggcac tattctgacc agaccgcttc 26881 tagaaagtga actcgtaatc ggagctgtga tccttcgtgg acatcttcgt attgctggac 26941 accatctagg acgctgtgac atcaaggacc tgcctaaaga aatcactgtt gctacatcac 27001 gaacgctttc ttattacaaa ttgggagctt cgcagcgtgt agcaggtgac tcaggttttg 27061 ctgcatacag tcgctacagg attggcaact ataaattaaa cacagaccat tccagtagca 27121 gtgacaatat tgctttgctt gtacagtaag tgacaacaga tgtttcatct cgttgacttt 27181 caggttacta tagcagagat attactaatt attatgagga cttttaaagt ttccatttgg 27241 aatcttgatt acatcataaa cctcataatt aaaaatttat ctaagtcact aactgagaat 27301 aaatattctc aattagatga agagcaacca atggagattg attaaacgaa catgaaaatt 27361 attcttttct tggcactgat aacactcgct acttgtgagc tttatcacta ccaagagtgt 27421 gttagaggta caacagtact tttaaaagaa ccttgctctt ctggaacata cgagggcaat 27481 tcaccatttc atcctctagc tgataacaaa tttgcactga cttgctttag cactcaattt 27541 gcttttgctt gtcctgacgg cgtaaaacac gtctatcagt tacgtgccag atcagtttca 27601 cctaaactgt tcatcagaca agaggaagtt caagaacttt actctccaat ttttcttatt 27661 gttgcggcaa tagtgtttat aacactttgc ttcacactca aaagaaagac agaatgattc 27721 aactttcatt aattgacttc tatttgtgct ttttagcctt tctgctattc cttgttttaa 27781 ttatgcttat tatcttttgg ttctcacttg aactgcaaga tcataatgaa acttgtcacg 27841 cctaaacgaa catgaaattt cttgttttct taggaatcat cacaactgta gctgcatttc 27901 accaagaatg tagtttacag tcatgtactc aacatcaacc atatgtagtt gatgacccgt 27961 gtcctattca cttctattct aaatggtata ttagagtagg agctagaaaa tcagcacctt 28021 taattgaatt gtgcgtggat gaggctggtt ctaaatcacc cattcagtac atcgatatcg 28081 gtaattatac agtttcctgt ttacctttta caattaattg ccaggaacct aaattgggta 28141 gtcttgtagt gcgttgttcg ttctatgaag actttttaga gtatcatgac gttcgtgttg 28201 ttttagattt catctaaacg aacaaactaa aatgtctgat aatggacccc aaaatcagcg 28261 aaatgcaccc cgcattacgt ttggtggacc ctcagattca actggcagta accagaatgg 28321 agaacgcagt ggggcgcgat caaaacaacg tcggccccaa ggtttaccca ataatactgc 28381 gtcttggttc accgctctca ctcaacatgg caaggaagac cttaaattcc ctcgaggaca 28441 aggcgttcca attaacacca atagcagtcc agatgaccaa attggctact accgaagagc 28501 taccagacga attcgtggtg gtgacggtaa aatgaaagat ctcagtccaa gatggtattt 28561 ctactaccta ggaactgggc cagaagctgg acttccctat ggtgctaaca aagacggcat 28621 catatgggtt gcaactgagg gagccttgaa tacaccaaaa gatcacattg gcacccgcaa 28681 tcctgctaac aatgctgcaa tcgtgctaca acttcctcaa ggaacaacat tgccaaaagg 28741 cttctacgca gaagggagca gaggcggcag tcaagcctct tctcgttcct catcacgtag 28801 tcgcaacagt ttaagaaatt caactccagg cagcagtagg ggaacttctc ctgctagaat 28861 ggctggcaat ggcggtgatg ctgctcttgc tttgctgctg cttgacagat tgaaccagct 28921 tgagagcaaa atgtctggta aaggccaaca acaacaaggc caaactgtca ctaagaaatc 28981 tgctgctgag gcttctaaga agcctcggca aaaacgtact gccactaaag catacaatgt 29041 aacacaagct ttcggcagac gtggtccaga acaaacccaa ggaaattttg gggaccagga 29101 actaatcaga caaggaactg attacaaaca ttggccgcaa attgcacaat ttgcccccag 29161 cgcttcagcg ttcttcggaa tgtcgcgcat tggcatggaa gtcacacctt cgggaacgtg 29221 gttgacctac acaggtgcca tcaaattgga tgacaaagat ccaaatttca aagatcaagt 29281 cattttgctg aataagcata ttgacgcata caaaacattc ccaccaacag agcctaaaaa 29341 ggacaaaaag aagaaggctg atgaaactca agccttaccg cagagacaga agaaacagca 29401 aactgtgact cttcttcctg ctgcagattt ggatgatttc tccaaacaat tgcaacaatc 29461 catgagcagt gctgactcaa ctcaggccta aactcatgca gaccacacaa ggcagatggg 29521 ctatataaac gttttcgctt ttccgtttac gatatatagt ctactcttgt gcagaatgaa 29581 ttctcgtaac tacatagcac aagtagatgt agttaacttt aatctcacat agcaatcttt 29641 aatcagtgtg taacattagg gaggacttga aagagccacc acattttcac cgaggccacg 29701 cggagtacga tcgagtgtac agtgaacaat gctagggaga gctgcctata tggaagagcc 29761 ctaatgtgta aaattaattt tagtagtgct atccccatgt //