LOCUS       DF836563               49654 bp    DNA     linear   CON 10-FEB-2015
DEFINITION  Mucor ambiguus DNA, scaffold: scf_mam1_v10274, strain NBRC 6742.
ACCESSION   DF836563 BBKB01000000
VERSION     DF836563.1
DBLINK      BioProject:PRJDB3023
            BioSample:SAMD00019078
KEYWORDS    WGS; STANDARD_DRAFT.
SOURCE      Mucor ambiguus
  ORGANISM  Mucor ambiguus
            Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota;
            Mucoromycotina; Mucoromycetes; Mucorales; Mucorineae; Mucoraceae;
            Mucor.
REFERENCE   1  (bases 1 to 49654)
  AUTHORS   Koike,H. and Takeda,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2014) to the DDBJ/EMBL/GenBank databases.
            Contact:Hideaki Koike
            National Institute of Advanced Industrial Science and Technology
            (AIST), Bioproduction Research Institute; Higashi 1 1 1, Tsukuba,
            Ibaraki 305-8566, Japan
REFERENCE   2  
  AUTHORS   Takeda,I., Yamane,N., Morita,T., Tamano,K., Machida,M., Baker,S.
            and Koike,H.
  TITLE     Draft genome sequence of an oleaginous Mucoromycotina fungus Mucor
            ambiguus NBRC6742
  JOURNAL   Unpublished (2014)
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal           :: Noncontiguous Finished
            Current Finishing Status :: Standard Draft
            Assembly Method          :: Soapdenovo v. 2.0
            Genome Coverage          :: 200x
            Sequencing Technology    :: Illumina Miseq
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..49654
                     /culture_collection="NBRC:6742"
                     /db_xref="taxon:91626"
                     /mol_type="genomic DNA"
                     /note="scaffold: scf_mam1_v10274"
                     /organism="Mucor ambiguus"
                     /strain="NBRC 6742"
     CDS             complement(<1..187)
                     /codon_start=1
                     /locus_tag="MAM1_0274d09079"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09549.1"
                     /transl_table=1
                     /translation="MALSDKQTLSKGRDPIGGKAKDSRHTSNKQKDPMQSYAQILKSD
                     QTRDTFDLTPHTSDELGE"
     CDS             complement(join(280..735,790..885))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09080"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09550.1"
                     /transl_table=1
                     /translation="MSPNTRSIKDWNTAGQLRIPTPIVWKMYVEVALLRRSTLENILE
                     FPNDCNARCXKETPATDSTNKESSSSEVPATIPLGEEDGKDATLSREEQQAIGVDVDF
                     EQPSSVVAQEQQGDTPEDDNDDDMNDDDDDEVDVTDINMADVEKEAAASSLPLQEVLQ
                     KMKRQIRIQRHKVKXIPKNDFTS"
     assembly_gap    908..6575
                     /estimated_length=5668
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     CDS             join(6697..6746,6818..7607)
                     /codon_start=1
                     /locus_tag="MAM1_0274c09081"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09551.1"
                     /transl_table=1
                     /translation="MMFTPPRHPESNGIVERNILDKLLCRDEQLKDDDLFKYFDKESS
                     DKSFIVIQGILYKLVPKPRNLLQNNKFKVVPKHLVTKVLEEFYNARLTGHGGVSKTMY
                     NLKRNHLWWHGMXQILGLWDSKLRLIRFVYNNMLNQATGYSPFEIIHGRSARTIQSAF
                     KEENKPKLYRDKAETPQSKFAAALKHDVDAIAEHVYAKLSLTEDAHVNVNALQEGDLV
                     YLFNSAISSWKKPRKLSLDWLAPMKIVKVITSTRYEIMEHDTRLQKLHRNVHISLLKK
                     HHQ"
     assembly_gap    6765..6805
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7637..9474
                     /estimated_length=1838
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11133..11183
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11390..12476
                     /estimated_length=1087
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     CDS             complement(join(12596..13278,13457..13793))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09082"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09552.1"
                     /transl_table=1
                     /translation="MQSAKELTAVSKKLAASDFFLIPTDLGDSVSIRFTEQHLLMYNG
                     VKRIIMRHVKSRIQRMRFNKKGSFKFKNGNKKATRKQHSNINAEAQPLFSETSLRKTT
                     ERILGSLDXYRIIDQLLCEVRGLYGKYSDFQNSPDGIQDVILHSNTQTSYFYQDVFQS
                     ALLDANFIDPENKFIYMEGEEICYGAMQKPFKMIQIANALLPPIVWTEGTSRQVELID
                     YNNHQDDLLPPNSFYVQAYVNDGSIRFILNKVIAVSLLNGAIQKSTFTIQEVSVKLES
                     TFESVCDTMWNHLMALDCLTDIDQHGHCDIHLTGEYSTADYHFFKCSIKRMTEELVRF
                     ILEAQ"
     assembly_gap    14043..16697
                     /estimated_length=2655
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17678..18631
                     /estimated_length=954
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     CDS             join(20850..20863,20923..21055,21117..21890)
                     /codon_start=1
                     /locus_tag="MAM1_0274c09083"
                     /note="top hit is XP_006959760.1 originated in Wallemia
                     sebi CBS 633.66"
                     /product="brix-domain-containing protein"
                     /protein_id="GAN09553.1"
                     /transl_table=1
                     /translation="MLRTVKAKNARAKRFLKNREAKVHENPKNTLVVRGSSTSQVVSD
                     ALKDLYALKRPNAIYFSKKNEIKPFEDEEKLEFFSQKNDSAHLVIGTHSKKRPHNLTF
                     VRMYNHQMLDMYEFGIENPKSMGSIPGPKCSLGLKPLMIFNGERFESDEKCKYIKNYF
                     LDFFNGEETDAINLAGLEHVISLTATPDDRILFRTYAIQMKKSGLKTPRVELEEMGPS
                     YDLKIKRTSVPKQEIWNSAVRVSKEVKPKKAKNIEVDEMGDKYGRIHLGKQELDKIQT
                     RKMKGLKRGANEKEDNDEASKKQKVDSDDE"
     CDS             complement(join(22012..22249,22322..22523,22586..22713,
                     22778..23187,23249..23329,23391..23527,23585..23717))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09084"
                     /note="top hit is XP_007862286.1 originated in
                     Gloeophyllum trabeum ATCC 11539"
                     /product="Ser/Thr phosphatase family superfamily protein"
                     /protein_id="GAN09554.1"
                     /transl_table=1
                     /translation="MLLKSTLSLSLVAAAVSSLVSAAVIIPNAHKRSEGASGKFVHVT
                     DFHLDPYYVEGASVSSLCHRKDGDNGKGVAGKFGALGSDCDSPKALIDATFEFLKQQA
                     SDADFVIYTGDSVRHDRDYQDMRGDDEVRSTQQQVVQYFRETFSSKVIPVLGNNDVET
                     HNNVMAYDDSYSNFVSTWAPFDLSLGQDFLQGGYYVQEITSNLRTINTNTMAFFKKNE
                     LLYTDCDVPGPGRTQLDWLKKSLEDTRAQNSKAYILAHVPPNSKQDKHFYKNRCYDEY
                     YSLLGSYSDVILAHFSGHFNYDQLTAVLQNKQDGTYTRVAALGEEEVPMTAAELETYK
                     VVGVLFNAPSIVPQQNPAVRIYTYDTEGTQYPYGTILDWDQYYANLEEANQSGVLQYK
                     LEYTASKLYNVNHFDASGLQGVFEAISLDETVRQQYTAFRAVDGEKAADI"
     CDS             complement(join(24330..26533,26661..26670))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09085"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09555.1"
                     /transl_table=1
                     /translation="MYAARRRKCMYLKNPDETYIRLDPVNESTSPSSSSSTHLNSINT
                     QAISESYFRDIGDQVQLKQQQQQQQHHQQQQHQHNGSNGSSSTPRDDNNKIFAQDQPD
                     NLFSSNTQSNGQSISTQPTSQPATRPDKMIEQLTDGLVRLTLHHDASKLGNENVTPWR
                     SYGEFVRWTPEPYLPSYYTAPIDMPSRPTQEHLISVFFDECHHILPTLSRSMFYDQLK
                     IKGPLVTPLLLNIIYAHAAKRINNPAFRSEQNNPSTQGDLFYNRARRLVDDFLDVPRI
                     STVIALLYMALYDCDHIPQSGTRIRSSRAWMYSGMAIRMCFELGLHTANYSSQMSQCD
                     IELRKRVLWTCFVMDKLESCTMERPWMLKSKDIALDFPTPLPEDSLQERLVLEGFNQL
                     CRLMILMEKVIHFFTYDMKNLSLWTISEENQILQYLDAIHQWRDMLPNELQWTTPANN
                     PNIQRFMPAVPSSAVVANLHLICYDLELSLIMCCRYQDEDLHRERRRTLANTITQIVS
                     LTVQHQHLMYTFAVSTFSGIFAALTHAVDFDNPRLDVSEGAKLQFRKSLEDVRLIVER
                     IPMRDLRNFARLVDLTLQPNTQMMGSSMNVPQTSYASAFSAISDLVQKDGGSSSSNSM
                     EPNGNMYTPHQEVLENGSGYGSVSPERRRSPFPNNPMISGQFAFDACYQQQASSTSSP
                     SGSVSAAAVAAAAAAVGAVGTPSAASGPTNKSIEPADYTFELISVADEWARSLIY"
     CDS             complement(join(28700..29141,29202..29989))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09086"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09556.1"
                     /transl_table=1
                     /translation="MNDFSFPSMSKDMSKVGGILYPTETSTFTRPNPINKSNSFSQPQ
                     EYDQWRSNSLPTSAPMSLSNTTIMNNTNNNTSMNSNNNNGSNPAINPVSCDLQDVIIY
                     YQSQPELLRLILLSKVEEDKRRAEEAKLRAKELDMFLLQQQQQMNGTPQTPDTSFTPP
                     ALVSSNSNMMSNSSTTTATTTAASTPTTTTNTATNNLLNANQFGQIPRRPSALEMLMD
                     DSDCNRRDSALGSSFDGSANSDELDDRTFSPHATSAATASMLSMSFTAQPMISARNSL
                     HPLSPPYTLNTTIQPLEDTFSSQIKTNTSSAEPPRRYDSYFPQRPRRRREMQAISKIV
                     ETRDYPYVDGFFWKNNGNTIQKKTGNKSVYYKCSNSSKGCPVNKTVTWKENGEYLIKY
                     RGEHLAECGKVQRIVDM"
     assembly_gap    30921..31717
                     /estimated_length=797
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    33507..34275
                     /estimated_length=769
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     CDS             join(35161..35190,35250..35458,35519..36257)
                     /codon_start=1
                     /locus_tag="MAM1_0274c09087"
                     /note="top hit is WP_012110560.1 originated in
                     Parvibaculum lavamentivorans"
                     /product="glutathione S-transferase"
                     /protein_id="GAN09557.1"
                     /transl_table=1
                     /translation="MSSTAQDVVLHWFPGSPYCQKIIWVLNYKKVDYKTALINRLEPR
                     PLRRPLDGGYRKSPILQIGNHVYCDTKSIIPALEKQFPEPSLHPKLTTNPNVSSEALA
                     RGLTIWLDSHLFTTIFSQIPIQTFTADVLKDRSDLVGYQLDSEAMTAAAPFMKAAILS
                     ELRVAESILAEKAWVLDTNTPSLVDFSLAMMVFFCKNVVGEDWVQSNLKRLHEHMNRI
                     MSVSSDIMEETRSKITELEAIDILKHHESDALPRDFEVHESVLPIELGQLVAVTPLDT
                     GKVPAIGKLMRSTIDETVISHKNAEFNTTSITHFPTIGFIVVPAKPQAN"
     CDS             join(37114..37359,37432..37812,37884..37892)
                     /codon_start=1
                     /locus_tag="MAM1_0274c09088"
                     /note="top hit is XP_006677706.1 originated in
                     Batrachochytrium dendrobatidis JAM81"
                     /product="conserved hypothetical protein"
                     /protein_id="GAN09558.1"
                     /transl_table=1
                     /translation="MVLKHNNQLPNQHFRKQWDLRVKTWFDQAGRKKSRRIARVQKAG
                     RIAPRPIDGLLRPAVRCPTVRYNMKLRSGRGFTLEELKEAGVRRKEARSIGIAVDFRR
                     RNKSNESLELNVQRLKAYKAKLIVFPRKAGKAKKGDSEAAEVASAVQLRGPLFPVEQV
                     AAAPEARAITAEEKSTNAYMKLRYARSAARTLGAREKRARDKAEEEANKKK"
     CDS             complement(join(38097..38473,38531..38708,38767..38790,
                     38847..39384,39442..39557,39612..39642,39702..39896,
                     39951..40041,40098..40118,40191..40209,40266..40323,
                     40382..40455))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09089"
                     /note="top hit is XP_007379022.1 originated in
                     Punctularia strigosozonata HHB-11173 SS5"
                     /product="gamma-glutamyltranspeptidase"
                     /protein_id="GAN09559.1"
                     /transl_table=1
                     /translation="MTASSLPFISRRSTVYGTHAIVSSSQPLATQAGIDILKKGGNAA
                     DAAVAVAAALNVTEPGSTGIGGDAFCLFYDAKSKTVKGLNGSGRTPAALTLEHLRNEG
                     ISGASLPTTTPHAVTVPGAAAAWVDTVKNFGSGKIDLTTILTPAIDLAENGFPVSHIS
                     ANSWNEEVKLLIETNPGQVDWLIDNKRAPTEGEIMSVPTLAQTFRALGAEGKEGFYGG
                     RIGQAIVDALRSRGGVMTLEDLAQHNSELIEPISLDYHDWTVWEIPPNGQGITTLIAL
                     GIIEALEESGRINFNEIKHNSADYVHIVTEALRLAFADTRHYVADPQVEHVPTEALLS
                     KEYLRERAKLVDLEHRNDNVCKGYPEKTGNTVYFSVVDEEGNACSFINSTFMHFGSHI
                     IADNTGIVLHNRGCNFVLIEDHPNCLGPSKRPYHTIIPSMITRKTDNGHELEACFGVM
                     GAFMQPQGQVQVILNMKHYQSNPQHALDLPRLCVAPPKGNPLLSGGAYTFTEVTQSVI
                     YLEDGLQDTASELEAKGHTCYVLHNHARSMFGRGQIIRSVTDKRTGKRVLAAGSDPRG
                     DGCATGW"
     CDS             complement(join(40781..42141,42201..42376,42445..42648,
                     42710..42717))
                     /codon_start=1
                     /locus_tag="MAM1_0274d09090"
                     /note="no homologous protein detected"
                     /product="hypothetical protein"
                     /protein_id="GAN09560.1"
                     /transl_table=1
                     /translation="MGGVTIAHQPNNEVVNDDSYSPNFPVDVKIDASTESLNDEQFNQ
                     LFATSAEWSNNLPVEDLTGEVQELMMRESMSSWLQPWGGENAACLSDFDTQMYAQSIQ
                     LENLFGQNQIDFTQDMNQTAFYDELAFAPDDQSLNLSASTSSASSPSDSSTQISPIVQ
                     SVPDVHIEQLEHQDIIVEKLQVLEKDDVQVNIEDEENDNNELVMEKVEEEQIISSNKM
                     LKRRRSSSSSSDSSDDSSSGSSSEEESDSEDENVIPTTVANMSSRGRYASSSDSESES
                     ETTTGRRSRAVSPIANAYVYMHKRQIEETLLDRITNSLDPEKLPGILPILSPESNQQE
                     DEVEIDLSCLAREQLVQVLSYVDACIMEQNGGPAVNVERYINKKPKGPRTRPALVDKK
                     VQRRRKPSKPKTKSRAELTEDIDAGSSSDDEDDQERMDYSMSKPKQISEGPISMASLS
                     KKNNTVKSNRKKPVAKKNKNRRNDDDALVSSSIHVVQDPDSIAISRPKRRAAVHKRRL
                     LEEMLAPSDDEDNEDDEDSSPLVVFGEEKMDLGVIDNCTIMHQVPVIEAEQIVTLSSV
                     VEDDNEDTDEEIDIMC"
     assembly_gap    44942..45689
                     /estimated_length=748
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    46514..49484
                     /estimated_length=2971
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
CONTIG      join(BBKB01006489.1:1..907,gap(5668),BBKB01006490.1:1..189,gap(41),
            BBKB01006491.1:1..831,gap(1838),BBKB01006492.1:1..1658,gap(51),
            BBKB01006493.1:1..206,gap(1087),BBKB01006494.1:1..1566,gap(2655),
            BBKB01006495.1:1..980,gap(954),BBKB01006496.1:1..12289,gap(797),
            BBKB01006497.1:1..1789,gap(769),BBKB01006498.1:1..10666,gap(748),
            BBKB01006499.1:1..824,gap(2971),BBKB01006500.1:1..170)
//