LOCUS X92587 2514 bp DNA linear BCT 18-APR-2005 DEFINITION E.coli orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes. ACCESSION X92587 VERSION X92587.1 KEYWORDS Holliday junction-specific endonuclease. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Mahdi A.A., Sharples G.J., Mandal T.N., Lloyd R.G. TITLE Holliday junction resolvases encoded by homologous rusA genes in Escherichia coli K-12 and phage 82 JOURNAL J. Mol. Biol. 257(3), 561-573(1996). PUBMED 8648624 REFERENCE 2 (bases 1 to 2514) AUTHORS Sharples G.J. JOURNAL Submitted (27-OCT-1995) to the INSDC. G. J. Sharples, University of Nottingham, Department of Genetics, Queens Medical Centre, Nottingham,, NG7 2UH, UK FEATURES Location/Qualifiers source 1..2514 /organism="Escherichia coli" /map="12.5 minutes" /strain="K-12" /mol_type="genomic DNA" /clone_lib="Kohara lambda 159" /clone="pTM109" /db_xref="taxon:562" regulatory 50..55 /note="putative" /regulatory_class="minus_35_signal" regulatory 73..78 /regulatory_class="minus_10_signal" repeat_region 82..110 /rpt_type=INVERTED regulatory 122..127 /note="putative" /regulatory_class="ribosome_binding_site" CDS 134..235 /transl_table=11 /note="orf33" /db_xref="UniProtKB/Swiss-Prot:Q47268" /protein_id="CAA63317.1" /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA" regulatory 221..225 /regulatory_class="ribosome_binding_site" CDS 232..687 /transl_table=11 /note="orf151" /db_xref="GOA:Q47269" /db_xref="InterPro:IPR036619" /db_xref="UniProtKB/Swiss-Prot:Q47269" /protein_id="CAA63318.1" /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA" regulatory 674..679 /regulatory_class="ribosome_binding_site" CDS 687..857 /transl_table=11 /note="orf56" /note="related to nin60 (ninE) of bacteriophage lambda" /db_xref="UniProtKB/Swiss-Prot:Q47270" /protein_id="CAA63319.1" /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK KWLRLAARRSHG" regulatory 840..845 /regulatory_class="ribosome_binding_site" CDS 850..1140 /transl_table=11 /note="orf96" /db_xref="GOA:P68661" /db_xref="InterPro:IPR010774" /db_xref="PDB:3G27" /db_xref="UniProtKB/Swiss-Prot:P68661" /protein_id="CAA63320.1" /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA" regulatory 1121..1126 /regulatory_class="ribosome_binding_site" CDS 1137..1499 /transl_table=11 /gene="rus" /function="Holliday junction specific endonuclease" /note="Improved expression of rus suppresses the DNA repair defects of ruv mutants" /db_xref="GOA:P0AG74" /db_xref="InterPro:IPR008822" /db_xref="InterPro:IPR016281" /db_xref="InterPro:IPR036614" /db_xref="PDB:1Q8R" /db_xref="PDB:2H8C" /db_xref="PDB:2H8E" /db_xref="UniProtKB/Swiss-Prot:P0AG74" /protein_id="CAA63321.1" /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM PVTKGGRLELTITEMGNE" regulatory 1485..1490 /regulatory_class="ribosome_binding_site" CDS 1496..1636 /transl_table=11 /note="orf45" /db_xref="UniProtKB/Swiss-Prot:Q47272" /protein_id="CAA63322.1" /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA GV" regulatory 1621..1625 /regulatory_class="ribosome_binding_site" CDS 1633..1659 /transl_table=11 /note="orf8" /db_xref="UniProtKB/TrEMBL:Q47273" /protein_id="CAA63323.1" /translation="MKSEITIN" regulatory 1711..1722 /regulatory_class="ribosome_binding_site" CDS 1722..2105 /transl_table=11 /function="probable Q antiterminator" /note="orf127" /note="similar to phage 21 Q gene" /db_xref="GOA:Q47274" /db_xref="InterPro:IPR010534" /db_xref="UniProtKB/Swiss-Prot:Q47274" /protein_id="CAA63324.1" /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA EGIIEGMLMALDIRLEMDIVVNNSN" regulatory 2133..2156 /note="putative termination in other strand" /inference="non-experimental evidence, no additional details recorded" /regulatory_class="terminator" regulatory 2247..2284 /note="putative terminator for the rus operon" /inference="non-experimental evidence, no additional details recorded" /regulatory_class="terminator" CDS complement(2295..>2356) /codon_start=3 /transl_table=11 /gene="nmpC" /function="outer membrane protein" /db_xref="GOA:P21420" /db_xref="InterPro:IPR001702" /db_xref="InterPro:IPR001897" /db_xref="InterPro:IPR013793" /db_xref="InterPro:IPR023614" /db_xref="InterPro:IPR033900" /db_xref="UniProtKB/Swiss-Prot:P21420" /protein_id="CAA63325.1" /translation="KALGVSTDDIVAVGLVYQF" repeat_region 2357..2514 /note="insertion sequence IS5" /note="interrupts nmpC gene" BASE COUNT 675 a 555 c 635 g 649 t ORIGIN 1 aagcttccag agagtaaaag tgttttaaga aggagcaatt ctatcgattt tgattttggg 61 aaatcaacac ggcataatta tgtcaccgga gcctgaacaa ctccggtgac ttctgcgcta 121 aacggggacg tttatgcgca catacaatcc aaactctctt ctcccttcac agatgcagaa 181 atgcacctgc aattctttgc atctagcgtt tgacctctgc ggaggggaag cgtgaacctc 241 tcacaagacg gcatcaaatt acatcgcggc aacttcaccg ctatcggtcg gcagatccag 301 ccttatctgg aggagggcaa atgctttcgc atggtgctta aaccgtggcg tgagaaacgc 361 agtctttccc agaatgcact cagccacatg tggtacagcg aaatcagtga atacctcatc 421 agcaggggta aaacgttcgc cactccagct tgggtaaaag atgctctcaa acacacatat 481 ctcggttatg aaaccaaaga cctggttgat gtcgtaaccg gtgatatcac cactatccag 541 tcgttacgcc atacctccga tcttgatacc ggagagatgt atgtcttcct gtgtaaggtt 601 gaagcctggg cgatgaatat tggttgccac ctgactattc cacagagctg cgagttccag 661 ctgctgcgcg acaagcagga ggcgtaatgg ctacaccgct tattcgtgtc atgaacggac 721 acatctacag agtaccaaat cgtcgtaagc gtaaacctga gctgaagcca tccgaaatac 781 caacactgct cggatatacc gccagcttgg ttgataaaaa atggttgcga ctggcagcaa 841 ggaggagtca tggctgattt gagaaaagca gcgcgtggtc gggaatgcca ggtaagaatc 901 cctggcgtat gtaatggcaa ccctgaaacg tctgtactgg cacatatccg gctgactgga 961 ttgtgcggca ccggtacgaa accgccagac ctgattgcca ccattgcatg ttctgcctgc 1021 cacgacgaaa tcgaccgccg cacgcatttt gttgacgctg gatatgcaaa agaatgcgcg 1081 ctggaaggta tggcgagaac acaggttatc tggctgaaag agggggttat taaggcgtga 1141 atacctacag catcacatta ccctggcctc cgagcaataa tcgctattac cgccataatc 1201 gcgggcgcac gcacgtcagc gcagaggggc aggcataccg cgataacgtc gcccgaatca 1261 ttaaaaacgc aatgctggat atcggcctgg ctatgcctgt gaaaatccgc attgagtgcc 1321 acatgccgga tcgccgtcgc cgtgacctgg ataatctgca aaaagccgct tttgacgcac 1381 tcactaaagc aggtttctgg ctggatgatg ctcaggtcgt tgattaccgc gttgtgaaga 1441 tgcctgttac caaaggtggg aggctggaac tgaccatcac cgaaatgggg aatgaatgat 1501 gtttgagttt aatatggcag aacttcttcg ccaccgctgg gggcgtctgc gcttatatcg 1561 tttccccggt tctgttttga ccgattaccg aatactgaag aattacgcca aaaccctgac 1621 aggagcagga gtatgaagtc agagataaca atcaactaat actgttttgt tgatttttgc 1681 ttgtaattgg cgttctggtc tgatttttgt ggagtaagtt gatgcgtgat attcagatgg 1741 ttcttgagcg ttggggagcg tgggcggcta ataatcatga agatgtgacc tggtcgtcca 1801 ttgccgccgg ttttaaggga ttaattactt caaaagtaaa atctcgcccg caatgttgtg 1861 acgatgacgc gatgatcatt tgcgggtgca tggcccgtct gaaaaagaac aacagcgatt 1921 tgcacgattt attagtagat tattatgtag tcggtatgac attcatgtca ctggcaggta 1981 agcattgctg ctctgatggt tatatcggga aaaggttaca gaaggctgag ggcataattg 2041 aagggatgtt aatggcatta gatatccggt tagagatgga tatcgttgtt aataactcta 2101 attaatatgc caattgttta ctaaaaatta ttaaaaatgg ggcgttgaga cgcccccaaa 2161 aataaagggt aatatataac agaaggttta tatagttaga agcaaggttg tgcttctaaa 2221 ggaagtggct tgagggagcc acttatatgt tggggaggca acgcctcccg caacatatct 2281 ttttcgtaat cagattagaa ctggtaaacc agacctacag caacgatgtc atcagtgctt 2341 acaccgagtg ctttagggaa ggtgcgaata agcggggaaa ttcttctcgg ctgactcagt 2401 catttcattt cttcatgttt gagccgattt tttctcccgt aaatgccttg aatcagccta 2461 tttagaccgt ttcttcgcga tttaaggcgt tatccccagt ttttagtgag atct //