LOCUS       X81638                  2126 bp    DNA     linear   BCT 18-APR-2005
DEFINITION  Bacillus sp. plasmid pXH13 Bsp6I restriction endonuclease and
            methyltransferase genes.
ACCESSION   X81638
VERSION     X81638.1
KEYWORDS    bsp6IM gene; bsp6IR gene; methyltransferase; restriction
            endonuclease; site-specific desoxyribonuclease.
SOURCE      Bacillus sp. (in: Bacteria)
  ORGANISM  Bacillus sp. (in: Bacteria)
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Lubys A.
  JOURNAL   Submitted (14-SEP-1994) to the INSDC. A. Lubys, Institute of
            Biotechnology Fermentas, Graiciuno 8, Vilnius 2028, LITHUANIA
REFERENCE   2
  AUTHORS   Lubys A., Janulaitis A.
  TITLE     Cloning and analysis of the plasmid-borne genes encoding the Bsp6I
            restriction and modification enzymes
  JOURNAL   Gene 157(1-2), 25-29(1995).
   PUBMED   7607501
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Bacillus sp. (in: Bacteria)"
                     /plasmid="pXH13"
                     /strain="RFL6"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1409"
     regulatory      467..470
                     /gene="bsp6IR"
                     /regulatory_class="ribosome_binding_site"
     CDS             488..1012
                     /transl_table=11
                     /gene="bsp6IR"
                     /product="type ii site-specific deoxyribonuclease"
                     /EC_number="3.1.21.4"
                     /db_xref="GOA:P43419"
                     /db_xref="InterPro:IPR019037"
                     /db_xref="UniProtKB/Swiss-Prot:P43419"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="CAA57292.1"
                     /translation="MALHDFVKIDKSRFEDAVKAYFLWKELNALIKNSHNRGINFPET
                     ISETLLCAAMGFELNRGTGGDAREPKTQAIIESKATSNWDRDTTSFSPSENFDALYFL
                     RLNQRDDELYIYNTGINSDDLKSIQVNKTQTLGDQQAQGRRPRFSVIKFIIEANDIEP
                     VAKVELRTKKIIKL"
     regulatory      1102..1105
                     /gene="bsp6IM"
                     /regulatory_class="ribosome_binding_site"
     CDS             1112..2059
                     /transl_table=11
                     /gene="bsp6IM"
                     /product="site-specific DNA-methyltransferase
                     (cytosine-specific)"
                     /EC_number="2.1.1.73"
                     /db_xref="GOA:P43420"
                     /db_xref="InterPro:IPR001525"
                     /db_xref="InterPro:IPR018117"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="InterPro:IPR031303"
                     /db_xref="UniProtKB/Swiss-Prot:P43420"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="CAA57293.1"
                     /translation="MLQIASLFAGVGGIDLGFEQTGYFETVWANEYDKNAAITYQSNF
                     KNKLIIDDIRNIKVEDVPDFDVLLSGFPCTSFSVAGYRKGFEDEKSGDLFFETLRLIV
                     AKKPQVIFLENVKNLVGHDNGNTFKVIYEALESNGYHIKYQVLNAKDFGNIPQNRERI
                     YIVGFRNIEHYKNFNFPMPQPLTLTIKDMINLSDKLDDRFYYTEDKCSFYSPLQEQMT
                     SDETIYQWRRKYVRENKSNVCPTLTANMGTGGHNVPLVKTKHGIRKLTPRECFNFQGY
                     PEDFILPELAPTHLYKQAGNSVVVPVIRRIAENIYKSML"
BASE COUNT          760 a          317 c          395 g          654 t
ORIGIN      
        1 gatcttcggg ggatgcccct tcggcgggcg caaacaaacg gggtttgttt gaatcaatag
       61 gctttgggtg cagatgaatt agtcaactcc ttaatgctca cttcgttccg cttaccgttg
      121 actaataatt tattcttctt cttctcttct ttaattcttg ttatagttcc gctatcgttc
      181 cgcattcgtt tctttaaagg ttctttatcc gttcctcagc aatgcaaaga ggtgttaaat
      241 ttggtcgccc taccatcgat gtgccagaca attggaatga actctatgag caatggaaag
      301 ctaaggagat aacagcagtg aagtttataa ccgaagttag tatgaccaaa gcaacgtttt
      361 accgtaaagt taaagaatat gaaaaaagtg agcagatgga tacccccata taaatgaata
      421 agatgattat atttaatgat taagttaata tattactaga tacactagga agcacacata
      481 aacaaacatg gcactacatg attttgtaaa aatagataag tctagatttg aagatgcggt
      541 aaaggcatat ttcctttgga aagagttaaa tgctcttatc aaaaacagtc acaatagagg
      601 aatcaatttc cctgaaacta tttcagagac acttttatgt gcagcaatgg gatttgaatt
      661 aaatagagga accggtggcg acgctaggga gcctaaaacc caagcaatca ttgaatcaaa
      721 agcaacatca aattgggata gagatacaac ttctttttct ccaagtgaaa actttgatgc
      781 attatatttt ttaagattga atcagcgaga tgatgaactt tatatttaca atactggaat
      841 taattccgat gatttgaaaa gtattcaagt aaacaaaacg caaacacttg gagatcaaca
      901 ggcacaaggt cgtcgtccaa gattcagcgt tattaaattc atcattgaag cgaatgacat
      961 cgaacccgta gcaaaagttg aattacgaac gaagaaaatt atcaagttat aaaaagcaaa
     1021 ggaggtagtg tgcttccttt gcttttaaat tgcggcgcgc cgcaatttat gctataattt
     1081 gataatcatg taaaggaata gaggataaaa gatgttacaa atagcaagtt tattcgcagg
     1141 tgtaggtgga attgatttag gatttgaaca aactggatat tttgaaacgg tttgggcaaa
     1201 tgaatatgat aaaaatgcag ctattaccta tcaatcaaat tttaaaaaca aattaattat
     1261 agatgatatt cgaaatatca aagtagaaga tgttcctgat tttgatgttt tattatcagg
     1321 atttccttgt acttctttta gcgtggctgg atatagaaaa ggctttgaag atgaaaaaag
     1381 cggagattta ttttttgaaa ctttacgctt gattgtagct aaaaaaccac aagttatatt
     1441 tttagaaaat gttaaaaatc ttgtgggtca tgacaacgga aataccttta aagtcattta
     1501 tgaagcgtta gaaagcaacg gatatcatat aaaataccaa gttctaaacg caaaagattt
     1561 tgggaatata cctcaaaata gagagcgtat ctatattgta ggttttagga atattgaaca
     1621 ctataaaaat tttaattttc cgatgccaca accacttaca ttaacaataa aagatatgat
     1681 aaatctttca gataaactag atgatagatt ttattataca gaggataaat gttcttttta
     1741 ttcaccttta caagaacaaa tgacttcaga tgaaacaata tatcaatggc gtagaaaata
     1801 tgtgagagaa aacaaaagta atgtatgccc tacgttaact gcgaacatgg gaactggagg
     1861 acataatgtt ccattagtta aaacaaaaca tggaattaga aagttgactc ctagagagtg
     1921 tttcaatttt caaggttatc ctgaagattt cattttaccc gaattggctc ctacgcatct
     1981 ttataagcaa gctggaaatt ctgttgttgt tcctgtcata agacgcatag cagaaaatat
     2041 ttataaatct atgttgtaat taaatggttt tatatactgg cggtgaatat tttgaaaaat
     2101 ttaatcaagc attagatgaa gcgatc
//