LOCUS       X56066                   671 bp    mRNA    linear   ROD 14-NOV-2006
DEFINITION  Mouse mRNA for dihydrofolate reductase variant.
ACCESSION   X56066
VERSION     X56066.1
KEYWORDS    DHFR gene; dihydrofolate reductase.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Simonsen C.C.
  JOURNAL   Submitted (11-OCT-1990) to the INSDC. Simonsen C.C., Genelabs INC,
            Dept of Recombinant expression, 505 Penobscot DR, Redwood City, CA
            94063, USA.
REFERENCE   2
  AUTHORS   McIvor R.S., Simonsen C.C.
  TITLE     Isolation and characterization of a variant dihydrofolate reductase
            cDNA from methotrexate-resistant murine L5178Y cells
  JOURNAL   Nucleic Acids Res. 18(23), 7025-7032(1990).
   PUBMED   2263462
COMMENT     Data kindly reviewed (15-JAN-1991) by Simonsen C.C.
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /cell_type="lymphoma"
                     /db_xref="taxon:10090"
     5'UTR           7..22
     CDS             23..586
                     /gene="DHFR"
                     /product="dihydrofolate reductase"
                     /note="variant"
                     /db_xref="GOA:P00375"
                     /db_xref="InterPro:IPR001796"
                     /db_xref="InterPro:IPR017925"
                     /db_xref="InterPro:IPR024072"
                     /db_xref="MGI:MGI:94890"
                     /db_xref="PDB:1U70"
                     /db_xref="PDB:2FZJ"
                     /db_xref="PDB:3D80"
                     /db_xref="PDB:3D84"
                     /db_xref="PDB:3K45"
                     /db_xref="PDB:3K47"
                     /db_xref="UniProtKB/Swiss-Prot:P00375"
                     /protein_id="CAA39544.1"
                     /translation="MVRPLNCIVAVSQDMGIGKNGDLPWPPLRNEWKYFQRMTTTSSV
                     EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIE
                     QPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLL
                     PEYPGVLSEVQEEKGIKYKFEVYEKKD"
     mat_peptide     23..583
                     /gene="DHFR"
                     /product="dihydrofolate reductase"
                     /note="variant"
     variation       117..118
                     /phenotype="wildtype"
                     /gene="DHFR"
                     /replace="tc"
                     /product="dihydrofolate reductase"
                     /citation=[2]
     3'UTR           584..671
BASE COUNT          203 a          145 c          154 g          169 t
ORIGIN      
        1 aagctttatc cccgctgcca tcatggttcg accattgaac tgcatcgtcg ccgtgtccca
       61 agatatgggg attggcaaga acggagacct accctggcct ccgctcagga acgagtggaa
      121 gtacttccaa agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat
      181 gggtaggaaa acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa
      241 tatagttctc agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag
      301 tttggatgat gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt
      361 ttggatagtc ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccacctcag
      421 actctttgtg acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga
      481 tttggggaaa tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga
      541 aaaaggcatc aagtataagt ttgaagtcta cgagaagaaa gactaacagg aagatgcttt
      601 caagttctct gctcccctcc taaagctatg catttttata agaccatggg acttttgctg
      661 gctttagatc t
//