LOCUS       X16985                  1536 bp    DNA     linear   BCT 21-JUN-1994
DEFINITION  Micrococcus varians plasmid borne mvaIM gene for DNA-methylase
            (MvaI).
ACCESSION   X16985
VERSION     X16985.1
KEYWORDS    DNA methylase; restriction modification enzyme.
SOURCE      Micrococcus luteus
  ORGANISM  Micrococcus luteus
            Bacteria; Actinobacteria; Micrococcales; Micrococcaceae;
            Micrococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Klimasauskas S.
  JOURNAL   Submitted (20-OCT-1989) to the INSDC. Klimasauskas S., Institute of
            Applied Enzymology, Fermenty 8, Vilnius, 232028, Lithuanie, USSR.
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Klimasauskas S., Timinskas A., Menkevicius S., Butkiene D.,
            Butkus V., Janulaitis A.
  TITLE     Sequence motifs characteristic of
            DNA[cytosine-N4]methyltransferases: similarity to adenine and
            cytosine-C5 DNA-methylases
  JOURNAL   Nucleic Acids Res. 17(23), 9823-9832(1989).
   PUBMED   2690010
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Micrococcus luteus"
                     /plasmid
                     /strain="RFL19"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1270"
     regulatory      64..69
                     /note="-35 region"
                     /regulatory_class="promoter"
     regulatory      87..92
                     /note="-10 region"
                     /regulatory_class="promoter"
     regulatory      140..144
                     /note="pot.ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             151..1515
                     /transl_table=11
                     /note="MvaI DNA methylase (AA 1-454)"
                     /db_xref="GOA:P14244"
                     /db_xref="InterPro:IPR012327"
                     /db_xref="InterPro:IPR017985"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="UniProtKB/Swiss-Prot:P14244"
                     /protein_id="CAA34854.1"
                     /translation="MEYLNDKDQHLIDKLSKKINDNNQYLGFLNTNTKELTHRYHIYP
                     AMMIPQLAKEFIELTQQVKPEIKKLYDPFMGSGTSLVEGLAHGLEVYGTDINPLSQMM
                     SKAKTTPIEPSKLSRAISDLEYSIREMTILYHEGNYKISNLPDFDRIDFWFKEEVIIS
                     LQLIKNCINEFIEDDLKTFFMAAFSETVRHVSNTRNNEFKLYRMAPEKLEIWNPNVTE
                     EFLKRVYRNELGNMDFYRQLENVGNYSPKTIINKQSNIKLPEEFKDEMFDIVVTSPPY
                     GDSKTTVAYGQFSRLSAQWLDLKIDDETKINQLDNVMLGGKTDKNIIVNDVLEYLNSP
                     TSKSVFNLISHKDEKRALEVLQFYVDLDKSIKETTRVMKPESYQFWVVANRTVKMISI
                     PTDIIISELFKKYNVHHLYSFYRKIPNKRMPSKNSPTNKIGNHSVTMTSEIILMLKNY
                     INKS"
BASE COUNT          608 a          199 c          236 g          493 t
ORIGIN      
        1 actaattcag caattaattg atcaaatttt tgacttctcg tcataactat tcctccttgt
       61 tttttgatat gatagggcat atactatata ttactgaata ataaaaaggt ttttgtgtac
      121 taatagtgca caaaaaccaa aggagacaaa atggaatatt taaatgataa agatcaacat
      181 ttaattgata aattatcaaa aaagattaat gataataatc aatatcttgg tttcttaaat
      241 acaaatacta aagaattaac tcatagatat catatttatc ctgctatgat gatccctcaa
      301 ttggctaaag aattcattga attaactcaa caagtaaaac cagaaatcaa aaaattatat
      361 gatcctttta tgggctctgg tacttcttta gtagaaggac ttgcacatgg gttggaagta
      421 tatggaacag atataaatcc tctatcacaa atgatgagta aagctaaaac tactcctata
      481 gaaccttcaa agttgtcgag agctatttca gatcttgaat attctataag agaaatgaca
      541 attctgtatc atgaggggaa ttataaaata agcaaccttc ctgattttga tagaatagat
      601 ttttggttta aagaagaagt tataataagt ctacagttaa taaaaaattg cataaatgag
      661 tttatagaag atgatttgaa aacgttcttc atggcagcat ttagtgaaac agttaggcat
      721 gtttcaaata ctcgtaataa tgaatttaaa ctgtatagaa tggcacctga aaaattagaa
      781 atatggaatc cgaatgtaac tgaagaattt ttaaagagag tatacagaaa tgaattaggc
      841 aatatggatt tctatagaca acttgaaaat gtaggaaatt actcgcctaa aactataata
      901 aataagcaaa gcaacataaa acttccagag gaatttaaag atgaaatgtt cgatattgta
      961 gttacttctc caccatatgg tgatagtaaa acaactgtag cctatgggca attttcaaga
     1021 ttgtccgctc aatggttgga tctgaaaata gatgatgaga ctaaaataaa tcaattagat
     1081 aatgtgatgc ttggtggaaa aacagataaa aatattattg ttaatgatgt gttagaatat
     1141 ctcaattctc caacgtcgaa atcagtattt aatttaataa gtcataaaga tgaaaaaaga
     1201 gcactagaag ttcttcaatt ttatgttgat ttggataaat ctattaaaga aactacaaga
     1261 gtgatgaagc ccgagtcata tcaattttgg gtagtagcta atagaacagt aaaaatgatc
     1321 agtataccaa ctgatattat aatttctgag ttatttaaaa agtataatgt tcatcattta
     1381 tatagtttct ataggaaaat ccctaataaa cgtatgcctt caaaaaattc tcctactaat
     1441 aaaataggta atcattctgt taccatgact tctgagatta tattaatgct aaaaaattac
     1501 attaataaaa gctgatcttc ttcaatcatg cttaca
//