LOCUS       X15432                  1491 bp    DNA     linear   BCT 31-MAR-1995
DEFINITION  E. coli murF gene for UDP-MurNAc-pentapeptide sytnthetase (EC
            6.3.2.15).
ACCESSION   X15432
VERSION     X15432.1
KEYWORDS    ATP-binding protein; murF gene; UDP-N-acetylmuramyl-pentapeptide
            synthetase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Van Heijenoort J.
  JOURNAL   Submitted (29-MAY-1989) to the INSDC. Van Heijenoort J., CNRS URA
            1131 - Bat. 432, Universite Paris Sud, Unite Associee 1131 du
            Centre National de la Recherche Scientifique, Biochimie Moleculaire
            et Cellulaire, 91405 Orsay, France.
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Parquet C., Fluoret B., Menguin-Lecreulx D., Van Heijenoort J.
  TITLE     Nucleotide sequence of the murF gene encoding the
            UDP-MurNAc-pentapeptide synthetase of Escherichia coli
  JOURNAL   Nucleic Acids Res. 17(13), 5379-5379(1989).
   PUBMED   2668880
COMMENT     Data kindly reviewed (21-SEP-1989) by Van Heijennoort J.
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /map="2 min."
                     /strain="K12"
                     /mol_type="genomic DNA"
                     /clone="pLC 26-6."
                     /db_xref="taxon:562"
     regulatory      28..35
                     /note="pot. ribosome binding site"
                     /regulatory_class="ribosome_binding_site"
     CDS             40..1398
                     /transl_table=11
                     /product="UDP-MurNAC-pentapeptide presynthetase (AA -20 to
                     432)"
                     /db_xref="GOA:P11880"
                     /db_xref="InterPro:IPR000713"
                     /db_xref="InterPro:IPR004101"
                     /db_xref="InterPro:IPR005863"
                     /db_xref="InterPro:IPR013221"
                     /db_xref="InterPro:IPR035911"
                     /db_xref="InterPro:IPR036565"
                     /db_xref="InterPro:IPR036615"
                     /db_xref="PDB:1GG4"
                     /db_xref="UniProtKB/Swiss-Prot:P11880"
                     /protein_id="CAA33473.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGAAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPERALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     sig_peptide     40..99
                     /note="signal peptide (AA -20 to -1)"
     mat_peptide     100..1395
                     /note="UDP-MurNAc-pentapeptide synthetase (AA 1-432)"
     misc_feature    107..119
                     /note="ATP-binding domain A"
     misc_feature    352..360
                     /note="ATP-binding domain B"
BASE COUNT          333 a          397 c          427 g          334 t
ORIGIN      
        1 cgatcgcgtc acggtggcgc gtctgctggg ggtgattgca tgattagcgt aacccttagc
       61 caacttaccg acattctcaa cggtgaactg caaggtgcag atatcaccct tgatgctgta
      121 accactgata cccgaaaact gacgccgggc tgcctgtttg ttgccctgaa aggcgaacgt
      181 tttgatgccc acgattttgc cgaccaggcg aaagctggcg cggcaggcgc actactggtt
      241 agccgtccgc tggacatcga cctgccgcag ttaatcgtca aggatacgcg tctggcgttt
      301 ggtgaactgg ctgcatgggt tcgccagcaa gttccggcgc gcgtggttgc tctgacgggg
      361 tcctccggca aaacctccgt taaagagatg acggcggcga ttttaagcca gtgcggcaac
      421 acgctttata cggcaggcaa tctcaacaac gacatcggtg taccgatgac gctgttgcgc
      481 ttaacgccgg aatacgatta cgcagttatt gaacttggcg cgaaccatca gggcgaaata
      541 gcctggactg tgagtctgac tcgcccggaa cgtgcgctgg tcaacaacct ggcagcggcg
      601 catctggaag gttttggctc gcttgcgggt gtcgcgaaag cgaaaggtga aatctttagc
      661 ggcctgccgg aaaacggtat cgccattatg aacgccgaca acaacgactg gctgaactgg
      721 cagagcgtaa ttggctcacg caaagtgtgg cgtttctcac ccaatgccgc caacagcgat
      781 ttcaccgcca ccaatatcca tgtgacctcg cacggtacgg aatttaccct acaaacccca
      841 accggtagcg tcgatgttct gctgccgttg ccggggcgtc acaatattgc gaatgcgctg
      901 gcagccgctg cgctctccat gtccgtgggc gcaacgcttg atgctatcaa agcggggctg
      961 gcaaatctga aagctgttcc aggccgtctg ttccccatcc aactggcaga aaaccagttg
     1021 ctgctcgacg actcctacaa cgccaatgtc ggttcaatga ctgcagcagt ccaggtactg
     1081 gctgaaatgc cgggctaccg cgtgctggtg gtgggcgata tggcggaact gggcgctgaa
     1141 agcgaagcct gccatgtaca ggtgggcgag gcggcaaaag ctgctggtat tgaccgcgtg
     1201 ttaagcgtgg gtaaacaaag ccatgctatc agcaccgcca gcggcgttgg cgaacatttt
     1261 gctgataaaa ctgcgttaat tacgcgtctt aaattactga ttgctgagca acaggtaatt
     1321 acgattttag ttaagggttc acgtagtgcc gccatggaag aggtagtacg cgctttacag
     1381 gagaatggga catgttagtt tggctggccg aacatttggt caaatatatc cggctttaac
     1441 gtcttttcct atctgacgtt tcgcgccact gtcagcctgc tgcacgcgct g
//