LOCUS       X07568                  2062 bp    mRNA    linear   PLN 27-MAR-1995
DEFINITION  Spinach mRNA for nitrite reductase NiR (E.C.1.7.7.1.).
ACCESSION   X07568
VERSION     X07568.1
KEYWORDS    nitrite reductase; transit peptide.
SOURCE      Spinacia oleracea (spinach)
  ORGANISM  Spinacia oleracea
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; Caryophyllales; Chenopodiaceae; Chenopodioideae;
            Anserineae; Spinacia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Back E., Burkhart W., Moyer M., Privalle L., Rothstein S.
  TITLE     Isolation of cDNA clones coding for spinach nitrite reductase:
            complete sequence and nitrate induction
  JOURNAL   Mol. Gen. Genet. 212(1), 20-26(1988).
   PUBMED   3163766
COMMENT     Data kindly reviewed (02-SEP-1988) by ROTHSTEIN S.
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Spinacia oleracea"
                     /mol_type="mRNA"
                     /clone_lib="lambda gt11"
                     /clone="pCIB400, pCIB401"
                     /db_xref="taxon:3562"
     CDS             61..1845
                     /note="NiR precursor (AA -32 to 562)"
                     /db_xref="GOA:P05314"
                     /db_xref="InterPro:IPR005117"
                     /db_xref="InterPro:IPR006066"
                     /db_xref="InterPro:IPR006067"
                     /db_xref="InterPro:IPR036136"
                     /db_xref="PDB:2AKJ"
                     /db_xref="UniProtKB/Swiss-Prot:P05314"
                     /protein_id="CAA30453.1"
                     /translation="MASLPVNKIIPSSTTLLSSSNNNRRRNNSSIRCQKAVSPAAETA
                     AVSPSVDAARLEPRVEERDGFWVLKEEFRSGINPAEKVKIEKDPMKLFIEDGISDLAT
                     LSMEEVDKSKHNKDDIDVRLKWLGLFHRRKHHYGRFMMRLKLPNGVTTSEQTRYLASV
                     IKKYGKDGCADVTTRQNWQIRGVVLPDVPEIIKGLESVGLTSLQSGMDNVRNPVGNPL
                     AGIDPHEIVDTRPFTNLISQFVTANSRGNLSITNLPRKWNPCVIGSHDLYEHPHINDL
                     AYMPATKNGKFGFNLLVGGFFSIKRCEEAIPLDAWVSAEDVVPVCKAMLEAFRDLGFR
                     GNRQKCRMMWLIDELGMEAFRGEVEKRMPEQVLERASSEELVQKDWERREYLGVHPQK
                     QQGLSFVGLHIPVGRLQADEMEELARIADVYGSGELRLTVEQNIIIPNVENSKIDSLL
                     NEPLLKERYSPEPPILMKGLVACTGSQFCGQAIIETKARALKVTEEVQRLVSVTRPVR
                     MHWTGCPNSCGQVQVADIGFMGCMTRDENGKPCEGADVFVGGRIGSDSHLGDIYKKAV
                     PCKDLVPVVAEILINQFGAVPREREEAE"
     transit_peptide 61..156
                     /note="transit peptide (AA -32 to -1)"
     mat_peptide     157..1842
                     /note="mature NiR (AA 1 to 562)"
BASE COUNT          603 a          376 c          541 g          542 t
ORIGIN      
        1 catcatcttc atcttcatct tcatcattca tagttgcaag aaacagagca accaaaaaaa
       61 atggcatcac ttccagtcaa caagatcata ccatcatcaa cgacattact gtcatcgtcg
      121 aacaacaaca gaagaagaaa taactcatca attcgatgcc agaaggcggt ttcacccgcg
      181 gcagaaacgg ctgcagtgtc gccgtctgtg gacgcggcga ggctggagcc gagagtggag
      241 gagagagatg ggttttgggt attgaaggag gaatttagga gtgggattaa cccagctgag
      301 aaagttaaga ttgagaaaga cccaatgaag ttgtttattg aggatgggat tagtgatctt
      361 gctactttgt caatggagga agttgataaa tctaagcata ataaggatga tattgatgtt
      421 agactcaagt ggcttggact tttccatcgc cgtaaacatc actatgggag attcatgatg
      481 aggttgaagc tgccgaatgg ggtaacaacg agtgagcaga cacggtacct agcaagcgtg
      541 atcaagaagt acggaaaaga tggatgtgcg gatgtaacaa caaggcaaaa ctggcaaatt
      601 agaggagttg ttctgcctga tgtgccagag atcatcaaag ggctggaatc cgttggtctt
      661 accagcttac agagtgggat ggacaatgta aggaaccctg taggtaaccc tcttgcaggg
      721 attgaccctc atgaaattgt tgacacccga ccttttacca acctaatttc ccaatttgtc
      781 actgccaatt cgcgtggaaa cctttctatt accaatctgc caaggaagtg gaatccatgt
      841 gttattgggt cccatgatct ttatgagcat ccacacatca atgaccttgc ttacatgcct
      901 gctacaaaga atgggaaatt cgggtttaat ttgttggttg gaggattctt tagcatcaaa
      961 agatgtgaag aggcaatccc actagacgct tgggtctcag cagaagatgt ggttcctgta
     1021 tgcaaagcta tgcttgaagc tttcagggac cttggcttta gaggaaacag gcagaagtgc
     1081 agaatgatgt ggcttattga tgagcttggt atggaagcat tcaggggaga ggttgagaag
     1141 agaatgcctg agcaagttct agaaagagca tcctcagaag agctggttca gaaggactgg
     1201 gagagaagag aatacttagg agttcaccct cagaaacaac aaggacttag ctttgtgggt
     1261 ctccacattc ctgtgggccg tctgcaagct gatgagatgg aagagttagc ccgtatagct
     1321 gatgtgtatg gatcagggga gctccgtctg acagtagagc agaacataat catcccaaat
     1381 gttgaaaact caaagataga ttcactacta aacgagcctc tgttaaaaga gcgttactcc
     1441 cctgaaccac ccatcttgat gaaggggctt gtggcctgta cggggagcca attttgtgga
     1501 caagccatta tcgagaccaa ggctagggca ctcaaggtga cagaagaggt acaacgacta
     1561 gtgtctgtaa cacggcctgt taggatgcat tggaccgggt gtcctaatag ttgtggtcaa
     1621 gtacaagtgg ctgatattgg gttcatgggt tgcatgacta gggatgagaa cggtaagcct
     1681 tgtgaaggag ctgatgtgtt tgtaggagga cgtataggaa gtgactcgca tctaggagac
     1741 atttacaaga aggcagtccc atgtaaagat ttggtgcctg ttgttgctga gatattgatc
     1801 aaccaattcg gtgctgttcc tagggagagg gaagaggcag agtagtagct agactgtttt
     1861 gggtgcctgt tcttgttaac tgttatcggt attcggtaat tacttgtaat atttgcattt
     1921 tttttcaagc atataattaa attgcataaa gatcccttgt atgtctgcat aacaagatac
     1981 tcagttatgt aatgtcaata gcaggtttac tttgtttatt caataggcac tgtgaaaggg
     2041 aaagttcatt attcatttct ca
//