LOCUS       X03341                  1588 bp    DNA     linear   BCT 23-SEP-1999
DEFINITION  Bacillus lichenformis NCIB 6816 of target region encoding
            subtilisin Carlsberg.
ACCESSION   X03341
VERSION     X03341.1
KEYWORDS    serine protease; subtilisin; subtilisin Carlsberg.
SOURCE      Bacillus licheniformis
  ORGANISM  Bacillus licheniformis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Jacobs M., Eliasson M., Uhlen M., Flock J.I.
  TITLE     Cloning, sequencing and expression of subtilisin Carlsberg from
            Bacillus licheniformis
  JOURNAL   Nucleic Acids Res. 13(24), 8913-8926(1985).
   PUBMED   3001653
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Jacobs M.
  TITLE     Expression of the subtilisin Carlsberg-encoding gene in Bacillus
            licheniformis and Bacillus subtilis
  JOURNAL   Gene 152(1), 69-74(1995).
   PUBMED   7828931
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bacillus licheniformis"
                     /strain="NCIB 6816"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1402"
     misc_feature    108..153
                     /phenotype="allows subC stimulation in degU(Hy) and
                     degQ(Hy) hosts"
                     /standard_name="target region"
                     /product="subtilisin Carlsberg"
                     /function="target for action of DegU and DegQ"
                     /number=1
                     /citation=[1]
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    158..168
                     /phenotype="allows regulation by DegU and DegQ"
                     /pseudo
                     /standard_name="target region"
                     /product="subtilisin Carlsberg"
                     /function="target region for DegU and DegQ"
                     /number=2
                     /note="this region is homologous to a region indispensible
                     for regulation by DegU and DegQ of the aprE gene of
                     Bacillus subtilis "
                     /citation=[1]
     regulatory      251..256
                     /citation=[1]
                     /regulatory_class="minus_10_signal"
     mRNA            262..1531
                     /gene="subC"
                     /citation=[1]
                     /experiment="experimental evidence, no additional details
                     recorded"
     CDS             335..1474
                     /transl_table=11
                     /gene="subC"
                     /db_xref="GOA:P00780"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR010259"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR022398"
                     /db_xref="InterPro:IPR023827"
                     /db_xref="InterPro:IPR023828"
                     /db_xref="InterPro:IPR034202"
                     /db_xref="InterPro:IPR036852"
                     /db_xref="InterPro:IPR037045"
                     /db_xref="PDB:1AF4"
                     /db_xref="PDB:1AV7"
                     /db_xref="PDB:1AVT"
                     /db_xref="PDB:1BE6"
                     /db_xref="PDB:1BE8"
                     /db_xref="PDB:1BFK"
                     /db_xref="PDB:1BFU"
                     /db_xref="PDB:1C3L"
                     /db_xref="PDB:1CSE"
                     /db_xref="PDB:1OYV"
                     /db_xref="PDB:1R0R"
                     /db_xref="PDB:1SBC"
                     /db_xref="PDB:1SCA"
                     /db_xref="PDB:1SCB"
                     /db_xref="PDB:1SCD"
                     /db_xref="PDB:1SCN"
                     /db_xref="PDB:1SEL"
                     /db_xref="PDB:1VSB"
                     /db_xref="PDB:1YU6"
                     /db_xref="PDB:2SEC"
                     /db_xref="PDB:2WUV"
                     /db_xref="PDB:2WUW"
                     /db_xref="PDB:3UNX"
                     /db_xref="PDB:3VSB"
                     /db_xref="PDB:4C3U"
                     /db_xref="PDB:4C3V"
                     /db_xref="UniProtKB/Swiss-Prot:P00780"
                     /protein_id="CAB56500.1"
                     /translation="MMRKKSFWLGMLTAFMLVFTMAFSDSASAAQPAKNVEKDYIVGF
                     KSGVKTASVKKDIIKESGGKVDKQFRIINAAKAKLDKEALKEVKNDPDVAYVEEDHVA
                     HALAQTVPYGIPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEA
                     YNTDGNGHGTHVAGTVAALDNTTGVLGVAPSVSLYAVKVLNSSGSGTYSGIVSGIEWA
                     TTNGMDVINMSLGGPSGSTAMKQAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYD
                     SVIAVGAVDSNSNRASFSSVGAELEVMAPGAGVYSTYPTSTYATLNGTSMASPHVAGA
                     AALILSKHPNLSASQVRNRLSSTATYLGSSFYYGKGLINVEAAAQ"
     regulatory      1271..1305
                     /regulatory_class="terminator"
BASE COUNT          471 a          342 c          376 g          399 t
ORIGIN      
        1 gatcgacaag accgcaacct ccttcgacaa aaaatgatct cttaaaataa atgaatagta
       61 ttttcataaa atgaatcaga tggagcaatc tcctgtcatt cgcggccctc gggacctctt
      121 tccctgccag gctgaagcgg tctattcata ctttcgaact gaacattttt ctaaaacagt
      181 tattaataac caaaaaattt taaattggtc ctccaaaaaa ataggcctac catataattc
      241 attttttttc tataataaat taacagaata attggaatag attatattat ccttctattt
      301 aaattattct gaataaagag gaggagagtg agtaatgatg aggaaaaaga gtttttggct
      361 tgggatgctg acggccttca tgctcgtgtt cacgatggca ttcagcgatt ccgcttctgc
      421 tgctcaaccg gcgaaaaatg ttgaaaagga ttatattgtc ggatttaagt caggagtgaa
      481 aaccgcatct gtcaaaaagg acatcatcaa agagagcggc ggaaaagtgg acaagcagtt
      541 tagaatcatc aacgcggcaa aagcgaagct agacaaagaa gcgcttaagg aagtcaaaaa
      601 tgatccggat gtcgcttatg tggaagagga tcatgtggcc catgccttgg cgcaaaccgt
      661 tccttacggc attcctctca ttaaagcgga caaagtgcag gctcaaggct ttaagggagc
      721 gaatgtaaaa gtagccgtcc tggatacagg aatccaagct tctcatccgg acttgaacgt
      781 agtcggcgga gcaagctttg tggctggcga agcttataac accgacggca acggacacgg
      841 cacacatgtt gccggtacag tagctgcgct tgacaataca acgggtgtat taggcgttgc
      901 gccaagcgta tccttgtacg cggttaaagt actgaattca agcggaagcg gaacttacag
      961 cggcattgta agcggaatcg agtgggcgac gacaaacggc atggatgtta tcaacatgag
     1021 tcttggagga ccatcaggct caacagcgat gaaacaggcg gttgacaatg catatgcaag
     1081 aggggttgtc gttgtggcgg ctgctgggaa cagcggatct tcaggaaaca cgaatacaat
     1141 cggctatcct gcgaaatacg actctgtcat cgcagttggc gcggtagact ctaacagcaa
     1201 cagagcttca ttttccagcg tcggagcaga gcttgaagtc atggctcctg gcgcaggcgt
     1261 gtacagcact tacccaacca gcacttatgc aacattgaac ggaacgtcaa tggcttctcc
     1321 tcatgtagcg ggagcagcag ctttgatctt gtcaaaacat ccgaaccttt cagcttcaca
     1381 agtccgcaac cgtctctcca gtacggcgac ttatttggga agctccttct actatggaaa
     1441 aggtctgatc aatgtcgaag ctgccgctca ataacatatt ctaacaaatg gcatatagaa
     1501 aaagctagtg tttttagcac tagctttttc ttcattcagt tgaagactgt tcaatatttt
     1561 gaatccgttc cattatggtc ggatggcc
//