LOCUS BSU78099 6678 bp DNA linear BCT 03-JUN-1998 DEFINITION Burkholderia sp. PS12 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase (tecF), TecA1 (tecA1), TecA2 (tecA2), TecA3 (tecA3), TecA4 (tecA4), and cis-chlorobenzene dihydrodiol dehydrogenase (tecB) genes, complete cds; and itecE pseudogene, complete sequence. ACCESSION U78099 VERSION U78099.1 KEYWORDS . SOURCE Burkholderia sp. ORGANISM Burkholderia sp. Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia. REFERENCE 1 (bases 1 to 6678) AUTHORS Beil,S., Happe,B., Timmis,K.N. and Pieper,D.H. TITLE Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12--dechlorination of 1,2,4,5-tetrachlorobenzene JOURNAL Eur. J. Biochem. 247 (1), 190-199 (1997) PUBMED 9249026 REFERENCE 2 (bases 1 to 6678) AUTHORS Beil,S. TITLE Direct Submission JOURNAL Submitted (14-NOV-1996) Microbiology, GBF-National Center for Biotechnology Ltd., Mascheroder Weg 1, Braunschweig D-38124, Germany REFERENCE 3 (bases 1 to 6678) AUTHORS Beil,S. TITLE Direct Submission JOURNAL Submitted (03-JUN-1998) Microbiology, GBF-National Center for Biotechnology Ltd., Mascheroder Weg 1, Braunschweig D-38124, Germany REMARK Sequence update by submitter COMMENT On Jun 3, 1998 this sequence version replaced gi:2264416. FEATURES Location/Qualifiers source 1..6678 /organism="Burkholderia sp." /mol_type="genomic DNA" /strain="PS12" /db_xref="taxon:36773" gene 443..1273 /gene="tecF" CDS 443..1273 /gene="tecF" /note="TecF" /codon_start=1 /transl_table=11 /product="2-hydroxy-6-oxo-2,4-heptadienoate hydrolase" /protein_id="AAC46394.1" /translation="MTNANAEVGRMVRAGSIDTNLHDVGAGKPVLLVHGSGPGVTAWA NWRTVMPELSRRRRVIAPDMVGFGFTERPQGIRYGLDTWVEHLVGILDAMELDRVDFV GNSFGGGLSLAFAIRFPHRVRRLVLMGSAGVSFKLTDGLDAVWGYEPSVPNMRKVMDY FAYDRSLVSDELAELRYGASIRPGFQEAFASMFPAPRQRWVDALASTDQDIRAIRHET LILHGRDDRVVPLETSLRLNQLIEPSQLHVFGRCGHWVQIEQNQGFIRLVNDFLDTED " gene 1359..2708 /gene="tecA1" CDS 1359..2708 /gene="tecA1" /note="terminal dioxygenase large alpha-subunit" /codon_start=1 /transl_table=11 /product="TecA1" /protein_id="AAC46390.1" /translation="MNHTDTSPIKLRKNWNAREMQALFDERAGRTDPRIYTDEDLYQI ELERVFGRSWLLLGHETQIKKPGDYTTNYMGEDPVLVVRQKDGSIAVFLNQCRHRGMR ICRSDAGNAKAFTCSYHGWAYDTAGNLVNVPFEAESFPCLDKKEWSPLKARVATYKGL IFANWDHDAPDLDTYLGEAKFYMDHMLDRTEAGTEAIPGVQKWVIPCNWKLAAEQFCW DAYHAATTAHLSGILAGLPDGVELADLAPPTVGKQYRAPWGGHGSGFFIGEPDLLLAI MGPKITSYWTEGPASEKAAQRLGSVERGSKLTVEHMTVFPTCSFLLGANTVRTWHPRG PNEVEVWAFTVVDADAPDDIKEEFRRQTVRTFSAGGVFEQDDGENWVEIQHVLRGHKA RSRPFNAEMSMGQTIDDDPVYPGRISNVYSDEAARGFYAQWLRMMTSSDWAALNATR" gene 2818..3381 /gene="tecA2" CDS 2818..3381 /gene="tecA2" /note="terminal dioxygenase small beta-subunit" /codon_start=1 /transl_table=11 /product="TecA2" /protein_id="AAC46391.1" /translation="MLDSVKRADVFLRKPAPVAPELQHEIEQFYYWEAKLLNDRRFEE WFALLAADIHYFMPIRTTRIMRDARLEYSGTGEHAHFDDDAAMMKGRLRKVTSDVGWS ENPASRTRHLVSNVMIADGPVEGEYEISSAFIVYRNRLERQLDIFAGERRDTLRRNKT ETGFEIVNRTILIDQSTILANNLSFFF" gene 3390..3713 /gene="tecA3" CDS 3390..3713 /gene="tecA3" /function="ferredoxin" /codon_start=1 /transl_table=11 /product="TecA3" /protein_id="AAC46392.1" /translation="MAWTYIMRQSDLPPGEMQRHEGGPEPVMVCNVDGEFFAVQDTCT HGNWALSDGYLDGGVVECTLHFGKFCVRTGKVKALPACKPIKVFPIKVEGGDVHVDLD AGEVK" gene 3713..4945 /gene="tecA4" CDS 3713..4945 /gene="tecA4" /function="reductase" /codon_start=1 /transl_table=11 /product="TecA4" /protein_id="AAC46393.1" /translation="MATHVAIIGNGVAGFTTAQALRAEGFEGRISLIGNEPHLPYDRP SLSKAVLGGSLEHPPVLAEADWYGEARIDMLSGRSVTNLNVDARTISLDDGSTFAADA IVIATGSRARTLALPGSQLTGVVTLRTNDDVRPLCRGWTPATRLVIAGGGLIGCEVAT TARKLGLAVTILESADELLVRVLGRRIGAWLRGLLTELGVRVELGTGVAGFSGDDRLE EVLASDGRRFAADNALVCIGAEPEDQLARQAGLSCDRGVIVDDHGATHAEGVFAVGDA ASWPLRDGGRRSLETYMNAQRQAAAVAAAILGKHGSAPQVPVSWTEIAGHRMQMAGDI EGPGEFVLRGTLGDGAALLFRLRDGRIQAVVAVDAPRDFAMAARLVEARAAIEPARLA DFSNSMRDLVRAQQGDSA" gene 4942..5769 /gene="tecB" CDS 4942..5769 /gene="tecB" /note="TecB" /codon_start=1 /transl_table=11 /product="cis-chlorobenzene dihydrodiol dehydrogenase" /protein_id="AAC46395.1" /translation="MKLKGEVALVTGGGAGLGRAIVDRYVAEGARVAVLDKSAAGLEE IRKRHGDAVVGIEGDVRSLDSHREAVARCVETFGKLDCLIGNAGVWDYQTQLADIPDN GISEAFDEMFAIIVKGYILAAKAALPALYKSKGSAIFTVSNAGFYPGGGGVLYTAGKH AVIGLVKQLAHEWGPRIRVNGIAPGGILGSDIRGLKTLGLQDQTIATMPLADMLGPVL PTGRVATAEEYAGAYVFFATRADTVPLTGSVLNIDGGMGVRGLFEASLGAQLDKHFA" gene 5782..6658 /gene="itecE" /pseudo CDS 5782..6658 /gene="itecE" /note="inactive catechol 2,3-dioxygenase" /pseudo /codon_start=1 /transl_table=11 BASE COUNT 1301 a 2051 c 2135 g 1191 t ORIGIN 1 agatctggtt tggcatcttc cagcgaaaaa cgctcaacaa cgcgagcttc caaagcaccg 61 agcacctcgt cgccgccatc cacgcgttca ctgccgcgta caacgagaac gccgcacctt 121 ttgtctggcg caaacgagaa gtacggggca ctcagcttcg aaatactatt gtcaatttac 181 ggaattaaac actaggcgtg aagtggaggg tcgtcatagg gttatccctg tagctgaaaa 241 ttcgagcatg catgcaaaat cgtctcattg aaagaataac gttatttatt gaaagtgacg 301 acatgctgat tgaaaaaaga cgcttacgta tggaggcaat agtgccctgc ggcgctcatt 361 catgcaacca cattgcggcg atcggaggca gcgcgctgcc tccggccgac tgctgggtcg 421 caccgcacct ggagtcccgc agatgacgaa tgcaaacgca gaagtcggtc gcatggtgcg 481 agccggcagc atcgacacca atcttcatga cgtcggcgcc ggcaagccgg tcctgctcgt 541 tcatggttca ggccctggcg tgaccgcctg ggcgaactgg agaacggtca tgcccgagtt 601 gtcccggcgc cggcgcgtca tcgcaccgga catggtgggc ttcggcttca cggagcggcc 661 gcaagggatt cgctatggcc tcgatacgtg ggtcgagcat ctggtcggga ttcttgacgc 721 catggaactc gaccgtgtcg atttcgtggg gaattcgttc ggaggcggct tgtctctggc 781 ctttgccatc cggtttccgc accgggtccg ccggctggtg ctgatgggat cagcgggcgt 841 gagcttcaag ctcaccgatg ggctggacgc cgtgtggggc tatgagccgt ccgtgcccaa 901 catgcgcaag gtcatggact actttgccta cgaccgaagc ctcgtttccg atgagctggc 961 ggaactgcgc tacggcgcga gcatcaggcc cggcttccag gaggccttcg cgtccatgtt 1021 ccccgccccg cggcagcgat gggtggatgc gctggccagc accgatcagg acatccgggc 1081 gatccggcat gaaacgctga tcttgcatgg ccgtgacgac cgcgtggttc cccttgaaac 1141 gtcgttgcgc ctgaaccagc tgatcgagcc ctcgcagttg catgtctttg gaagatgtgg 1201 ccattgggtg cagatcgagc aaaaccaagg cttcatccgt ttggtcaacg acttcctcga 1261 cacggaggac tgatctcaag accgaagcgc tcattgattt cgaaaccgcg caatcaccca 1321 ccacccaccc atccaccacc atgaaacgtg aggagacaat gaatcacacc gacacctccc 1381 ctatcaagct gcgcaagaac tggaacgccc gcgagatgca ggcgctcttt gacgagcgcg 1441 ccggacgtac cgacccgcgt atctataccg atgaagacct gtaccagatc gaactggagc 1501 gcgtcttcgg caggtcgtgg cttctgttgg ggcatgaaac ccagatcaag aaaccgggcg 1561 actacaccac gaactatatg ggcgaagacc ccgtcttggt cgtgaggcag aaggacggca 1621 gcatcgctgt attcctgaac caatgccgcc accgcggcat gcgcatctgc cgctcggatg 1681 ccggcaacgc gaaggctttc acgtgcagct accacggctg ggcctacgac acggcgggca 1741 acctggtcaa cgtccccttc gaggctgaat ccttcccttg cctggacaag aaggaatgga 1801 gccccttgaa ggcccgggtc gcgacctaca agggcctgat cttcgccaac tgggaccacg 1861 acgccccgga tctggacacc tatctgggcg aggcgaagtt ctacatggac cacatgctcg 1921 accgtaccga ggccggcacc gaagccatcc cgggtgtgca gaagtgggtc atcccctgca 1981 actggaaact cgccgccgaa cagttctgct gggacgcgta ccacgccgcc acgaccgcgc 2041 atctgtccgg catcctggcc ggtctgccgg acggcgttga gctggccgat ctcgctccgc 2101 cgacggtcgg caagcaatac cgtgcgccgt ggggcggaca cggaagcggt ttctttattg 2161 gagaacccga tctcctgctc gccatcatgg ggccgaagat caccagctac tggaccgaag 2221 gccccgcctc tgaaaaggcg gcccagcgcc tgggcagcgt cgaacgcggc tccaaactca 2281 cggtcgagca catgactgtg tttcccacgt gctccttcct tctaggcgcc aacacggttc 2341 ggacatggca tccacgcggg ccgaacgagg tggaggtgtg ggcgttcacg gtggtcgacg 2401 ccgatgctcc cgacgacatc aaggaagagt tccgccgcca gacggtgcgc accttctccg 2461 ctggcggggt gttcgagcag gacgacgggg agaactgggt cgagatccag cacgtcctgc 2521 gcggccacaa ggcacgcagc cgaccgttca acgcggagat gagcatgggc cagaccattg 2581 acgacgatcc ggtctacccc gggcgcatca gcaacgtcta cagcgacgaa gcggcccgcg 2641 ggttttatgc ccagtggctg cggatgatga cctcgtctga ctgggccgcg ctgaacgcca 2701 cacgctgaat cacgacacct cacgccgcaa agtcgcgctg gccaggagcc gcgtccgatt 2761 tcgggccgag gccgatttag cgacccttct tcgatttgaa attatcaagg aatgaccatg 2821 cttgattccg tcaagagagc cgacgtcttt ctccgtaaac ccgcgccggt ggcgcccgag 2881 ctgcaacacg aaatcgagca gttctactac tgggaagcca agctgctcaa cgatcgccgc 2941 tttgaggagt ggttcgccct gctggcggct gacatccact acttcatgcc gatccgcacc 3001 acgcggatca tgcgggacgc acgcctggag tactcgggca ccggcgagca cgcgcacttc 3061 gacgacgatg ccgcgatgat gaaaggccgg ctgcgcaagg tcacatccga cgtgggctgg 3121 tcggagaacc ccgcttcgcg cacccggcac ctcgtgagca acgtgatgat cgccgacgga 3181 ccggtagaag gcgagtacga gatttccagc gctttcatcg tgtatcgcaa tcgcctggaa 3241 cggcaactcg acatctttgc cggcgaacgt cgcgacacgt tgcgccgcaa caagaccgag 3301 accgggttcg agattgtcaa tcggaccatc ctgatcgacc agagcaccat cctggcgaac 3361 aacctcagct tcttcttcta gggcgggtca tggcttggac gtacatcatg cggcagtccg 3421 acctgccgcc aggcgagatg cagcgccatg aaggcggccc cgagccggtg atggtctgca 3481 acgtcgacgg ggagttcttc gccgtgcagg acacgtgcac gcatggaaac tgggcgctat 3541 cggatggcta tctggacggt ggcgtcgtcg aatgcacgct gcatttcggc aagttctgcg 3601 ttcgaaccgg aaaggtgaag gcgctgccag cctgcaagcc gatcaaggtg tttcccatca 3661 aagtggaagg cggcgacgtg catgtcgatc ttgacgccgg cgaggtgaag tgatggcgac 3721 gcatgtggcg atcatcggca atggtgtggc gggctttacg accgcgcaag ccctgcgtgc 3781 tgaaggcttt gaggggcgga tctcgctgat cgggaacgaa ccgcatctgc cctatgaccg 3841 accgtctctg tccaaggccg tgctcggcgg cagtcttgag catccgcccg tattggcgga 3901 agccgactgg tacggcgagg cgcgcatcga catgctgtcc ggccgaagtg tcaccaacct 3961 gaacgtggac gctcgaacga tcagcctaga cgatggaagc acgttcgctg cagacgccat 4021 agtcatcgcc acgggcagcc gggcacgaac gcttgcgttg cctggcagcc aactgaccgg 4081 cgttgtcacg cttcgcacca acgacgacgt gcggccgctg tgccgtggct ggacgcctgc 4141 aacgcgactg gtgatcgcag gcggcggatt gatcggctgc gaagtcgcga cgacggcgcg 4201 caagctcggc ctggcggtga cgattctgga atccgccgac gaactgctgg tgcgtgtgct 4261 gggccggcgc atcggcgcct ggctgcgcgg cctgttgacg gaactcggcg tacgggtcga 4321 gctgggaaca ggcgtggccg gcttctctgg cgacgaccgg ctcgaagagg tactggccag 4381 cgatggacgc cgcttcgccg ccgacaacgc gcttgtctgc atcggcgccg agcccgagga 4441 tcaactcgcg cgtcaggcgg gcctgtcctg cgaccggggc gtcattgtcg atgaccacgg 4501 cgcgacgcat gccgaagggg tattcgcagt cggcgatgcg gccagttggc cgctgcgcga 4561 tggcggcagg cgttcgctcg aaacctacat gaacgcgcag cgccaggccg ccgcggtggc 4621 agcggccatt ctgggaaagc atggatcggc gccgcaggtg cccgtgtcct ggacggagat 4681 cgccgggcat cggatgcaga tggcgggcga tatcgaaggg ccgggcgaat tcgtcttgcg 4741 cggcacgctg ggggatggcg cggcgctgct gttccggctg cgggacggaa ggattcaggc 4801 cgtcgtcgcg gtcgacgcgc cgcgtgactt cgcgatggcg gcccgattgg tggaagcgcg 4861 tgcagccatc gagccggcgc ggctggcaga tttttcaaac agcatgcgcg atctcgtgcg 4921 tgcacaacaa ggagactcag catgaaactc aaaggtgaag tggcattggt gacgggcgga 4981 ggcgccggac tggggcgagc gatcgtcgat cgctatgtcg cagagggcgc ccgcgtcgcg 5041 gtgctggaca agtcggcagc aggcctggag gaaatcagga agcgccacgg cgatgccgtg 5101 gtcggcatcg agggcgatgt tcggtcgctc gacagccatc gtgaagccgt ggcgcgctgc 5161 gtcgagacct tcggaaagct cgattgcctg atcggcaacg ccggcgtttg ggactaccag 5221 acccaactgg cggacattcc cgacaacggc atctcggaag ccttcgacga aatgttcgcg 5281 atcatcgtca aaggctacat cctggccgcc aaggccgcgt tgcccgcgct gtacaagagc 5341 aaaggcagcg cgatcttcac cgtgtcgaac gctggcttct accctggtgg cggaggcgtt 5401 ctctacaccg cgggcaagca tgccgtgatc ggcttggtca agcagctcgc gcatgaatgg 5461 gggcctcgca tccgtgtcaa cggcatcgcc cctggaggga tcctcggcag cgacatacgc 5521 gggctcaaga cgctgggtct gcaagaccaa accattgcga cgatgccact ggccgacatg 5581 ctgggtcctg tccttccgac cgggcgggtg gccaccgccg aggaatacgc cggagcctac 5641 gtgttcttcg ccacgcgcgc ggataccgtc ccgctcaccg gcagcgtgct gaacatcgac 5701 ggcggcatgg gcgtgcgcgg cttgttcgaa gccagtctcg gtgcacagct cgacaagcat 5761 ttcgcttgaa ggagagacaa catgagcatt caaaggcttg gctatatggg cttcgaagtc 5821 gcggatgtgc cggcatggcg ctccttcgtt accagcaagc tgggggcgat ggaagcctcc 5881 gccaacgagg cggaggccac gttccggatc gactcgcgca cctagcggct atcggtcagc 5941 aagggcactg cggacgacta cctgtttgcc ggctacgagg tggacagcga gaaaggactg 6001 caagaagtca aggaaagcct ggaggcccac ggcgttgctg tcaaggtcga aggtcgggaa 6061 ctggccgcca agcgcggtgt gcttgggctt atttcctgta gcggacccgt tcggcaaccg 6121 cgtcgaggtc tactacggcg gcaccgagct gttcgagcgg ccgttcgctt cacccaccgg 6181 cgtcacggga ttccagaccg gcgaccaggg tatcggtcac tatgtactca gtgtcccgga 6241 cgtggacgca gcgctcgcct tctaaaccaa ggggctgggt ctccagcttg ccgacgtcat 6301 cgactggacg gtcggcgaag ggatgaccct gactgtctac ttcttctact gcaacggccg 6361 ccaccacact ttcgcatttg gcaagctgcc cgggccgaag aagctccatc acttcatgct 6421 gcaaaccaac agcatggacg acgttggctt ggcctacgac agattcgatg ctgaacgcgc 6481 gatcgtcatg acgctcggcc ggcatacgaa cgaccacatg gtttcgttct acggggccac 6541 gccttcgggc tttgcggtcg agtacggctg gggtgcccgt gagatgcccc cccgctggtc 6601 ggtcgtgcgc tacgaccgca tcagcatctg ggggcacaag ttccagccgc ccgcatgaac 6661 cgccagcctg gctggacg //