LOCUS       SRU60191                1294 bp    DNA     linear   BCT 08-JAN-1997
DEFINITION  Streptomyces roseosporus ribosomal protein S12 (rpsL) and ribosomal
            protein S7 (rpsG) genes, complete cds, and elongation factor G
            (fusA) gene, partial cds.
ACCESSION   U60191
VERSION     U60191.1
KEYWORDS    .
SOURCE      Streptomyces filamentosus (Streptomyces roseosporus)
  ORGANISM  Streptomyces filamentosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Hosted,T.J. and Baltz,R.H.
  TITLE     Use of rpsL for dominance selection and gene replacement in
            Streptomyces roseosporus
  JOURNAL   J. Bacteriol. 179 (1), 180-186 (1997)
   PUBMED   8981996
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Hosted,T.J. and Baltz,R.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-1996) Infectious Diseases, Eli Lilly and Company,
            Lilly Corporate Center, Indianapolis, IN 46285, USA
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Streptomyces filamentosus"
                     /mol_type="genomic DNA"
                     /strain="A21978.6"
                     /db_xref="taxon:67294"
     gene            96..689
                     /gene="rpsL"
     regulatory      96..125
                     /regulatory_class="promoter"
                     /gene="rpsL"
                     /note="possible promoter sequence"
     regulatory      310..313
                     /regulatory_class="ribosome_binding_site"
                     /gene="rpsL"
     CDS             318..689
                     /gene="rpsL"
                     /function="streptomycin target, confers streptomycin
                     sensitivity"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S12"
                     /protein_id="AAC44743.1"
                     /translation="MPTIQQLVRKGRQDKVEKNKTPALEGSPQRRGVCTRVFTTTPKK
                     PNSALRKVARVRLTSGIEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKNRKQARSRYGAKKEK"
     gene            681..1162
                     /gene="rpsG"
     regulatory      681..684
                     /regulatory_class="ribosome_binding_site"
                     /gene="rpsG"
     CDS             692..1162
                     /gene="rpsG"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S7"
                     /protein_id="AAC44744.1"
                     /translation="MPRKGPAPKRPVIIDPVYSSPLVTSLINKILLDGKRSTAERIVY
                     GAMEGLREKTGNDPVITLKRALENVKPSLEVKSRRVGGATYQVPIEVKPGRASTLALR
                     WLVGYSRARREKTMTERLMNELLDASNGLGASVKKREDTHKMAESNKAFAHYRW"
     gene            1201..1294
                     /gene="fusA"
     CDS             1201..>1294
                     /gene="fusA"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="AAC44745.1"
                     /translation="MATTSLDLAKVRNIGIMAHIDAGKTTTTERI"
BASE COUNT          246 a          443 c          399 g          206 t
ORIGIN      
        1 ggatccggca accgcgggga cggccggcgg ccaggcgttc cggcccgtcg ccggtggtcg
       61 gcggccctac cccgcgggcg tggacagcat ttgttttgac ccagctccgt gaggtaggta
      121 cgctcaagcc ttgtgcctgg ggtgtgcctg ggctcgtgtg cgtgtcctca accgcagcgc
      181 gagtccgtca gtggccaccg caatctgcgc cccttctgcc ccagggcggg agtccgcagt
      241 attcgacaca cccgaccgcg tgggtcggcg atgttccagg ttagtttcac gaacggcaca
      301 cagaaaccgg agaagtagtg cctacgatcc agcagctggt ccggaagggc cggcaggaca
      361 aggtcgagaa gaacaagacg cccgcgctcg agggttcgcc ccagcgtcgc ggtgtctgca
      421 cgcgtgtgtt cacgaccacc ccgaagaagc cgaactcggc gctccgtaag gtcgcgcgtg
      481 tgcgtctgac ctccggcatc gaggtcacgg cctacatccc gggtgaggga cacaacctgc
      541 aggagcactc catcgtgctc gtgcgtggtg gccgtgtgaa ggacctgccg ggtgttcgtt
      601 acaagatcat ccgcggttcg ctcgacaccc agggtgtcaa gaaccgcaag caggcccgca
      661 gccgctacgg cgccaagaag gagaagtaag aatgcctcgt aagggccccg ccccgaagcg
      721 cccggtcatc atcgacccgg tctacagctc tcctcttgtc acctcgctga tcaacaagat
      781 cctcctcgac ggcaagcgtt ccaccgccga gcggatcgtg tacggcgcca tggaaggcct
      841 ccgcgagaag accggcaacg acccggtcat cacgctgaag cgcgcgcttg agaacgtcaa
      901 gccctcgctc gaggtcaagt cccgccgtgt cggtggcgcc acctaccagg tgccgatcga
      961 ggtcaagccc ggtcgcgcct ccaccctcgc tctgcgctgg ctcgtcggtt actcccgcgc
     1021 ccgccgcgag aagaccatga ccgagcgcct catgaacgaa ctgctcgacg cctccaacgg
     1081 cctcggtgct tcggtcaaga agcgtgagga cacccacaag atggccgagt ccaacaaggc
     1141 cttcgcgcac taccgctggt agtcgctacc cccatcgaga ccgagagaag actgagcctt
     1201 atggccacca cttcgcttga cctggccaag gtccgcaaca ttgggatcat ggcccacatc
     1261 gacgcgggca agacgaccac cactgagcgg atcc
//