LOCUS       PPU31240                1831 bp    mRNA    linear   INV 11-JUN-1997
DEFINITION  Photuris pennsylvanica luciferase mRNA, complete cds.
ACCESSION   U31240
VERSION     U31240.1
KEYWORDS    Firefly luciferase.
SOURCE      Photuris pensylvanica (Pennsylania firefly)
  ORGANISM  Photuris pensylvanica
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga;
            Elateriformia; Elateroidea; Lampyridae; Photurinae; Photuris.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ye,L., Buck,L.M., Schaeffer,H.J. and Leach,F.R.
  TITLE     Cloning and sequencing of a cDNA for firefly luciferase from
            Photuris pennsylvanica
  JOURNAL   Biochim. Biophys. Acta 1339 (1), 39-52 (1997)
   PUBMED   9165098
  REMARK    Dr. Keith Wood of Promega Corporation has cloned two cDNAs for
            Photuris pennsylvanica from fireflies collected in Maryland.  One
            of the corresponding protein sequences differs by only two amino
            acids from that reported herein.
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Leach,F.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-1995) Biochemistry & Molecular Biology, Oklahoma
            State University, 246 B Noble Research Center, Stillwater, OK
            74078-3035, USA
COMMENT     On Jun 11, 1997 this sequence version replaced gi:927311.
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Photuris pensylvanica"
                     /mol_type="mRNA"
                     /db_xref="taxon:1710587"
     5'UTR           1..61
     CDS             62..1699
                     /EC_number="1.13.12.7"
                     /codon_start=1
                     /product="luciferase"
                     /protein_id="AAB60897.1"
                     /translation="MEDKNILYGPEPFHPLADGTAGEQMFYALSRYADISGCIALTNA
                     HTKENVLYEEFLKLSCRLAESFKKYGLKQNDTIAVCSENGLQFFLPLIASLYLGIIAA
                     PVSDKYIERELIHSLGIVKPRIIFCSKNTFQKVLNVKSKLKYVETIIILDLNEDLGGY
                     QCLNNFISQNSDINLDVKKFKPNSFNRDDQVALVMFSSGTTGVSKGVMLTHKNIVARF
                     SHCKDPTFGNAINPTTAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTFEEKLFLQSL
                     QDYKVESTLLVPTLMAFFPKSALVEKYDLSHLKEIASGGAPLSKEIGEMVKKRFKLNF
                     VRQGYGLTETTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGKILGPNETGELYFK
                     GDMIMKSYYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAP
                     AEIEGILLQHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTA
                     KWLRGGVKFLDEIPKGSTGKIDRKVLRQMFEKHKSKL"
     3'UTR           1700..>1831
     polyA_site      1831
BASE COUNT          615 a          281 c          353 g          582 t
ORIGIN      
        1 ggcacgagcc agacgggtct ggatagtttg atttaaatac ttaaagttac aagttgaaat
       61 aatggaagat aaaaatattt tatatggacc tgaaccattt catcccttgg ctgatgggac
      121 ggctggagaa cagatgtttt acgcattatc tcggtatgca gatatttcag gatgcattgc
      181 attgacaaat gctcatacaa aagaaaatgt tttatatgaa gaatttttaa aattgtcgtg
      241 tcgtttagcg gaaagtttta aaaagtatgg attaaaacaa aacgacacaa tagcggtgtg
      301 tagtgaaaat ggtttgcaat ttttccttcc tttaattgca tcattgtatc ttggaataat
      361 tgcagcacct gttagtgata aatacattga acgtgaatta atacacagtc ttggtattgt
      421 aaaaccacgc ataatttttt gttccaagaa tacttttcaa aaagtactga atgtaaaatc
      481 taaattaaaa tatgtagaaa ctattattat attagactta aatgaagact taggaggtta
      541 tcaatgcctc aacaacttta tttctcaaaa ttccgatatt aatcttgacg taaagaaatt
      601 taaaccaaat tcttttaatc gagacgatca ggttgcgttg gtaatgtttt cttctggtac
      661 aactggtgtt tctaagggag tcatgctaac tcacaagaat attgttgcac gattttctca
      721 ttgcaaagat cctacttttg gtaacgcaat taatccaacg acagcaattt taacagtaat
      781 accattccac catggttttg gtatgactac cacattagga tactttactt gtggattccg
      841 agttgctcta atgcacacgt ttgaagaaaa actattttta caatcattac aagattataa
      901 agtggaaagt actttacttg taccaacatt aatggcattt tttccaaaaa gtgcgttagt
      961 tgaaaagtac gatttatcgc acttaaaaga aattgcatct ggtggcgcac ctttatcaaa
     1021 agaaattggg gagatggtga aaaaacggtt taaattaaac tttgtcaggc aagggtatgg
     1081 attaacagaa accacttcgg ctgttttaat tacaccggac actgacgtca gaccgggatc
     1141 aactggtaaa atagtaccat ttcacgctgt taaagttgtg gatcctacaa caggaaaaat
     1201 tttggggcca aatgaaactg gagaattgta ttttaaaggc gacatgataa tgaaaagtta
     1261 ttataataat gaagaagcta ctaaagcaat tattaacaaa gacggatggt tgcgctctgg
     1321 tgatattgct tattatgaca atgatggcca tttttatatt gtggacaggc tgaagtcatt
     1381 aattaaatat aaaggttatc aggttgcacc tgctgaaatt gagggaatac tcttacaaca
     1441 cccgtatatt gttgatgccg gcgttactgg tataccggat gaagccgcgg gcgagcttcc
     1501 agctgcaggt gttgtagtac agactggaaa atatctaaac gaacaaatcg tacaaaattt
     1561 tgtttccagt caagtttcaa cagccaaatg gctacgtggt ggggtgaaat ttttggatga
     1621 aattcccaaa ggatcaactg gaaaaattga cagaaaagtg ttaagacaaa tgtttgaaaa
     1681 acacaaatct aagctgtaag gctacgtttc tacgttatct atttatctat ctacacgtta
     1741 tctaattatg tttgagttat aatttatttt atgtatatgt acaattattt atgcttctga
     1801 cttcatcgtc cattaaaagt ggggtgcaat a
//