LOCUS CLU24268 4229 bp DNA linear BCT 26-JUN-2002 DEFINITION Clostridium litorale glycine reductase complex selenoproteins A (grdA) and B (grdB), thioredoxin reductase (trxB), and thioredoxin (trxA) genes, complete cds. ACCESSION U24268 VERSION U24268.1 KEYWORDS . SOURCE Peptoclostridium litorale ORGANISM Peptoclostridium litorale Bacteria; Bacillota; Clostridia; Peptostreptococcales; Peptoclostridiaceae; Peptoclostridium. REFERENCE 1 (bases 1 to 4229) AUTHORS Kreimer,S., Soehling,B. and Andreesen,J.R. TITLE Site directed mutagenesis of thioredoxin from Clostridium litorale: the unusual Gly31 at the redox active site is essential for activity JOURNAL Unpublished REFERENCE 2 (bases 1 to 4229) AUTHORS Kreimer,S. and Andreesen,J.R. TITLE Glycine reductase of Clostridium litorale. Cloning, sequencing, and molecular analysis of the grdAB operon that contains two in-frame TGA codons for selenium incorporation JOURNAL Eur. J. Biochem. 234 (1), 192-199 (1995) PUBMED 8529640 REFERENCE 3 (bases 1 to 4229) AUTHORS Kreimer,S. and Andreesen,J.R. TITLE Direct Submission JOURNAL Submitted (10-APR-1995) Sylke Kreimer, Institut fuer Mikrobiologie, Georg-August-Universitaet, Grisebachstrasse 8, Goettingen, D-37077, Germany COMMENT On Jan 30, 1996 this sequence version replaced gi:924752. FEATURES Location/Qualifiers source 1..4229 /organism="Peptoclostridium litorale" /mol_type="genomic DNA" /db_xref="taxon:1557" regulatory 393..398 /regulatory_class="minus_35_signal" regulatory 424..429 /regulatory_class="minus_10_signal" regulatory 436..440 /regulatory_class="ribosome_binding_site" gene 450..926 /gene="grdA" CDS 450..926 /gene="grdA" /codon_start=1 /transl_except=(pos:579..581,aa:Sec) /transl_table=11 /product="glycine reductase complex selenoprotein A" /protein_id="AAC43573.2" /translation="MSLFDGKKVIIIGDRDGIPGPAMAECLKGINVEVVYSATECFVU TAAGAMDLENQNWVKNFTDQYGAENIIVLVGAAEAESAGLAAETVTAGDPTFAGPLAG VQLGLRVFHAVEPEFKGAVDSAIYDEQIGMMEMVLDVDSIIEEMKSIRADYCKFND" regulatory 932..936 /regulatory_class="ribosome_binding_site" gene 944..2254 /gene="grdB" CDS 944..2254 /gene="grdB" /codon_start=1 /transl_except=(pos:1991..1993,aa:Sec) /transl_table=11 /product="glycine reductase complex selenoprotein B" /protein_id="AAC43574.2" /translation="MGKLRVVHYINQFFAGIGGEEKADIAPFVAEELPPISQNLDKLL GEDAEVVATVVCGDSFFGENLETAQATVLEMVKGASPDLFIAGPAFNAGRYGVAAGAI TKAVQDTLGIPAVTGMYIENPGADMYKKSVYVMSTADSAAGMRKSLPALAKFALKYAK GEEIGSPAEEGYIERGIRVNGFKEDRGAKRAVAMLVKKLKGEEFVTEYPMPVFDNVVP GRAIVNMSKAKIAIVTSGGIVPKGNPDRIESSSASKYGKYDIDGVDDLTSEGWETAHG GHDPVYANEDADRVIPVDVLRDMEKEGVIGELHRYFYSTTGNGTAVLSSKQFAKEFTQ ELMAAGVDAVILTSTUGTCTRCGATMVKEIERSGIPVVHICTVTPIALTVGANRIVPA IAIPHPLGDPALSPAEEKALRRKIVEKSLKALETEIEEQTVFED" stem_loop 2298..2329 stem_loop 2326..2392 regulatory 2385..2390 /regulatory_class="minus_35_signal" regulatory 2405..2410 /regulatory_class="minus_10_signal" gene 2457..3417 /gene="trxB" regulatory 2457..2461 /regulatory_class="ribosome_binding_site" /gene="trxB" CDS 2470..3417 /gene="trxB" /codon_start=1 /transl_table=11 /product="thioredoxin reductase" /protein_id="AAC43575.1" /translation="MENVYDIAIIGSGPAGLAAALYGARAKMKTLLLEGMKVGGQIVI THEVANYPGSVPEATGPSLIGRMEEQVEEFGAERVMDNIVDVDFTDKIKVLKGAKGEY KAKAVIVATGASPKLAGCPGEKELTGKGVSYCATCDADFFEDMEVFVIGGGDTAVEEA MFLTKFARKVTIVHRRAELRAAKSIQEKAFKNEKLNFMWNTVIEEIKGDGIVESAVFK NRETGEVTEFVAPEEDGTFGIFVFIGYDPKSALVEGKLELDETGYIPTDDNMKTNVEG VFAAGDIRVKSLRQVVTATADGAIAAVQAEKYIEELFAE" gene 3519..3855 /gene="trxA" regulatory 3519..3523 /regulatory_class="ribosome_binding_site" /gene="trxA" CDS 3532..3855 /gene="trxA" /codon_start=1 /transl_table=11 /product="thioredoxin" /protein_id="AAC43576.1" /translation="MLMLDKDTFKTEVLEGTGYVLVDYFSDGCVPCKALMPAVEELSK KYEGRVVFAKLNTTGARRLAISQKILGLPTLSLYKDGVKVDEVTKDDATIENIEAMVE EHISK" stem_loop 3926..3958 BASE COUNT 1500 a 727 c 1014 g 988 t ORIGIN 1 gtacctagga cttgacggag ccatagtatc tcaggaaggt ttcggaaacc cagatacaga 61 ccttataatg aactgcaaga agatagaaat gcaagacgtt aagactgtaa tatctacaga 121 cgagtacgca ggaagagacg gagcttctca gtcgcttgct gatgctgacg ttagagctga 181 cgcagttgta tctaacggaa atgcaaacat ggtaataact cttcctaaga tggacaaggt 241 tataggacat atccagtaca tcgacactat agctggagga ttcgacggat cacttgcaga 301 agacggaagc atagtagtga gatccaggct atacagagct acaacgagct aggatttgga 361 tacctttcag ctaaaggata ctaatattag cctgtaaata gaaaaactca tgctttaata 421 aaatataaat cagaaggagc gactttggta tgtcattatt cgacggcaaa aaagtcatca 481 taatcggtga cagagacgga ataccaggtc ctgctatggc agaatgttta aagggaataa 541 atgtagaagt agtttattca gctactgagt gttttgtctg aacagctgct ggtgctatgg 601 acctagagaa ccaaaactgg gttaaaaact ttacagatca gtatggagca gagaacataa 661 tagttcttgt tggagcagcg gaagctgaat cagcaggact agctgcagag actgtaacag 721 caggagaccc tacattcgca ggtccattag caggagtcca gttgggacta agagtattcc 781 acgcagttga accagaattc aaaggagcag tagactcagc aatatacgat gagcaaattg 841 gaatgatgga aatggttctt gacgttgact ctataataga ggaaatgaag tcaataagag 901 ccgactactg caagttcaac gactaattaa gggaggtaaa atcatgggaa aattaagagt 961 agttcattat ataaaccagt tcttcgcagg aataggtgga gaagaaaaag ctgacatagc 1021 acctttcgta gcagaggagc ttccaccaat aagccaaaac cttgacaaac ttctaggaga 1081 agacgcagaa gtagtagcta cagtagtttg tggagactct ttcttcggag aaaaccttga 1141 aacagcacag gcaacagtac tagaaatggt aaagggtgca agccctgacc tattcatagc 1201 tggaccagca ttcaacgcag gaagatacgg agttgcagct ggagctataa ctaaggctgt 1261 tcaagacaca cttggaatac ctgctgttac aggaatgtac atcgagaacc ctggtgcaga 1321 catgtacaag aagagcgttt acgtaatgtc aacagctgac tcagctgcag gaatgagaaa 1381 gtctcttcca gcgctagcta agttcgctct taagtatgct aagggagagg aaataggatc 1441 tccagcagaa gaaggataca tcgaaagagg aataagagtc aacggattca aagaagacag 1501 aggagctaag agagctgtag ctatgcttgt taaaaagctt aaaggcgaag agttcgtaac 1561 tgagtaccca atgccagttt tcgacaacgt tgtgccagga agagctatag taaacatgtc 1621 aaaagctaaa atagcgatag taacttctgg tggtatagtt ccaaaaggaa acccagatag 1681 aatagagtca tcttcagcat ctaagtacgg aaagtatgac atagacggag tagacgatct 1741 tacttcagaa ggctgggaga cagctcacgg tggacacgac cctgtatacg ctaacgaaga 1801 tgcagacaga gttatacctg ttgacgttct tagagacatg gagaaagaag gagtaatagg 1861 agagcttcac agatacttct actctacaac aggaaacgga actgcagttc taagctctaa 1921 gcagtttgct aaagagttca ctcaagaact aatggcagcc ggagtagacg cagttatact 1981 tacttctacg tgaggcactt gtactcgttg cggcgcaacg atggttaaag aaatagaaag 2041 atcaggaata cctgtagttc acatatgtac agttactcca atagcactta cagttggagc 2101 taacagaata gttccagcaa tagctatacc tcacccacta ggagatccag ctctttctcc 2161 agcagaagag aaggctctta gaagaaagat agttgagaag tctcttaagg ctcttgaaac 2221 agaaatagaa gagcagacag tattcgaaga ctaataagta aacccggggg tagttcattc 2281 tatcctctcc ttaaaaaaca ggtgcgaaag ctttgccttt cacacctgta ataaaaagtt 2341 ttcaaggtat ctttaaaagc tgtaaagata ttttgaaaat gtctttgata agatcgagga 2401 cgcatataat tgtcagaggt tacataaaac aaaacataaa aagcaaacga ttttaaggag 2461 gaagctaaag tggaaaacgt atatgatata gccataatag gttcaggacc tgcaggactt 2521 gcagcagcac tttacggagc aagagcaaag atgaagactc ttcttcttga gggaatgaaa 2581 gttggaggac agatagtaat aactcacgag gttgcaaact atccaggatc agttccagaa 2641 gctacaggac cgtctctaat aggaagaatg gaagagcagg ttgaagaatt cggagcagaa 2701 agagtaatgg acaacatagt agacgttgat tttacagaca agataaaagt acttaaggga 2761 gcaaaaggcg agtacaaggc taaggctgta atagttgcta ctggagcatc tccaaagctt 2821 gcaggatgcc cgggagaaaa agagcttaca ggaaaaggcg tatcatactg cgcgacttgc 2881 gacgctgact tcttcgagga catggaagtg ttcgtaatag gcggaggaga cacagcagtt 2941 gaagaggcta tgttccttac aaaattcgct agaaaagtta caatagttca cagaagagcc 3001 gagcttagag ctgccaagtc tatacaagag aaggcattca aaaacgaaaa gcttaacttc 3061 atgtggaaca cagtaataga agaaataaag ggagacggaa tagtagaatc tgcagtattc 3121 aaaaatagag aaactggaga agttacagag ttcgttgcac ctgaagaaga cggcactttc 3181 ggaatattcg tattcatagg atacgatccc aaaagcgctc ttgtagaagg aaagctagag 3241 cttgacgaga caggctatat accaacagac gacaacatga agactaacgt tgaaggcgta 3301 tttgccgccg gagacataag agtcaagtca cttagacagg tagttacagc gacagctgac 3361 ggagctatag ctgcagttca agctgagaag tatatagaag agctcttcgc agagtaataa 3421 caataagtaa tgcaactaaa aaatgcagtt ataatatgat tgattcagca tggtccattt 3481 ggattgggct gataaattaa taaaaagcaa aaataaaagg agatgaatgc aatgttaatg 3541 ctagacaaag atactttcaa aacagaagtt cttgagggaa caggatatgt actagttgac 3601 tacttcagtg atggatgcgt accatgcaag gctcttatgc cagcggtaga agagctttcg 3661 aagaaatacg aaggaagagt tgtgtttgca aagctcaaca caacaggagc aagaagactt 3721 gctataagcc aaaagatact aggccttcct acactgtctc tatacaagga tggagtaaag 3781 gtggatgaag ttactaaaga cgatgcaaca atagaaaaca tagaggcaat ggtagaagaa 3841 catattagta aataaccagt attatttatg tgtgtaaaag gctgaatctc ataattttga 3901 aaattaaaaa aatacattga aaaacagggc aaagacatgt caaaacggtc tttgctctat 3961 tttgttttta gtggtatcat tgttatgtat ggggattgga aaatttaagg taaggggtgt 4021 ttgttttgaa aaatgaaaaa ttgaaaaagg ggctgatgac cactgtaggt gatatctggc 4081 gcgatttcat atctaattgg agcattggca cttaaagaag cttctcagac ctcaaaaatc 4141 attgtggcgg ctcttggaat ggctacaggt tttttaggat ggacattatc ggaaataaac 4201 acaaggtcct ccataaagca tatagctat //