LOCUS BJU12678 11715 bp DNA linear BCT 15-NOV-2023 DEFINITION Bradyrhizobium japonicum USDA 110 cytochrome P-450 BJ-2 (CYP115P) pseudogene, cytochrome P-450 BJ-1 (CYP112) gene, cytochrome P-450 BJ-3 (CYP114) gene, putative ferredoxin gene, putative sterol dehydrogenase-like enzyme gene, cytochrome P-450 BJ-4 (CYP117) gene, and putative dimethylallyltranstransferase gene, complete cds. ACCESSION U12678 L02323 L12971 VERSION U12678.1 KEYWORDS . SOURCE Bradyrhizobium diazoefficiens USDA 110 ORGANISM Bradyrhizobium diazoefficiens USDA 110 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Nitrobacteraceae; Bradyrhizobium. REFERENCE 1 (sites) AUTHORS Tully,R.E. and Keister,D.L. TITLE Cloning and Mutagenesis of a Cytochrome P-450 Locus from Bradyrhizobium japonicum That Is Expressed Anaerobically and Symbiotically JOURNAL Appl. Environ. Microbiol. 59 (12), 4136-4142 (1993) PUBMED 16349113 REFERENCE 2 (bases 1 to 11715) AUTHORS Tully,R.E., van Berkum,P., Lovins,K.W. and Keister,D.L. TITLE Identification and sequencing of a cytochrome P450 gene cluster from Bradyrhizobium japonicum JOURNAL Biochim. Biophys. Acta 1398 (3), 243-255 (1998) PUBMED 9655913 REFERENCE 3 (bases 1 to 11715) AUTHORS Tully,R.E. TITLE Direct Submission JOURNAL Submitted (26-JUL-1994) Raymond E. Tully, USDA/ARS, Soybean and Alfalfa Research Laboratory, Bldg. 011, HH-19, BARC-West, Beltsville, MD 20705, USA COMMENT On or before Aug 16, 1994 this sequence version replaced gi:294674, gi:294676. FEATURES Location/Qualifiers source 1..11715 /organism="Bradyrhizobium diazoefficiens USDA 110" /mol_type="genomic DNA" /strain="USDA 110" /type_material="type strain of Bradyrhizobium diazoefficiens" /db_xref="taxon:224911" regulatory 393..407 /regulatory_class="promoter" /citation=[2] /function="putative fnr-type promoter" gene 1276..1581 /gene="CYP115P" CDS 1276..1581 /gene="CYP115P" /note="cytochrome P-450 BJ-2; identified by a heme binding site and by sequence homology to the B. japonicum CYP112 and CYP114 genes" /pseudo /codon_start=1 /transl_table=11 misc_binding 1387..1416 /gene="CYP115P" /note="this heme binding site is in an apparently non-functional P-450 pseudogene" /bound_moiety="P-450-type heme" gene 1587..2803 /gene="CYP112" regulatory 1587..1590 /regulatory_class="ribosome_binding_site" /gene="CYP112" /note="putative ribosome binding site" CDS 1598..2803 /gene="CYP112" /experiment="experimental evidence, no additional details recorded" /note="start codon has been experimentally determined" /codon_start=1 /transl_table=11 /product="cytochrome P-450 BJ-1" /protein_id="AAC28889.1" /translation="MSEQQPLPTLPMWRVDHIEPSPEMLALRANGPIHRVRFPSGHEG WWVTGYDEAKAVLSDAAFRPAGMPPAAFTPDSVILGSPGWLVSHEGREHARLRAIVAP AFSDRRVKLLVQQVEAIAAHLFETLAAQPQPADLRRHLSFPLPAMVISALMGVLYEDH AFFAGLSDEVMTHQHESGPRSASRLAWEELRAYIRGKMRDKRQDPDDNLLTDLLAAVD QGKASEEEAVGLAAGMLVAGHESTVAQIEFGLHAMFRHPQQRERLVGDPSLVDKAVQE ILRMYPPGAGWDGIMRYPRTDVTIAGEHIPAESKVLVGLPATSFDPHHFDDPEIFDIE RQEKPHLAFSYGPHACIGVALARLELKVVFGSIFQRLPALRLAVAPEQLKLRKEIITG GFEQFPVLW" misc_binding 2624..2653 /gene="CYP112" /note="putative heme binding site for P-450 CYP112" /bound_moiety="P-450-type heme" gene 3039..4187 /gene="CYP114" CDS 3039..4187 /gene="CYP114" /standard_name="cytochrome P-450" /note="start codon has not been determined experimentally" /codon_start=1 /transl_table=11 /product="cytochrome P-450 BJ-3" /protein_id="AAC28890.1" /translation="MLSRHADIFWAFKATGDAFRGPAPGELARYFSRAATSPSLNLLA STLAMKDPPTHTRLRRLISRDFTMGQIDNLRPSIARIVAARLDGITPALERGEAVDLH REFALALPMLVFAELFGMPQDDMFELAAGIGTILEGLGPHASDPQLAAADAASARVQA YFGDLIQRKRTDPRRDIVSMLVGAHDDDADTLSDAELISMLWGMLLGGFVTTAASIDH AVLAMLAYPEQRHWLQADAARVRAFVEEVLRCDAPAMFSSIPRIAQRDIELGGVVIPK NADVRVLIASGNRDPDAFADPDRFDPARFYGTSPGMSTDGKIMLSFGHGIHFCLGAQL ARVQLAESLPRIQARFPTLAFAGQPTREPSAFLRTFRTLPVRLHAQGS" misc_binding 4002..4031 /gene="CYP114" /note="putative heme binding site for P-450 CYP114" /bound_moiety="P-450-type heme" regulatory 4180..4183 /regulatory_class="ribosome_binding_site" /gene="CYP114" /note="putative" CDS 4189..4473 /note="ORF3; similar to the Streptomyces griseolus 3Fe-4S ferredoxins Fd-1 and Fd-2, Swiss-Prot Accession Numbers P18324 and P18325, which also occur downstream of P-450 genes (O'Keefe et al., Biochemistry 30:447-455(1991)" /codon_start=1 /transl_table=11 /product="putative ferredoxin" /protein_id="AAC28891.1" /translation="MRVMVDQDLCGTSGQCVLTLPGTFRQREPDGVAEVCVATVPHAL HAAVRLAASQCPVAHSGHRKRRWRWRARQAPTLRLLQRRPCGMPRKTSTI" regulatory 4472..4475 /regulatory_class="ribosome_binding_site" /note="putative" CDS 4483..5310 /note="ORF4; similar to several bacterial sterol dehydrogenases: GenBank Accession Numbers D23722 and D10497, and Swiss-Prot Accession" /codon_start=1 /transl_table=11 /product="putative sterol dehydrogenase-like enzyme" /protein_id="AAC28892.1" /translation="MGRFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADIDGSAAI ACTAQIAAEAGHALALAIDIADAQAVAALFETAERHFGGVDLLVNNASAMHLTPRDRA ILELELAVWDQTMARNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTALTSYA ASKAAMNMLSSSLATQYGHAQIRCNAVAPGLIMTERLRMQTHLRRHQLLPRVGRPRTW PRWWRSCSPTMLRSSTGQVVCIDGGMLAHVPTYADGGNSRAARPAGETAEADAAPRC" gene 5564..6652 /gene="CYP117" regulatory 5564..5568 /regulatory_class="ribosome_binding_site" /gene="CYP117" /note="putative RBS" CDS 5582..6652 /gene="CYP117" /codon_start=1 /transl_table=11 /product="cytochrome P-450 BJ-4" /protein_id="AAC28893.1" /translation="MFGGTLVAQDGIAHRQARDAIQAALLPKGLTLAGIGELFAPVIR ARVQRWRERGDVTILRETGDLMLKLIFSLMGIPAQDLPGWHRKYRQLLQLIVAPPVDL PGLPLRRGRAARDWIDARLREFVRAAREHASRTGLINDMVSAFDRSDDALSDDVLVAN IRLLLLGGHDTTASTMAWMVIELARQPGLWDALVEEAQRVGAVPTRHADLAQCPVAEA LFRETLRVHPATPLLVRRALRELRIGQQRIPTGTDLCIPLLHFSTSALLHEAPDQFRL ARWLQRTEPIRPVDMLQFGTGPHFCMGYHLVWLELVQFCIALALTMHEAGVRPRLLSG VEKGRRYYPTAHPSMTIRIGFS" misc_binding 6461..6490 /gene="CYP117" /note="putative heme binding site for P-450 CYP117" /bound_moiety="P-450-type heme" regulatory 6792..6796 /regulatory_class="ribosome_binding_site" /note="putative RBS for ORF6" CDS 6807..7805 /note="putative dimethylallyltranstransferase; ORF6; similar to E. coli dimethylallyltranstransferase, Swiss-Prot Accession Number P22939 (Fujisaki et al., J. Biochem. 108:995-1000(1990)" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AAC28894.1" /translation="MQTGSTPHDDRAGVSANGILGAQARGASGRLLPEIWMQDGAKRV EEALARLLCAEDDGETELMAAMRYATLHGGKRTRALLCLAAGALADTPAHMLDDVGAA IEMMHACTLVHDDLPAMDDDVLRRGLPTVHVKFGEATAILVGDALQAHAFLTLASLNA PGDSPIALVRELAQAVSAEGAAGGQAIDLSLVGKHVELDRIVAMHRMKSGALVRASVR MGALCAVGVNAAHAALYCALDHYSACFGLALQVIDDILDVTADTAALGKTPGKDAAAQ KPTCASIMGLQEARQFALDLLRDAGEAIAPLGPRAERLAQLIQRANAYLFKHAPRA" regulatory 7936..7939 /regulatory_class="ribosome_binding_site" /note="putative RBS for ORF7" CDS 7948..9498 /note="ORF7; contains a motif similar to the 'QW' motif that occurs in squalene and oxidosqualene cyclases (Poralla et al., Trends in Biochem. Sci. 19:157-158(1994))" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AAC28895.1" /translation="MNALSEHILSELRRLLSEMSDGGSVGPSVYDTAQALRFHGNVTG RQDAYAWLIAQQQADGGWGSADFPLFRHAPTWAALLALQRADPLPGAADAVQTATRFL QRQPDPYAHAVPEDAPIGAELILPQFCGEAAWLLGGVAFPRHPALLPLRQACLVKLGA VAMLPSGHPLLHSWEAWGTSPTTACPDDDGSIGISPAATAAWRAQAVTRGSTPQVGRA DAYLQMASRATRSGIEGVFPNVWPINVFEPCWSLYTLHLAGLFAHPALAEAVRVIVAQ LEARLGVHGLGPALHFAADADDTAVALCVLHLAGRDPAVDALRHFEIGELFVTFPGER NASVSTNIHALHALRLLGKPAAGASAYVEANRNPHGLWDNEKWHVSWLYPTAHAVAAL AQGKPQWRDERALAALLQAQRDDGGWGAGRGSTFEETAYALFALHVMDGSEEATGRRR IAQVVARALEWMLARHAAHGLPQTPLWIGKELYCPTRVVRVAELAGLWLALRWGRRVL AEGAGAAP" regulatory 9483..9487 /regulatory_class="ribosome_binding_site" /note="putative RBS for ORF8" CDS 9495..10397 /note="ORF8" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AAC28896.1" /translation="MIQTERAVQQVLEWGRSLTGFADEHAVEAVRGGQYILQRIHPSL RGTSARTGRDPQDETLIVTFYRELALLFWLDDCNDLGLISPEQLAAVEQALGQGVPCA LPGFEGCAVLRASLATLAYDRRDYAQLLDDTRCYSAALRAGHAQAVAAERWSYAEYLH NGIDSIAYANVFCCLSLLWGLDMATLRARPAFRQVLRLISAIGRLQNDLHGCDKDRSA GEADNAVILLLQRYPAMPVVEFLNDELAGHTRMLHRVMAEERFPAPWGPLIEAMAAIR VQYYRTSTSRYRSDAVRGGQRAPA" BASE COUNT 1935 a 3831 c 3882 g 2067 t ORIGIN 1 gtcgacaatc acgacgcaaa ggatgcagtg agttgggcct tcaggccgcg gtgattgacg 61 ccttctgtga aggcgcggat gcgtcggccg gcgcttgcgt tcggtcgcgg cgggggcaaa 121 tctgcagcgg atagccggct gcgtgccggc ttgcgagtgt gccgacatcg aaagcggggg 181 caccccgcta tccatgacgg ccggcacaaa ttgcaattct gtttcacaat ggccgcctgc 241 ggtttgctca actgacgtcg ccaagaaaca attgctgcgg cgacaatcca tgccgtccgc 301 tatcgaatca cgcgcttgat ccagaccgac tgtgcgcaga atatgaattt ccacgacatc 361 agctttcgtt tgaagcacgc taagccgacg cattgatatt ggtccagctg tgactcttac 421 aagcagcttc atcctgaaaa gccaaagtct tgattgacaa agcaccttat cttcgaacgg 481 tgaaagcgtc ggagttttcc acattgccag ggtgcccctg caggacctga ggggcgcgac 541 tccagcgatt gcgccgctgc gggcggcgcc acagaatgca ccccggcgct ggtcgctaat 601 ctaatgcgcc ctacgctaaa ggccagaacc gagaggcatc tccgtagcta cttttggaag 661 cgtttgttca ggaagtgcgt cgctcccatg cgagccttca cgtgccgaac ggatgctcga 721 ctgtctcgcg agctggcgca tcgcctctgg gtttgcatca aggcgctgct gcacggcctc 781 gagcagatgc tcatgcttgc gtgcttccag agtgcctatc ttgtccttca acttcgctgc 841 cggcaccccc tcaagcatag cttgaatcgc cttatctagc tcgcgctcag tccactcccg 901 gcatcgtgac gctgcgaccg cgccccgtcc ccgagcttcc gcatcttcgg tagccagagc 961 cacgaaggcg gcgaggtctt gccctccgga caggctgacg cgatcgcgtt cgatgacgcc 1021 ggcgtcatcg gagtggtttc cggcgaacca cacctgctgc agccaatccg cttgaaattt 1081 cgcgcaaggg ctgccccgca cattcagcgc gagagcaggc ctttgtcggg cacttactgc 1141 gcccgttgtc ggcattgaga ctgaatgcca gaaacctgac actacgcgca gctatgtagt 1201 gcctgctgag cctgcgaatt cacttcggca cgcgatttgc tctgttcacg gtgtgggacc 1261 ggtccggagg ctataatggc ggacacatcg ggggcacggt cgaacctcct tcaccaatcc 1321 gtacggtcga tctatggcga tgccgacagg ttcgatgtca cccggcggca caacccgcac 1381 ctgtcgttcg gccaggggcc gcatttctgc ctgggcgcag cgctggcgcg tctggaattg 1441 ggctgcgcct tccccgcgct gttcgtgcgg ctggagcatc tggcgctgac catcgccgcg 1501 gaggacgtcg tctacatgcc gtcttacgtc attcgctgcc cgcagcgcct gccggtcacc 1561 ttccgccctt ccatcgctta gagagtgaag tgccaccatg tccgaacaac aacccttgcc 1621 gacgctgccg atgtggcgcg tcgatcacat cgagccctcg cccgagatgt tggcgctacg 1681 cgccaacggt ccgatccacc gcgtgcgctt cccctccggg cacgaaggct ggtgggtgac 1741 aggctatgac gaggccaagg cggtgctgtc cgacgcggcg ttccggcccg cgggaatgcc 1801 gccggcggca ttcaccccgg attcggtgat tctcggttcg ccggggtggc tggtctcgca 1861 cgaggggcgc gagcatgccc ggctacgcgc gatcgtggcg ccggccttca gtgaccgcag 1921 ggtgaagctg ctcgtgcagc aggtcgaagc gatcgccgcg cacttgttcg agacgctggc 1981 ggcccagccc cagcccgccg acctgcggcg ccacctctcc tttccgcttc cggccatggt 2041 catcagcgcg ctgatgggcg tgctctacga ggatcacgcc tttttcgccg ggctgtccga 2101 tgaggtgatg acgcaccagc atgaaagcgg cccgcgcagc gcgtcccgcc tggcctggga 2161 agaactgcgc gcctacattc gcggcaagat gcgcgacaag cgccaggatc cggacgacaa 2221 cctgctgacg gatctgctcg ctgcggtcga ccagggcaag gcgagcgagg aagaggcggt 2281 tggcctggcg gcgggcatgc tggtggcggg gcacgagagc accgtcgcgc agatcgaatt 2341 cggcctgcat gccatgttcc gccatccgca acagcgcgaa cgcctggtcg gcgatccgtc 2401 cctggtcgac aaggcggtac aggaaatcct gcgcatgtac ccgccgggcg cgggctggga 2461 tggcatcatg cgctatccga ggaccgacgt gaccatcgcg ggcgagcata ttcccgcgga 2521 gagcaaggtg ctggtcggcc tcccggcaac gtcgttcgat ccgcaccatt tcgacgatcc 2581 ggaaatcttc gatatcgaac gccaggaaaa gccgcacctg gctttttcct acgggccgca 2641 cgcgtgcatc ggcgtggcgc tggccaggct ggagctcaag gtggtgttcg gttcgatctt 2701 ccagcgcctt cccgcgctgc gcctggccgt ggcgccggaa caactgaagt tgcgcaagga 2761 gatcatcacg ggcgggttcg agcagttccc ggtgctctgg tgatgtcgcg gacgccacgg 2821 gcgatctcga tcttctccgg aatctgccgc gcgcctgtcg cgcgccggtc agatcagccc 2881 gccaacaggt aaccaggatg gacgtgcaag aaaccacggc agcatgccgg gacgccttcg 2941 ccgaactggc gtcgccagcg tgcatccacg acccgtatcc gttcatgcga tggttgcgcg 3001 agcacgatcc ggtgcatcgc gcggcgtcgg gcctcttttt gttgagccgc cacgccgaca 3061 tcttctgggc gttcaaggcc acgggcgatg cgtttcgggg tccggcgccc ggcgaactgg 3121 cgcgctattt ctcgcgtgcg gcgaccagcc cgtcgctcaa tctgttggcg tccacgctag 3181 cgatgaagga cccaccgacg catacgcgtc tgcgccggct gatctcgcgc gatttcacca 3241 tgggccagat cgacaacctg cggccgagca tcgcgcgcat cgtcgcagcg cgcctggacg 3301 gcataacgcc cgcgctggag cgcggggagg cggtggacct gcatcgggaa ttcgcgctgg 3361 ccttgcccat gctggttttc gccgaactgt tcggcatgcc ccaagacgac atgttcgagc 3421 tcgctgccgg catcggcacc attctggaag gcctgggccc gcacgccagc gatccccagc 3481 tcgccgcggc ggacgcggcc agcgccaggg tgcaggccta cttcggcgac ctcatacagc 3541 gcaagcgcac cgatccccgc cgcgacatcg tgtcgatgct ggtcggcgca catgacgacg 3601 atgccgacac actgtcggat gcggagttga tcagcatgct gtggggcatg ctgctgggcg 3661 gcttcgtcac cactgctgcg agcatcgacc atgccgtcct ggcgatgctg gcgtatcccg 3721 aacagcggca ctggctgcag gcagacgccg cgcgggtgag ggcattcgtc gaagaagtcc 3781 tgcgctgcga cgcgcccgcc atgttcagct ccatcccgcg tatcgcccag cgcgacatcg 3841 aactgggcgg cgtggtgatt ccgaagaacg cggacgtgcg cgtgctgatc gcgtccggca 3901 atcgcgaccc ggacgccttc gccgatcccg atcgcttcga tcccgcgcgg ttctacggca 3961 ccagtccagg catgtcgacc gacgggaaga tcatgctgag cttcggccac ggcattcact 4021 tctgcctcgg tgcgcaactg gcccgggtgc agttggccga gagcctgccg cggatccagg 4081 cccgcttccc cacgctggca tttgccgggc agccgacccg ggagccctcg gcattccttc 4141 ggacgttccg cacgctgccg gtgcggctgc atgcgcaggg gagctgagat gcgcgtcatg 4201 gtcgaccagg atctgtgcgg aaccagcggg cagtgcgtgc tgacgctgcc gggcaccttt 4261 cgccagcgcg aaccggacgg cgtggccgaa gtgtgcgtgg cgacggtccc gcatgcgctg 4321 cacgccgccg tgcggctcgc ggccagccag tgcccggtcg cgcattcggg tcatcgaaag 4381 cgacgctggc gatggcgagc gcgccaggcg ccgaccctgc gccttctcca gcggaggccg 4441 tgcggcatgc cgcgaaagac cagcacaatc taggatgaca cgatgggacg gtttgaaggc 4501 aaagtggccg tggtgaccgg cgccggcgcc ggcatcggca aggcatgcgc cctcgccatc 4561 gcgcgcgagg gcggcagagt ggtggtggcc gacattgatg gctcggcggc tatcgcctgc 4621 accgcgcaga tcgcggccga agcgggccac gcgctcgccc tggcgatcga catcgccgat 4681 gcgcaggcgg tggcagcgct gttcgagacg gcggagcggc acttcggcgg ggtcgacctg 4741 ctggtgaaca acgcgagcgc gatgcatctg accccgcgcg accgcgcgat cctcgagctg 4801 gagctagcgg tctgggatca gaccatggcg aggaatctgc gtggcacgct gctctgctgc 4861 cggcaggcca tcccccggat gatcgcccgc ggcggtggcg cgatcgtcaa catgtcgtcg 4921 tgccaggggc tcagtggtga caccgcactg acatcctacg ccgcgtcgaa ggcggcgatg 4981 aacatgctgt cgtcctcact tgccacccag tacggtcatg cgcagatccg ctgcaatgcg 5041 gttgcgccgg gtctcatcat gaccgagcga ctccgcatgc aaacccatct gcgccgtcac 5101 cagctcctgc cgcgcgtcgg ccgcccgagg acgtggccgc gctggtggcg ttcctgctct 5161 ccgacgatgc tgcgttcatc aactggccag gtcgtgtgca tcgacggcgg catgctggcg 5221 catgtgccga catacgccga cggtggcaac agccgcgccg cgcggcctgc cggcgagacc 5281 gcagaagcgg acgcggcgcc gcgctgctga tggacatgct gctcaacccg ctgaaccgtc 5341 ggcatcggct gcggcacgac atcccggtcg tgcccggcgc ttttcccttg gtcgggcatc 5401 ttcccgccgt cgtctgcgac ctgccgcgcc tgctgcggcg tgcggaacgg acgctgggca 5461 gccacttctg gctagatttc ggccctgccg gacacctgat gaccagtctg gatccggatg 5521 ctctcgcact gctccggcac aaggacgtgt cctcgggctg atcgaagaca tcgcgcccga 5581 attgtttggc ggaacgttgg tcgcccagga cggcatcgcg caccggcagg cgcgcgatgc 5641 gatccaggcg gcgcttctgc ccaaggggct gaccctggcc ggcatcggcg agctgttcgc 5701 gcccgtcatc cgggcgcgcg tgcagaggtg gcgggaacgg ggcgacgtca ccatcctgcg 5761 cgaaaccggc gatctaatgc tcaagctcat cttcagtctc atgggaatcc ccgcgcagga 5821 cctgccggga tggcatcgca agtaccggca actgctgcag ttgatcgtcg cgcccccggt 5881 cgacctgcct ggactgccct tgcggcgggg ccgcgcggcc cgcgactgga tcgatgcgcg 5941 cttgcgcgag ttcgtccgcg ccgcgcgcga acatgcctcg cgcaccgggt tgatcaacga 6001 catggtgagc gccttcgatc gcagcgacga tgcgctttcc gatgacgtcc tggtcgccaa 6061 tattcgcttg ctgctgcttg gtggtcacga caccaccgcc tcgacgatgg cctggatggt 6121 gatcgagcta gcgcggcagc ctgggctgtg ggacgccctg gtcgaggagg cgcaacgcgt 6181 aggcgcggtg ccgactcggc acgcggacct ggcgcagtgt ccggtcgctg aggcgctgtt 6241 ccgcgagacg ctgcgcgtgc atccggcgac gccgctccta gtgcgtcgcg cactgcgtga 6301 attgcgaatc ggccaacagc gcattcctac gggcaccgat ctgtgcatcc cgctgctgca 6361 tttctcgacc tcggcgctgc tgcacgaggc gcctgatcag tttcggctgg cgcggtggct 6421 tcaacgcacc gagccgatcc ggccggtgga catgctgcag ttcggtaccg gcccacactt 6481 ctgcatgggc taccacctgg tatggctgga actggtgcag ttctgtatcg ccttggcgct 6541 gaccatgcac gaggccgggg tgcggccgcg gttgctgagc ggcgtcgaaa aaggccggcg 6601 ctattacccg accgcacatc cgtccatgac tatccgcatc ggattctcat gagctggcat 6661 cgcatgcccc gtgtggtgag gcagcggcgc cggcgacgcg cggcattgct gcactcggcg 6721 cgcgcgctcg gtgcgaggcc ggcaacaccg cggcggctcg ccgctcgccg tggccgtctc 6781 ctggcaggta caaggacatg aacaacatgc agactggttc cacgccacac gacgaccgag 6841 ctggcgtttc cgcgaacggc atattgggcg cgcaggcgcg cggcgcatcc ggcaggctgc 6901 tgcccgagat ctggatgcag gacggcgcaa agcgggtcga agaggcgctg gcgcgtcttc 6961 tctgcgccga agacgacggt gagaccgagc tgatggcggc gatgcgctac gccaccttgc 7021 atggcggcaa gcgcacccgc gccttgctct gtttggctgc cggcgcactg gccgacacgc 7081 cggcgcacat gctcgatgac gtcggtgccg ccatcgagat gatgcacgcc tgtacgttgg 7141 tccacgacga tctgcccgcg atggacgacg acgtgcttcg ccgcggcctt ccgaccgtgc 7201 acgtcaagtt cggcgaagcc accgcgatcc tggtcggcga tgcgctgcag gcgcacgcct 7261 tcctgaccct ggcgagcctg aatgcgccgg gcgacagccc tatcgcgctc gtgcgcgaac 7321 tggcgcaggc ggtgtccgcc gagggggccg caggcgggca ggccatagat ctgtcgctgg 7381 ttggaaagca cgtcgagctg gacaggatcg tggcgatgca ccggatgaag agcggagcgc 7441 tagtgcgcgc gtccgttcgc atgggcgcgc tatgcgctgt cggcgtgaat gccgcgcacg 7501 ctgcacttta ctgtgcgctg gatcactaca gcgcctgttt cggcctggcg ttacaggtga 7561 tcgacgacat tctcgacgtg acagcggata ccgcggcgct gggcaagacc cccggcaagg 7621 acgcggcggc gcagaagccg acctgcgcgt cgatcatggg actgcaggaa gcacgccagt 7681 tcgcgctgga tctgttgcgc gatgccggcg aggccatcgc gccgctgggg ccgcgtgcgg 7741 aacggttggc gcaactcata cagcgggcca acgcgtatct gttcaaacac gcgccacgcg 7801 catgagcgcg cccgtccgca tcgagtcgcc cctgtgccgc tgcgatcggc cggcggcaga 7861 gtgcatcctg cactgtgtcc gagtccgcgg cgccgatcgc agcggttgcc cgccgcggcg 7921 cacgtttctg caaacggaac atcccgcgtg aacgcgctgt ccgaacatat cctttccgaa 7981 ttgcgccgcc tgctgagcga aatgagcgat ggcggcagcg tcggtccgtc cgtctacgac 8041 acggcgcagg cgctgcgctt ccacggcaac gtcaccggtc ggcaggacgc atacgcgtgg 8101 ctcatcgcgc agcaacaggc cgacggcgga tggggaagcg cggacttccc gctgttccgc 8161 catgcgccca cgtgggcggc gttactggca ttgcagcgtg ccgatcctct tcccggagct 8221 gcggacgcag tccagactgc aacgaggttc ctccagcgcc agcccgatcc ctacgcacat 8281 gcggtgccag aagacgcgcc gatcggcgcg gagctgatcc tgccgcagtt ttgcggtgag 8341 gccgcatggt tgctgggtgg cgtagcgttt ccgcgccatc ctgcgctgtt gccattgcgg 8401 caagcgtgcc tggtcaagct gggggcggtg gcgatgttgc cgagcggcca tccgttgcta 8461 cactcctggg aagcctgggg gacgtcgccg accaccgcat gcccggatga cgacggcagc 8521 atcggcatca gtccggcggc caccgccgcg tggcgtgccc aggccgtgac acgggggagc 8581 acgccgcagg tcgggcgcgc cgatgcgtat ctgcagatgg catcgcgggc gacgcgcagc 8641 ggcatcgaag gtgtctttcc caacgtctgg ccgatcaatg tgttcgagcc atgctggtcg 8701 ctgtacaccc tgcatctggc cgggcttttc gcgcatcccg cgctcgcgga ggcggttcgc 8761 gtgatcgtcg cgcagctcga ggcccgtctg ggcgtgcacg gtctgggccc ggccttgcac 8821 ttcgcggctg atgcggacga caccgccgtt gcgttgtgcg tcctgcacct tgcaggccgt 8881 gacccggcgg tcgatgcgtt gcgccatttc gaaatcggcg agctgttcgt caccttcccc 8941 ggcgaacgca atgcctcggt gtcgaccaac attcatgccc tgcatgcgtt gcgactgttg 9001 ggaaagcccg ccgcgggcgc cagcgcgtac gtcgaggcca atcgcaaccc gcacggtcta 9061 tgggacaacg aaaaatggca cgtttcgtgg ctgtatccca ccgcgcatgc ggtcgctgcg 9121 ctggcgcaag gcaagcccca gtggcgagat gagcgcgcgc tggcggcgct gctgcaggcg 9181 cagcgcgacg acggtggctg gggcgcgggt cgcgggtcca cgttcgagga aaccgcctat 9241 gcgctgtttg cgttgcacgt gatggatggg agcgaagagg cgacagggcg ccggcgcatc 9301 gcgcaggtgg tggcgcgtgc gctggagtgg atgctcgccc gccatgcggc gcatggattg 9361 ccgcagacgc cgctgtggat cggcaaggaa ctgtattgcc ccactcgggt cgtgcgcgtg 9421 gccgaactcg ccgggttgtg gctggcgctt cgttgggggc ggcgcgtcct ggccgagggg 9481 gcaggagcgg cgccatgatc cagactgaac gcgcggtgca gcaggtgctg gagtggggac 9541 gttccctgac cggattcgcc gacgagcatg ccgtggaagc ggtcaggggc ggccagtaca 9601 tcctgcagcg catccacccg agcctgcgcg gcaccagcgc ccgcaccggt cgcgatccgc 9661 aggacgaaac gctgatagtg acgttctatc gcgaactggc gctgctgttc tggctcgacg 9721 attgcaacga ccttggcctg atctcgccgg aacagctcgc cgcggtggag caagcgctgg 9781 ggcagggcgt gccgtgcgcg ctccccggat tcgagggttg cgctgtgctg cgcgcttcgc 9841 tggccacgct cgcctacgat cgtcgcgatt atgctcagct tctcgacgat acccggtgct 9901 actccgcggc gctgcgcgcc ggacacgcgc aggcggtagc ggcggaacgc tggtcctacg 9961 ctgagtacct gcacaacggc atcgattcga tcgcctacgc gaacgtgttc tgttgcctgt 10021 ccttgctgtg ggggctggat atggcgacct tgcgcgcgcg tccggcgttt cgccaggtcc 10081 tgcggctcat ctccgcgata gggcgtctgc agaacgatct gcatggatgc gacaaggaca 10141 ggtcggccgg cgaggccgac aacgcggtga tcttgctgct gcagcgctat ccagctatgc 10201 ctgtggtgga gttcctcaac gacgagctgg ccggccatac gcgcatgctg caccgggtga 10261 tggcggaaga acgctttccc gcgccgtggg gaccattgat cgaggccatg gcggccatcc 10321 gcgtgcagta ctaccggacc tcgaccagcc gctaccgcag cgacgctgtg aggggaggcc 10381 agcgcgcgcc ggcctgaacg cgcaggaggc ggcggcgtgc gcgcgctgcc gtcggcccga 10441 tccgacgcaa cgccgccgcc cgatggagcg agcatgagcg acaccgccct gagactgcac 10501 gtccacgacc agttcgccaa cggccggcgc ttccgcattc tcaacatcgt cgacgacgtc 10561 actaaggaat acctgggcgc cattccggag acgtcaatcc ggggcggcgg gtggcccgcg 10621 aactgaagga aatcgttgag cgacgcggca agccaggaat gatcgcgtcc gaccatggca 10681 ccgagttcac ctgcaacgcc atgctggcct ggagcaagga cacggtcatc gactggcact 10741 tcatcgcgcc gggaaagccg atgcagaacg gcttcatcga gagattcatg tatagacggc 10801 cgcgcggatg caagggattt cagcagcaat ttggatatcg gtcgggtgcg ttcgtgtata 10861 cggcctatca ttgcgaccga tagcatggcc gctcgccctg atggagatcg cggatcgagg 10921 cctcatcaac gggtcgcgct tgttgcgctt aaaactcttc tgtcaatcgg ccctttaatt 10981 gcggattatt gtcaactctc tttttttgaa gccattcggc aagacggtag cgctcgacct 11041 ccccgtcggc ccgattcggc gcgcgccaat tgtgcacaca atcggcgaga aatgggggag 11101 cgtgctacga cctgttcaca gccctttctg tcgcgggcca tgtcgcgccg agcagccggg 11161 agttgttggc aatcgctgga attatcgggt aattttgcgc aacaggcgca acacgatgtc 11221 gacgaacaaa tgcggactaa gttattgaaa taattggtga tcccagcaga acaagcacat 11281 cagccggtat caatggctta gattggcaaa ccgcccgttc gggcaagatt gatccgcaag 11341 cgctttttcg cgcactggca atcaaaagcc gccggatggt gggccaaaaa tgcgaagggc 11401 cgcctcggct aaggcagccc cagatcgcaa tcaaaagtcc agtagcctgg aatttagcac 11461 ccagtctccc gagccgcaat ccggaaccgc catgcaagct gccttcgccg ctgccctggc 11521 gcggaagatg gtgcggccgt gagtgcaatt ctcgacaagg accggacccg gcgaaagctc 11581 catctgttaa cgcgcgcaaa tgcgatctac gccttaccga tcaagacttc cgggtgcttt 11641 ggtatatcgc cgatgcgatc gatcacgaga gaccgatcta gcccgccgca agcaggccac 11701 catcgctaaa gatct //