LOCUS       PFU04048                1795 bp    DNA     linear   BCT 08-OCT-1994
DEFINITION  Pseudomonas fluorescens biovar I benzaldehyde lyase (bznB) gene,
            complete cds.
ACCESSION   U04048
VERSION     U04048.1
KEYWORDS    .
SOURCE      Pseudomonas fluorescens
  ORGANISM  Pseudomonas fluorescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Hinrichsen,P., Gomez,I. and Vicuna,R.
  TITLE     Cloning and sequencing of the gene encoding benzaldehyde lyase from
            Pseudomonas fluorescens biovar I
  JOURNAL   Gene 144 (1), 137-138 (1994)
   PUBMED   8026749
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Hinrichsen,P.
  TITLE     Degradacion bacteriana de compuestos tipo 1-2,diariletanos
  JOURNAL   Thesis (1993) Lab. Plant Biotechnology, INIA - La Platina
REFERENCE   3  (bases 1 to 1795)
  AUTHORS   Hinrichsen,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-1993) Patricio Hinrichsen, INIA - La Platina,
            Lab. Plant Biotechnology, Santa Rosa 11610, Santiago, Chile
COMMENT     On Feb 3, 1994 this sequence version replaced gi:436828.
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Pseudomonas fluorescens"
                     /mol_type="genomic DNA"
                     /strain="biovar I"
                     /isolate="A 1"
                     /db_xref="taxon:294"
                     /clone="pUC19F5H"
                     /clone_lib="pLAFRI-1"
     5'UTR           1..103
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[1]
     gene            104..1795
                     /gene="bznB"
     CDS             104..1795
                     /gene="bznB"
                     /function="cleavage of benzoin-anisoin acyloin linkage"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[1]
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="benzaldehyde lyase"
                     /protein_id="AAA50176.1"
                     /translation="MAMITGGELVVRTLIKAGVEHLFGLHGAHIDTIFQACLDHDVPI
                     IDTRHEAAAGHAAEGYARAGAKLGVAGHGGRGIYQCGHAHCQRLAGSQGRCIPHPGSG
                     ALRDDETNTLQAGIDQVAMAAPITKWAHRVMATEHIPRLVMQAIRAALSAPRGPVLLD
                     LPWDILMNQIDEDSVIIPDLVLSAHGARPDPADLDQALALLRKAERPVIVLGSEASRT
                     ARKTALSAFVAATGVPVFADYEGLSMLSGLPDAMRGGLVQNLYSFAKADAAPDLVLML
                     GARFGLNTGHGSGQLIPHSAQVIQVDPDACELGRLQGIALGIVADVGGTIEALAQATA
                     QDAAWPDRGDWCAKVTDLAQERYASIAAKSSSEHALHPFHASQVIAKHVDAGVTVVAD
                     GALTYLWLSEVMSRVKPGGFLCHGYLGSMGVGFGTALGAQVADLEAGRRTILVTGDGS
                     VGYSIGEFDTLVRKQLPLIVIIMNNQSWGATLHFQQLAVGPNRVTGTRLENGSYHGVA
                     AAFGADGYHVDSVESFSAALAQALAHNRPACINVAVALDPIPPEELILIGMDPFA"
BASE COUNT          319 a          525 c          589 g          362 t
ORIGIN      
        1 cgccaagcaa tcgggctttg gggcagaatt gggtcgcgaa gggcttgagg agtttgccca
       61 gtccaagatc atcaacgccg cgctataaat taaaggatcc cccatggcga tgattacagg
      121 cggcgaactg gttgttcgca ccctaataaa ggctggggtc gaacatctgt tcggcctgca
      181 cggcgcgcat atcgatacga tttttcaagc ctgtctcgat catgatgtgc cgatcatcga
      241 cacccgccat gaggccgccg cagggcatgc ggccgagggc tatgcccgcg ctggcgccaa
      301 gctgggcgtg gctggtcacg gcgggcgggg gatttaccaa tgcggtcacg cccattgcca
      361 acgcttggct ggatcgcaag gccggtgtat tcctcacccg ggatcgggcg cgctgcgtga
      421 tgatgaaacc aacacgttgc aggcggggat tgatcaggtc gccatggcgg cgcccattac
      481 caaatgggcg catcgggtga tggcaaccga gcatatccca cggctggtga tgcaggcgat
      541 ccgcgccgcg ttgagcgcgc cacgcgggcc ggtgttgctg gatctgccgt gggatattct
      601 gatgaaccag attgatgagg atagcgtcat tatccccgat ctggtcttgt ccgcgcatgg
      661 ggccagaccc gaccctgccg atctggatca ggctctcgcg cttttgcgca aggcggagcg
      721 gccggtcatc gtgctcggct cagaagcctc gcggacagcg cgcaagacgg cgcttagcgc
      781 cttcgtggcg gcgactggcg tgccggtgtt tgccgattat gaagggctaa gcatgctctc
      841 ggggctgccc gatgctatgc ggggcgggct ggtgcaaaac ctctattctt ttgccaaagc
      901 cgatgccgcg ccagatctcg tgctgatgct gggggcgcgc tttggcctta acaccgggca
      961 tggatctggg cagttgatcc cccatagcgc gcaggtcatt caggtcgacc ctgatgcctg
     1021 cgagctggga cgcctgcagg gcatcgctct gggcattgtg gccgatgtgg gtgggaccat
     1081 cgaggctttg gcgcaggcca ccgcgcaaga tgcggcttgg ccggatcgcg gcgactggtg
     1141 cgccaaagtg acggatctgg cgcaagagcg ctatgccagc atcgctgcga aatcgagcag
     1201 cgagcatgcg ctccacccct ttcacgcctc gcaggtcatt gccaaacacg tcgatgcagg
     1261 ggtgacggtg gtagcggatg gtgcgctgac ctatctctgg ctgtccgaag tgatgagccg
     1321 cgtgaaaccc ggcggttttc tctgccacgg ctatctaggc tcgatgggcg tgggcttcgg
     1381 cacggcgctg ggcgcgcaag tggccgatct tgaagcaggc cgccgcacga tccttgtgac
     1441 cggcgatggc tcggtgggct atagcatcgg tgaatttgat acgctggtgc gcaaacaatt
     1501 gccgctgatc gtcatcatca tgaacaacca aagctggggg gcgacattgc atttccagca
     1561 attggccgtc ggccccaatc gcgtgacggg cacccgtttg gaaaatggct cctatcacgg
     1621 ggtggccgcc gcctttggcg cggatggcta tcatgtcgac agtgtggaga gcttttctgc
     1681 ggctctggcc caagcgctcg cccataatcg ccccgcctgc atcaatgtcg cggtcgcgct
     1741 cgatccgatc ccgcccgaag aactcattct gatcggcatg gaccccttcg catga
//