LOCUS ECU00734 6039 bp DNA linear BCT 24-MAY-1995 DEFINITION Escherichia coli acrA, acrB, and acrR genes, complete cds. ACCESSION U00734 VERSION U00734.1 KEYWORDS lipoprotein; integral membrane polypeptide; drug susceptibility. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 6039) AUTHORS Ma,D., Cook,D.N., Alberti,M., Pon,N.G., Nikaido,H. and Hearst,J.E. TITLE Molecular cloning and characterization of acrA and acrE genes of Escherichia coli JOURNAL J. Bacteriol. 175 (19), 6299-6313 (1993) PUBMED 8407802 REFERENCE 2 (bases 1 to 6039) AUTHORS Alberti,M. TITLE Direct Submission JOURNAL Submitted (13-AUG-1993) Marie Alberti, Structural Biology, U.C. Lawrence Berkeley Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA FEATURES Location/Qualifiers source 1..6039 /organism="Escherichia coli" /mol_type="genomic DNA" /db_xref="taxon:562" /clone="[150]19B7, [151]8C4" /clone_lib="Kohara lambda library of E. coli genome" gene complement(307..954) /gene="acrR" CDS complement(307..954) /gene="acrR" /function="potential regulator protein for acrAE genes" /codon_start=1 /transl_table=11 /product="25 kDa protein" /protein_id="AAA67133.1" /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE" regulatory 1085..1089 /regulatory_class="ribosome_binding_site" gene 1096..2289 /gene="acrA" CDS 1096..2289 /gene="acrA" /function="an IS2 insertional mutation in E. coli strain N43 causes hypersusceptibility to hydrophobic drugs" /codon_start=1 /transl_table=11 /product="42 kDa protein" /protein_id="AAA67134.1" /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP EQSKS" sig_peptide 1096..1170 /gene="acrA" /note="The first 25 amino acids show features typical of a prokaryotic lipoprotein signal peptide including the L-T-G-C- consensus processing site for prelipoprotein precursors." mat_peptide 1171..2286 /gene="acrA" /product="unnamed" regulatory 2297..2301 /regulatory_class="ribosome_binding_site" gene 2312..5461 /gene="acrB" CDS 2312..5461 /gene="acrB" /function="putative transporter protein" /note="formally called acrE; hydropathy plot suggests that acrB encodes an integral membrane protein with characteristics of transporter proteins.; No rho-independent termination signal separates this gene from acrA preceding it; therefore it is likely acrA and acrB are on the same operon." /codon_start=1 /transl_table=11 /product="114 kDa protein" /protein_id="AAA67135.1" /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR RRFSRKNEDIEHSHTVDHH" regulatory 5479..5502 /regulatory_class="terminator" BASE COUNT 1503 a 1458 c 1589 g 1489 t ORIGIN 1 agatcttttt gtttatttag tgagtcaagt tgcgcctgca ggtccgcttt tgtcggcaga 61 tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc ataacaagac aaaaacaaaa 121 gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt aatactggaa catagtcatg 181 atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa gtcaaaccgc aagaatatca 241 cgacgcagtg aaccagaata gcaacgacga aaatgtccag gaaaaatcct ggagtcagat 301 tcagggttat tcgttagtgg caggattacg aagcgtgggg cacaggagat acatctccag 361 taagatggca acgtaatcgc gggcttcttt tttaagatca aaagattgcg gggcaaagag 421 ccagttttcc atcaggccgg aaatatagcc gcgcataata attgctgcgc gacgcgtcat 481 taaatccgca ggcaacattt tcgcttcaat acaatgtttt aacgtttgtt ctatacggtc 541 ataactttcc agacagagat tacgttgtgc ctgttgcaca acagccattt ctccgacaaa 601 ttcgcatttg tggaatataa tctccatcaa taatcgacgc cgttcttctg tcaccgtgga 661 ttcaagaaca tgaattaata tctctcttaa tactgagagt ggatcgccag ggaattttgc 721 ctgatactca agctctagtt caccaatatt ggattctgac agttcccaga tctcactgaa 781 caaatccgac ttgtctttaa aatgccagta gattgcaccg cgcgtaacgc cagctgcttt 841 tgcaatctcg cccagcgagg tggatgatac cccctgctgt gagaaaagac gtagagccac 901 atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg gtttttcgtg ccatatgttc 961 gtgaatttac aggcgttaga tttacataca tttgtgaatg tatgtaccat agcacgacga 1021 taatataaac gcagcaatgg gtttattaac ttttgaccat tgaccaattt gaaatcggac 1081 actcgaggtt tacatatgaa caaaaacaga gggtttacgc ctctggcggt cgttctgatg 1141 ctctcaggca gcttagccct aacaggatgt gacgacaaac aggcccaaca aggtggccag 1201 cagatgcccg ccgttggcgt agtaacagtc aaaactgaac ctctgcagat cacaaccgag 1261 cttccgggtc gcaccagtgc ctaccggatc gcagaagttc gtcctcaagt tagcgggatt 1321 atcctgaagc gtaatttcaa agaaggtagc gacatcgaag caggtgtctc tctctatcag 1381 attgatcctg cgacctatca ggcgacatac gacagtgcga aaggtgatct ggcgaaagcc 1441 caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt atcagaaact gctcggtact 1501 cagtacatca gtaagcaaga gtacgatcag gctctggctg atgcgcaaca ggcgaatgct 1561 gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga tcaatctggc ttacaccaaa 1621 gtcacctctc cgattagcgg tcgcattggt aagtcgaacg tgacggaagg cgcattggta 1681 cagaacggtc aggcgactgc gctggcaacc gtgcagcaac ttgatccgat ctacgttgat 1741 gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg aactggcgaa tggcacgctg 1801 aaacaagaga acggcaaagc caaagtgtca ctgatcacca gtgacggcat taagttcccg 1861 caggacggta cgctggaatt ctctgacgtt accgttgatc agaccactgg gtctatcacc 1921 ctacgcgcta tcttcccgaa cccggatcac actctgctgc cgggtatgtt cgtgcgcgca 1981 cgtctggaag aagggcttaa tccaaacgct attttagtcc cgcaacaggg cgtaacccgt 2041 acgccgcgtg gcgatgccac cgtactggta gttggcgcgg atgacaaagt ggaaacccgt 2101 ccgatcgttg caagccaggc tattggcgat aagtggctgg tgacagaagg tctgaaagca 2161 ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc ctggtgtcca ggtaaaagca 2221 caagaagtta ccgctgataa taaccagcaa gccgcaagcg gtgctcagcc tgaacagtcc 2281 aagtcttaac ttaaacagga gccgttaaga catgcctaat ttctttatcg atcgcccgat 2341 ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg gggctggcga tcctcaaact 2401 gccggtggcg caatatccta cgattgcacc gccggcagta acgatctccg cctcctaccc 2461 cggcgctgat gcgaaaacag tgcaggacac ggtgacacag gttatcgaac agaatatgaa 2521 cggtatcgat aacctgatgt acatgtcctc taacagtgac tccacgggta ccgtgcagat 2581 caccctgacc tttgagtctg gtactgatgc ggatatcgcg caggttcagg tgcagaacaa 2641 actgcagctg gcgatgccgt tgctgccgca agaagttcag cagcaagggg tgagcgttga 2701 gaaatcatcc agcagcttcc tgatggttgt cggcgttatc aacaccgatg gcaccatgac 2761 gcaggaggat atctccgact acgtggcggc gaatatgaaa gatgccatca gccgtacgtc 2821 gggcgtgggt gatgttcagt tgttcggttc acagtacgcg atgcgtatct ggatgaaccc 2881 gaatgagctg aacaaattcc agctaacgcc ggttgatgtc attaccgcca tcaaagcgca 2941 gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg ccggtgaaag gccaacagct 3001 taacgcctct attattgctc agacgcgtct gacctctact gaagagttcg gcaaaatcct 3061 gctgaaagtg aatcaggatg gttcccgcgt gctgctgcgt gacgtcgcga agattgagct 3121 gggtggtgag aactacgaca tcatcgcaga gtttaacggc caaccggctt ccggtctggg 3181 gatcaagctg gcgaccggtg caaacgcgct ggataccgct gcggcaatcc gtgctgaact 3241 ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt gtttacccat acgacaccac 3301 gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg ctggtcgaag cgatcatcct 3361 cgtgttcctg gttatgtatc tgttcctgca gaacttccgc gcgacgttga ttccgaccat 3421 tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt gccgcctttg gcttctcgat 3481 aaacacgcta acaatgttcg ggatggtgct cgccatcggc ctgttggtgg atgacgccat 3541 cgttgtggta gaaaacgttg agcgtgttat ggcggaagaa ggtttgccgc caaaagaagc 3601 tacccgtaag tcgatggggc agattcaggg cgctctggtc ggtatcgcga tggtactgtc 3661 ggcggtattc gtaccgatgg ccttctttgg cggttctact ggtgctatct atcgtcagtt 3721 ctctattacc attgtttcag caatggcgct gtcggtactg gtggcgttga tcctgactcc 3781 agctctttgt gccaccatgc tgaaaccgat tgccaaaggc gatcacgggg aaggtaaaaa 3841 aggcttcttc ggctggttta accgcatgtt cgagaagagc acgcaccact acaccgacag 3901 cgtaggcggt attctgcgca gtacggggcg ttacctggtg ctgtatctga tcatcgtggt 3961 cggcatggcc tatctgttcg tgcgtctgcc aagctccttc ttgccagatg aggaccaggg 4021 cgtgtttatg accatggttc agctgccagc aggtgcaacg caggaacgta cacagaaagt 4081 gctcaatgag gtaacgcatt actatctgac caaagaaaag aacaacgttg agtcggtgtt 4141 cgccgttaac ggcttcggct ttgcgggacg tggtcagaat accggtattg cgttcgtttc 4201 cttgaaggac tgggccgatc gtccgggcga agaaaacaaa gttgaagcga ttaccatgcg 4261 tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt ttcgccttta acctgcccgc 4321 aatcgtggaa ctgggtactg caaccggctt tgactttgag ctgattgacc aggctggcct 4381 tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt gcagaagcag cgaagcaccc 4441 tgatatgttg accagcgtac gtccaaacgg tctggaagat accccgcagt ttaagattga 4501 tatcgaccag gaaaaagcgc aggcgctggg tgtttctatc aacgacatta acaccactct 4561 gggcgctgca tggggcggca gctatgtgaa cgactttatc gaccgcggtc gtgtgaagaa 4621 agtttatgtc atgtcagaag cgaaataccg tatgctgccg gatgatatcg gcgactggta 4681 tgttcgtgct gctgatggtc agatggtgcc attctcggcg ttctcctctt ctcgttggga 4741 gtacggttcg ccgcgtctgg aacgttacaa cggcctgcca tccatggaaa tcttaggcca 4801 ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg atggaacaac tggcgagcaa 4861 actgcctacc ggtgttggct atgactggac ggggatgtcc tatcaggaac gtctctccgg 4921 caaccaggca ccttcactgt acgcgatttc gttgattgtc gtgttcctgt gtctggcggc 4981 gctgtacgag agctggtcga ttccgttctc cgttatgctg gtcgttccgc tgggggttat 5041 cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat gacgtttact tccaggtagg 5101 cctgctcaca accattgggt tgtcggcgaa gaacgcgatc cttatcgtcg aattcgccaa 5161 agacttgatg gataaagaag gtaaaggtct gattgaagcg acgcttgatg cggtgcggat 5221 gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc ctcggcgtta tgccgctggt 5281 tatcagtact ggtgctggtt ccggcgcgca gaacgcagta ggtaccggtg taatgggcgg 5341 gatggtgacc gcaacggtac tggcaatctt cttcgttccg gtattctttg tggtggttcg 5401 ccgccgcttt agccgcaaga atgaagatat cgagcacagc catactgtcg atcatcattg 5461 atacaacgtg taatcactaa ggccgcgtaa gcggcctttt ttatgcataa cctacgaaca 5521 ttaaggagta attgaaccac caactcagga tctcatacga aaaccagtat taaccacgga 5581 taaaattcat aaaaaatact gattgttagt taatttatat taagtagcgc taatagattt 5641 aataatccat aatcatttag aggctattct taattatttg cggtaattct ttattcattc 5701 ctcggttatt acgtcatatt cagagcaatc ctggtattag tgtcaccaat ttcatctggc 5761 gataatcctg aaatgttatg aatagttcga gcaaactgct tttacctgct gcgggttagt 5821 gctagtatga aaaagtgagt cctgtcccgc ttccttccta attgtaattt ttcgtaataa 5881 tgcgatgaaa acctgcaaag agtggcttat agttaagcta acaaacgaga gggcaagtcc 5941 aggtcagtaa gttttttcca tcccgaaagg tgtccgttag ttcaaccgct aagaagggga 6001 cgcgttatgg atgaatactc acccaaaaga catgatatc //