LOCUS RFKB01000647 1261 bp DNA linear ENV 29-OCT-2018 DEFINITION Caldilineae bacterium isolate J117 k99_257391, whole genome shotgun sequence. ACCESSION RFKB01000647 RFKB01000000 VERSION RFKB01000647.1 DBLINK BioProject: PRJNA392119 BioSample: SAMN10120017 KEYWORDS WGS. SOURCE Caldilineae bacterium (hot springs metagenome) ORGANISM Caldilineae bacterium Bacteria; Chloroflexi; Caldilineae. REFERENCE 1 (bases 1 to 1261) AUTHORS Ward,L.M., Idei,A., Nakagawa,M., Ueno,Y., Fischer,W. and Mcglynn,S.E. TITLE Thermophilic Lithotrophy and Phototrophy in an Intertidal, Iron-rich, Geothermal Spring JOURNAL Unpublished REFERENCE 2 (bases 1 to 1261) AUTHORS Ward,L.M., Mcgglynn,S.E. and Fischer,W. TITLE Direct Submission JOURNAL Submitted (02-OCT-2018) Earth and Planetary Sciences, Harvard University, 24 Oxford Street, Cambridge, MA 02138, USA COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: MegaHit v. 1.1.2 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 28.1205241x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 10/24/2018 20:47:31 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 4,414 CDS (total) :: 4,371 Genes (coding) :: 4,305 CDS (coding) :: 4,305 Genes (RNA) :: 43 rRNAs :: 2, 2 (16S, 23S) partial rRNAs :: 2, 2 (16S, 23S) tRNAs :: 38 ncRNAs :: 1 Pseudo Genes (total) :: 66 Pseudo Genes (ambiguous residues) :: 0 of 66 Pseudo Genes (frameshifted) :: 2 of 66 Pseudo Genes (incomplete) :: 61 of 66 Pseudo Genes (internal stop) :: 5 of 66 Pseudo Genes (multiple problems) :: 2 of 66 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..1261 /organism="Caldilineae bacterium" /mol_type="genomic DNA" /isolate="J117" /isolation_source="iron-rich hot spring" /db_xref="taxon:2420332" /environmental_sample /geo_loc_name="Japan: Tokyo Prefecture, Shikinejima Island, Jinata Onsen" /lat_lon="34.318 N 139.216 E" /collection_date="2016" /note="metagenomic; derived from metagenome: hot springs metagenome" gene complement(1..66) /locus_tag="D6790_07975" /pseudo CDS complement(1..66) /locus_tag="D6790_07975" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_009783904.1" /note="incomplete; partial in the middle of a contig; missing start and stop; Derived by automated computational analysis using gene prediction method: Protein Homology." /pseudo /codon_start=1 /transl_table=11 /product="isopentenyl pyrophosphate isomerase" gene complement(62..709) /locus_tag="D6790_07980" CDS complement(62..709) /locus_tag="D6790_07980" /inference="COORDINATES: ab initio prediction:GeneMarkS+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="RME61329.1" /translation="MFQHSAASAIPVDLPIPRERTSLLQGLGRLERRLPLPRTWREYL AWLMVMGLLTALAGLQVSTALRVQKTRIEIQALRMEYESIQRQNAQLLWEISQHTNLE QVAQRAEALGFRPTLQRKFLGAPGAPIQAQAPDPAPLPTTTDASPALVEPFVPPNSEP QGLIPPAVLQGAAEQARLVQGWATRQLSGLATHFGPLSAPVRELWQAWFGDKVTG" gene complement(815..>1261) /gene="rsmH" /locus_tag="D6790_07985" CDS complement(815..>1261) /gene="rsmH" /locus_tag="D6790_07985" /EC_number="2.1.1.199" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_010936121.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase" /protein_id="RME61330.1" /translation="RSRRIARAIVAHRPLHTTQELARVIEQAVGGRRGERIHPATRTF QALRIAVNDELGQLERVLPQILDLLKSGGRVAVISFHSLEDRIIKQWMRQEAQAYTPD PTHPTGGRSRTPRLRLLTRRPIVAGDDEIARNPRSRSAKLRLAEKV" BASE COUNT 194 a 402 c 424 g 241 t ORIGIN 1 cccaatctcc caatctcctt gtctccctgt ctccttgtct ccttgtctcc ctgtctcccc 61 atcaccccgt caccttgtca ccaaaccacg cctgccaaag ctcccgcacc ggggcggaaa 121 gtgggccgaa gtgggttgcc aagccgctca gctggcgcgt cgcccacccc tgcacaagcc 181 tggcctgctc ggcagcgccc tggaggacag ccggcgggat gagtccttgg ggttcgctgt 241 tcggcgggac gaaaggttcg accagcgctg gggacgcgtc ggttgttgtg gggaggggcg 301 cagggtctgg cgcttgggcc tggattgggg cgccgggtgc gccgagaaat ttgcgctgca 361 aggtgggccg gaaacccagg gcctccgccc gttgcgccac ctgttccagg ttcgtgtgtt 421 ggctgatctc ccacaggagc tgggcgtttt gccgctggat gctctcgtac tccatacgca 481 gagcctggat ctcgatccgg gttttctgga cgcgcagagc cgtggagacc tggagaccgg 541 ccagagccgt cagcaggccc atcaccatga gccacgccag gtactcgcgc caggtgcggg 601 gcaggggcag ccttcgttcc aggcgcccaa ggccctggag caggcttgtg cgttcgcgtg 661 gaatcggcag atcaacagga atggctgagg cggcgctgtg ttggaacatg ggcttcactc 721 tttcagttca atgacagttc aagaccacag acggacgcag atgaacgctg gctccgcacg 781 cagacttctt tgcgctcttt gcggtttcaa aaaattacac cttttcggcc aggcgcagct 841 tggcggagcg gctgcgtggg ttgcgtgcga tctcgtcgtc tccggccaca atggggcgcc 901 gggtgagcag acgcaggcga ggcgtccggc tgcggcctcc ggtgggatgg gtcgggtcag 961 gggtgtaggc ctgggcctcc tgacgcatcc actgtttgat gatccggtcc tccaggctgt 1021 gaaagctgat caccgccacc cgcccgcccg acttcaacag gtccagaatc tggggcaaca 1081 cccgctccag ttggccgagt tcatcgttga cggcgatgcg cagggcctga aaggtgcggg 1141 tggccggatg gatgcgctct ccccggcggc cccccacggc ctgctcgatc accctggcca 1201 gctcctgggt ggtgtggaga ggccggtgcg ccacaatggc ccgagcgatg cgccggctgc 1261 g //