LOCUS       MZ318361               36942 bp    DNA     linear   PHG 25-JUN-2021
DEFINITION  Escherichia phage T7 isolate T7_del_holin_F1, complete genome.
ACCESSION   MZ318361
VERSION     MZ318361.1
KEYWORDS    .
SOURCE      Escherichia phage T7 (T7)
  ORGANISM  Escherichia phage T7
            Viruses; Duplodnaviria; Heunggongvirae; Uroviricota;
            Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus;
            Teseptimavirus T7.
REFERENCE   1  (bases 1 to 36942)
  AUTHORS   Xu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2021) National Research Center of Veterinary
            Biologicals Engineering and Technology, Jiangsu Academy of
            Agricultural Sciences, Zhongling Street, Nanjing, Jiangsu 210014,
            China
COMMENT     This T7 phage was purchased from Merck.  The holin gene (gene 17.5)
            was deleted from the genome and the phage was then serial passaged
            to the 20th generation.  Samples were take after the initial
            deltion (T7_del_holin_F1) the 10th generation (T7_del_holin_F10)
            and the 20th generation (T7_del_holin_F20).  These sequence are
            available in accessions MZ318361-MZ318363.
            
            ##Assembly-Data-START##
            Assembly Method       :: abyss v. 1.3.9
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36942
                     /organism="Escherichia phage T7"
                     /mol_type="genomic DNA"
                     /isolate="T7_del_holin_F1"
                     /host="Escherichia coli BL21"
                     /db_xref="taxon:10760"
                     /note="holin gene deltion mutant"
     regulatory      224
                     /regulatory_class="promoter"
                     /note="E. coli promoter A0 (leftward)"
     regulatory      405
                     /regulatory_class="promoter"
                     /note="T7 promoter phiOL"
     regulatory      498
                     /regulatory_class="promoter"
                     /note="E. coli promoter A1"
     regulatory      945
                     /regulatory_class="promoter"
                     /note="E. coli C promoter"
     misc_structure  970
                     /note="RNase III site R1"
     gene            971..3719
                     /locus_tag="T7delholinF1_07"
                     /note="gene 1"
                     /db_xref="GeneID:1261050"
     CDS             1003..3654
                     /locus_tag="T7delholinF1_07"
                     /note="A family of single subunit RNA polymerases."
                     /codon_start=1
                     /transl_table=11
                     /product="T3/T7-like RNA polymerase"
                     /protein_id="QWY13882.1"
                     /db_xref="GOA:P00573"
                     /db_xref="UniProtKB/Swiss-Prot:P00573"
                     /db_xref="GeneID:1261050"
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESY
                     EMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTA
                     FQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAK
                     HFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIE
                     MLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPK
                     PWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKV
                     LAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARK
                     SRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGK
                     PIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSP
                     FCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSET
                     VQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYG
                     VTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIW
                     ESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYK
                     KPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWA
                     HEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHES
                     QLDKMPALPAKGNLNFRDILESDFAFA"
     regulatory      3680
                     /regulatory_class="promoter"
                     /locus_tag="T7delholinF1_07"
                     /note="T7 promoter phi1.1A"
     misc_structure  3719
                     /locus_tag="T7delholinF1_07"
                     /note="RNase III site R1.1"
     gene            3720..4280
                     /locus_tag="T7delholinF1_08"
                     /note="gene 1.2"
                     /db_xref="GeneID:1261049"
     regulatory      3755
                     /regulatory_class="promoter"
                     /locus_tag="T7delholinF1_08"
                     /note="T7 promoter phi1.1B"
     gene            3839..3967
                     /locus_tag="T7delholinF1_09"
                     /note="gene 1.1"
                     /db_xref="GeneID:1261072"
     CDS             3839..3967
                     /locus_tag="T7delholinF1_09"
                     /note="other names: gp1.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13883.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03779"
                     /db_xref="GeneID:1261072"
                     /translation="MRNFEKMTKRSNRNARDFEATKGRKLNKTKRDRSHKRSWEGQ"
     CDS             3969..4226
                     /locus_tag="T7delholinF1_08"
                     /note="inhibits activity of the host dGTPase [dgt].
                     Essential only in strains that overexpress dGTPase [optA1
                     mutation]. In T7, gp1.2 also causes F plasmid exclusion.
                     In T3, however, gp1.2 overcomes the exclusion system.
                     Other names: dGTP triphosphohydrolase inhibitor; gp1.2."
                     /codon_start=1
                     /transl_table=11
                     /product="host dGTPase inhibitor"
                     /protein_id="QWY13884.1"
                     /db_xref="GOA:P03780"
                     /db_xref="UniProtKB/Swiss-Prot:P03780"
                     /db_xref="GeneID:1261049"
                     /translation="MGRLYSGNLAAFKAATNKLFQLDLAVIYDDWYDAYTRKDCIRLR
                     IEDRSGNLIDTSTFYHHDEDVLFNMCTDWLNHMYDQLKDWK"
     regulatory      4241
                     /regulatory_class="promoter"
                     /locus_tag="T7delholinF1_08"
                     /note="T7 promoter phi1.3"
     misc_structure  4280
                     /locus_tag="T7delholinF1_08"
                     /note="RNase III site R1.3"
     CDS             4307..5386
                     /note="Catalyzes the ATP-dependent formation of a
                     phosphodiester bond at the site of single-stranded breaks
                     in double-stranded DNA. T7 ligase is essential in
                     ligase-deficient hosts only."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="QWY13885.1"
                     /db_xref="GOA:P00969"
                     /db_xref="UniProtKB/Swiss-Prot:P00969"
                     /translation="MMNIKTNPFKAVSFVESAIKKALDNAGYLIAEIKYDGVRGNICV
                     DNTANSYWLSRVSKTIPALEHLNGFDVRWKRLLNDDRCFYKDGFMLDGELMVKGVDFN
                     TGSGLLRTKWTDTKNQEFHEELFVEPIRKKDKVPFKLHTGHLHIKLYAILPLHIVESG
                     EDCDVMTLLMQEHVKNMLPLLQEYFPEIEWQAAESYEVYDMVELQQLYEQKRAEGHEG
                     LIVKDPMCIYKRGKKSGWWKMKPENEADGIIQGLVWGTKGLANEGKVIGFEVLLESGR
                     LVNATNISRALMDEFTETVKEATLSQWGFFSPYGIGDNDACTINPYDGWACQISYMEE
                     TPDGSLRHPSFVMFRGTEDNPQEKM"
     regulatory      5420
                     /regulatory_class="terminator"
                     /note="E. coli transcription terminator TE"
     gene            5440..5592
                     /locus_tag="T7delholinF1_11"
                     /note="gene 1.4"
                     /db_xref="GeneID:1261075"
     CDS             5440..5592
                     /locus_tag="T7delholinF1_11"
                     /note="gene 1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13886.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03791"
                     /db_xref="GeneID:1261075"
                     /translation="MFKKVGKFLAALAAILTLAYILAVYPQVALVVGACYLAAVCACV
                     WSIVNW"
     regulatory      5607
                     /regulatory_class="promoter"
                     /note="T7 promoter phi1.5"
     gene            5620..5709
                     /locus_tag="T7delholinF1_12"
                     /note="gene 1.5"
                     /db_xref="GeneID:1261074"
     CDS             5620..5709
                     /locus_tag="T7delholinF1_12"
                     /note="gene 1.5"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13887.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03792"
                     /db_xref="GeneID:1261074"
                     /translation="MMYLMPLLIVIVGCLALHCSDDDMPDGHA"
     regulatory      5724
                     /regulatory_class="promoter"
                     /note="T7 promoter phi1.6"
     gene            5735..5995
                     /locus_tag="T7delholinF1_13"
                     /note="gene 1.6"
                     /db_xref="GeneID:1261076"
     CDS             5735..5995
                     /locus_tag="T7delholinF1_13"
                     /note="gene 1.6"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13888.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03793"
                     /db_xref="GeneID:1261076"
                     /translation="MFRLHYNKSVKNFTVRRADRSIVCASERRAKIPLIGNTVPLAPS
                     VHIIITRGDFEKAIDKKRPVLSVAVTRFPFVRLLLKRIKEVF"
     gene            5995..6585
                     /locus_tag="T7delholinF1_14"
                     /note="gene 1.7"
                     /db_xref="GeneID:1261060"
     CDS             5995..6585
                     /locus_tag="T7delholinF1_14"
                     /note="gene 1.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13889.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03781"
                     /db_xref="GeneID:1261060"
                     /translation="MGLLDGEAWEKENPPVQATGCIACLEKDDRYPHTCNKGANDMTE
                     REQEMIIKLIDNNEGRPDDLNGCGILCSNVPCHLCPANNDQKITLGEIRAMDPRKPHL
                     NKPEVTPTDDQPSAETIEGVTKPSHYMLFDDIEAIEVIARSMTVEQFKGYCFGNILKY
                     RLRAGKKSELAYLEKDLAKADFYKELFEKHKDKCYA"
     gene            6449..7588
                     /locus_tag="T7delholinF1_10"
                     /note="gene 1.3"
                     /db_xref="GeneID:1261055"
     gene            6578..6724
                     /locus_tag="T7delholinF1_15"
                     /note="gene 1.8"
                     /db_xref="GeneID:1261054"
     CDS             6578..6724
                     /locus_tag="T7delholinF1_15"
                     /note="not essential in T7. Other names: gp1.8"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13890.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03794"
                     /db_xref="GeneID:1261054"
                     /translation="MHNFKSTPPADSLSDDFTSCSEWCRKMWEETFDDAYIKLYELWK
                     SRGQ"
     gene            6727..6921
                     /locus_tag="T7delholinF1_16"
                     /note="gene 2"
                     /db_xref="GeneID:1261073"
     CDS             6727..6921
                     /locus_tag="T7delholinF1_16"
                     /note="T7 RNA polymerase inhibitor binds to host RNA pol
                     and suppresses its activity on a subset of promoters. gp2
                     deficient T7 display reduced DNA replication and premature
                     breakdown of replicating DNA, specifically at the left end
                     of the genome, along with the presence of empty proheads.
                     Rifampin can compensate for the missing gp2 function.
                     Other names: gp2."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of host bacterial RNA polymerase"
                     /protein_id="QWY13891.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03704"
                     /db_xref="GeneID:1261073"
                     /translation="MSNVNTGSLSVDNKKFWATVESSEHSFEVPIYAETLDEALELAE
                     WQYVPAGFEVTRVRPCVAPK"
     regulatory      6936
                     /regulatory_class="promoter"
                     /locus_tag="T7delholinF1_10"
                     /note="T7 promoter phi2.5"
     gene            6987..7685
                     /locus_tag="T7delholinF1_17"
                     /note="gene 2.5"
                     /db_xref="GeneID:1261080"
     CDS             6987..7685
                     /locus_tag="T7delholinF1_17"
                     /note="binds single-stranded DNA. In phage T7 gp2.5 is
                     essential for DNA replication and recombination. Other
                     names: gp2.5; SSB."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="QWY13892.1"
                     /db_xref="GOA:P03696"
                     /db_xref="UniProtKB/Swiss-Prot:P03696"
                     /db_xref="GeneID:1261080"
                     /translation="MAKKIFTSALGTAEPYAYIAKPDYGNEERGFGNPRGVYKVDLTI
                     PNKDPRCQRMVDEIVKCHEEAYAAAVEEYEANPPAVARGKKPLKPYEGDMPFFDNGDG
                     TTTFKFKCYASFQDKKTKETKHINLVVVDSKGKKMEDVPIIGGGSKLKVKYSLVPYKW
                     NTAVGASVKLQLESVMLVELATFGGGEDDWADEVEENGYVASGSAKASKPRDEESWDE
                     DDEESEEADEDGDF"
     gene            7686..8105
                     /locus_tag="T7delholinF1_18"
                     /note="gene 2.8"
                     /db_xref="GeneID:1261078"
     CDS             7686..8105
                     /locus_tag="T7delholinF1_18"
                     /note="gene 2.8"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13893.1"
                     /db_xref="GOA:P03795"
                     /db_xref="UniProtKB/Swiss-Prot:P03795"
                     /db_xref="GeneID:1261078"
                     /translation="MELREKILERIKVTSSGCWEWQGATNNKGYGQVWCSNTGKVVYC
                     HRVMSNAPKGSTVLHSCDNPLCCNPEHLSIGTPKENSTDMVNKGRSHKGYKLSDEDVM
                     AIMESSESNVSLARTYGVSQQTICDIRKGRRHGRLRR"
     gene            8086..8535
                     /locus_tag="T7delholinF1_19"
                     /note="gene 3"
                     /db_xref="GeneID:1261079"
     CDS             8086..8535
                     /locus_tag="T7delholinF1_19"
                     /note="T7 endonuclease I is a Holliday junction resolvase
                     encoded by T7 gene 3. Mutants in gene 3 are defective in
                     recombination and accumulate branched DNA. Endonuclease I
                     may also play a role in the degradation of the host genome
                     following infection with T7."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease I"
                     /protein_id="QWY13894.1"
                     /db_xref="GOA:P00641"
                     /db_xref="UniProtKB/Swiss-Prot:P00641"
                     /db_xref="GeneID:1261079"
                     /translation="MAGYGAKGIRKVGAFRSGLEDKVSKQLESKGIKFEYEEWKVPYV
                     IPASNHTYTPDFLLPNGIFVETKGLWESDDRKKHLLIREQHPELDIRIVFSSSRTKLY
                     KGSPTSYGEFCEKHGIKFADKLIPAEWIKEPKKEVPFDRLKRKGGKK"
     gene            8535..8990
                     /locus_tag="T7delholinF1_20"
                     /note="gene 3.5"
                     /db_xref="GeneID:1261077"
     CDS             8535..8990
                     /locus_tag="T7delholinF1_20"
                     /note="T7 lysozyme hydrolyzes an amide bond in the host
                     cell wall following its release from the cytoplasm. In
                     addition, T7 lysozyme inhibits T7 RNA polymerase
                     initiation. This inhibition is greater for class II
                     promoters than class III promoters and therefore may aid
                     in temporal regulation of transcription and the switch to
                     particle assembly. In T7, lysozyme, unlike the T7 holin,
                     is expressed with and lies in the same region as the
                     replication genes. Lack of gp3.5 reduces replication and
                     burst size and delays, but does not completely prevent
                     lysis. Mutations in the muralytic domain of gene 16, an
                     inner capsid protein, can partially compensate for a
                     deletion of gp3.5. Other names: gp3.5; amidase;
                     N-acetylmuramoyl-L-alanine amidase"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="QWY13895.1"
                     /db_xref="GOA:P00806"
                     /db_xref="UniProtKB/Swiss-Prot:P00806"
                     /db_xref="GeneID:1261077"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLD
                     VGYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQ
                     SLRSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
     gene            9041..10741
                     /locus_tag="T7delholinF1_22"
                     /note="gene 4A"
                     /db_xref="GeneID:1261046"
     CDS             9041..10741
                     /locus_tag="T7delholinF1_22"
                     /note="gene 4A, primase/helicase [14,15]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase/helicase"
                     /protein_id="QWY13896.1"
                     /db_xref="GOA:P03692"
                     /db_xref="UniProtKB/Swiss-Prot:P03692"
                     /db_xref="GeneID:1261046"
                     /translation="MDNSHDSDSVFLYHIPCDNCGSSDGNSLFSDGHTFCYVCEKWTA
                     GNEDTKERASKRKPSGGKPMTYNVWNFGESNGRYSALTARGISKETCQKAGYWIAKVD
                     GVMYQVADYRDQNGNIVSQKVRDKDKNFKTTGSHKSDALFGKHLWNGGKKIVVTEGEI
                     DMLTVMELQDCKYPVVSLGHGASAAKKTCAANYEYFDQFEQIILMFDMDEAGRKAVEE
                     AAQVLPAGKVRVAVLPCKDANECHLNGHDREIMEQVWNAGPWIPDGVVSALSLRERIR
                     EHLSSEESVGLLFSGCTGINDKTLGARGGEVIMVTSGSGMGKSTFVRQQALQWGTAMG
                     KKVGLAMLEESVEETAEDLIGLHNRVRLRQSDSLKREIIENGKFDQWFDELFGNDTFH
                     LYDSFAEAETDRLLAKLAYMRSGLGCDVIILDHISIVVSASGESDERKMIDNLMTKLK
                     GFAKSTGVVLVVICHLKNPDKGKAHEEGRPVSITDLRGSGALRQLSDTIIALERNQQG
                     DMPNLVLVRILKCRFTGDTGIAGYMEYNKETGWLEPSSYSGEEESHSESTDWSNDTDF
                     "
     gene            9111..9233
                     /locus_tag="T7delholinF1_23"
                     /note="gene 4.1"
                     /db_xref="GeneID:1261047"
     CDS             9111..9233
                     /locus_tag="T7delholinF1_23"
                     /note="gene 4.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13897.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03782"
                     /db_xref="GeneID:1261047"
                     /translation="MGTRCSLTDTRSATYARSGLLVMKTLKRGLQNGNPQEVNQ"
     gene            9230..10741
                     /locus_tag="T7delholinF1_24"
                     /note="gene 4B"
                     /db_xref="GeneID:1261048"
     CDS             9230..10741
                     /locus_tag="T7delholinF1_24"
                     /note="gene 4B/helicase [14,15]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="QWY13898.1"
                     /db_xref="GOA:P03692"
                     /db_xref="UniProtKB/Swiss-Prot:P03692"
                     /db_xref="GeneID:1261048"
                     /translation="MTYNVWNFGESNGRYSALTARGISKETCQKAGYWIAKVDGVMYQ
                     VADYRDQNGNIVSQKVRDKDKNFKTTGSHKSDALFGKHLWNGGKKIVVTEGEIDMLTV
                     MELQDCKYPVVSLGHGASAAKKTCAANYEYFDQFEQIILMFDMDEAGRKAVEEAAQVL
                     PAGKVRVAVLPCKDANECHLNGHDREIMEQVWNAGPWIPDGVVSALSLRERIREHLSS
                     EESVGLLFSGCTGINDKTLGARGGEVIMVTSGSGMGKSTFVRQQALQWGTAMGKKVGL
                     AMLEESVEETAEDLIGLHNRVRLRQSDSLKREIIENGKFDQWFDELFGNDTFHLYDSF
                     AEAETDRLLAKLAYMRSGLGCDVIILDHISIVVSASGESDERKMIDNLMTKLKGFAKS
                     TGVVLVVICHLKNPDKGKAHEEGRPVSITDLRGSGALRQLSDTIIALERNQQGDMPNL
                     VLVRILKCRFTGDTGIAGYMEYNKETGWLEPSSYSGEEESHSESTDWSNDTDF"
     regulatory      10147
                     /regulatory_class="promoter"
                     /locus_tag="T7delholinF1_24"
                     /note="T7 promoter phi4c"
     gene            10464..10802
                     /locus_tag="T7delholinF1_25"
                     /note="gene 4.2"
                     /db_xref="GeneID:1261021"
     CDS             10464..10802
                     /locus_tag="T7delholinF1_25"
                     /note="gene 4.2"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13899.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03783"
                     /db_xref="GeneID:1261021"
                     /translation="MRKVAPFLLLTYVVLAHYANYLILLLPLSVISKAICLTLSSFVF
                     SSAALLVILVSLATWNTTRKPDGLNHQVTQGKKSHTQSQQTGPTTLTSDRILDDFPDD
                     YEKFRWRVPF"
     regulatory      10817
                     /regulatory_class="promoter"
                     /note="T7 promoter phi4.3"
     gene            10828..11040
                     /locus_tag="T7delholinF1_26"
                     /note="gene 4.3"
                     /db_xref="GeneID:1261069"
     CDS             10828..11040
                     /locus_tag="T7delholinF1_26"
                     /note="not essential in T7; Other names: gp4.3."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13900.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03784"
                     /db_xref="GeneID:1261069"
                     /translation="MFKLIKKLGQLLVRMYNVEAKRLNDEARKEATQSRALAIRSNEL
                     ADSASTKVTEAARVANQAQQLSKFFE"
     gene            11060..11329
                     /locus_tag="T7delholinF1_27"
                     /note="gene 4.5"
                     /db_xref="GeneID:1261059"
     CDS             11060..11329
                     /locus_tag="T7delholinF1_27"
                     /note="not essential in T7. Other names: gp4.5."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13901.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03785"
                     /db_xref="GeneID:1261059"
                     /translation="MSNVAETIRLSDTADQWNRRVHINVRNGKATMVYRWKDSKSSKN
                     HTQRMTLTDEQALRLVNALTKAAVTAIHEAGRVNEAMAILDKIDN"
     misc_structure  11368
                     /note="RNase III site R4.7"
     regulatory      11391
                     /regulatory_class="promoter"
                     /note="T7 promoter phi4.7"
     gene            11403..11810
                     /locus_tag="T7delholinF1_28"
                     /note="gene 4.7"
                     /db_xref="GeneID:1261043"
     CDS             11403..11810
                     /locus_tag="T7delholinF1_28"
                     /note="gene 4.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13902.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03786"
                     /db_xref="GeneID:1261043"
                     /translation="MRDPKVIQAEIAKLEAELEDVKYHEAKTRSAVHILKNLGWTWTR
                     QTGWKKPEVTKLSHKVFDKDTMTHIKAGDWVKVDMGVVGGYGYVRSVSGKYAQVSYIT
                     GVTPRGAIVADKTNMIHTGFLTVVSYEEIVKSR"
     gene            11829..13943
                     /locus_tag="T7delholinF1_29"
                     /note="gene 5"
                     /db_xref="GeneID:1261044"
     CDS             11829..13943
                     /locus_tag="T7delholinF1_29"
                     /note="gene 5"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="QWY13903.1"
                     /db_xref="GOA:P00581"
                     /db_xref="UniProtKB/Swiss-Prot:P00581"
                     /db_xref="GeneID:1261044"
                     /translation="MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLD
                     ALEAEVARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHSNL
                     KDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEW
                     WNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTFWSESLEAVDIEH
                     RAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRKLTETFGSWYQPKGGTEMFCH
                     PRTGKPLPKYPRIKTPKVGGIFKKPKNKAQREGREPCELDTREYVAGAPYTPVEHVVF
                     NPSSRDHIQKKLQEAGWVPTKYTDKGAPVVDDEVLEGVRVDDPEKQAAIDLIKEYLMI
                     QKRIGQSAEGDKAWLRYVAEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQ
                     CRAAFGAEHHLDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTK
                     NQIAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALR
                     ESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKL
                     WIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETAQEAMRWVGD
                     HWNFRCLLDTEGKMGPNWAICH"
     gene            13959..14315
                     /locus_tag="T7delholinF1_30"
                     /note="gene 5.3"
                     /db_xref="GeneID:1261045"
     CDS             13959..14315
                     /locus_tag="T7delholinF1_30"
                     /note="gene 5.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13904.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03798"
                     /db_xref="GeneID:1261045"
                     /translation="MNERHLTGAASEMLVAYKFTKAGYTVYYPMLTQSKEDLVVCKDG
                     KFSKVQVKTATTVQTNTGDAKQVRLGGCGRSEYKDGDFDILAVVVDEDVLIFTWDEVK
                     GKTSMCVGKRNKGIKL"
     gene            14327..14835
                     /locus_tag="T7delholinF1_31"
                     /note="possible gene 5.5-5.7"
                     /db_xref="GeneID:1261041"
     CDS             join(14327..14623,14623..14835)
                     /locus_tag="T7delholinF1_31"
                     /ribosomal_slippage
                     /note="possible gene 5.5-5.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13905.1"
                     /db_xref="GOA:P03787"
                     /db_xref="UniProtKB/Swiss-Prot:P03787"
                     /db_xref="GeneID:1261041"
                     /translation="MAMTKKFKVSFDVTAKMSSDVQAVLEKDMLHLCKQVGSGAIVPN
                     GKQKEMIVQFLTHGMEGLMTFVVRTSFREAIKDMHEEYADKDSFKQSPATVREVFLMS
                     DYLKVLQAIKSCPKTFQSNYVRNNASLVAEAASRGHISCLTTSGRNGGAWEITASGTR
                     FLKRMGGCV"
     gene            14327..14626
                     /locus_tag="T7delholinF1_32"
                     /note="gene 5.5"
                     /db_xref="GeneID:1261038"
     CDS             14327..14626
                     /locus_tag="T7delholinF1_32"
                     /note="in Enterobacteria phage T7, gp5.5 abolishes E. coli
                     nucleoid protein H-NS-mediated inhibition of transcription
                     by T7 RNA polymerases in vitro. Not essential, but mutants
                     have lower burst size. Mutants in this gene are not
                     capable of replicating in phage lambda lysogens. Other
                     names: gp5.5"
                     /codon_start=1
                     /transl_table=11
                     /product="host protein H-NS-interacting protein"
                     /protein_id="QWY13906.1"
                     /db_xref="GOA:P03787"
                     /db_xref="UniProtKB/Swiss-Prot:P03787"
                     /db_xref="GeneID:1261038"
                     /translation="MAMTKKFKVSFDVTAKMSSDVQAVLEKDMLHLCKQVGSGAIVPN
                     GKQKEMIVQFLTHGMEGLMTFVVRTSFREAIKDMHEEYADKDSFKQSPATVREVF"
     gene            14626..14835
                     /locus_tag="T7delholinF1_33"
                     /note="gene 5.7"
                     /db_xref="GeneID:1261040"
     CDS             14626..14835
                     /locus_tag="T7delholinF1_33"
                     /note="gene 5.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13907.1"
                     /db_xref="GOA:P03787"
                     /db_xref="UniProtKB/Swiss-Prot:P03787"
                     /db_xref="GeneID:1261040"
                     /translation="MSDYLKVLQAIKSCPKTFQSNYVRNNASLVAEAASRGHISCLTT
                     SGRNGGAWEITASGTRFLKRMGGCV"
     gene            14835..14993
                     /locus_tag="T7delholinF1_34"
                     /note="gene 5.9"
                     /db_xref="GeneID:1261037"
     CDS             14835..14993
                     /locus_tag="T7delholinF1_34"
                     /note="not essential. Other names: gp5.9; exonuclease V
                     inhibitor"
                     /codon_start=1
                     /transl_table=11
                     /product="host recBCD nuclease inhibitor"
                     /protein_id="QWY13908.1"
                     /db_xref="UniProtKB/Swiss-Prot:P20406"
                     /db_xref="GeneID:1261037"
                     /translation="MSRDLVTIPRDVWNDIQGYIDSLERENDSLKNQLMEADEYVAEL
                     EEKLNGTS"
     gene            14980..15882
                     /locus_tag="T7delholinF1_35"
                     /note="gene 6"
                     /db_xref="GeneID:1261052"
     CDS             14980..15882
                     /locus_tag="T7delholinF1_35"
                     /note="The T7 exonuclease encoded by gene 6 is required
                     for (a) recombination and (b) for the degradation of host
                     chromosomal DNA. The latter process provides nucleotides
                     for phage DNA replication. Both processes are carried out
                     together with the T7 gene 3-encoded endonuclease/Holliday
                     junction resolvase. In addition, the exonuclease also
                     functions as an RNase H that removes RNA primers during
                     DNA replication and promotes concatemer formation."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="QWY13909.1"
                     /db_xref="GOA:P00638"
                     /db_xref="UniProtKB/Swiss-Prot:P00638"
                     /db_xref="GeneID:1261052"
                     /translation="MALLDLKQFYELREGCDDKGILVMDGDWLVFQAMSAAEFDASWE
                     EEIWHRCCDHAKARQILEDSIKSYETRKKAWAGAPIVLAFTDSVNWRKELVDPNYKAN
                     RKAVKKPVGYFEFLDALFEREEFYCIREPMLEGDDVMGVIASNPSAFGARKAVIISCD
                     KDFKTIPNCDFLWCTTGNILTQTEESADWWHLFQTIKGDITDGYSGIAGWGDTAEDFL
                     NNPFITEPKTSVLKSGKNKGQEVTKWVKRDPEPHETLWDCIKSIGAKAGMTEEDIIKQ
                     GQMARILRFNEYNFIDKEIYLWRP"
     gene            15870..15983
                     /locus_tag="T7delholinF1_36"
                     /note="gene 6.3"
                     /db_xref="GeneID:1261058"
     CDS             15870..15983
                     /locus_tag="T7delholinF1_36"
                     /note="gene 6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13910.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03799"
                     /db_xref="GeneID:1261058"
                     /translation="METVAYIGLDLCVLGVCLISWGLWDLARIIKSLHDTK"
     regulatory      16021
                     /regulatory_class="promoter"
                     /note="T7 promoter phi6.5"
     misc_structure  16039
                     /note="RNase III site R6.5"
     gene            16081..16335
                     /locus_tag="T7delholinF1_37"
                     /note="gene 6.5"
                     /db_xref="GeneID:1261036"
     CDS             16081..16335
                     /locus_tag="T7delholinF1_37"
                     /note="gene 6.5"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2717 domain-containing protein"
                     /protein_id="QWY13911.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03800"
                     /db_xref="GeneID:1261036"
                     /translation="MLTPINQLLKNPNDIPDVPRATAEYLQVRFNYAYLEASGHIGLM
                     RANGCSEAHILGFIQGLQYASNVIDEIELRKEQLRDDGED"
     gene            16340..16606
                     /locus_tag="T7delholinF1_38"
                     /note="gene 6.7"
                     /db_xref="GeneID:1261039"
     CDS             16340..16606
                     /locus_tag="T7delholinF1_38"
                     /note="may be involved in virion morphogenesis and is
                     injected from virion into host cell. Other names: gp6.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13912.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03801"
                     /db_xref="GeneID:1261039"
                     /translation="MCFSPKIKTPKMDTNQIRAVEPAPLTQEVSSVEFGGSSDETDTE
                     GTEVSGRKGLKVERDDSVAKSKASGNGSARMKSSIRKSAFGGKK"
     gene            16606..17007
                     /locus_tag="T7delholinF1_39"
                     /note="gene 7"
                     /db_xref="GeneID:1261056"
     CDS             16606..17007
                     /locus_tag="T7delholinF1_39"
                     /note="gene 7, host range"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13913.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03750"
                     /db_xref="GeneID:1261056"
                     /translation="MSEFTCMEAKSRFRAIRWTVEHLGLPKGFEGHFVGYSLYVDEVM
                     DMSGCREEYILDSTGKHVAYFAWCVSCDIHHKGDILDVTSVVINPEADSKGLQRFLAK
                     RFKYLAELHDCDWVSRCKHEGETMRVYFKEV"
     gene            17011..17310
                     /locus_tag="T7delholinF1_40"
                     /note="gene 7.3"
                     /db_xref="GeneID:1261035"
     CDS             17011..17310
                     /locus_tag="T7delholinF1_40"
                     /note="required for virion infectivity but not
                     morphogenesis. In T7, gp 7.3 appears to be required for
                     the assembly of tail fibers on capsids. Other names:
                     gp7.3"
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="QWY13914.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03751"
                     /db_xref="GeneID:1261035"
                     /translation="MGKKVKKAVKKVTKSVKKVVKEGARPVKQVAGGLAGLAGGTGEA
                     QMVEVPQAAAQIVDVPEKEVSTEDEAQTESGRKKARAGGKKSLSVARSSGGGINI"
     gene            17324..17716
                     /locus_tag="T7delholinF1_41"
                     /note="gene 7.7"
                     /db_xref="GeneID:1261028"
     CDS             17324..17716
                     /locus_tag="T7delholinF1_41"
                     /note="gene 7.7"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13915.1"
                     /db_xref="GOA:P03796"
                     /db_xref="UniProtKB/Swiss-Prot:P03796"
                     /db_xref="GeneID:1261028"
                     /translation="MEDCIEWTGDVNSKGYGRKWVNGKLVTPHRHIYEETYGPVPTGI
                     VVMHICDNPRCYNIKHLTLGTPKDNSEDMVTKGRQAKGEELSKKLTESDVLAIRSSTL
                     SHRSLGELYGVSQSTITRILQRKTWRHI"
     gene            17716..19326
                     /locus_tag="T7delholinF1_42"
                     /note="gene 8"
                     /db_xref="GeneID:1261033"
     CDS             17716..19326
                     /locus_tag="T7delholinF1_42"
                     /note="gene 8"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="QWY13916.1"
                     /db_xref="GOA:P03728"
                     /db_xref="UniProtKB/Swiss-Prot:P03728"
                     /db_xref="GeneID:1261033"
                     /translation="MAEKRTGLAEDGAKSVYERLKNDRAPYETRAQNCAQYTIPSLFP
                     KDSDNASTDYQTPWQAVGARGLNNLASKLMLALFPMQTWMRLTISEYEAKQLLSDPDG
                     LAKVDEGLSMVERIIMNYIESNSYRVTLFEALKQLVVAGNVLLYLPEPEGSNYNPMKL
                     YRLSSYVVQRDAFGNVLQMVTRDQIAFGALPEDIRKAVEGQGGEKKADETIDVYTHIY
                     LDEDSGEYLRYEEVEGMEVQGSDGTYPKEACPYIPIRMVRLDGESYGRSYIEEYLGDL
                     RSLENLQEAIVKMSMISSKVIGLVNPAGITQPRRLTKAQTGDFVTGRPEDISFLQLEK
                     QADFTVAKAVSDAIEARLSFAFMLNSAVQRTGERVTAEEIRYVASELEDTLGGVYSIL
                     SQELQLPLVRVLLKQLQATQQIPELPKEAVEPTISTGLEAIGRGQDLDKLERCVTAWA
                     ALAPMRDDPDINLAMIKLRIANAIGIDTSGILLTEEQKQQKMAQQSMQMGMDNGAAAL
                     AQGMAAQATASPEAMAAAADSVGLQPGI"
     regulatory      19341
                     /regulatory_class="promoter"
                     /note="T7 promoter phi9"
     gene            19426..20349
                     /locus_tag="T7delholinF1_43"
                     /note="gene 9"
                     /db_xref="GeneID:1261027"
     CDS             19426..20349
                     /locus_tag="T7delholinF1_43"
                     /note="Phage T7-like scaffolding protein. The protein is
                     encoded by gene 9 in T7 (gp9) and is required for the
                     formation of pro-capsids."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid assembly protein"
                     /protein_id="QWY13917.1"
                     /db_xref="GOA:P03716"
                     /db_xref="UniProtKB/Swiss-Prot:P03716"
                     /db_xref="GeneID:1261027"
                     /translation="MAESNADVYASFGVNSAVMSGGSVEEHEQNMLALDVAARDGDDA
                     IELASDEVETERDLYDNSDPFGQEDDEGRIQVRIGDGSEPTDVDTGEEGVEGTEGSEE
                     FTPLGETPEELVAASEQLGEHEEGFQEMINIAAERGMSVETIEAIQREYEENEELSAE
                     SYAKLAEIGYTKAFIDSYIRGQEALVEQYVNSVIEYAGGRERFDALYNHLETHNPEAA
                     QSLDNALTNRDLATVKAIINLAGESRAKAFGRKPTRSVTNRAIPAKPQATKREGFADR
                     SEMIKAMSDPRYRTDANYRRQVEQKVIDSNF"
     regulatory      20380
                     /regulatory_class="promoter"
                     /note="T7 promoter phi10"
     gene            20443..21537
                     /locus_tag="T7delholinF1_44"
                     /note="gene 10B"
                     /db_xref="GeneID:1261029"
     CDS             20443..21537
                     /locus_tag="T7delholinF1_44"
                     /note="minor capsid protein. A minor capsid protein
                     (gp10B) is produced from gene 10 by a -1 frameshift
                     towards the end of 10A. A multiple cloning site was
                     engineered at the frameshift site, resulting in a surface
                     displaying function."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="QWY13918.1"
                     /db_xref="GOA:P19727"
                     /db_xref="UniProtKB/Swiss-Prot:P19727"
                     /db_xref="GeneID:1261029"
                     /translation="MASMTGGQQMGTNQGKGVVAAGDKLALFLKVFGGEVLTAFARTS
                     VTTSRHMVRSISSGKSAQFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTA
                     DVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLG
                     TATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAIL
                     AALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTAREGTTGQKHVFPANK
                     GEGNVKVAKDNVIGLFMHRSAVGTVKLRDLALERARRANFQADQIIAKYAMGHGGLRP
                     EAAGAVVFQSGVMLGDPNSSSVDKLAAALE"
     regulatory      21584
                     /regulatory_class="terminator"
                     /note="T7 transcription terminator Tphi"
     gene            21602..22192
                     /locus_tag="T7delholinF1_46"
                     /note="gene 11"
                     /db_xref="GeneID:1261030"
     CDS             21602..22192
                     /locus_tag="T7delholinF1_46"
                     /note="Tail tubular proteins A and B are required for
                     assembly of tails of T7-like phages."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tubular protein A"
                     /protein_id="QWY13919.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03746"
                     /db_xref="GeneID:1261030"
                     /translation="MRSYDMNVETAAELSAVNDILASIGEPPVSTLEGDANADAANAR
                     RILNKINRQIQSRGWTFNIEEGITLLPDVYSNLIVYSDDYLSLMSTSGQSIYVNRGGY
                     VYDRTSQSDRFDSGITVNIIRLRDYDEMPECFRYWIVTKASRQFNNRFFGAPEVEGVL
                     QEEEDEARRLCMEYEMDYGGYNMLDGDAFTSGLLTR"
     gene            22216..24600
                     /locus_tag="T7delholinF1_47"
                     /note="gene 12"
                     /db_xref="GeneID:1261024"
     CDS             22216..24600
                     /locus_tag="T7delholinF1_47"
                     /note="gene 12"
                     /codon_start=1
                     /transl_table=11
                     /product="tail tubular protein B"
                     /protein_id="QWY13920.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03747"
                     /db_xref="GeneID:1261024"
                     /translation="MALISQSIKNLKGGISQQPDILRYPDQGSRQVNGWSSETEGLQK
                     RPPLVFLNTLGDNGALGQAPYIHLINRDEHEQYYAVFTGSGIRVFDLSGNEKQVRYPN
                     GSNYIKTANPRNDLRMVTVADYTFIVNRNVVAQKNTKSVNLPNYNPNQDGLINVRGGQ
                     YGRELIVHINGKDVAKYKIPDGSQPEHVNNTDAQWLAEELAKQMRTNLSDWTVNVGQG
                     FIHVTAPSGQQIDSFTTKDGYADQLINPVTHYAQSFSKLPPNAPNGYMVKIVGDASKS
                     ADQYYVRYDAERKVWTETLGWNTEDQVLWETMPHALVRAADGNFDFKWLEWSPKSCGD
                     VDTNPWPSFVGSSINDVFFFRNRLGFLSGENIILSRTAKYFNFYPASIANLSDDDPID
                     VAVSTNRIAILKYAVPFSEELLIWSDEAQFVLTASGTLTSKSVELNLTTQFDVQDRAR
                     PFGIGRNVYFASPRSSFTSIHRYYAVQDVSSVKNAEDITSHVPNYIPNGVFSICGSGT
                     ENFCSVLSHGDPSKIFMYKFLYLNEELRQQSWSHWDFGENVQVLACQSISSDMYVILR
                     NEFNTFLARISFTKNAIDLQGEPYRAFMDMKIRYTIPSGTYNDDTFTTSIHIPTIYGA
                     NFGRGKITVLEPDGKITVFEQPTAGWNSDPWLRLSGNLEGRMVYIGFNINFVYEFSKF
                     LIKQTADDGSTSTEDIGRLQLRRAWVNYENSGTFDIYVENQSSNWKYTMAGARLGSNT
                     LRAGRLNLGTGQYRFPVVGNAKFNTVYILSDETTPLNIIRCGWEGNYLRRSSGI"
     regulatory      24648
                     /regulatory_class="promoter"
                     /note="T7 promoter phi13"
     misc_structure  24655
                     /note="possible RNase III site R13"
     gene            24681..25097
                     /locus_tag="T7delholinF1_48"
                     /note="gene 13"
                     /db_xref="GeneID:1261025"
     CDS             24681..25097
                     /locus_tag="T7delholinF1_48"
                     /note="gene 13"
                     /codon_start=1
                     /transl_table=11
                     /product="internal virion protein A"
                     /protein_id="QWY13921.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03723"
                     /db_xref="GeneID:1261025"
                     /translation="MMTIRPTKSTDFEVFTPAHHDILEAKAAGIEPSFPDASECVTLS
                     LYGFPLAIGGNCGDQCWFVTSDQVWRLSGKAKRKFRKLIMEYRDKMLEKYDTLWNYVW
                     VGNTSHIRFLKTIGAVFHEEYTRDGQFQLFTITKGG"
     gene            25102..25692
                     /locus_tag="T7delholinF1_49"
                     /note="gene 14"
                     /db_xref="GeneID:1261032"
     CDS             25102..25692
                     /locus_tag="T7delholinF1_49"
                     /note="Approximately 12 copies of the internal virion
                     protein B encoded in phage T7 by gene 14 are part of the
                     internal core of the T7 virion. Along with gp16 and gp15,
                     the other internal core proteins, gp14 is ejected from the
                     phage head and forms part of a putative channel that spans
                     the entire host cell envelope and allows entry of DNA.
                     gp14 appears to localize to the outer host membrane after
                     ejection. Other names: gp14"
                     /codon_start=1
                     /transl_table=11
                     /product="internal virion protein B"
                     /protein_id="QWY13922.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03724"
                     /db_xref="GeneID:1261032"
                     /translation="MCWAAAIPIAISGAQAISGQNAQAKMIAAQTAAGRRQAMEIMRQ
                     TNIQNADLSLQARSKLEEASAELTSQNMQKVQAIGSIRAAIGESMLEGSSMDRIKRVT
                     EGQFIREANMVTENYRRDYQAIFAQQLGGTQSAASQIDEIYKSEQKQKSKLQMVLDPL
                     AIMGSSAASAYASGAFDSKSTTKAPIVAAKGTKTGR"
     gene            25699..27942
                     /locus_tag="T7delholinF1_50"
                     /note="gene 15"
                     /db_xref="GeneID:1261034"
     CDS             25699..27942
                     /locus_tag="T7delholinF1_50"
                     /note="Approximately 12 copies of the internal virion
                     protein C encoded by phage T7 gene 15 (gp15) are part of
                     the internal core of the T7 virion. Along with gp14 and
                     gp16, the other internal core proteins, gp15 is ejected
                     from the phage head and forms part of a putative channel
                     that spans the entire host cell envelope and allows entry
                     of DNA."
                     /codon_start=1
                     /transl_table=11
                     /product="internal virion protein C"
                     /protein_id="QWY13923.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03725"
                     /db_xref="GeneID:1261034"
                     /translation="MSKIESALQAAQPGLSRLRGGAGGMGYRAATTQAEQPRSSLLDT
                     IGRFAKAGADMYTAKEQRARDLADERSNEIIRKLTPEQRREALNNGTLLYQDDPYAME
                     ALRVKTGRNAAYLVDDDVMQKIKEGVFRTREEMEEYRHSRLQEGAKVYAEQFGIDPED
                     VDYQRGFNGDITERNISLYGAHDNFLSQQAQKGAIMNSRVELNGVLQDPDMLRRPDSA
                     DFFEKYIDNGLVTGAIPSDAQATQLISQAFSDASSRAGGADFLMRVGDKKVTLNGATT
                     TYRELIGEEQWNALMVTAQRSQFETDAKLNEQYRLKINSALNQEDPRTAWEMLQGIKA
                     ELDKVQPDEQMTPQREWLISAQEQVQNQMNAWTKAQAKALDDSMKSMNKLDVIDKQFQ
                     KRINGEWVSTDFKDMPVNENTGEFKHSDMVNYANKKLAEIDSMDIPDGAKDAMKLKYL
                     QADSKDGAFRTAIGTMVTDAGQEWSAAVINGKLPERTPAMDALRRIRNADPQLIAALY
                     PDQAELFLTMDMMDKQGIDPQVILDADRLTVKRSKEQRFEDDKAFESALNASKAPEIA
                     RMPASLRESARKIYDSVKYRSGNESMAMEQMTKFLKESTYTFTGDDVDGDTVGVIPKN
                     MMQVNSDPKSWEQGRDILEEARKGIIASNPWITNKQLTMYSQGDSIYLMDTTGQVRVR
                     YDKELLSKVWSENQKKLEEKAREKALADVNKRAPIVAATKAREAAAKRVREKRKQTPK
                     FIYGRKE"
     gene            27969..31925
                     /locus_tag="T7delholinF1_51"
                     /note="gene 16"
                     /db_xref="GeneID:1261031"
     CDS             27969..31925
                     /locus_tag="T7delholinF1_51"
                     /note="Approximately 3 copies of the internal virion
                     protein D encoded by phage T7 gene 16 (gp16) are part of
                     the internal core of the T7 virion. Along with gp14 and
                     gp15, the other internal core proteins, gp16 is ejected
                     from the phage head and forms part of a putative channel
                     that spans the entire host cell envelope and allows entry
                     of DNA. The N-terminus has similarity to a lytic
                     transglycosylase and may help form a channel for phage DNA
                     translocation through the crosslinked peptidoglycan layer
                     of the host envelope."
                     /codon_start=1
                     /transl_table=11
                     /product="internal virion protein D"
                     /protein_id="QWY13924.1"
                     /db_xref="GOA:P03726"
                     /db_xref="UniProtKB/Swiss-Prot:P03726"
                     /db_xref="GeneID:1261031"
                     /translation="MDKYDKNVPSDYDGLFQKAADANGVSYDLLRKVAWTESRFVPTA
                     KSKTGPLGMMQFTKATAKALGLRVTDGPDDDRLNPELAINAAAKQLAGLVGKFDGDEL
                     KAALAYNQGEGRLGNPQLEAYSKGDFASISEEGRNYMRNLLDVAKSPMAGQLETFGGI
                     TPKGKGIPAEVGLAGIGHKQKVTQELPESTSFDVKGIEQEATAKPFAKDFWETHGETL
                     DEYNSRSTFFGFKNAAEAELSNSVAGMAFRAGRLDNGFDVFKDTITPTRWNSHIWTPE
                     ELEKIRTEVKNPAYINVVTGGSPENLDDLIKLANENFENDSRAAEAGLGAKLSAGIIG
                     AGVDPLSYVPMVGVTGKGFKLINKALVVGAESAALNVASEGLRTSVAGGDADYAGAAL
                     GGFVFGAGMSAISDAVAAGLKRSKPEAEFDNEFIGPMMRLEARETARNANSADLSRMN
                     TENMKFEGEHNGVPYEDLPTERGAVVLHDGSVLSASNPINPKTLKEFSEVDPEKAARG
                     IKLAGFTEIGLKTLGSDDADIRRVAIDLVRSPTGMQSGASGKFGATASDIHERLHGTD
                     QRTYNDLYKAMSDAMKDPEFSTGGAKMSREETRYTIYRRAALAIERPELQKALTPSER
                     IVMDIIKRHFDTKRELMENPAIFGNTKAVSIFPESRHKGTYVPHVYDRHAKALMIQRY
                     GAEGLQEGIARSWMNSYVSRPEVKARVDEMLKELHGVKEVTPEMVEKYAMDKAYGISH
                     SDQFTNSSIIEENIEGLVGIENNSFLEARNLFDSDLSITMPDGQQFSVNDLRDFDMFR
                     IMPAYDRRVNGDIAIMGSTGKTTKELKDEILALKAKAEGDGKKTGEVHALMDTVKILT
                     GRARRNQDTVWETSLRAINDLGFFAKNAYMGAQNITEIAGMIVTGNVRALGHGIPILR
                     DTLYKSKPVSAKELKELHASLFGKEVDQLIRPKRADIVQRLREATDTGPAVANIVGTL
                     KYSTQELAARSPWTKLLNGTTNYLLDAARQGMLGDVISATLTGKTTRWEKEGFLRGAS
                     VTPEQMAGIKSLIKEHMVRGEDGKFTVKDKQAFSMDPRAMDLWRLADKVADEAMLRPH
                     KVSLQDSHAFGALGKMVMQFKSFTIKSLNSKFLRTFYDGYKNNRAIDAALSIITSMGL
                     AGGFYAMAAHVKAYALPKEKRKEYLERALDPTMIAHAALSRSSQLGAPLAMVDLVGGV
                     LGFESSKMARSTILPKDTVKERDPNKPYTSREVMGAMGSNLLEQMPSAGFVANVGATL
                     MNAAGVVNSPNKATEQDFMTGLMNSTKELVPNDPLTQQLVLKIYEANGVNLRERRK"
     regulatory      31940
                     /regulatory_class="promoter"
                     /note="T7 promoter phi17"
     gene            31998..33659
                     /locus_tag="T7delholinF1_52"
                     /note="gene 17"
                     /db_xref="GeneID:1261023"
     CDS             31998..33659
                     /locus_tag="T7delholinF1_52"
                     /note="in phages T7 and T3 trimers of gp17 form each of
                     the 6 kinked tail fibers. Other names: gp17."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="QWY13925.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03748"
                     /db_xref="GeneID:1261023"
                     /translation="MANVIKTVLTYQLDGSNRDFNIPFEYLARKFVVVTLIGVDRKVL
                     TINTDYRFATRTTISLTKAWGPADGYTTIELRRVTSTTDRLVDFTDGSILRAYDLNVA
                     QIQTMHVAEEARDLTTDTIGVNNDGHLDARGRRIVNLANAVDDRDAVPFGQLKTMNQN
                     SWQARNEALQFRNEAETFRNQAEGFKNESSTNATNTKQWRDETKGFRDEAKRFKNTAG
                     QYATSAGNSASAAHQSEVNAENSATASANSAHLAEQQADRAEREADKLENYNGLAGAI
                     DKVDGTNVYWKGNIHANGRLYMTTNGFDCGQYQQFFGGVTNRYSVMEWGDENGWLMYV
                     QRREWTTAIGGNIQLVVNGQIITQGGAMTGQLKLQNGHVLQLESASDKAHYILSKDGN
                     RNNWYIGRGSDNNNDCTFHSYVHGTTLTLKQDYAVVNKHFHVGQAVVATDGNIQGTKW
                     GGKWLDAYLRDSFVAKSKAWTQVWSGSAGGGVSVTVSQDLRFRNIWIKCANNSWNFFR
                     TGPDGIYFIASDGGWLRFQIHSNGLGFKNIADSRSVPNAIMVENE"
     gene            33718..33987
                     /locus_tag="T7delholinF1_54"
                     /note="gene 18"
                     /db_xref="GeneID:1261042"
     CDS             33718..33987
                     /locus_tag="T7delholinF1_54"
                     /note="involved in the packaging of genome monomers into a
                     procapsid using head-to-tail concatemers of genomes. other
                     names: DNA packaging protein A; DNA maturation protein A;
                     terminase, small subunit"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA packaging protein, small subunit"
                     /protein_id="QWY13926.1"
                     /db_xref="GOA:P03693"
                     /db_xref="UniProtKB/Swiss-Prot:P03693"
                     /db_xref="GeneID:1261042"
                     /translation="MEKDKSLITFLEMLDTAMAQRMLADLSDHERRSPQLYNAINKLL
                     DRHKFQIGKLQPDVHILGGLAGALEEYKEKVGDNGLTDDDIYTLQ"
     regulatory      34001
                     /regulatory_class="promoter"
                     /note="E. coli promoter E[6]"
     misc_structure  34021
                     /note="RNase III site R18.5"
     gene            34082..34513
                     /locus_tag="T7delholinF1_55"
                     /note="gene 18.5"
                     /db_xref="GeneID:1261067"
     CDS             34082..34513
                     /locus_tag="T7delholinF1_55"
                     /note="analog of phage lambda protein Rz, a cell lysis
                     protein. Rz and gp18.5 share distant sequence similarity,
                     similar function, and a similar genome neighborhood. In
                     T7, gp18.5 interacts with gp18.7, a lambda RZ1-like lysis
                     protein. Other names: gp18.5"
                     /codon_start=1
                     /transl_table=11
                     /product="phage lambda Rz-like lysis protein"
                     /protein_id="QWY13927.1"
                     /db_xref="GOA:P03803"
                     /db_xref="UniProtKB/Swiss-Prot:P03803"
                     /db_xref="GeneID:1261067"
                     /translation="MLEFLRKLIPWVLAGMLFGLGWHLGSDSMDAKWKQEVHNEYVKR
                     VEAAKSTQRAIDAVSAKYQEDLAALEGSTDRIISDLRSDNKRLRVRVKTTGTSDGQCG
                     FEPDGRAELDDRDAKRILAVTQKGDAWIRALQDTIRELQRK"
     gene            34197..34448
                     /locus_tag="T7delholinF1_56"
                     /note="gene 18.7"
                     /db_xref="GeneID:1261057"
     CDS             34197..34448
                     /locus_tag="T7delholinF1_56"
                     /note="in Enterobacteria phage T7, this protein interacts
                     with gp18.5 and is expressed from the -1 frame of a gene
                     completely overlapping gene 18.5. This suggests that it
                     may be an analog of lambda lysis protein Rz1. Other names:
                     gp18.7."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lambda Rz1-like protein"
                     /protein_id="QWY13928.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03788"
                     /db_xref="GeneID:1261057"
                     /translation="MSTLRELRLRRALKEQSMRYLLSIKKTLPRWKGALIGLFLICVA
                     TISGCASESKLPEPPMVSVDSSLMVEPNLTTEMLNVFSQ"
     gene            34535..36295
                     /locus_tag="T7delholinF1_57"
                     /note="gene 19"
                     /db_xref="GeneID:1261062"
     CDS             34535..36295
                     /locus_tag="T7delholinF1_57"
                     /note="gene 19"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA maturation protein"
                     /protein_id="QWY13929.1"
                     /db_xref="GOA:P03694"
                     /db_xref="UniProtKB/Swiss-Prot:P03694"
                     /db_xref="GeneID:1261062"
                     /translation="MSTQSNRNALVVAQLKGDFVAFLFVLWKALNLPVPTKCQIDMAK
                     VLANGDNKKFILQAFRGIGKSFITCAFVVWSLWRDPQLKILIVSASKERADANSIFIK
                     NIIDLLPFLSELKPRPGQRDSVISFDVGPANPDHSPSVKSVGITGQLTGSRADIIIAD
                     DVEIPSNSATMGAREKLWTLVQEFAALLKPLPSSRVIYLGTPQTEMTLYKELEDNRGY
                     TTIIWPALYPRTREENLYYSQRLAPMLRAEYDENPEALAGTPTDPVRFDRDDLREREL
                     EYGKAGFTLQFMLNPNLSDAEKYPLRLRDAIVAALDLEKAPMHYQWLPNRQNIIEDLP
                     NVGLKGDDLHTYHDCSNNSGQYQQKILVIDPSGRGKDETGYAVLYTLNGYIYLMEAGG
                     FRDGYSDKTLELLAKKAKQWGVQTVVYESNFGDGMFGKVFSPILLKHHNCAMEEIRAR
                     GMKEMRICDTLEPVMQTHRLVIRDEVIRADYQSARDVDGKHDVKYSLFYQMTRITREK
                     GALAHDDRLDALALGIEYLRESMQLDSVKVEGEVLADFLEEHMMRPTVAATHIIEMSV
                     GGVDVYSEDDEGYGTSFIEW"
     gene            35181..35438
                     /locus_tag="T7delholinF1_58"
                     /note="gene 19.2"
                     /db_xref="GeneID:1261064"
     CDS             35181..35438
                     /locus_tag="T7delholinF1_58"
                     /note="gene 19.2"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13930.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03789"
                     /db_xref="GeneID:1261064"
                     /translation="MGTQPLSGLLCTQGHVKRTSITHSVLLLCYALSTMRTLRHLLGL
                     QQTQCALTVMTCASVSWNTVRLALRYSSCLTLTLVMPRSTR"
     gene            35718..35891
                     /locus_tag="T7delholinF1_59"
                     /note="gene 19.3"
                     /db_xref="GeneID:1261066"
     CDS             35718..35891
                     /locus_tag="T7delholinF1_59"
                     /note="gene 19.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13931.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03790"
                     /db_xref="GeneID:1261066"
                     /translation="MATPIRPLSYSLRRQSNGESKRLSTRVTSVTVCSVRYSVLSFLN
                     TTTVRWKRFVPVV"
     regulatory      36394
                     /regulatory_class="promoter"
                     /note="T7 promoter phiOR"
     gene            36554..36703
                     /locus_tag="T7delholinF1_60"
                     /note="gene 19.5"
                     /db_xref="GeneID:1261068"
     CDS             36554..36703
                     /locus_tag="T7delholinF1_60"
                     /note="gene 19.5"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWY13932.1"
                     /db_xref="UniProtKB/Swiss-Prot:P03804"
                     /db_xref="GeneID:1261068"
                     /translation="MFRLLLNLLRHRVTYRFLVVLCADLGYASLTGDLSSLESVVCSI
                     LTCSD"
BASE COUNT         9956 a         8358 c         9550 g         9078 t
ORIGIN      
        1 tctcacagtg tacggaccta aagttccccc atagggggta cctaaagccc agccaatcac
       61 ctaaagtcaa ccttcggttg accttgaggg ttccctaagg gttggggatg acccttgggt
      121 ttgtctttgg gtgttacctt gagtgtctct ctgtgtccct atctgttaca gtctcctaaa
      181 gtatcctcct aaagtcacct cctaacgtcc atcctaaagc caacacctaa agcctacacc
      241 taaagaccca tcaagtcaac gcctatctta aagtttaaac ataaagacca gacctaaaga
      301 ccagacctaa agacactaca taaagaccag acctaaagac gccttgttgt tagccataaa
      361 gtgataacct ttaatcattg tctttattaa tacaactcac tataaggaga gacaacttaa
      421 agagacttaa aagattaatt taaaatttat caaaaagagt attgacttaa agtctaacct
      481 ataggatact tacagccatc gagagggaca cggcgaatag ccatcccaat cgacaccggg
      541 gtcaaccgga taagtagaca gcctgataag tcgcacgaca gaaagaaatt gaccgcgcta
      601 aggcccgtaa agaacgtcac gaggggcgct tagaggcacg cagattcaaa cgtcgcaacc
      661 gcaaggcacg taaagcacac aaagctaagc gcgaaagaat gcttgctgcg tggcgatggg
      721 ctgaacgtca agaacggcgt aaccatgagg tagctgtaga tgtactagga agaaccaata
      781 acgctatgct ctgggtcaac atgttctctg gggactttaa ggcgcttgag gaacgaatcg
      841 cgctgcactg gcgtaatgct gaccggatgg ctatcgctaa tggtcttacg ctcaacattg
      901 ataagcaact tgacgcaatg ttaatgggct gatagtctta tcttacaggt catctgcggg
      961 tggcctgaat aggtacgatt tactaactgg aagaggcact aaatgaacac gattaacatc
     1021 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
     1081 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
     1141 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
     1201 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
     1261 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
     1321 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
     1381 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
     1441 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
     1501 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
     1561 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
     1621 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
     1681 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
     1741 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
     1801 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
     1861 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
     1921 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
     1981 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat
     2041 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac
     2101 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc
     2161 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat
     2221 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt
     2281 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg
     2341 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac
     2401 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac
     2461 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat
     2521 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg
     2581 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc
     2641 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc
     2701 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca
     2761 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct
     2821 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact
     2881 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc
     2941 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg
     3001 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc
     3061 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg
     3121 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat
     3181 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc
     3241 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat
     3301 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa
     3361 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct
     3421 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa
     3481 gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac
     3541 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct
     3601 aaaggtaact tgaacttccg tgacatctta gagtcggact tcgcgttcgc gtaacgccaa
     3661 atcaatacga ctcactatag agggacaaac tcaaggtcat tcgcaagagt ggcctttatg
     3721 attgaccttc ttccggttaa tacgactcac tataggagaa ccttaaggtt taactttaag
     3781 acccttaagt gttaattaga gatttaaatt aaagaattac taagagagga ctttaagtat
     3841 gcgtaacttc gaaaagatga ccaaacgttc taaccgtaat gctcgtgact tcgaggcaac
     3901 caaaggtcgc aagttgaata agactaagcg tgaccgctct cacaagcgta gctgggaggg
     3961 tcagtaagat gggacgttta tatagtggta atctggcagc attcaaggca gcaacaaaca
     4021 agctgttcca gttagactta gcggtcattt atgatgactg gtatgatgcc tatacaagaa
     4081 aagattgcat acggttacgt attgaggaca ggagtggaaa cctgattgat actagcacct
     4141 tctaccacca cgacgaggac gttctgttca atatgtgtac tgattggttg aaccatatgt
     4201 atgaccagtt gaaggactgg aagtaatacg actcagtata gggacaatgc ttaaggtcgc
     4261 tctctaggag tggccttagt catttaacca ataggagata aacattatga tgaacattaa
     4321 gactaacccg tttaaagccg tgtctttcgt agagtctgcc attaagaagg ctctggataa
     4381 cgctgggtat cttatcgctg aaatcaagta cgatggtgta cgcgggaaca tctgcgtaga
     4441 caatactgct aacagttact ggctctctcg tgtatctaaa acgattccgg cactggagca
     4501 cttaaacggg tttgatgttc gctggaagcg tctactgaac gatgaccgtt gcttctacaa
     4561 agatggcttt atgcttgatg gggaactcat ggtcaagggc gtagacttta acacagggtc
     4621 cggcctactg cgtaccaaat ggactgacac gaagaaccaa gagttccatg aagagttatt
     4681 cgttgaacca atccgtaaga aagataaagt tccctttaag ctgcacactg gacaccttca
     4741 cataaaactg tacgctatcc ttccgctgca catcgtggag tctggagaag actgtgatgt
     4801 catgacgttg ctcatgcagg aacacgttaa gaacatgctg cctctgctac aggaatactt
     4861 ccctgaaatc gaatggcaag cggctgaatc ttacgaggtc tacgatatgg tagaactaca
     4921 gcaactgtac gagcagaagc gagcagaagg ccatgagggt ctcattgtga aagacccgat
     4981 gtgtatctat aagcgcggta agaaatctgg ctggtggaaa atgaaacctg agaacgaagc
     5041 tgacggtatc attcagggtc tggtatgggg tacaaaaggt ctggctaatg aaggtaaagt
     5101 gattggtttt gaggtgcttc ttgagagtgg tcgtttagtt aacgccacga atatctctcg
     5161 cgccttaatg gatgagttca ctgagacagt aaaagaggcc accctaagtc aatggggatt
     5221 ctttagccca tacggtattg gcgacaacga tgcttgtact attaaccctt acgatggctg
     5281 ggcgtgtcaa attagctaca tggaggaaac acctgatggc tctttgcggc acccatcgtt
     5341 cgtaatgttc cgtggcaccg aggacaaccc tcaagagaaa atgtaatcac actggctcac
     5401 cttcgggtgg gcctttctgc gtttataagg agacacttta tgtttaagaa ggttggtaaa
     5461 ttccttgcgg ctttggcagc tatcctgacg cttgcgtata ttcttgcggt ataccctcaa
     5521 gtagcactag tagttggcgc ttgttactta gcggcagtgt gtgcttgcgt gtggagtata
     5581 gttaactggt aatacgactc actaaaggag gtacacacca tgatgtactt aatgccatta
     5641 ctcatcgtca ttgtaggatg ccttgcgctc cactgtagcg atgatgatat gccagatggt
     5701 cacgcttaat acgactcact aaaggagaca ctatatgttt cgacttcatt acaacaaaag
     5761 cgttaagaat ttcacggttc gccgtgctga ccgttcaatc gtatgtgcga gcgagcgccg
     5821 agctaagata cctcttattg gtaacacagt tcctttggca ccgagcgtcc acatcattat
     5881 cacccgtggt gactttgaga aagcaataga caagaaacgt ccggttctta gtgtggcagt
     5941 gactcgcttc ccgttcgtcc gtctgttact caaacgaatc aaggaggtgt tctgatggga
     6001 ctgttagatg gtgaagcctg ggaaaaagaa aacccgccag tacaagcaac tgggtgtata
     6061 gcttgcttag agaaagatga ccgttatcca cacacctgta acaaaggagc taacgatatg
     6121 accgaacgtg aacaagagat gatcattaag ttgatagaca ataatgaagg tcgcccagat
     6181 gatttgaatg gctgcggtat tctctgctcc aatgtccctt gccacctctg ccccgcaaat
     6241 aacgatcaaa aaataacctt aggtgaaatc cgagcgatgg acccacgtaa accacatctg
     6301 aataaacctg aggtaactcc tacagatgac cagccttccg ctgagacaat cgaaggtgtc
     6361 actaagcctt cccactacat gctgtttgac gacattgagg ctatcgaagt gattgctcgt
     6421 tcaatgaccg ttgagcagtt caagggatac tgcttcggta acatcttaaa gtacagacta
     6481 cgtgctggta agaagtcaga gttagcgtac ttagagaaag acctagcgaa agcagacttc
     6541 tataaagaac tctttgagaa acataaggat aaatgttatg cataacttca agtcaacccc
     6601 acctgccgac agcctatctg atgacttcac atcttgctca gagtggtgcc gaaagatgtg
     6661 ggaagagaca ttcgacgatg cgtacatcaa gctgtatgaa ctttggaaat cgagaggtca
     6721 atgactatgt caaacgtaaa tacaggttca cttagtgtgg acaacaagaa gttttgggct
     6781 accgtagagt cctcggagca ttccttcgag gttccaatct acgctgagac cctagacgaa
     6841 gctctggagt tagccgaatg gcaatacgtt ccggctggct ttgaggttac tcgtgtgcgt
     6901 ccttgtgtag caccgaagta atacgactca ctattaggga agactccctc tgagaaacca
     6961 aacgaaacct aaaggagatt aacattatgg ctaagaagat tttcacctct gcgctgggta
     7021 ccgctgaacc ttacgcttac atcgccaagc cggactacgg caacgaagag cgtggctttg
     7081 ggaaccctcg tggtgtctat aaagttgacc tgactattcc caacaaagac ccgcgctgcc
     7141 agcgtatggt cgatgaaatc gtgaagtgtc acgaagaggc ttatgctgct gccgttgagg
     7201 aatacgaagc taatccacct gctgtagctc gtggtaagaa accgctgaaa ccgtatgagg
     7261 gtgacatgcc gttcttcgat aacggtgacg gtacgactac ctttaagttc aaatgctacg
     7321 cgtctttcca agacaagaag accaaagaga ccaagcacat caatctggtt gtggttgact
     7381 caaaaggtaa gaagatggaa gacgttccga ttatcggtgg tggctctaag ctgaaagtta
     7441 aatattctct ggttccatac aagtggaaca ctgctgtagg tgcgagcgtt aagctgcaac
     7501 tggaatccgt gatgctggtc gaactggcta cctttggtgg cggtgaagac gattgggctg
     7561 acgaagttga agagaacggc tatgttgcct ctggttctgc caaagcgagc aaaccacgcg
     7621 acgaagaaag ctgggacgaa gacgacgaag agtccgagga agcagacgaa gacggagact
     7681 tctaagtgga actgcgggag aaaatccttg agcgaatcaa ggtgacttcc tctgggtgtt
     7741 gggagtggca gggcgctacg aacaataaag ggtacgggca ggtgtggtgc agcaataccg
     7801 gaaaggttgt ctactgtcat cgcgtaatgt ctaatgctcc gaaaggttct accgtcctgc
     7861 actcctgtga taatccatta tgttgtaacc ctgaacacct atccatagga actccaaaag
     7921 agaactccac tgacatggta aataagggtc gctcacacaa ggggtataaa ctttcagacg
     7981 aagacgtaat ggcaatcatg gaatccagcg agtccaatgt atccttagct cgcacctatg
     8041 gtgtctccca acagactatt tgtgatatac gcaaagggag gcgacatggc aggttacggc
     8101 gctaaaggaa tccgaaaggt tggagcgttt cgctctggcc tagaggacaa ggtttcaaag
     8161 cagttggaat caaaaggtat taaattcgag tatgaagagt ggaaagtgcc ttatgtaatt
     8221 ccggcgagca atcacactta cactccagac ttcttacttc caaacggtat attcgttgag
     8281 acaaagggtc tgtgggaaag cgatgataga aagaagcact tattaattag ggagcagcac
     8341 cccgagctag acatccgtat tgtcttctca agctcacgta ctaagttata caaaggttct
     8401 ccaacgtctt atggagagtt ctgcgaaaag catggtatta agttcgctga taaactgata
     8461 cctgctgagt ggataaagga acccaagaag gaggtcccct ttgatagatt aaaaaggaaa
     8521 ggaggaaaga aataatggct cgtgtacagt ttaaacaacg tgaatctact gacgcaatct
     8581 ttgttcactg ctcggctacc aagccaagtc agaatgttgg tgtccgtgag attcgccagt
     8641 ggcacaaaga gcagggttgg ctcgatgtgg gataccactt tatcatcaag cgagacggta
     8701 ctgtggaggc aggacgagat gagatggctg taggctctca cgctaagggt tacaaccaca
     8761 actctatcgg cgtctgcctt gttggtggta tcgacgataa aggtaagttc gacgctaact
     8821 ttacgccagc ccaaatgcaa tcccttcgct cactgcttgt cacactgctg gctaagtacg
     8881 aaggcgctgt gcttcgcgcc catcatgagg tggcgccgaa ggcttgccct tcgttcgacc
     8941 ttaagcgttg gtgggagaag aacgaactgg tcacttctga ccgtggataa tgatctattg
     9001 gaagtcgttg cgtggattta tagaactagg agggaattgc atggacaatt cgcacgattc
     9061 cgatagtgta tttctttacc acattccttg tgacaactgt gggagtagtg atgggaactc
     9121 gctgttctct gacggacaca cgttctgcta cgtatgcgag aagtggactg ctggtaatga
     9181 agacactaaa gagagggctt caaaacggaa accctcagga ggtaaaccaa tgacttacaa
     9241 cgtgtggaac ttcggggaat ccaatggacg ctactccgcg ttaactgcga gaggaatctc
     9301 caaggaaacc tgtcagaagg ctggctactg gattgccaaa gtagacggtg tgatgtacca
     9361 agtggctgac tatcgggacc agaacggcaa cattgtgagt cagaaggttc gagataaaga
     9421 taagaacttt aagaccactg gtagtcacaa gagtgacgct ctgttcggga agcacttgtg
     9481 gaatggtggt aagaagattg tcgttacaga aggtgaaatc gacatgctta ccgtgatgga
     9541 acttcaagac tgtaagtatc ctgtagtgtc gttgggtcac ggtgcctctg ccgctaagaa
     9601 gacatgcgct gccaactacg aatactttga ccagttcgaa cagattatct taatgttcga
     9661 tatggacgaa gcagggcgca aagcagtcga agaggctgca caggttctac ctgctggtaa
     9721 ggtacgagtg gcagttcttc cgtgtaagga tgcaaacgag tgtcacctaa atggtcacga
     9781 ccgtgaaatc atggagcaag tgtggaatgc tggtccttgg attcctgatg gtgtggtatc
     9841 ggctctttcg ttacgtgaac gaatccgtga gcacctatcg tccgaggaat cagtaggttt
     9901 acttttcagt ggctgcactg gtatcaacga taagacctta ggtgcccgtg gtggtgaagt
     9961 cattatggtc acttccggtt ccggtatggg taagtcaacg ttcgtccgtc aacaagctct
    10021 acaatggggc acagcgatgg gcaagaaggt aggcttagcg atgcttgagg agtccgttga
    10081 ggagaccgct gaggacctta taggtctaca caaccgtgtc cgactgagac aatccgactc
    10141 actaaagaga gagattattg agaacggtaa gttcgaccaa tggttcgatg aactgttcgg
    10201 caacgatacg ttccatctat atgactcatt cgccgaggct gagacggata gactgctcgc
    10261 taagctggcc tacatgcgct caggcttggg ctgtgacgta atcattctag accacatctc
    10321 aatcgtcgta tccgcttctg gtgaatccga tgagcgtaag atgattgaca acctgatgac
    10381 caagctcaaa gggttcgcta agtcaactgg ggtggtgctg gtcgtaattt gtcaccttaa
    10441 gaacccagac aaaggtaaag cacatgagga aggtcgcccc gtttctatta ctgacctacg
    10501 tggttctggc gcactacgcc aactatctga tactattatt gcccttgagc gtaatcagca
    10561 aggcgatatg cctaaccttg tcctcgttcg tattctcaag tgccgcttta ctggtgatac
    10621 tggtatcgct ggctacatgg aatacaacaa ggaaaccgga tggcttgaac catcaagtta
    10681 ctcaggggaa gaagagtcac actcagagtc aacagactgg tccaacgaca ctgacttctg
    10741 acaggattct tgatgacttt ccagacgact acgagaagtt tcgctggaga gtcccattct
    10801 aatacgactc actaaaggag acacaccatg ttcaaactga ttaagaagtt aggccaactg
    10861 ctggttcgta tgtacaacgt ggaagccaag cgactgaacg atgaggctcg taaagaggcc
    10921 acacagtcac gcgctctggc gattcgctcc aacgaactgg ctgacagtgc atccactaaa
    10981 gttaccgagg ctgcccgtgt ggcaaaccaa gctcaacagc tttccaaatt ctttgagtaa
    11041 tcaaacagga gaaaccatta tgtctaacgt agctgaaact atccgtctat ccgatacagc
    11101 tgaccagtgg aaccgtcgag tccacatcaa cgttcgcaac ggtaaggcga ctatggttta
    11161 ccgctggaag gactctaagt cctctaagaa tcacactcag cgtatgacgt tgacagatga
    11221 gcaagcactg cgtctggtca atgcgcttac caaagctgcc gtgacagcaa ttcatgaagc
    11281 tggtcgcgtc aatgaagcta tggctatcct cgacaagatt gataactaag agtggtatcc
    11341 tcaaggtcgc caaagtggtg gccttcatga atactattcg actcactata ggagatatta
    11401 ccatgcgtga ccctaaagtt atccaagcag aaatcgctaa actggaagct gaactggagg
    11461 acgttaagta ccatgaagct aagactcgct ccgctgttca catcttgaag aacttaggct
    11521 ggacttggac aagacagact ggctggaaga aaccagaagt taccaagctg agtcataagg
    11581 tgttcgataa ggacactatg acccacatca aggctggtga ttgggttaag gttgacatgg
    11641 gagttgttgg tggatacggc tacgtccgct cagttagtgg caaatatgca caagtgtcat
    11701 acatcacagg tgttactcca cgcggtgcaa tcgttgccga taagaccaac atgattcaca
    11761 caggtttctt gacagttgtt tcatatgaag agattgttaa gtcacgataa tcaataggag
    11821 aaatcaatat gatcgtttct gacatcgaag ctaacgccct cttagagagc gtcactaagt
    11881 tccactgcgg ggttatctac gactactcca ccgctgagta cgtaagctac cgtccgagtg
    11941 acttcggtgc gtatctggat gcgctggaag ccgaggttgc acgaggcggt cttattgtgt
    12001 tccacaacgg tcacaagtat gacgttcctg cattgaccaa actggcaaag ttgcaattga
    12061 accgagagtt ccaccttcct cgtgagaact gtattgacac ccttgtgttg tcacgtttga
    12121 ttcattccaa cctcaaggac accgatatgg gtcttctgcg ttccggcaag ttgcccggaa
    12181 aacgctttgg gtctcacgct ttggaggcgt ggggttatcg cttaggcgag atgaagggtg
    12241 aatacaaaga cgactttaag cgtatgcttg aagagcaggg tgaagaatac gttgacggaa
    12301 tggagtggtg gaacttcaac gaagagatga tggactataa cgttcaggac gttgtggtaa
    12361 ctaaagctct ccttgagaag ctactctctg acaaacatta cttccctcct gagattgact
    12421 ttacggacgt aggatacact acgttctggt cagaatccct tgaggccgtt gacattgaac
    12481 atcgtgctgc atggctgctc gctaaacaag agcgcaacgg gttcccgttt gacacaaaag
    12541 caatcgaaga gttgtacgta gagttagctg ctcgccgctc tgagttgctc cgtaaattga
    12601 ccgaaacgtt cggctcgtgg tatcagccta aaggtggcac tgagatgttc tgccatccgc
    12661 gaacaggtaa gccactacct aaataccctc gcattaagac acctaaagtt ggtggtatct
    12721 ttaagaagcc taagaacaag gcacagcgag aaggccgtga gccttgcgaa cttgataccc
    12781 gcgagtacgt tgctggtgct ccttacaccc cagttgaaca tgttgtgttt aacccttcgt
    12841 ctcgtgacca cattcagaag aaactccaag aggctgggtg ggtcccgacc aagtacaccg
    12901 ataagggtgc tcctgtggtg gacgatgagg tactcgaagg agtacgtgta gatgaccctg
    12961 agaagcaagc cgctatcgac ctcattaaag agtacttgat gattcagaag cgaatcggac
    13021 agtctgctga gggagacaaa gcatggcttc gttatgttgc tgaggatggt aagattcatg
    13081 gttctgttaa ccctaatgga gcagttacgg gtcgtgcgac ccatgcgttc ccaaaccttg
    13141 cgcaaattcc gggtgtacgt tctccttatg gagagcagtg tcgcgctgct tttggcgctg
    13201 agcaccattt ggatgggata actggtaagc cttgggttca ggctggcatc gacgcatccg
    13261 gtcttgagct acgctgcttg gctcacttca tggctcgctt tgataacggc gagtacgctc
    13321 acgagattct taacggcgac atccacacta agaaccagat agctgctgaa ctacctaccc
    13381 gagataacgc taagacgttc atctatgggt tcctctatgg tgctggtgat gagaagattg
    13441 gacagattgt tggtgctggt aaagagcgcg gtaaggaact caagaagaaa ttccttgaga
    13501 acacccccgc gattgcagca ctccgcgagt ctatccaaca gacacttgtc gagtcctctc
    13561 aatgggtagc tggtgagcaa caagtcaagt ggaaacgccg ctggattaaa ggtctggatg
    13621 gtcgtaaggt acacgttcgt agtcctcacg ctgccttgaa taccctactg caatctgctg
    13681 gtgctctcat ctgcaaactg tggattatca agaccgaaga gatgctcgta gagaaaggct
    13741 tgaagcatgg ctgggatggg gactttgcgt acatggcatg ggtacatgat gaaatccaag
    13801 taggctgccg taccgaagag attgctcagg tggtcattga gaccgcacaa gaagcgatgc
    13861 gctgggttgg agatcactgg aacttccggt gtcttctgga taccgaaggt aagatgggtc
    13921 ctaattgggc gatttgccac tgatacagga ggctactcat gaacgaaaga cacttaacag
    13981 gtgctgcttc tgaaatgcta gtagcctaca aatttaccaa agctgggtac actgtctatt
    14041 accctatgct gactcagagt aaagaggact tggttgtatg taaggatggt aaatttagta
    14101 aggttcaggt taaaacagcc acaacggttc aaaccaacac aggagatgcc aagcaggtta
    14161 ggctaggtgg atgcggtagg tccgaatata aggatggaga ctttgacatt cttgcggttg
    14221 tggttgacga agatgtgctt attttcacat gggacgaagt aaaaggtaag acatccatgt
    14281 gtgtcggcaa gagaaacaaa ggcataaaac tataggagaa attattatgg ctatgacaaa
    14341 gaaatttaaa gtgtccttcg acgttaccgc aaagatgtcg tctgacgttc aggcagtctt
    14401 agagaaagat atgctgcatc tatgtaagca ggtcggctca ggtgcgattg tccccaatgg
    14461 taaacagaag gaaatgattg tccagttcct gacacacggt atggaaggat tgatgacatt
    14521 cgtagtacgt acatcatttc gtgaggccat taaggacatg cacgaagagt atgcagataa
    14581 ggactctttc aaacaatctc ctgcaacagt acgggaggtg ttctgatgtc tgactacctg
    14641 aaagtgctgc aagcaatcaa aagttgccct aagactttcc agtccaacta tgtacggaac
    14701 aatgcgagcc tcgtagcgga ggccgcttcc cgtggtcaca tctcgtgcct gactactagt
    14761 ggacgtaacg gtggcgcttg ggaaatcact gcttccggta ctcgctttct gaaacgaatg
    14821 ggaggatgtg tctaatgtct cgtgaccttg tgactattcc acgcgatgtg tggaacgata
    14881 tacagggcta catcgactct ctggaacgtg agaacgatag ccttaagaat caactaatgg
    14941 aagctgacga atacgtagcg gaactagagg agaaacttaa tggcacttct tgaccttaaa
    15001 caattctatg agttacgtga aggctgcgac gacaagggta tccttgtgat ggacggcgac
    15061 tggctggtct tccaagctat gagtgctgct gagtttgatg cctcttggga ggaagagatt
    15121 tggcaccgat gctgtgacca cgctaaggcc cgtcagattc ttgaggattc cattaagtcc
    15181 tacgagaccc gtaagaaggc ttgggcaggt gctccaattg tccttgcgtt caccgatagt
    15241 gttaactggc gtaaagaact ggttgacccg aactataagg ctaaccgtaa ggccgtgaag
    15301 aaacctgtag ggtactttga gttccttgat gctctctttg agcgcgaaga gttctattgc
    15361 atccgtgagc ctatgcttga gggtgatgac gttatgggag ttattgcttc caatccgtct
    15421 gccttcggtg ctcgtaaggc tgtaatcatc tcttgcgata aggactttaa gaccatccct
    15481 aactgtgact tcctgtggtg taccactggt aacatcctga ctcagaccga agagtccgct
    15541 gactggtggc acctcttcca gaccatcaag ggtgacatca ctgatggtta ctcagggatt
    15601 gctggatggg gtgataccgc cgaggacttc ttgaataacc cgttcataac cgagcctaaa
    15661 acgtctgtgc ttaagtccgg taagaacaaa ggccaagagg ttactaaatg ggttaaacgc
    15721 gaccctgagc ctcatgagac gctttgggac tgcattaagt ccattggcgc gaaggctggt
    15781 atgaccgaag aggatattat caagcagggc caaatggctc gaatcctacg gttcaacgag
    15841 tacaacttta ttgacaagga gatttacctg tggagaccgt agcgtatatt ggtctggatc
    15901 tttgtgttct cggagtgtgc ctcatttcgt ggggcctttg ggacttagcc agaataatca
    15961 agtcgttaca cgacactaag tgataaactc aaggtcccta aattaatacg actcactata
    16021 gggagatagg ggcctttacg attattactt taagatttaa ctctaagagg aatctttatt
    16081 atgttaacac ctattaacca attacttaag aaccctaacg atattccaga tgtacctcgt
    16141 gcaaccgctg agtatctaca ggttcgattc aactatgcgt acctcgaagc gtctggtcat
    16201 ataggactta tgcgtgctaa tggttgtagt gaggcccaca tcttgggttt cattcagggc
    16261 ctacagtatg cctctaacgt cattgacgag attgagttac gcaaggaaca actaagagat
    16321 gatggggagg attgacacta tgtgtttctc accgaaaatt aaaactccga agatggatac
    16381 caatcagatt cgagccgttg agccagcgcc tctgacccaa gaagtgtcaa gcgtggagtt
    16441 cggtgggtct tctgatgaga cggataccga gggcaccgaa gtgtctggac gcaaaggcct
    16501 caaggtcgaa cgtgatgatt ccgtagcgaa gtctaaagcc agcggcaatg gctccgctcg
    16561 tatgaaatct tccatccgta agtccgcatt tggaggtaag aagtgatgtc tgagttcaca
    16621 tgtatggagg ctaagagtcg cttccgtgca atccggtgga ctgtggaaca ccttgggttg
    16681 cctaaaggat tcgaaggaca ctttgtgggc tacagcctct acgtagacga agtgatggac
    16741 atgtctggtt gccgtgaaga gtacattctg gactctaccg gaaaacatgt agcgtacttc
    16801 gcgtggtgcg taagctgtga cattcaccac aaaggagaca ttctggatgt aacgtccgtt
    16861 gtcattaatc ctgaggcaga ctctaagggc ttacagcgat tcctagcgaa acgctttaag
    16921 taccttgcgg aactccacga ttgcgattgg gtgtctcgtt gtaagcatga aggcgagaca
    16981 atgcgtgtat actttaagga ggtataagtt atgggtaaga aagttaagaa ggccgtgaag
    17041 aaagtcacca agtccgttaa gaaagtcgtt aaggaagggg ctcgtccggt taaacaggtt
    17101 gctggcggtc tagctggtct ggctggtggt actggtgaag cacagatggt ggaagtacca
    17161 caagctgccg cacagattgt tgacgtacct gagaaagagg tttccactga ggacgaagca
    17221 cagacagaaa gcggacgcaa gaaagctcgt gctggcggta agaaatcctt gagtgtagcc
    17281 cgtagctccg gtggcggtat caacatttaa tcaggaggtt atcgtggaag actgcattga
    17341 atggaccgga gatgtcaact ctaagggtta tggtcgtaag tgggttaatg gtaaacttgt
    17401 gactccacat aggcacatct atgaggagac atatggtcca gttccaacag gaattgtggt
    17461 gatgcatatc tgcgataacc ctaggtgcta taacataaag caccttacgc ttggaactcc
    17521 aaaggataat tccgaggaca tggttaccaa aggtagacag gctaaaggag aggaactaag
    17581 caagaaactt acagagtcag acgttctcgc tatacgctct tcaaccttaa gccaccgctc
    17641 cttaggagaa ctgtatggag tcagtcaatc aaccataacg cgaatactac agcgtaagac
    17701 atggagacac atttaatggc tgagaaacga acaggacttg cggaggatgg cgcaaagtct
    17761 gtctatgagc gtttaaagaa cgaccgtgct ccctatgaga cacgcgctca gaattgcgct
    17821 caatatacca tcccatcatt gttccctaag gactccgata acgcctctac agattatcaa
    17881 actccgtggc aagccgtggg cgctcgtggt ctgaacaatc tagcctctaa gctcatgctg
    17941 gctctattcc ctatgcagac ttggatgcga cttactatat ctgaatatga agcaaagcag
    18001 ttactgagcg accccgatgg actcgctaag gtcgatgagg gcctctcgat ggtagagcgt
    18061 atcatcatga actacattga gtctaacagt taccgcgtga ctctctttga ggctctcaaa
    18121 cagttagtcg tagctggtaa cgtcctgctg tacctaccgg aaccggaagg gtcaaactat
    18181 aatcccatga agctgtaccg attgtcttct tatgtggtcc aacgagacgc attcggcaac
    18241 gttctgcaaa tggtgactcg tgaccagata gcttttggtg ctctccctga ggacatccgt
    18301 aaggctgtag aaggtcaagg tggtgagaag aaagctgatg agacaatcga cgtgtacact
    18361 cacatctatc tggatgagga ctcaggtgaa tacctccgat acgaagaggt cgagggtatg
    18421 gaagtccaag gctccgatgg gacttatcct aaagaggctt gcccatacat cccgattcgg
    18481 atggtcagac tagatggtga atcctacggt cgttcgtaca ttgaggaata cttaggtgac
    18541 ttacggtccc ttgaaaatct ccaagaggct atcgtcaaga tgtccatgat tagctctaag
    18601 gttatcggct tagtgaatcc tgctggtatc acccagccac gccgactgac caaagctcag
    18661 actggtgact tcgttactgg tcgtccagaa gacatctcgt tcctccaact ggagaagcaa
    18721 gcagacttta ctgtagctaa agccgtaagt gacgctatcg aggctcgcct ttcgtttgcc
    18781 tttatgttga actctgcggt tcagcgtaca ggtgaacgtg tgaccgccga agagattcgg
    18841 tatgtagctt ctgaacttga agatacttta ggtggtgtct actctatcct ttctcaagaa
    18901 ttacaattgc ctctggtacg agtgctcttg aagcaactac aagccacgca acagattcct
    18961 gagttaccta aggaagccgt agagccaacc attagtacag gtctggaagc aattggtcga
    19021 ggacaagacc ttgataagct ggagcggtgt gtcactgcgt gggctgcact ggcacctatg
    19081 cgggacgacc ctgatattaa ccttgcgatg attaagttac gtattgccaa cgctatcggt
    19141 attgacactt ctggtattct actcaccgaa gaacagaagc aacagaagat ggcccaacag
    19201 tctatgcaaa tgggtatgga taatggtgct gctgcgctgg ctcaaggtat ggctgcacaa
    19261 gctacagctt cacctgaggc tatggctgct gccgctgatt ccgtaggttt acagccggga
    19321 atttaatacg actcactata gggagacctc atctttgaaa tgagcgatga caagaggttg
    19381 gagtcctcgg tcttcctgta gttcaacttt aaggagacaa taataatggc tgaatctaat
    19441 gcagacgtat atgcatcttt tggcgtgaac tccgctgtga tgtctggtgg ttccgttgag
    19501 gaacatgagc agaacatgct ggctcttgat gttgctgccc gtgatggcga tgatgcaatc
    19561 gagttagcgt cagacgaagt ggaaacagaa cgtgacctgt atgacaactc tgacccgttc
    19621 ggtcaagagg atgacgaagg ccgcattcag gttcgtatcg gtgatggctc tgagccgacc
    19681 gatgtggaca ctggagaaga aggcgttgag ggcaccgaag gttccgaaga gtttacccca
    19741 ctgggcgaga ctccagaaga actggtagct gcctctgagc aacttggtga gcacgaagag
    19801 ggcttccaag agatgattaa cattgctgct gagcgtggca tgagtgtcga gaccattgag
    19861 gctatccagc gtgagtacga ggagaacgaa gagttgtccg ccgagtccta cgctaagctg
    19921 gctgaaattg gctacacgaa ggctttcatt gactcgtata tccgtggtca agaagctctg
    19981 gtggagcagt acgtaaacag tgtcattgag tacgctggtg gtcgtgaacg ttttgatgca
    20041 ctgtataacc accttgagac gcacaaccct gaggctgcac agtcgctgga taatgcgttg
    20101 accaatcgtg acttagcgac cgttaaggct atcatcaact tggctggtga gtctcgcgct
    20161 aaggcgttcg gtcgtaagcc aactcgtagt gtgactaatc gtgctattcc ggctaaacct
    20221 caggctacca agcgtgaagg ctttgcggac cgtagcgaga tgattaaagc tatgagtgac
    20281 cctcggtatc gcacagatgc caactatcgt cgtcaagtcg aacagaaagt aatcgattcg
    20341 aacttctgat agacttcgaa attaatacga ctcactatag ggagaccaca acggtttccc
    20401 cctagaaata attttgttta actttaagaa ggagatatac atatggctag catgactggt
    20461 ggacagcaaa tgggtactaa ccaaggtaaa ggtgtagttg ctgctggaga taaactggcg
    20521 ttgttcttga aggtatttgg cggtgaagtc ctgactgcgt tcgctcgtac ctccgtgacc
    20581 acttctcgcc acatggtacg ttccatctcc agcggtaaat ccgctcagtt ccctgttctg
    20641 ggtcgcactc aggcagcgta tctggctccg ggcgagaacc tcgacgataa acgtaaggac
    20701 atcaaacaca ccgagaaggt aatcaccatt gacggtctcc tgacggctga cgttctgatt
    20761 tatgatattg aggacgcgat gaaccactac gacgttcgct ctgagtatac ctctcagttg
    20821 ggtgaatctc tggcgatggc tgcggatggt gcggttctgg ctgagattgc cggtctgtgt
    20881 aacgtggaaa gcaaatataa tgagaacatc gagggcttag gtactgctac cgtaattgag
    20941 accactcaga acaaggccgc acttaccgac caagttgcgc tgggtaagga gattattgcg
    21001 gctctgacta aggctcgtgc ggctctgacc aagaactatg ttccggctgc tgaccgtgtg
    21061 ttctactgtg acccagatag ctactctgcg attctggcag cactgatgcc gaacgcagca
    21121 aactacgctg ctctgattga ccctgagaag ggttctatcc gcaacgttat gggctttgag
    21181 gttgtagaag ttccgcacct caccgctggt ggtgctggta ccgctcgtga gggcactact
    21241 ggtcagaagc acgtcttccc tgccaataaa ggtgagggta atgtcaaggt tgctaaggac
    21301 aacgttatcg gcctgttcat gcaccgctct gcggtaggta ctgttaagct gcgtgacttg
    21361 gctctggagc gcgctcgccg tgctaacttc caagcggacc agattatcgc taagtacgca
    21421 atgggccacg gtggtcttcg cccagaagct gcaggagctg tcgtattcca gtcaggtgtg
    21481 atgctcgggg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagtaacta
    21541 gttaacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
    21601 tatgcgctca tacgatatga acgttgagac tgccgctgag ttatcagctg tgaacgacat
    21661 tctggcgtct atcggtgaac ctccggtatc aacgctggaa ggtgacgcta acgcagatgc
    21721 agcgaacgct cggcgtattc tcaacaagat taaccgacag attcaatctc gtggatggac
    21781 gttcaacatt gaggaaggca taacgctact acctgatgtt tactccaacc tgattgtata
    21841 cagtgacgac tatttatccc taatgtctac ttccggtcaa tccatctacg ttaaccgagg
    21901 tggctatgtg tatgaccgaa cgagtcaatc agaccgcttt gactctggta ttactgtgaa
    21961 cattattcgt ctccgcgact acgatgagat gcctgagtgc ttccgttact ggattgtcac
    22021 caaggcttcc cgtcagttca acaaccgatt ctttggggca ccggaagtag agggtgtact
    22081 ccaagaagag gaagatgagg ctagacgtct ctgcatggag tatgagatgg actacggtgg
    22141 gtacaatatg ctggatggag atgcgttcac ttctggtcta ctgactcgct aacattaata
    22201 aataaggagg ctctaatggc actcattagc caatcaatca agaacttgaa gggtggtatc
    22261 agccaacagc ctgacatcct tcgttatcca gaccaagggt cacgccaagt taacggttgg
    22321 tcttcggaga ccgagggcct ccaaaagcgt ccacctcttg ttttcttaaa tacacttgga
    22381 gacaacggtg cgttaggtca agctccgtac atccacctga ttaaccgaga tgagcacgaa
    22441 cagtattacg ctgtgttcac tggtagcgga atccgagtgt tcgacctttc tggtaacgag
    22501 aagcaagtta ggtatcctaa cggttccaac tacatcaaga ccgctaatcc acgtaacgac
    22561 ctgcgaatgg ttactgtagc agactatacg ttcatcgtta accgtaacgt tgttgcacag
    22621 aagaacacaa agtctgtcaa cttaccgaat tacaacccta atcaagacgg attgattaac
    22681 gttcgtggtg gtcagtatgg tagggaacta attgtacaca ttaacggtaa agacgttgcg
    22741 aagtataaga taccagatgg tagtcaacct gaacacgtaa acaatacgga tgcccaatgg
    22801 ttagctgaag agttagccaa gcagatgcgc actaacttgt ctgattggac tgtaaatgta
    22861 gggcaagggt tcatccatgt gaccgcacct agtggtcaac agattgactc cttcacgact
    22921 aaagatggct acgcagacca gttgattaac cctgtgaccc actacgctca gtcgttctct
    22981 aagctgccac ctaatgctcc taacggctac atggtgaaaa tcgtagggga cgcctctaag
    23041 tctgccgacc agtattacgt tcggtatgac gctgagcgga aagtttggac tgagacttta
    23101 ggttggaaca ctgaggacca agttctatgg gaaaccatgc cacacgctct tgtgcgagcc
    23161 gctgacggta atttcgactt caagtggctt gagtggtctc ctaagtcttg tggtgacgtt
    23221 gacaccaacc cttggccttc ttttgttggt tcaagtatta acgatgtgtt cttcttccgt
    23281 aaccgcttag gattccttag tggggagaac atcatattga gtcgtacagc caaatacttc
    23341 aacttctacc ctgcgtccat tgcgaacctt agtgatgacg accctataga cgtagctgtg
    23401 agtaccaacc gaatagcaat ccttaagtac gccgttccgt tctcagaaga gttactcatc
    23461 tggtccgatg aagcacaatt cgtcctgact gcctcgggta ctctcacatc taagtcggtt
    23521 gagttgaacc taacgaccca gtttgacgta caggaccgag cgagaccttt tgggattggg
    23581 cgtaatgtct actttgctag tccgaggtcc agcttcacgt ccatccacag gtactacgct
    23641 gtgcaggatg tcagttccgt taagaatgct gaggacatta catcacacgt tcctaactac
    23701 atccctaatg gtgtgttcag tatttgcgga agtggtacgg aaaacttctg ttcggtacta
    23761 tctcacgggg accctagtaa aatcttcatg tacaaattcc tgtacctgaa cgaagagtta
    23821 aggcaacagt cgtggtctca ttgggacttt ggggaaaacg tacaggttct agcttgtcag
    23881 agtatcagct cagatatgta tgtgattctt cgcaatgagt tcaatacgtt cctagctaga
    23941 atctctttca ctaagaacgc cattgactta cagggagaac cctatcgtgc ctttatggac
    24001 atgaagattc gatacacgat tcctagtgga acatacaacg atgacacatt cactacctct
    24061 attcatattc caacaattta tggtgcaaac ttcgggaggg gcaaaatcac tgtattggag
    24121 cctgatggta agataaccgt gtttgagcaa cctacggctg ggtggaatag cgacccttgg
    24181 ctgagactca gcggtaactt ggagggacgc atggtgtaca ttgggttcaa cattaacttc
    24241 gtatatgagt tctctaagtt cctcatcaag cagactgccg acgacgggtc tacctccacg
    24301 gaagacattg ggcgcttaca gttacgccga gcgtgggtta actacgagaa ctctggtacg
    24361 tttgacattt atgttgagaa ccaatcgtct aactggaagt acacaatggc tggtgcccga
    24421 ttaggctcta acactctgag ggctgggaga ctgaacttag ggaccggaca atatcgattc
    24481 cctgtggttg gtaacgccaa gttcaacact gtatacatct tgtcagatga gactacccct
    24541 ctgaacatca ttaggtgtgg ctgggaaggt aactacttac ggagaagttc cggtatttaa
    24601 ttaaatattc tccctgtggt ggctcgaaat taatacgact cactataggg agaacaatac
    24661 gactacggga gggttttctt atgatgacta taagacctac taaaagtaca gactttgagg
    24721 tattcactcc ggctcaccat gacattcttg aagctaaggc tgctggtatt gagccgagtt
    24781 tccctgatgc ttccgagtgt gtcacgttga gcctctatgg gttccctcta gctatcggtg
    24841 gtaactgcgg ggaccagtgc tggttcgtta cgagcgacca agtgtggcga cttagtggaa
    24901 aggctaagcg aaagttccgt aagttaatca tggagtatcg cgataagatg cttgagaagt
    24961 atgatactct ttggaattac gtatgggtag gcaatacgtc ccacattcgt ttcctcaaga
    25021 ctatcggtgc ggtattccat gaagagtaca cacgagatgg tcaatttcag ttatttacaa
    25081 tcacgaaagg aggataacca tatgtgttgg gcagccgcaa tacctatcgc tatatctggc
    25141 gctcaggcta tcagtggtca gaacgctcag gccaaaatga ttgccgctca gaccgctgct
    25201 ggtcgtcgtc aagctatgga aatcatgagg cagacgaaca tccagaatgc tgacctatcg
    25261 ttgcaagctc gaagtaaact tgaggaagcg tccgccgagt tgacctcaca gaacatgcag
    25321 aaggtccaag ctattgggtc tatccgagcg gctatcggag agagtatgct tgaaggttcc
    25381 tcaatggacc gcattaagcg agtcacagaa ggacagttca ttcgggaagc caatatggta
    25441 actgagaact atcgccgtga ctaccaagca atcttcgcac agcaacttgg tggtactcaa
    25501 agtgctgcaa gtcagattga cgaaatctat aagagcgaac agaaacagaa gagtaagcta
    25561 cagatggttc tggacccact ggctatcatg gggtcttccg ctgcgagtgc ttacgcatcc
    25621 ggtgcgttcg actctaagtc cacaactaag gcacctattg ttgccgctaa aggaaccaag
    25681 acggggaggt aatgagctat gagtaaaatt gaatctgccc ttcaagcggc acaaccggga
    25741 ctctctcggt tacgtggtgg tgctggaggt atgggctatc gtgcagcaac cactcaggcc
    25801 gaacagccaa ggtcaagcct attggacacc attggtcggt tcgctaaggc tggtgccgat
    25861 atgtataccg ctaaggaaca acgagcacga gacctagctg atgaacgctc taacgagatt
    25921 atccgtaagc tgacccctga gcaacgtcga gaagctctca acaacgggac ccttctgtat
    25981 caggatgacc catacgctat ggaagcactc cgagtcaaga ctggtcgtaa cgctgcgtat
    26041 cttgtggacg atgacgttat gcagaagata aaagagggtg tcttccgtac tcgcgaagag
    26101 atggaagagt atcgccatag tcgccttcaa gagggcgcta aggtatacgc tgagcagttc
    26161 ggcatcgacc ctgaggacgt tgattatcag cgtggtttca acggggacat taccgagcgt
    26221 aacatctcgc tgtatggtgc gcatgataac ttcttgagcc agcaagctca gaagggcgct
    26281 atcatgaaca gccgagtgga actcaacggt gtccttcaag accctgatat gctgcgtcgt
    26341 ccagactctg ctgacttctt tgagaagtat atcgacaacg gtctggttac tggcgcaatc
    26401 ccatctgatg ctcaagccac acagcttata agccaagcgt tcagtgacgc ttctagccgt
    26461 gctggtggtg ctgacttcct gatgcgagtc ggtgacaaga aggtaacact taacggagcc
    26521 actacgactt accgagagtt gattggtgag gaacagtgga acgctctcat ggtcacagca
    26581 caacgttctc agtttgagac tgacgcgaag ctgaacgagc agtatcgctt gaagattaac
    26641 tctgcgctga accaagagga cccaaggaca gcttgggaga tgcttcaagg tatcaaggct
    26701 gaactagata aggtccaacc tgatgagcag atgacaccac aacgtgagtg gctaatctcc
    26761 gcacaggaac aagttcagaa tcagatgaac gcatggacga aagctcaggc caaggctctg
    26821 gacgattcca tgaagtcaat gaacaaactt gacgtaatcg acaagcaatt ccagaagcga
    26881 atcaacggtg agtgggtctc aacggatttt aaggatatgc cagtcaacga gaacactggt
    26941 gagttcaagc atagcgatat ggttaactac gccaataaga agctcgctga gattgacagt
    27001 atggacattc cagacggtgc caaggatgct atgaagttga agtaccttca agcggactct
    27061 aaggacggag cattccgtac agccatcgga accatggtca ctgacgctgg tcaagagtgg
    27121 tctgccgctg tgattaacgg taagttacca gaacgaaccc cagctatgga tgctctgcgc
    27181 agaatccgca atgctgaccc tcagttgatt gctgcgctat acccagacca agctgagcta
    27241 ttcctgacga tggatatgat ggacaagcag ggtattgacc ctcaggttat tcttgatgcc
    27301 gaccgactga ctgttaagcg gtccaaagag caacgctttg aggatgataa agcattcgag
    27361 tctgcactga atgcatctaa ggctcctgag attgcccgta tgccagcgtc actgcgcgaa
    27421 tctgcacgta agatttatga ctccgttaag tatcgctcgg ggaacgaaag catggctatg
    27481 gagcagatga ccaagttcct taaggaatct acctacacgt tcactggtga tgatgttgac
    27541 ggtgataccg ttggtgtgat tcctaagaat atgatgcagg ttaactctga cccgaaatca
    27601 tgggagcaag gtcgggatat tctggaggaa gcacgtaagg gaatcattgc gagcaaccct
    27661 tggataacca ataagcaact gaccatgtat tctcaaggtg actccattta ccttatggac
    27721 accacaggtc aagtcagagt ccgatacgac aaagagttac tctcgaaggt ctggagtgag
    27781 aaccagaaga aactcgaaga gaaagctcgt gagaaggctc tggctgatgt gaacaagcga
    27841 gcacctatag ttgccgctac gaaggcccgt gaagctgctg ctaaacgagt ccgagagaaa
    27901 cgtaaacaga ctcctaagtt catctacgga cgtaaggagt aactaaaggc tacataagga
    27961 ggccctaaat ggataagtac gataagaacg taccaagtga ttatgatggt ctgttccaaa
    28021 aggctgctga tgccaacggg gtctcttatg accttttacg taaagtcgct tggacagaat
    28081 cacgatttgt gcctacagca aaatctaaga ctggaccatt aggcatgatg caatttacca
    28141 aggcaaccgc taaggccctc ggtctgcgag ttaccgatgg tccagacgac gaccgactga
    28201 accctgagtt agctattaat gctgccgcta agcaacttgc aggtctggta gggaagtttg
    28261 atggcgatga actcaaagct gcccttgcgt acaaccaagg cgagggacgc ttgggtaatc
    28321 cacaacttga ggcgtactct aagggagact tcgcatcaat ctctgaggag ggacgtaact
    28381 acatgcgtaa ccttctggat gttgctaagt cacctatggc tggacagttg gaaacttttg
    28441 gtggcataac cccaaagggt aaaggcattc cggctgaggt aggattggct ggaattggtc
    28501 acaagcagaa agtaacacag gaacttcctg agtccacaag ttttgacgtt aagggtatcg
    28561 aacaggaggc tacggcgaaa ccattcgcca aggacttttg ggagacccac ggagaaacac
    28621 ttgacgagta caacagtcgt tcaaccttct tcggattcaa aaatgctgcc gaagctgaac
    28681 tctccaactc agtcgctggg atggctttcc gtgctggtcg tctcgataat ggttttgatg
    28741 tgtttaaaga caccattacg ccgactcgct ggaactctca catctggact ccagaggagt
    28801 tagagaagat tcgaacagag gttaagaacc ctgcgtacat caacgttgta actggtggtt
    28861 cccctgagaa cctcgatgac ctcattaaat tggctaacga gaactttgag aatgactccc
    28921 gcgctgccga ggctggccta ggtgccaaac tgagtgctgg tattattggt gctggtgtgg
    28981 acccgcttag ctatgttcct atggtcggtg tcactggtaa gggctttaag ttaatcaata
    29041 aggctcttgt agttggtgcc gaaagtgctg ctctgaacgt tgcatccgaa ggtctccgta
    29101 cctccgtagc tggtggtgac gcagactatg cgggtgctgc cttaggtggc tttgtgtttg
    29161 gcgcaggcat gtctgcaatc agtgacgctg tagctgctgg actgaaacgc agtaaaccag
    29221 aagctgagtt cgacaatgag ttcatcggtc ctatgatgcg attggaagcc cgtgagacag
    29281 cacgaaacgc caactctgcg gacctctctc ggatgaacac tgagaacatg aagtttgaag
    29341 gtgaacataa tggtgtccct tatgaggact taccaacaga gagaggtgcc gtggtgttac
    29401 atgatggctc cgttctaagt gcaagcaacc caatcaaccc taagactcta aaagagttct
    29461 ccgaggttga ccctgagaag gctgcgcgag gaatcaaact ggctgggttc accgagattg
    29521 gcttgaagac cttggggtct gacgatgctg acatccgtag agtggctatc gacctcgttc
    29581 gctctcctac tggtatgcag tctggtgcct caggtaagtt cggtgcaaca gcttctgaca
    29641 tccatgagag acttcatggt actgaccagc gtacttataa tgacttgtac aaagcaatgt
    29701 ctgacgctat gaaagaccct gagttctcta ctggcggcgc taagatgtcc cgtgaagaaa
    29761 ctcgatacac tatctaccgt agagcggcac tagctattga gcgtccagaa ctacagaagg
    29821 cactcactcc gtctgagaga atcgttatgg acatcattaa gcgtcacttt gacaccaagc
    29881 gtgaacttat ggaaaaccca gcaatattcg gtaacacaaa ggctgtgagt atcttccctg
    29941 agagtcgcca caaaggtact tacgttcctc acgtatatga ccgtcatgcc aaggcgctga
    30001 tgattcaacg ctacggtgcc gaaggtttgc aggaagggat tgcccgctca tggatgaaca
    30061 gctacgtctc cagacctgag gtcaaggcca gagtcgatga gatgcttaag gaattacacg
    30121 gggtgaagga agtaacacca gagatggtag agaagtacgc tatggataag gcttatggta
    30181 tctcccactc agaccagttc accaacagtt ccataataga agagaacatt gagggcttag
    30241 taggtatcga gaataactca ttccttgagg cacgtaactt gtttgattcg gacctatcca
    30301 tcactatgcc agacggacag caattctcag tgaatgacct aagggacttc gatatgttcc
    30361 gcatcatgcc agcgtatgac cgccgtgtca atggtgacat cgccatcatg gggtctactg
    30421 gtaaaaccac taaggaactt aaggatgaga ttttggctct caaagcgaaa gctgagggag
    30481 acggtaagaa gactggcgag gtacatgctt taatggatac cgttaagatt cttactggtc
    30541 gtgctagacg caatcaggac actgtgtggg aaacctcact gcgtgccatc aatgacctag
    30601 ggttcttcgc taagaacgcc tacatgggtg ctcagaacat tacggagatt gcggggatga
    30661 ttgtcactgg taacgttcgt gctctagggc atggtatccc aattctgcgt gatacactct
    30721 acaagtctaa accagtttca gctaaggaac tcaaggaact ccatgcgtct ctgttcggga
    30781 aggaggtgga ccagttgatt cggcctaaac gtgctgacat tgtgcagcgc ctaagggaag
    30841 caactgatac cggacctgcc gtggcgaaca tcgtagggac cttgaagtat tcaacacagg
    30901 aactggctgc tcgctctccg tggactaagc tactgaacgg aaccactaac taccttctgg
    30961 atgctgcgcg tcaaggtatg cttggggatg ttattagtgc caccctaaca ggtaagacta
    31021 cccgctggga gaaagaaggc ttccttcgtg gtgcctccgt aactcctgag cagatggctg
    31081 gcatcaagtc tctcatcaag gaacatatgg tacgcggtga ggacgggaag tttaccgtta
    31141 aggacaagca agcgttctct atggacccac gggctatgga cttatggaga ctggctgaca
    31201 aggtagctga tgaggcaatg ctgcgtccac ataaggtgtc cttacaggat tcccatgcgt
    31261 tcggagcact aggtaagatg gttatgcagt ttaagtcttt cactatcaag tcccttaact
    31321 ctaagttcct gcgaaccttc tatgatggat acaagaacaa ccgagcgatt gacgctgcgc
    31381 tgagcatcat cacctctatg ggtctcgctg gtggtttcta tgctatggct gcacacgtca
    31441 aagcatacgc tctgcctaag gagaaacgta aggagtactt ggagcgtgca ctggacccaa
    31501 ccatgattgc ccacgctgcg ttatctcgta gttctcaatt gggtgctcct ttggctatgg
    31561 ttgacctagt tggtggtgtt ttagggttcg agtcctccaa gatggctcgc tctacgattc
    31621 tacctaagga caccgtgaag gaacgtgacc caaacaaacc gtacacctct agagaggtaa
    31681 tgggcgctat gggttcaaac cttctggaac agatgccttc ggctggcttt gtggctaacg
    31741 taggggctac cttaatgaat gctgctggcg tggtcaactc acctaataaa gcaaccgagc
    31801 aggacttcat gactggtctt atgaactcca caaaagagtt agtaccgaac gacccattga
    31861 ctcaacagct tgtgttgaag atttatgagg cgaacggtgt taacttgagg gagcgtagga
    31921 aataatacga ctcactatag ggagaggcga aataatcttc tccctgtagt ctcttagatt
    31981 tactttaagg aggtcaaatg gctaacgtaa ttaaaaccgt tttgacttac cagttagatg
    32041 gctccaatcg tgattttaat atcccgtttg agtatctagc ccgtaagttc gtagtggtaa
    32101 ctcttattgg tgtagaccga aaggtcctta cgattaatac agactatcgc tttgctacac
    32161 gtactactat ctctctgaca aaggcttggg gtccagccga tggctacacg accatcgagt
    32221 tacgtcgagt aacctccact accgaccgat tggttgactt tacggatggt tcaatcctcc
    32281 gcgcgtatga ccttaacgtc gctcagattc aaacgatgca cgtagcggaa gaggcccgtg
    32341 acctcactac ggatactatc ggtgtcaata acgatggtca cttggatgct cgtggtcgtc
    32401 gaattgtgaa cctagcgaac gccgtggatg accgcgatgc tgttccgttt ggtcaactaa
    32461 agaccatgaa ccagaactca tggcaagcac gtaatgaagc cttacagttc cgtaatgagg
    32521 ctgagacttt cagaaaccaa gcggagggct ttaagaacga gtccagtacc aacgctacga
    32581 acacaaagca gtggcgcgat gagaccaagg gtttccgaga cgaagccaag cggttcaaga
    32641 atacggctgg tcaatacgct acatctgctg ggaactctgc ttccgctgcg catcaatctg
    32701 aggtaaacgc tgagaactct gccacagcat ccgctaactc tgctcatttg gcagaacagc
    32761 aagcagaccg tgcggaacgt gaggcagaca agctggaaaa ttacaatgga ttggctggtg
    32821 caattgataa ggtagatgga accaatgtgt actggaaagg aaatattcac gctaacgggc
    32881 gcctttacat gaccacaaac ggttttgact gtggccagta tcaacagttc tttggtggtg
    32941 tcactaatcg ttactctgtc atggagtggg gagatgagaa cggatggctg atgtatgttc
    33001 aacgtagaga gtggacaaca gcgataggcg gtaacatcca gttagtagta aacggacaga
    33061 tcatcaccca aggtggagcc atgaccggtc agctaaaatt gcagaatggg catgttcttc
    33121 aattagagtc cgcatccgac aaggcgcact atattctatc taaagatggt aacaggaata
    33181 actggtacat tggtagaggg tcagataaca acaatgactg taccttccac tcctatgtac
    33241 atggtacgac cttaacactc aagcaggact atgcagtagt taacaaacac ttccacgtag
    33301 gtcaggccgt tgtggccact gatggtaata ttcaaggtac taagtgggga ggtaaatggc
    33361 tggatgctta cctacgtgac agcttcgttg cgaagtccaa ggcgtggact caggtgtggt
    33421 ctggtagtgc tggcggtggg gtaagtgtga ctgtttcaca ggatctccgc ttccgcaata
    33481 tctggattaa gtgtgccaac aactcttgga acttcttccg tactggcccc gatggaatct
    33541 acttcatagc ctctgatggt ggatggttac gattccaaat acactccaac ggtctcggat
    33601 tcaagaatat tgcagacagt cgttcagtac ctaatgcaat catggtggag aacgagtaat
    33661 tggtaaatca caaggaaaga cgtgtagtcc acggatggac tctcaaggag gtacaagatg
    33721 gaaaaggata agagccttat tacattctta gagatgttgg acactgcgat ggctcagcgt
    33781 atgcttgcgg acctttcgga ccatgagcgt cgctctccgc aactctataa tgctattaac
    33841 aaactgttag accgccacaa gttccagatt ggtaagttgc agccggatgt tcacatctta
    33901 ggtggccttg ctggtgctct tgaagagtac aaagagaaag tcggtgataa cggtcttacg
    33961 gatgatgata tttacacatt acagtgactc gagaggatcc actacagata gtggtcttta
    34021 tggatgtcat tgtctatacg agatgctcct acgtgaaatc tgaaagttaa cgggaggcat
    34081 tatgctagaa tttttacgta agctaatccc ttgggttctc gctgggatgc tattcgggtt
    34141 aggatggcat ctagggtcag actcaatgga cgctaaatgg aaacaggagg tacacaatga
    34201 gtacgttaag agagttgagg ctgcgaagag cactcaaaga gcaatcgatg cggtatctgc
    34261 taagtatcaa gaagaccttg ccgcgctgga agggagcact gataggatta tttctgattt
    34321 gcgtagcgac aataagcggt tgcgcgtcag agtcaaaact accggaacct ccgatggtca
    34381 gtgtggattc gagcctgatg gtcgagccga acttgacgac cgagatgcta aacgtattct
    34441 cgcagtgacc cagaagggtg acgcatggat tcgtgcgtta caggatacta ttcgtgaact
    34501 gcaacgtaag taggaaatca agtaaggagg caatgtgtct actcaatcca atcgtaatgc
    34561 gctcgtagtg gcgcaactga aaggagactt cgtggcgttc ctattcgtct tatggaaggc
    34621 gctaaaccta ccggtgccca ctaagtgtca gattgacatg gctaaggtgc tggcgaatgg
    34681 agacaacaag aagttcatct tacaggcttt ccgtggtatc ggtaagtcgt tcatcacatg
    34741 tgcgttcgtt gtgtggtcct tatggagaga ccctcagttg aagatactta tcgtatcagc
    34801 ctctaaggag cgtgcagacg ctaactccat ctttattaag aacatcattg acctgctgcc
    34861 attcctatct gagttaaagc caagacccgg acagcgtgac tcggtaatca gctttgatgt
    34921 aggcccagcc aatcctgacc actctcctag tgtgaaatca gtaggtatca ctggtcagtt
    34981 aactggtagc cgtgctgaca ttatcattgc ggatgacgtt gagattccgt ctaacagcgc
    35041 aactatgggt gcccgtgaga agctatggac tctggttcag gagttcgctg cgttacttaa
    35101 accgctgcct tcctctcgcg ttatctacct tggtacacct cagacagaga tgactctcta
    35161 taaggaactt gaggataacc gtgggtacac aaccattatc tggcctgctc tgtacccaag
    35221 gacacgtgaa gagaacctct attactcaca gcgtcttgct cctatgttac gcgctgagta
    35281 cgatgagaac cctgaggcac ttgctgggac tccaacagac ccagtgcgct ttgaccgtga
    35341 tgacctgcgc gagcgtgagt tggaatacgg taaggctggc tttacgctac agttcatgct
    35401 taaccctaac cttagtgatg ccgagaagta cccgctgagg cttcgtgacg ctatcgtagc
    35461 ggccttagac ttagagaagg ccccaatgca ttaccagtgg cttccgaacc gtcagaacat
    35521 cattgaggac cttcctaacg ttggccttaa gggtgatgac ctgcatacgt accacgattg
    35581 ttccaacaac tcaggtcagt accaacagaa gattctggtc attgacccta gtggtcgcgg
    35641 taaggacgaa acaggttacg ctgtgctgta cacactgaac ggttacatct accttatgga
    35701 agctggaggt ttccgtgatg gctactccga taagaccctt gagttactcg ctaagaaggc
    35761 aaagcaatgg ggagtccaaa cggttgtcta cgagagtaac ttcggtgacg gtatgttcgg
    35821 taaggtattc agtcctatcc ttcttaaaca ccacaactgt gcgatggaag agattcgtgc
    35881 ccgtggtatg aaagagatgc gtatttgcga tacccttgag ccagtcatgc agactcaccg
    35941 ccttgtaatt cgtgatgagg tcattagggc cgactaccag tccgctcgtg acgtagacgg
    36001 taagcatgac gttaagtact cgttgttcta ccagatgacc cgtatcactc gtgagaaagg
    36061 cgctctggct catgatgacc gattggatgc ccttgcgtta ggcattgagt atctccgtga
    36121 gtccatgcag ttggattccg ttaaggtcga gggtgaagta cttgctgact tccttgagga
    36181 acacatgatg cgtcctacgg ttgctgctac gcatatcatt gagatgtctg tgggaggagt
    36241 tgatgtgtac tctgaggacg atgagggtta cggtacgtct ttcattgagt ggtgatttat
    36301 gcattaggac tgcataggga tgcactatag accacggatg gtcagttctt taagttactg
    36361 aaaagacacg ataaattaat acgactcact atagggagag gagggacgaa aggttactat
    36421 atagatactg aatgaatact tatagagtgc ataaagtatg cataatggtg tacctagagt
    36481 gacctctaag aatggtgatt atattgtatt agtatcacct taacttaagg accaacataa
    36541 agggaggaga ctcatgttcc gcttattgtt gaacctactg cggcatagag tcacctaccg
    36601 atttcttgtg gtactttgtg ctgaccttgg gtacgcatct cttactggag acctcagttc
    36661 actggagtct gtcgtttgct ctatactcac ttgtagcgat tagggtcttc ctgaccgact
    36721 gatggctcac cgagggattc agcggtatga ttgcatcaca ccacttcatc cctatagagt
    36781 caagtcctga ggtataccca taaagagcct ctaatggtct atcctaaggt ctatacctaa
    36841 agataggcca tcctatcagt gtcacctaaa gagggtctta gagagggcct atggagttcc
    36901 tatagggtcc tttaaaatat accataaaaa tctgagtgac ta
//