LOCUS       ML004966               19314 bp    DNA     linear   CON 10-OCT-2018
DEFINITION  Rozella allomycis CSF55 unplaced genomic scaffold
            ROZALSC1scaffold_62, whole genome shotgun sequence.
ACCESSION   ML004966 QUVT01000000
VERSION     ML004966.1
DBLINK      BioProject: PRJNA421584
            BioSample: SAMN09741938
KEYWORDS    WGS.
SOURCE      Rozella allomycis CSF55
  ORGANISM  Rozella allomycis CSF55
            Eukaryota; Fungi; Fungi incertae sedis; Cryptomycota; Cryptomycota
            incertae sedis; Rozella.
REFERENCE   1  (bases 1 to 19314)
  AUTHORS   Ahrendt,S.R., Quandt,C.A., Ciobanu,D., Clum,A., Salamov,A.,
            Andreopoulos,B., Cheng,J.-F., Woyke,T., Pelin,A., Henrissat,B.,
            Reynolds,N., Benny,G.L., Smith,M.E., James,T.Y. and Grigoriev,I.V.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Leveraging single-cell genomics to expand the Fungal Tree of Life
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19314)
  AUTHORS   Ahrendt,S.R., Cantor,M.N. and Hua,S.X.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2018) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://genome.jgi.doe.gov/Rozal_SC1
            JGI Project ID: 1000565
            The DNA was provided by Timothy James(tyjames@umich.edu)
            The strain is available from Timothy James
            Contacts: Timothy Y. James (tyjames@umich.edu)
            Assembly and annotation done by JGI.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            
            This submission is a co-assembly of two 100 cell Illumina
            libraries.  The amplification method for both libraries was MDA.
            
            ##Metadata-START##
            Organism Display Name :: Rozella allomycis CSF55 single-cell
            GOLD Stamp ID         :: Gp0009558
            ##Metadata-END##
            
            ##Genome-Assembly-Data-START##
            Assembly Method           :: SPAdes v. 3.6.0
            Assembly Name             :: Rozal_SC1
            Single-cell Amplification :: MDA
            Genome Coverage           :: 100x
            Sequencing Technology     :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19314
                     /organism="Rozella allomycis CSF55"
                     /mol_type="genomic DNA"
                     /submitter_seqid="ROZALSC1scaffold_62"
                     /strain="CSF55"
                     /isolation_source="co-assembly of two 100 cell libraries
                     amplified by MDA"
                     /db_xref="taxon:988480"
                     /chromosome="Unknown"
     gene            <1036..>2554
                     /locus_tag="ROZALSC1DRAFT_20613"
     mRNA            join(<1036..1125,1266..1393,1491..1672,2179..2396,
                     2438..>2554)
                     /locus_tag="ROZALSC1DRAFT_20613"
                     /product="hypothetical protein"
     CDS             join(1036..1125,1266..1393,1491..1672,2179..2396,
                     2438..2554)
                     /locus_tag="ROZALSC1DRAFT_20613"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP21312.1"
                     /db_xref="JGIDB:Rozal_SC1_20613"
                     /translation="MLVKCNINDEEDHVLIEGNYEENIFKSFARLRLHQIWFRIGESN
                     SGLCGESTKSPSRWVPDLVESQLLLIRLKPLPRIHHSQSHYHIIINLQQAHNQNYYCR
                     VKNLFIRWFCFGSVSVVFEIPVAIREAWNWMITDSVSNIASTSAPAYGKQASDVWKTF
                     TLEENSTKAKSLLRCLSIIQRRRRISLRTHYAAQGDTTSNAKECKLNEFCVHARVFCR
                     ADCRICGQSFAMIKSKGFRELRASPS"
     gene            complement(2821..2892)
                     /locus_tag="ROZALSC1DRAFT_t18"
     tRNA            complement(2821..2892)
                     /locus_tag="ROZALSC1DRAFT_t18"
                     /product="tRNA-Gln"
                     /note="Anticodon: TTG"
                     /anticodon=(pos:complement(2857..2859),aa:Gln,seq:ttg)
     gene            complement(<2919..>4306)
                     /locus_tag="ROZALSC1DRAFT_27265"
     mRNA            complement(join(<2919..3196,3225..3383,3413..3556,
                     3586..3716,3744..3901,3941..4237,4265..>4306))
                     /locus_tag="ROZALSC1DRAFT_27265"
                     /product="RCC1/BLIP-II"
     CDS             complement(join(2919..3196,3225..3383,3413..3556,
                     3586..3716,3744..3901,3941..4237,4265..4306))
                     /locus_tag="ROZALSC1DRAFT_27265"
                     /codon_start=1
                     /product="RCC1/BLIP-II"
                     /protein_id="RKP21313.1"
                     /db_xref="InterPro:IPR000408"
                     /db_xref="JGIDB:Rozal_SC1_27265"
                     /translation="MQTSRIYLWGHSGSVNNEARSIKEPKELECFESKGLTSVSCGAL
                     HTSFCFDEKELYMMGHGESGQLGNGLGTSSFEIPVHVKLPISSEGKIISVSCGYRHTI
                     VLTSEGVYGFGGQLGLGYEEDLIPNKALVPRKITFLDPYDVVAVACGSRHTLFLLSNG
                     QILGTGYNAHGQLGLNSRRDYNAPAVIEYLENANIVKVTAGELHSAVLTTCGELYVFG
                     LGRAVGFAEDCLVPTKIDFGTHEIVRDVQCGASHTLVLTKQGDVYSFGAGSHGQLGIG
                     KVCVKPQRPQLIKDLVGKYVTDISAGVRFSIAATADGDVYGWGENVDNVFCVGKENEI
                     QVKPKKISHFDKSKVVGIECGGWHVIAIVKGEKDPKGTDYACFLIAHVKDILNEDPKL
                     SLALGDRRKC"
     gene            complement(<4376..>6537)
                     /locus_tag="ROZALSC1DRAFT_27266"
     mRNA            complement(join(<4376..4508,4540..4749,4770..4803,
                     4867..4930,4958..5086,5113..5232,5262..5380,5409..6019,
                     6048..6172,6202..6250,6311..>6537))
                     /locus_tag="ROZALSC1DRAFT_27266"
                     /product="kinesin-domain-containing protein"
     CDS             complement(join(4376..4508,4540..4749,4770..4803,
                     4867..4930,4958..5086,5113..5232,5262..5380,5409..6019,
                     6048..6172,6202..6250,6311..6537))
                     /locus_tag="ROZALSC1DRAFT_27266"
                     /note="GO_component: GO:5875 - microtubule associated
                     complex;
                     GO_function: GO:3777; ATP binding - microtubule motor
                     activity [GO Ref 5524];
                     GO_process: GO:7018 - microtubule-based movement"
                     /codon_start=1
                     /product="kinesin-domain-containing protein"
                     /protein_id="RKP21314.1"
                     /db_xref="InterPro:IPR001752"
                     /db_xref="JGIDB:Rozal_SC1_27266"
                     /translation="MSAKYPIPSKPTIRHFPYEQFSHDTSRSYSAETGDQNWNFDGSS
                     TTSSSSAFIDRSIVQSGKDEVNLESQIRVVVRDDRYSVLALDDGQTIQVTIPEGPPKG
                     LTFHKVHTETCTQKQLFDESGVINLLDRALEGYSVTIFAYGQTSSGKTFTMTGPDIDE
                     PLPSDQQGLIPRALHYIYAKIAQNKQAHYTVRSSYLEIYNEQVRDLLNIGGALTLPVR
                     WKQDKGFYVENIFVVECDVLDDNLAVLEEGTYINAGIIKGLRNRKTGSHRLNENSSRS
                     HSIMSVYLEIENIDPDDNRLIKRTGKISFVDLAGSEKVKESQASGGTFNEMLNINKSL
                     LTLGNCISALSDPKKKNGHIPFRDSKLTKLLADSLGGNGYTLMIACISPSSYNLNESL
                     KTLRYANRAKNIRNKPVIQMDPRDELIMQYKRENKILKSENLYLRNLSGLGTADLSHL
                     HGDPETVSKLTATMLNLKPSFLPTVPNRNTNELEFTKKENVEWSNLQSQKNMTEIRLK
                     DVMEENEMLSLKLERLEKVFLGKETNLSPESESSTHDSNTKPDMLGNKPVHYKKGERD
                     DQKSSTTEKAPSLSNVVMSKQKLQKELQYLDNEIQSLTKK"
     gene            <6713..>7467
                     /locus_tag="ROZALSC1DRAFT_27267"
     mRNA            join(<6713..6827,6856..7243,7270..7387,7411..>7467)
                     /locus_tag="ROZALSC1DRAFT_27267"
                     /product="hypothetical protein"
     CDS             join(6713..6827,6856..7243,7270..7387,7411..7467)
                     /locus_tag="ROZALSC1DRAFT_27267"
                     /note="GO_component: GO:5667 - transcription factor
                     complex;
                     GO_function: GO:3700 - transcription factor activity;
                     GO_process: GO:6355 - regulation of transcription,
                     DNA-dependent"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP21315.1"
                     /db_xref="InterPro:IPR003316"
                     /db_xref="JGIDB:Rozal_SC1_27267"
                     /translation="MTLHSPALGVTNPNRKRKLNSDICITSDLNSTTSSTGQGSRTES
                     SLGSLSKKFLNLIRQSPDGLVDLNQAAIQLGVAKRRIYDITNVMEGIGTLDKVGKNSV
                     KYRFDIVKCVNRGVNNEELPEEQENELKRFQQRIKELQEEESFLDDQKFLIERSFSTQ
                     EFNDDYKNRLAFVTHSDIRSIDCFSKDTLFAVKAPKGCSLEVPDPDVKEEFPGHRKIR
                     NSSLQSR"
     gene            <8183..>9390
                     /locus_tag="ROZALSC1DRAFT_11571"
     mRNA            join(<8183..8246,8273..8343,8373..8501,8531..8724,
                     8756..8843,8870..8945,8973..9100,9127..9219,9250..>9390)
                     /locus_tag="ROZALSC1DRAFT_11571"
                     /product="RabGAP/TBC"
     CDS             join(<8183..8246,8273..8343,8373..8501,8531..8724,
                     8756..8843,8870..8945,8973..9100,9127..9219,9250..9390)
                     /locus_tag="ROZALSC1DRAFT_11571"
                     /note="GO_component: GO:5622 - intracellular;
                     GO_function: GO:5097 - Rab GTPase activator activity;
                     GO_process: GO:32313 - regulation of Rab GTPase activity"
                     /codon_start=1
                     /product="RabGAP/TBC"
                     /protein_id="RKP21316.1"
                     /db_xref="InterPro:IPR000195"
                     /db_xref="JGIDB:Rozal_SC1_11571"
                     /translation="NSGFSRSDKFKSILLQPNVDIIQLKKLAWNGIPNEFRAEIWQIL
                     LGLLPVNSERRKSTLERKRKEYIENATTLFAKGVEGLDHTTYHQIHIDMPRTNPEVEL
                     FQRKVIQEALERILYCWAIRHPASGYVQGINDLVTPFFYVFISCNNNQNIKKPFEIDE
                     FQLQNVEADSYWCLNKLLDGIQDNYTFSQPGIQRQVQKLKELMLRIDKQLYDHFHYQG
                     VEFIQFAFRWVNCLLLREIPIHLVIRMWDTYLCEENGFSEFHIYVCASFLHCFREKLL
                     TMDFQEIFLFLQNGLPTKEWSEKEIEIILSQAFMWKSLFQNAQSHLSKNAK"
     gene            <9520..>10503
                     /locus_tag="ROZALSC1DRAFT_27270"
     mRNA            <9520..>10503
                     /locus_tag="ROZALSC1DRAFT_27270"
                     /product="hypothetical protein"
     CDS             9520..10503
                     /locus_tag="ROZALSC1DRAFT_27270"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP21317.1"
                     /db_xref="JGIDB:Rozal_SC1_27270"
                     /translation="MMPYPNTKPLDIQPRIYRSDWKDVKNNVKAMDRLFKAKFENYII
                     NENNVISISAGIRRVMNEYSIGQISNGLRWLIKEWTLGTITTFILKTFIEDFWEIKED
                     ENQLKNKRRVSLTAIISELENETATSPSNSQYEPFLHTSLDEIYLNNTENSFKMMGIS
                     YKHSLRELERRIKLITSMTDGWYTHFIAELIKAILSEFKRSDQKRFFMERFVEGWTFQ
                     MLSELFNYLGSYLEWETKKAMLKQFATKKSSIDKFPKIPSYKFTVSNITSENRRPSRN
                     SRDFILMQRRRSSQTMIPILTTVNENNQLSPMEVDLPQTAEKPTHTSSLIG"
     gene            <10738..>13337
                     /locus_tag="ROZALSC1DRAFT_27271"
     mRNA            join(<10738..10775,10805..11093,11122..11361,11390..11532,
                     11563..11655,11686..11823,11850..12126,12156..12469,
                     12496..12936,12965..>13337)
                     /locus_tag="ROZALSC1DRAFT_27271"
                     /product="hypothetical protein"
     CDS             join(10738..10775,10805..11093,11122..11361,11390..11532,
                     11563..11655,11686..11823,11850..12126,12156..12469,
                     12496..12936,12965..13337)
                     /locus_tag="ROZALSC1DRAFT_27271"
                     /note="GO_component: GO:5634; cytoplasm - nucleus [GO Ref
                     5737];
                     GO_function: GO:5525 - GTP binding"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP21318.1"
                     /db_xref="InterPro:IPR006762"
                     /db_xref="JGIDB:Rozal_SC1_27271"
                     /translation="MQNAQILILGLKRSGKSSILRVIFHDATPPETLFLETTSSIRTE
                     SITSSLIPFSVIDYPEFWNDENSREILKKMQVLLFVIDAQDELLDPLSRLYNIVLSLN
                     SLNSNLKIHVFIHKIDCLSEAYQREIRQDIQQRMSDEFEQAGVSDISIAYHLTSIYDN
                     SVFEAFSLVIQSVIPSLCSFENILDIFCANTSCDKVFLFDPGCKLYFATDSSPVDPLT
                     FDLCSDILDVVIDLTFIYSSEGSQSLNPIDRECTLALSNGYTVLFYQINKFLCLIAIM
                     SGDFNNLRPLLSYNLNLAKEAILEMHQKINSHHKFYSSDLTISVKFAMSINRPLLAID
                     VDEVLSMTHEAMIKFYNENYNANLELSDFTSYSYWQVWNVSLEESIEITRIFYNSEHF
                     DNVRPVPCAFEVLKALQFKFDLVVVTSRQETIKEKTIQFIERYYPGIFKGVYFGNHYL
                     SEEEKKNLVPKSKSTICQELGAFAIVDDSLAIALECAEIGMDVFLFDLEHSYMWNKTE
                     YHRLPSSIHRVLNWIQVADWLNVRHLALSRENLNSPKQIDENRIIASFVQTKYMTIKH
                     LKKRSMPNFDRIRSRVHVRLSGPLSNIITNQNGNEIKTQLWVEYLCRSHFRHHGGLEK
                     VERGLLVGNFVKSNLVEKEFKRYISEIETEYNEKNNYDLVLTLATEQKDDVINQMDQL
                     YSSKHLNIINKQKKPTYYGFLLNLPAFSGDEEEDIDTWHAELEELLFRKVSVWGSVMN
                     FQVFFLKYEGGQFETDHKFAKLSPDLAPELLYLSFWLQSTN"
     gene            complement(<13555..>14736)
                     /locus_tag="ROZALSC1DRAFT_20621"
     mRNA            complement(join(<13555..13824,13852..13980,14201..14293,
                     14321..14626,14656..>14736))
                     /locus_tag="ROZALSC1DRAFT_20621"
                     /product="hypothetical protein"
     CDS             complement(join(13555..13824,13852..13980,14201..14293,
                     14321..14626,14656..14736))
                     /locus_tag="ROZALSC1DRAFT_20621"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP21319.1"
                     /db_xref="JGIDB:Rozal_SC1_20621"
                     /translation="MRNLEEKLRTDFVNEKNELMENHERHISIMKEKINKEKETKLLE
                     EIEFMKKRYRRENEQSLLNFDSMKSKLILEFNEEKENLIASHKKHVENMQNFHRGQIE
                     ELQETIARCKLEGISSLEEHKRRSLGDMRQLEEKIKLEKDEWQSHLLIKLEKQFKEKE
                     MKEKINEFEKNCKLLQEANLETAKKCEQLEELKKEMDEKHNELSNELREKENMILQFN
                     RQRLELKEEISNLAFFVFKGMGFKEELSAKDTAFETLHKKFQEASRRFDEMQSLNKAE
                     LSRVNQEKVFYPINFE"
     gene            complement(<15393..>16737)
                     /locus_tag="ROZALSC1DRAFT_27273"
     mRNA            complement(join(<15393..15480,15569..15632,15660..16152,
                     16180..16345,16373..>16737))
                     /locus_tag="ROZALSC1DRAFT_27273"
                     /product="wd-repeat protein"
     CDS             complement(join(15393..15480,15569..15632,15660..16152,
                     16180..16345,16373..16737))
                     /locus_tag="ROZALSC1DRAFT_27273"
                     /codon_start=1
                     /product="wd-repeat protein"
                     /protein_id="RKP21320.1"
                     /db_xref="InterPro:IPR001680"
                     /db_xref="JGIDB:Rozal_SC1_27273"
                     /translation="MDKRKAEMQVSIDVKKPKVAETSIVKTFNSKGKQIQSIQVKRTS
                     GLKSPIMLLTGHESDILTTRFSPCGNYLASGSFDKTICKHFIISRIVLWNVYGECTNF
                     NVMRGQNGAILELDWSPNSERIFTACSDKTVGIYDVIVGKNIRKLKGHQNIINSVNCL
                     RREPHLIVSGSDDKMINIWDQRQKEPVSTFTQRFQITSVSFGQVGDLVFAGSLDNDIT
                     AWDIRNQNISYKLNGHIDTITGLRLSPDGMNILSASMDHSVRLWDVKPYASGNRCLKI
                     FEGAPHGHEKNLIRPSWSHDAQRVACGSADRTVLVWDVPSRKILYKLPGHKGCVNEVD
                     FHPEEPIIASCSADRQIFLGEIEPSYSESFKKSGRGPYDQKNRQCFKIFAVHRRKGQ"
     gene            <16997..>19008
                     /locus_tag="ROZALSC1DRAFT_27274"
     mRNA            join(<16997..17225,17258..17436,17551..17694,17721..17867,
                     17895..18104,18131..18339,18369..>19008)
                     /locus_tag="ROZALSC1DRAFT_27274"
                     /product="WD40 repeat-like protein"
     CDS             join(16997..17225,17258..17436,17551..17694,17721..17867,
                     17895..18104,18131..18339,18369..19008)
                     /locus_tag="ROZALSC1DRAFT_27274"
                     /codon_start=1
                     /product="WD40 repeat-like protein"
                     /protein_id="RKP21321.1"
                     /db_xref="InterPro:IPR001680"
                     /db_xref="InterPro:IPR010754"
                     /db_xref="JGIDB:Rozal_SC1_27274"
                     /translation="MSIPLVKLGYLFLRTISKPIASFMKSEAKRRPAFRSACIQVAES
                     YHQMEARMRSKLHDKAPERIRPLDEERAINVGANFIGEFIIFSVAGLVVVLESTRNKG
                     KVRDRKEFVDESIETLESQMNRLQAEISELKDLIRREDENTPSFDEMDLEEFSDSEIE
                     EIVESDHEVHEANMDIENADEETKVYLPGDKLEEGEELTFDSSAYEMYHELTPEWPCL
                     SFDIVSDSLGENRVLFPMSATIVAGSQASNACDNKLYVAKLSNLCKTKYDDDSDEESD
                     DDEEENNGEPILSVRTASLNAGVNRVRAMPQSTNEHRIVSAWTDDGKVRLFNIAEAME
                     SLENPTKDNMNKTPSNFGVIKGHKGEGFALDWSVRVPGRLLSGDVNGVICMTNVSPDQ
                     VANPDQFKGHGGSIEDLQWSPKENDVFASCSTDGTIKLWDVRSKNACILTAPVSSVDV
                     NVISWNSKEDYLLASGFDDGKFSIFDLRHFSMNAFDPVASFSWHKSPITSIEWHPTDE
                     TVLAVSGADDQLTLWDFSLEEDIEVAHDSMKDIPPQLLFIHQGQDNIKELHWHRQLPN
                     VIISTALNGFNIFKCSTNA"
CONTIG      join(QUVT01000109.1:1..19314)
//