LOCUS       ML002449               30912 bp    DNA     linear   CON 10-OCT-2018
DEFINITION  Dimargaris cristalligena strain RSA 468 unplaced genomic scaffold
            BJ085NODE_242, whole genome shotgun sequence.
ACCESSION   ML002449 QRFA01000000
VERSION     ML002449.1
DBLINK      BioProject: PRJNA346215
            BioSample: SAMN05877899
KEYWORDS    WGS.
SOURCE      Dimargaris cristalligena
  ORGANISM  Dimargaris cristalligena
            Eukaryota; Fungi; Fungi incertae sedis; Zoopagomycota;
            Kickxellomycotina; Dimargaritomycetes; Dimargaritales;
            Dimargaritaceae; Dimargaris.
REFERENCE   1  (bases 1 to 30912)
  AUTHORS   Ahrendt,S.R., Quandt,C.A., Ciobanu,D., Clum,A., Salamov,A.,
            Andreopoulos,B., Cheng,J.-F., Woyke,T., Pelin,A., Henrissat,B.,
            Reynolds,N., Benny,G.L., Smith,M.E., James,T.Y. and Grigoriev,I.V.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Leveraging single-cell genomics to expand the Fungal Tree of Life
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30912)
  AUTHORS   Ahrendt,S.R., Cantor,M.N. and Hua,S.X.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2018) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://genome.jgi.doe.gov/DimcrSC1
            JGI Project ID: 1072968
            The DNA was provided by Timothy James (tyjames@umich.edu)
            The strain is available from Timothy James
            Contacts: Timothy Y. James (tyjames@umich.edu)
            Assembly and annotation done by JGI.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##Metadata-START##
            Organism Display Name :: Dimargaris cristalligena RSA 468
                                     single-cell
            GOLD Stamp ID         :: Gp0047352
            ##Metadata-END##
            
            ##Genome-Assembly-Data-START##
            Assembly Method           :: SPAdes v. 3.5.0
            Assembly Name             :: DimcrSC1
            Single-cell Amplification :: MDA
            Genome Coverage           :: 100x
            Sequencing Technology     :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30912
                     /organism="Dimargaris cristalligena"
                     /mol_type="genomic DNA"
                     /submitter_seqid="BJ085NODE_242"
                     /strain="RSA 468"
                     /isolation_source="7 single cells amplifed by MDA"
                     /db_xref="taxon:215637"
                     /chromosome="Unknown"
     gene            <31..>309
                     /locus_tag="BJ085DRAFT_41563"
     mRNA            <31..>309
                     /locus_tag="BJ085DRAFT_41563"
                     /product="hypothetical protein"
     CDS             <31..309
                     /locus_tag="BJ085DRAFT_41563"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37764.1"
                     /db_xref="JGIDB:DimcrSC1_41563"
                     /translation="DQFLVRRAKKNKGSKSKNAEMAAPLNRRDIGEDQFLVRRAKKNK
                     GSKTKNAETAAPLNRRDIGEDQFLVRRSKKNKSNKKGKSAELPPAPTA"
     gene            complement(<2130..>4112)
                     /locus_tag="BJ085DRAFT_41564"
     mRNA            complement(<2130..>4112)
                     /locus_tag="BJ085DRAFT_41564"
                     /product="hypothetical protein"
     CDS             complement(2130..4112)
                     /locus_tag="BJ085DRAFT_41564"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37765.1"
                     /db_xref="JGIDB:DimcrSC1_41564"
                     /translation="MPAAVAQFGLYISVTRILSSLSVLASLGIVGLGVFLLNWKYRQV
                     NHASFRFAILIAITDVILAVFQLLNTAVLPAPLPTAAGIPSGINPLESSINVLGLQPA
                     FHAFGASESEVLNQSTRGATLFIAWGTHFATLASIFAIIAFIVQSHYELNLVNVHLDA
                     ANKLLRLNKQFERGCLVGSVGLAALFSLVPVILYLAGATSVAGPSASIVLFTDHEITL
                     ASALYWSFLGIWILFGVLFAAYALGWAIQFLLRYQSRQVTSLRQTVTEPSNVLPSPSL
                     CSAANSFDRYRDYSYKFCIRYQARQSVLNGLLSPLSPFPLATVDFVPRRTIFRAVLSV
                     TFYAISLAICYLPFISNDLLAYSGRSSPDLIWSSGVILCCQGLLHALAFFTSPTPYAV
                     LASYRADVYEKQRAREQAIRHGLLLCDDIIASPRSQNLRGQLSPISSITSDHTICCQS
                     PAYLNRKSPIPIFNSSRSLVSLESLQDSLRHHRIKDVSLTRCHSLDTGISASVASQST
                     MSNASIQSLPNSWSASTTRSTPVKVDSLLVRSTNINALPVAPPIGIISTTSARKPSLS
                     NRVMEWFVLRGQQEAPAAPRTIPVLTRHSRPVFDTTIAGRLSSARHNDLALMNGLHDF
                     VETCNVNGFGFGFTVPYRSAWETRSSYYDQTTTIIA"
     gene            complement(<7699..>7950)
                     /locus_tag="BJ085DRAFT_5939"
     mRNA            complement(<7699..>7950)
                     /locus_tag="BJ085DRAFT_5939"
                     /product="hypothetical protein"
     CDS             complement(<7699..>7950)
                     /locus_tag="BJ085DRAFT_5939"
                     /note="domain of unknown function-domain containing
                     protein"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37766.1"
                     /db_xref="InterPro:IPR025187"
                     /db_xref="JGIDB:DimcrSC1_5939"
                     /translation="IQIGLDGLIGLVPAAGDCVNVAIALYLVKIASSTGLPGKYKAKM
                     IRNIAIDGGIGTIPVLGDIFDIIYKANLRNLAIFEKWIGK"
     gene            <10381..>11823
                     /locus_tag="BJ085DRAFT_41566"
     mRNA            <10381..>11823
                     /locus_tag="BJ085DRAFT_41566"
                     /product="hypothetical protein"
     CDS             10381..11823
                     /locus_tag="BJ085DRAFT_41566"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37767.1"
                     /db_xref="JGIDB:DimcrSC1_41566"
                     /translation="MRVRLSLLLLSLINVGSTVLAEPINSDGDTSSTSQNDGKRSLTS
                     TPNITHQDRFSLIMLLAKPHITPDLSYALQTVNRKLYSQHSALAAQCRSNYLCLLRVA
                     VNRGGLVDPSIETILNNLISQVTLLQIFAALDTEIKTWLYSYITPAILGNGGQTGQLN
                     VVSDTVQHALPIPQLPTEIRPVPYLQLNQWADEFPLVNAIKAGPATARFIVTLFEQLV
                     RLVITSRLDQTETTSSLVELIQEGADGAGSSTWDHFRLFNYVQKNTPGFFSDPVILFR
                     RLVMEVFRELFYIAYREKQWDTLQRLLTTIHRHHSVFGRFQTYYHGVLLVAQFAEPGR
                     MAFMEGQLRYMTASDRLTLVTCARHLNLVEAAEFLTDGLCANRSVLSMGLCMLLDPNT
                     SGEGTVHPNLCNGLLDLPERFLVTHRLDDYFQFRVVPDQPYPERLYSEGVDVRHSLPG
                     LTKDSGTSNQRWLAVKKWIKKRASHDQFNQ"
     gene            complement(<12356..>12700)
                     /locus_tag="BJ085DRAFT_33905"
     mRNA            complement(<12356..>12700)
                     /locus_tag="BJ085DRAFT_33905"
                     /product="hypothetical protein"
     CDS             complement(12356..12700)
                     /locus_tag="BJ085DRAFT_33905"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37768.1"
                     /db_xref="JGIDB:DimcrSC1_33905"
                     /translation="MHSIKSAAVLLAMAMAFSYLPSTAASLPYGESSPASQSIHVYRR
                     MFSPNKLDTDLLRSNKGFNGNAQPPLKGNANPKPKIPMGKIPTSKFPSPIANDMKSTV
                     DKIRTAKNSAKK"
     gene            complement(<14346..>15332)
                     /locus_tag="BJ085DRAFT_9201"
     mRNA            complement(<14346..>15332)
                     /locus_tag="BJ085DRAFT_9201"
                     /product="cleft lip and palate transmembrane protein
                     1-domain-containing protein"
     CDS             complement(<14346..>15332)
                     /locus_tag="BJ085DRAFT_9201"
                     /note="GO_component: GO:16021 - integral to membrane"
                     /codon_start=1
                     /product="cleft lip and palate transmembrane protein
                     1-domain-containing protein"
                     /protein_id="RKP37769.1"
                     /db_xref="InterPro:IPR008429"
                     /db_xref="JGIDB:DimcrSC1_9201"
                     /translation="YLPLLGPNSMGTRKEHLKELEPTDQPTWDMPLTVQLKTVALGWY
                     RVTRIMDATFAQLSAGGSPLAVSGNELESLRKMIFEVNPTLLLITIVAACLHMLFETL
                     AVKSDIDHWSRKKSITGISRSSVFMGALTSWLTLLYVWDRRKDSGMLVLTGTAVSAVV
                     ELWKVFKVRRFLRQPAVANEDVSKVSNEKKMNDPVSVDQAIPTTDTASLEDFDNRVVK
                     YLLYLCIPLIIGYAGYSLVFWKHKGFYSWGLDALMASIYLLGFVNMTPQLFLNHRLQS
                     VEAMSLATFIYRAINTFVDDLFALVIPMPGLTRVSAFRDDIIFVVLLYQWWKF"
     gene            <16578..>17200
                     /locus_tag="BJ085DRAFT_24938"
     mRNA            join(<16578..16684,16900..>17200)
                     /locus_tag="BJ085DRAFT_24938"
                     /product="protein-tyrosine phosphatase"
     CDS             join(16578..16684,16900..>17200)
                     /locus_tag="BJ085DRAFT_24938"
                     /codon_start=1
                     /product="protein-tyrosine phosphatase"
                     /protein_id="RKP37770.1"
                     /db_xref="InterPro:IPR004861"
                     /db_xref="JGIDB:DimcrSC1_24938"
                     /translation="MAHLPLIIPPFRFACVEPGVYRGCYPNRLNERFLLRLQLRTIIS
                     LAPNDPNSWLPDYCTQHQIARFSFPTDVPKENVTLQTESIQRCLELLLDPRRHPLYLH
                     CTDGTANTSLIIIALRRLQLWPLTAAIAEYLRFS"
     gene            complement(<19027..>25170)
                     /locus_tag="BJ085DRAFT_9770"
     mRNA            complement(join(<19027..19634,19683..20342,20586..21387,
                     21631..22196,22275..22930,22982..23291,23352..23792,
                     23949..24144,24241..>25170))
                     /locus_tag="BJ085DRAFT_9770"
                     /product="hypothetical protein"
     CDS             complement(join(<19027..19634,19683..20342,20586..21387,
                     21631..22196,22275..22930,22982..23291,23352..23792,
                     23949..24144,24241..>25170))
                     /locus_tag="BJ085DRAFT_9770"
                     /note="GO_function: GO:5086 - ARF guanyl-nucleotide
                     exchange factor activity;
                     GO_process: GO:32012 - regulation of ARF protein signal
                     transduction"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="RKP37771.1"
                     /db_xref="InterPro:IPR000904"
                     /db_xref="InterPro:IPR015403"
                     /db_xref="JGIDB:DimcrSC1_9770"
                     /translation="LEKILKSRDGKRDKKLREALTRALEHLRQNQNSQTGPADTTTPS
                     SAGGHSEAVDATELILNPLRLACATESVPAIIIALDCIEKLISYSYFENPEGGNSAPG
                     SPVSILDQLVDIVCDCFAGEITDDMVQLQVVKAILAAVSCSSNDPAHQLHQSALLKAV
                     RTIYNIYLLSRSESNQTLAQGTLAQIVRIVFGRIKLDTAVIESGESADPASGTAQPSP
                     LSSPAVLKGPAKSPPASTTNPHFARNGESTPVEAALPSDKHQDSTTVNGLQEGAVESP
                     VEDTAPRTGPANSDEPHPAELLAPVAMSVTDSDNAIRDAFSLFRALCKLSMKNMTKEQ
                     YNDIRILSLRSRLLALHLIYIILNTHLDVLTRANVTLRTGNDASEYVTIPFIGVVKQY
                     LSLSLSRNLLSLDFGVFEVSLAILARLLVSLRKFMRKEIEVLFREIILPTIEMRHSAY
                     LQRSCLIQCLLQICNDPQTLIEVYLNYDCSQDSALNLYERLVNTLSKIAAGGSGSGAT
                     NTNSGAAGSNAGTGRELHAGPTIPPNLTTSSVTQALLTHKEYLVPDDYSLKCQSIEAI
                     TGILRSLVSWINRGSADTSGTHPSATDADSLSARANAKSPQPSLLLDSATDAVSSGTS
                     NPLHGFNMATTGTTDDPGQFMYVKQRKQQLEDGLRLFNWKPKKGIQALIKTHFIESDT
                     PEAIAKFLRSADGLNKTQLGEYLGEGDTKNIAIMHAFVDDMAFVNTPFVPALRNFLQD
                     FRLPGESQKIDRFMLKFAERYVEGNPDAFANADTAYVLAYSVILLNTDQHSPQVKVRM
                     TKADFVKNNRGINDNADLPEEFLHAIYDEIQKHEIILKDEHDPMLTDGGVFDDGSSSL
                     GLSSAFGVLRRRSTRYEYYSSMSREMANKSEALIKDILRKRSRLDIANEANLFYQASH
                     IEHVRPMFEIVWMPILAGISGPAQELDDPDIIQLCLESFRHAIHIAATFDLALERDAF
                     VSTLAKFTVLANLMEMKPKNIEAIKTLLDIAAIDGNDLNQSWLDVLTCVSQLERLQLI
                     NDGRSSVITAGKQRPGVTRQHRSLQLADLSRLDANSQSLVVAVDRIFSSSVKLSGTGI
                     VEFVRALSIVSWEEIQNSTFGGHTHPRLYSLQKIVEISYYNMSRIRMEWSNVWAILGD
                     HFNQVGCHPNVQVGFFALDSLRQLSMKFLEKEELAHFKFQKDFLRPFHFILENSPDVS
                     IKDMVLRCLQHIIRSRSDHVRSGWKTILSVLSTAAREPSEPIVVMAFEILREVCKERF
                     NDIVTLGSFPDLILCLTEFCTSSMHQRSSLQAIEMLYLSDALPPNEDPVYRLWFPVFY
                     AMYDIIMNCDDLEVRTKALNYLFEFLKNYGSTFSFEFWSLILRDVVYPIFRDLKSPET
                     SQRFGRQEDITFWFSTTLIKALRNLIDLFTHYYETLCPTLDGILELLSICIAQDSETL
                     ARIGTACFQELIENNHQKFDETAWHQICRTFLNLFNTSVPHVLFEMSGRPVPTSGLNG
                     TGSNFRPVGSNSNNGGGNGHELGGGSDPQEEYQRITLKCVLQLLLIQTVDDLFHKNAS
                     IYRSLESNHLFFILDCLDKSYQFARKFNADVQLRLALVESGFMTQVPSLLKQEVNSLQ
                     CAMSILYRMYADTSSERQLIAVEVEDRLVPLSQNVFIHFNRIDPHTAERHIQAWQPVV
                     VNILENILDMDKSQIHRQMRVLYPEFIRLLAFPRPQHLVGLIQQLLLRIGDIYQI"
     gene            complement(<26962..>29766)
                     /locus_tag="BJ085DRAFT_24928"
     mRNA            complement(<26962..>29766)
                     /locus_tag="BJ085DRAFT_24928"
                     /product="P-loop containing nucleoside triphosphate
                     hydrolase protein"
     CDS             complement(26962..29766)
                     /locus_tag="BJ085DRAFT_24928"
                     /note="GO_function: GO:3676; ATP binding - nucleic acid
                     binding [Evidence 4386] [GO Ref 5524; helicase activity]"
                     /codon_start=1
                     /product="P-loop containing nucleoside triphosphate
                     hydrolase protein"
                     /protein_id="RKP37772.1"
                     /db_xref="InterPro:IPR001650"
                     /db_xref="InterPro:IPR007502"
                     /db_xref="InterPro:IPR011545"
                     /db_xref="InterPro:IPR011709"
                     /db_xref="JGIDB:DimcrSC1_24928"
                     /translation="MGTRDEKSPRDKGRRKDVVPCHLVNDQGDDALDAEERDAFAQRL
                     RVRDRDREHDRSRRSSLGPSSSRPEGSGSSSRTSRSIHHDSHQRQEVTSDLRERSRQE
                     YLKKREQSRLELLEAQIADEEMLFHKSALTDTERRQLEYQKEVLRIAKERKDINVDVE
                     GYVMPEDYLTTKGKIDRKRKEQALYQRYQEPKGSVSGSGKDEKGIGGNLSELEQWEAR
                     QIARTKVKVGPQDKGTIPDADAYDFIIDETGIDFVLESTLDPATEADREAQSLLDAAE
                     RKQKSIQAVRESLPVYDYRDALLAAIEDHPVLIIEGETGSGKTTQIPQYLYEAGYSKD
                     GRKIGCTQPRRVAAMSVAARVAEEMRVHLGREVGYSIRFEDKTSEKTVVKYMTDGMLL
                     REFLTEPDLDGYSCIMIDEAHERTLSTDILFALIKDITRYRPDLRVLIASATMDAQKF
                     SSYFDDAPIFKIPGRPFPVDIYYTLAPEANYLQAAVTTVLQIHASQPTGDILVFLTGQ
                     GEIEQAQELLRQARVALGRRIKDLLVCPIYANLPSDLQAQIFNPTPENARKVVLATNI
                     AETSITIDGIVYVIDPGFQKLKTFDPHTEMESLVVTPCSRNAANQRAGRAGRVGPGKC
                     FRLYTLWAYENELEDHTIPEIQRSNLCMVALQLMSLGINNLVEFDYIDPPSVTTVQKA
                     IGQLYMLGALNAKGEVTKTGRRMAELPLDPKMAKALLASAKYHCSDEIATLCALLDVQ
                     SALYYRPKAEKLNAEKAHQNLVQPGGDHFTLLHIWEQWVQLEYSESWCRENYLQRRSL
                     LRATDIREQIVRLLERIEVPLESNPNPADTSGVAKAMLAGYFSNVAQRQRGEDSYKLA
                     KRKETVLVHPSSSLREAKPPWVMYYELVMTSEKFVRVVMEINPEWLIEVAPHEFKPSD
                     IVDKSKQKMPKFLKKARELI"
CONTIG      join(QRFA01000248.1:1..30912)
//