LOCUS MH823215 18524 bp DNA linear BCT 02-OCT-2018 DEFINITION Staphylococcus aureus strain ABD2005 pathogenicity island genomic sequence. ACCESSION MH823215 VERSION MH823215.1 KEYWORDS . SOURCE Staphylococcus aureus ORGANISM Staphylococcus aureus Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus. REFERENCE 1 (bases 1 to 18524) AUTHORS Ram,G., Ross,H.F., Novick,R.P., Rodriguez-Pagan,I. and Jiang,D. TITLE Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice JOURNAL Nat. Biotechnol. (2018) In press PUBMED 30247487 REMARK Publication Status: Available-Online prior to print REFERENCE 2 (bases 1 to 18524) AUTHORS Ram,G., Ross,H.F., Novick,R.P., Rodriguez-Pagan,I. and Jiang,D. TITLE Direct Submission JOURNAL Submitted (31-AUG-2018) Department of Microbiology and Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA FEATURES Location/Qualifiers source 1..18524 /organism="Staphylococcus aureus" /mol_type="genomic DNA" /strain="ABD2005" /db_xref="taxon:1280" misc_feature 1..18524 /note="pathogenicity island" repeat_region 1..22 /note="target site duplication" /rpt_type=direct gene complement(87..1259) /gene="int" CDS complement(87..1259) /gene="int" /codon_start=1 /transl_table=11 /product="integrase" /protein_id="AYD60529.1" /translation="MASYEKRGNTWRYRISLGKDAETGKYKYISNSGFKRKSDAKHHA EMVERQLRNGDYIAPSTSTFKQVADDWISQYANEVKVSSVRAREKAINHAIERFNNKP IQTINKHEYQRFVNDISAQYSKNYVDSIIASTNMIFKYAYDMKLIRIMPSEGIKRPKK KISVEELEDTEIHKKFLEKDELFQFLEVAKNHHSPQNSFEVFCTLAYTGMRAGELLAL KWSDIDFENNTINITKTYYNPNNNKKQFQILTPKTESSIGKISVDPHVIKLLKNYKTD VQDTWKNELYVDNNFVFTDVNGYPLVIKKLQLWIKAILKKTDITNKQISTHSFRHTHC ALLIEAGVHIKEIQERLRHKDINTTMNIYAKITNSYKKDASQKFSKLMENVSKDLF" gene complement(1273..1947) /gene="stl" CDS complement(1273..1947) /gene="stl" /codon_start=1 /transl_table=11 /product="master repressor" /protein_id="AYD60531.1" /translation="MIRNRLSELLSERGLKISRVAKDVKIARSSLTSMAQNDSEMIRY DAIDKLCSYLHISPSEFFEHNPINFDFTFDEEPNYKINDVFEGFEVTANITHAFSIEN FDFEILVDVELDNRQKLNFDLDVSYKETEKITNSQHRFIFTIKNEDENIGLKKYVDSL SAGLKNLLFKKINQKLSGYVSEIIVKNIDDIEELFPNKGEKSTTLHKEILQTDSRLSS DIFKEY" gene 2120..2338 /gene="str" CDS 2120..2338 /gene="str" /codon_start=1 /transl_table=11 /product="regulatory protein" /protein_id="AYD60530.1" /translation="MTILANTRKFKEAMFLKGFNLSDLSRETGVGISYLSQIINGKKI PSPKLAKKMAEVLQVEVNELFEFEVKEA" gene 2343..2660 /gene="xis" CDS 2343..2660 /gene="xis" /codon_start=1 /transl_table=11 /product="exisionase" /protein_id="AYD60532.1" /translation="MFNINIDEDEARELLEQAINARVDELAKEKYFMTYKELSNYLNL SKPTIEELLINNGMKYYMVGSTYRFKKSDVDEFMEQLTAHMNIQNNDFKQVNIKKLLE ARQ" gene 2657..2812 /gene="orf5" CDS 2657..2812 /gene="orf5" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AYD60533.1" /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYAWLITLGCNLTGE ITTCENK" gene 3002..3385 /gene="orf6" CDS 3002..3385 /gene="orf6" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AYD60534.1" /translation="MGIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD RKGHVIGMLEWALDYIAGNIEVEEL" gene 3386..3703 /gene="orf7" CDS 3386..3703 /gene="orf7" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AYD60535.1" /translation="MNWEIKDLMCDIEAVKEKINDVAIKHAWFVEDRFVKNELETKRE HINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKFHEIEKASSENFGEVSDDAQKLK ITE" gene 3767..4636 /gene="pri" CDS 3767..4636 /gene="pri" /codon_start=1 /transl_table=11 /product="primase" /protein_id="AYD60536.1" /translation="MNEIKLEYDTHVSVVHYESLDSRSFNSFSKINWSKLVNKLSVPI EANYKYARGVAVYGDIKNGANDQGEIIKKHRNDKNVIYRDVIVLDYDEINDLKQLHEA ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYTKVLANKIGHKVDEG SYQPSRCFALPVIQKGHIFIKRVNDCPIMNVDMLEQWSKEFEQSNASPNVIGYTRRDS EYWRELCFGTTEGNRNNALASLIGHLLRCHVNDYIVYSFALLWGQFACKPPMKEQEIN ATFQSILNKHYNN" gene 4650..6359 /gene="rep" CDS 4650..6359 /gene="rep" /codon_start=1 /transl_table=11 /product="hlicase rep" /protein_id="AYD60537.1" /translation="METGKSDVLDKIEKINKKDSALQEIIPKGYEIEHHQCGIALNQL IPSKKEGEPDKKVFITSTIPQITERFEDIESNEVSFNMLFYDNKTPVNIAVSAEEISD SRQLLKLVNKKLDVTSSTSTKLVDYINASKRYNPPLNVKVATRLGHVKGYFIYPYQEV MKDSNIKLFSNDKGFQKLIDSFQSKGTLEGYSKKVFGQIKDLPMVMVMLYASLGSVLL REFGLQPFIVEISGSTSTGKTFTLNLVSSVWGTSDLITTWSSTQNSIESMASFLNSFP MFKDDTRNTHPKFVTSATYNFSSGESKSRSNINLTLNAKKEWRNILISTGESSIANMA DEKAGVSARVVTLQDPPYPDNFDFTTLDKSFRENYGTLGLAFIKQYESKKDVYKNAFE SYQRYFNQKGSNEIMQRLGRAFALLQVTGEVLNDIDGFEHDHFKIIEQAYDSMVKNNK TIDKPKQLLEELLQYLDANRNNIAGDGYSSVKNGDIKAIYKRDYLCILGQTVHDKLGH EMQTITGQWGKKGYLIKGEKDRLQKKVSHKNIKYRGFAINKEMLEELGFDFSNSHNPY SDY" regulatory 6360..6639 /regulatory_class="replication_regulatory_region" gene 6671..7051 /gene="ppi" CDS 6671..7051 /gene="ppi" /codon_start=1 /transl_table=11 /product="phage packaging inhibition" /protein_id="AYD60538.1" /translation="MDKEQLKKYIYDYVKEYKEIPIYQLEDLFKEMNHDYIGRTSVTH DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFVMRYLLDGRVPNLPLAICYPE DGQQTDVPSWVPMVLRINKEEKIK" gene 7048..7689 /gene="orf11" CDS 7048..7689 /gene="orf11" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AYD60539.1" /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM AYVMMNGKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKQLSISD MMASQLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY QLATGINEPAPELVEGLRLVTEFVQDENATQEDYKALEHKLTELKSSYYSLNK" gene 7919..8044 /gene="orf12" CDS 7919..8044 /gene="orf12" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="AYD60540.1" /translation="MARFVQGVRTLLTLIQAKVCVFGIGLKVKFVRCLFVLFYRT" gene 8320..8847 /gene="ptiM" CDS 8320..8847 /gene="ptiM" /codon_start=1 /transl_table=11 /product="phage transcription inhibitor M" /protein_id="AYD60541.1" /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNMALLKKVL NHYTEKEQKQVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI ARVNAYHQSSYVKVV" gene 8850..9191 /gene="ptiA" CDS 8850..9191 /gene="ptiA" /codon_start=1 /transl_table=11 /product="phage packaging inhibition A" /protein_id="AYD60542.1" /translation="MDKKQIKDFVCDYHKRTRSDVLIDDEINTDEFFSIGDENSNEWM ADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSFKDISNVLECSEQSVRLWYE TLLDKIVEVIE" gene 9188..9757 /gene="terS" CDS 9188..9757 /gene="terS" /codon_start=1 /transl_table=11 /product="SaPI terminase" /protein_id="AYD60543.1" /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGD DEDLDKTVQEVSNANPNHTVIVDDIPLED" gene 10856..12790 /gene="tetM" CDS 10856..12790 /gene="tetM" /codon_start=1 /transl_table=11 /product="tetracycline resistance" /protein_id="AYD60544.1" /translation="MEENHMKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKG TTRTDNTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAIL LISAKDGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIK QKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSL FPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLY SGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGD TKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEII LSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASI GLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKY GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKY CANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVC QTRRLNSRIDKVRYMFNKIT" repeat_region complement(13381..13416) /rpt_type=direct repeat_region complement(13447..13482) /rpt_type=direct gene complement(13635..17741) /gene="dcas9" CDS complement(13635..17741) /gene="dcas9" /codon_start=1 /transl_table=11 /product="dCas9" /protein_id="AYD60545.1" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD" BASE COUNT 6318 a 2832 c 3244 g 6130 t ORIGIN 1 atgccaggaa tgatgtaaaa tgactgttaa acgccgattt tataacgttt gtaatattgg 61 gtggtcataa tttggtcata gaaattttaa aataaatctt ttgagacgtt ttccatgagt 121 ttactaaact tttgagaagc gtcttttttg tatgagttcg taatcttagc gtagatgttc 181 atagtggtat ttatatcttt atggcgcaag cgttcttgta tttccttaat atgcacacca 241 gcctctataa gtaacgcaca atgagtatga cgaaatgaat gagtgcttat ttgtttatta 301 gttatgtcag tctttttaag tatagctttt atccataatt gtagtttttt aattacaaga 361 gggtagccgt taacatcagt aaaaacgaaa ttattatcta catacaattc gtttttccat 421 gtgtcctgca cgtcggtttt ataattttta agtaatttaa tcacatgagg atccactgaa 481 atttttccga ttgagctttc agtttttggt gtaagtattt gaaattgctt tttattgtta 541 ttcggattgt aataagtctt tgtaatattg attgtgttat tctcaaagtc tatatcagac 601 catttcaatg ccaataattc acctgcacgc atgcctgtat atgctaatgt acaaaacacc 661 tcaaagctgt tttggggtga atggtgattt ttagcaacct ccaggaattg aaataattca 721 tctttttcaa gaaacttttt atgtatctca gtatcttcta attcttccac actaattttc 781 tttttaggtc gtttaatacc ctcgctaggc attattctta ttaatttcat atcgtatgcg 841 tacttaaata tcatatttgt agaggctata atgctatcaa cataattctt gctatactgt 901 gcgcttatat cgtttacaaa acgttgatat tcatgtttat tgatagtttg tattggttta 961 ttgttaaagc gttctatggc gtggtttatg gctttctcgc gtgctctgac actacttact 1021 tttacttcgt tagcatattg tgatatccaa tcgtcagcaa cctgtttaaa tgtagatgtg 1081 gacggtgcga tataatcgcc atttcttaac tgacgctcaa ccatttcagc gtgatgttta 1141 gcgtctgatt tgcgtttaaa acctgagttt gaaatatatt tatatttgcc cgtttctgcg 1201 tcttttccta gtgatatacg atagcgccat gtatttccgc gtttttcata acttgccatt 1261 tgatcacctc gattaatatt ctttaaaaat atcactagat aaacggctat cagtttgtaa 1321 aatttcttta tgtagagtcg tacttttttc gcctttattt ggaaaaagct cttcaatatc 1381 gtctatattt tttactatta tttcagaaac atacccactt aacttttggt taattttttt 1441 aaataacaag tttttaaggc ctgcagataa actatcaacg tattttttta atccgatatt 1501 ttcatcttca tttttaatcg tgaaaataaa tctatgttgt gaattagtta tcttttcagt 1561 ttctttatat gagacgtcta agtcaaaatt taatttttgc ctattatcta attcgacgtc 1621 tactaaaatt tcaaagtcaa aattttcaat cgaaaaagcg tgagtaatgt ttgcagttac 1681 ttcaaatccc tcgaaaacat cattaatttt ataattcggt tcttcatcaa aagtaaagtc 1741 aaaattgatc ggattatgtt caaaaaattc tgaaggagat atgtgcagat aactacataa 1801 tttatctata gcatcatatc ttatcatttc agaatcattt tgtgccattg aagtaagtga 1861 acttcttgct atttttacat cttttgcaac acgagatatt tttagtcctc tttctgacag 1921 tagttcagac aatctatttc taatcattac aaacctccta attatgttaa taatagcatt 1981 tttttggacg tttatgtaca aaaaaataaa aaatgattga gaagtcagtc gaaaaactat 2041 tgcaaaagaa aaacgattat gtataataaa gttataaatt gattgagaag tcagtcaaaa 2101 acgaaggagg attttaatta tgactatttt agcgaatact agaaagttta aagaagccat 2161 gttcttaaaa ggctttaatt tatctgattt atcacgtgaa acaggtgttg gaatttctta 2221 tttaagccaa attattaatg gtaaaaagat tccaagccct aaattagcta agaaaatggc 2281 agaagtttta caagttgagg taaatgaatt atttgaattt gaagtaaagg aggcataaac 2341 caatgttcaa cattaatatt gatgaagatg aagcacgtga gttacttgag caggctatca 2401 atgcacgtgt ggacgaatta gcgaaagaga aatattttat gacttacaaa gagttgtcta 2461 actatctgaa tttaagtaag cctactattg aagaattact tattaataat ggcatgaaat 2521 attatatggt cggatctacg tacagattca aaaagtctga tgtagatgaa ttcatggaac 2581 agcttactgc tcatatgaat atccagaata acgactttaa acaagtcaat atcaaaaagt 2641 tattggaggc aaggcaatga aaatctactt aacttatatc tgcttagttt cattgttaac 2701 aatattatta ctagcaatat ctaacatgta tgttgctttt agcgtttatg cttggctaat 2761 aactttagga tgtaatttaa caggagagat tacaacgtgc gaaaacaagt gattattaca 2821 aaaacagtag ttggctggta caacattaaa gatactcaac ataatttaat gttaaatata 2881 ccgccaaaag tatttgaaca gtactttcct gatgttagta aagatgttca agttgtgtgt 2941 ttagaaatgg atttatcaaa aattacagaa attaaaaata agaaaaaagt aggtagttaa 3001 gatgggaatc aaacaaaaat atcaattatc aaaagtggtt aaaatattag aagtagtatt 3061 atacgaggaa gataagtttc aatccgataa ggactatcat tatcaggata aagcattata 3121 tgaatatgct ttaaagttag ttcataatgg attgttcaat attcttgctg aattagattt 3181 tgaagatgaa gcatttttaa ttcttgatga agtaacaatg acgctaagtg atgtcatgaa 3241 agaaacacaa cacgtttacc gttatagtgt catagatgaa aaaggtgaac acaaacatac 3301 aacagatcgc aaaggacacg tgattggaat gttagagtgg gcattagatt acattgcggg 3361 aaatattgaa gtggaggaat tataaatgaa ttgggaaatt aaagatttaa tgtgtgatat 3421 tgaagcggta aaagaaaaaa tcaatgatgt agctatcaaa catgcttggt ttgttgaaga 3481 tagatttgta aaaaatgaat tagaaacaaa acgggaacat attaattttt ctgctagcta 3541 tttagaacat cgtatacaaa atgaacatac agttgagtta ttacatgtgt acttaaaaga 3601 attcggtgaa cttatacaaa aatttcatga aatagaaaaa gcatcatctg agaactttgg 3661 cgaggtatca gatgacgcac aaaaattaaa aatcacagag taatttagaa attacacatg 3721 tttattataa cattttttac tctgtgaatc actagaggtg caaaaaatga atgaaattaa 3781 attagaatat gacacacatg tttcagtggt acattatgaa agtttagact cacgttcatt 3841 taatagcttt tcaaaaatta attggagtaa gttggttaat aaactgtctg tacctataga 3901 agcaaattat aagtatgcac gtggtgttgc tgtttacggt gatattaaaa acggtgcaaa 3961 tgatcaaggt gaaattatca aaaagcatcg aaacgataaa aatgtcatat acagagatgt 4021 gattgtactt gattatgatg aaataaatga tttaaagcaa ttacatgaag caatcagctc 4081 agctttaagc aatgttgcat ggttttggca cacaagttac tcgcacagaa ctgaacaagc 4141 tagaatacgc ctgtatatcc ctctaaatga gcgaataagt gcagatgatt atcgtaaata 4201 tacaaaagta ttagcaaata aaattggcca taaagtggat gaaggttcat atcagccaag 4261 tagatgtttt gcgttaccag ttattcaaaa aggacacata tttattaagc gagtgaatga 4321 ctgtccaatt atgaatgttg atatgctcga acagtggtcg aaggagtttg aacaatcaaa 4381 tgctagtcct aatgtcatag gatacactcg acgcgatagt gagtactggc gcgagctatg 4441 ctttggaaca accgaaggca atcgtaacaa tgcactagct agcttaattg ggcatttatt 4501 aagatgtcac gttaatgatt atattgttta ttcatttgct ttactatggg ggcaattcgc 4561 atgtaaacca cctatgaaag aacaagaaat caacgccact tttcaatcga tattaaataa 4621 acactataac aattagaaag gggctttgta tggaaacagg taaaagtgat gtacttgata 4681 aaattgaaaa aattaataaa aaagatagtg ccttacaaga aattatacca aaaggttatg 4741 aaattgaaca tcatcaatgc ggtattgcct taaatcaact tataccaagt aaaaaagaag 4801 gcgagccaga taaaaaggtt tttatcacaa gtacaatccc tcaaatcact gaacgctttg 4861 aagatattga gagtaacgaa gtcagcttta atatgctttt ctatgacaat aaaacgccag 4921 taaatatagc tgtgagtgcc gaagaaattt cagatagtcg tcaactcttg aaattggtta 4981 ataaaaagct ggatgtaaca tcgtcgacat ctactaaact tgttgattat attaatgcat 5041 ctaaacggta taatccacca ttgaatgtta aagttgcaac gcgtttgggg catgtgaaag 5101 gttattttat ttatccttat caagaagtga tgaaagacag caatatcaag ttgtttagta 5161 atgataaagg atttcaaaag ttaatagact cttttcaaag caaaggaaca ttagaaggtt 5221 actctaaaaa agtgttcggt caaataaaag atctaccaat ggtaatggtt atgttatatg 5281 cctctttagg ttcggtttta ttaagagaat ttggattaca gccctttatt gtagaaatat 5341 caggtagtac atctacaggt aaaacattca cactcaactt agtatcaagt gtttggggaa 5401 cgagcgacct tatcacgaca tggagttcta ctcaaaatag tattgaatcg atggcatcat 5461 ttttgaactc atttccaatg tttaaagatg atacacgtaa tacacatcct aagtttgtta 5521 ccagtgccac atataacttt tctagtggtg aaagtaaatc aagaagtaat attaatttaa 5581 cactaaacgc taaaaaagaa tggcgaaata ttttaatttc tactggtgaa tcatctatcg 5641 caaatatggc tgatgaaaaa gcgggtgtat cagcacgtgt agttacacta caagatccac 5701 catatccaga taattttgat tttaccacat tagacaaatc gtttagggag aactatggaa 5761 cgttagggtt ggcatttatt aaacaatatg agtctaaaaa agacgtgtat aagaacgctt 5821 ttgagagcta tcaacggtat tttaatcaaa aaggtagtaa tgaaatcatg caacgtttag 5881 gacgtgcctt tgcgttacta caagttaccg gtgaggtttt gaatgatatt gatgggtttg 5941 aacatgacca ttttaaaatt atcgaacaag cctatgacag catggttaaa aacaataaga 6001 cgattgataa acctaagcaa ctgttagagg aactattaca atatttagat gcaaatagaa 6061 ataatatcgc tggtgatggc tatagttcag tcaaaaatgg tgacatcaaa gctatatata 6121 aacgtgatta tttatgtata ttaggtcaaa ctgtacacga taaattaggt catgaaatgc 6181 agactataac aggtcaatgg ggcaaaaaag gatatttaat taaaggtgaa aaagatcgct 6241 tgcaaaaaaa ggtgagtcac aaaaacatta agtatagagg atttgctata aacaaagaaa 6301 tgcttgaaga attaggattt gatttctcga attctcataa tccttattca gattattaaa 6361 tagttcccaa agttcccgat aagttcccgc gaaaaacata caaacgggaa ctataagact 6421 actttaacca caagcaatta aagttaatag ttcccgaagt tcccaataaa taatattatt 6481 atttattatt tgaaaacgaa caaatgttgt tagctttata ccatatatga tagaaaattt 6541 ttaacgggta caacgggaac taagtttatt taaagtttat atatcaatgg tttgactagt 6601 tcccgataag tattttaagt cgggaattca acggggacta gttcccattt aaaaatattg 6661 gaggtaacac atggataaag agcaacttaa aaagtatata tacgattatg taaaagaata 6721 taaggagata ccgatatatc agttagaaga tttgtttaaa gaaatgaatc acgactatat 6781 agggagaacc agtgtcacac acgataagga tgagaatatt gtgttttgga gtggatggaa 6841 caaaattaca atgtttgcgc tgattgaatt agttaaaagt gaacaacttg atttagtgta 6901 tagaggtagt tttgtaatgc gttatttgtt ggatggtaga gttcctaact taccattagc 6961 aatttgttat ccagaagatg gacaacaaac ggacgtgccc tcatgggtgc ctatggtatt 7021 aagaataaat aaagaggaga aaatcaaatg aacatagaaa ctatcgtaaa ccaatttgaa 7081 acacgagcag gcacgttact aaggtactac acaggattat tagaacatag taaagtgcaa 7141 ccatgttgct ttaagttata caatgatcca tttgatatgg catacgtgat gatgaatggg 7201 aagttattcg gtcatgtata tattaaagat tgtaaagtaa ggcaatcatt tgaattagcg 7261 tcacctaagc acactgaggg gcttataaga agtatagaag gtcattatgt aggttatgaa 7321 ttacatgacg gtaaacagct ttctattagt gatatgatgg ccagtcaatt atttgaagat 7381 gagtatttta tgtatggatt acaaacatat gcagaatcaa ataatagtga tgtgtttgag 7441 tacctagaaa atggatttga taccgataca cttgagggca ttcaatcgag taatactgat 7501 gtgatagcga atattgaaat gttgtatcag ttagctacag gaatcaatga accagcacca 7561 gagttagttg aggggttgag attagtaact gagtttgtac aagatgagaa tgcgacacaa 7621 gaggattaca aggctttaga gcataagtta actgagttga agtcatctta ttacagtttg 7681 aataagtaat taaatatgga gtctcacgtg gtgtgtggct cctaatataa aagtataagg 7741 tatagaagtt ttaaaatgta aaggttgcaa caatagtgag ttaatagata ggtaggcgaa 7801 attcaaaaaa gtgtgaaatg ttgatattga gctgttttat ggctttgaaa ataataaggt 7861 tatataaagg tgttagcttt taacatcgga aggtatacag tctttgagaa ttgaaaaaat 7921 ggcaagattt gtgcaaggtg tgagaacttt gttaacgcta atacaagcta aagtttgtgt 7981 ttttggcata ggcctaaaag ttaagtttgt tcgctgtttg ttcgtgttat tttatcgaac 8041 ttaagttcta tattaggtta atgtgaaaag cctaacgtta agtttataac atgattttat 8101 aagtgttata tatgataagc taaacaattg ataaaacgcg ctataaagcg aacgtaagtt 8161 tgttttagac ctgtaaaaat ggtataattt aggtatgaaa taattaaaag aaagaggtgt 8221 agaaatgcaa agtatcaatt tagctaagca aaacaggatt attaaatagc aatgattgcc 8281 tatccaattc gggtaggctc tgtttatagg ggtgaataaa tgaaactgct taaaacgaag 8341 aattgtttat attatcgtaa tggcgacaat aaactatctg agtatcaact attaacgcaa 8401 tttaacccag catttattaa taaaaaaatt aagatgtgtg aattccaaat tgaaagtatg 8461 taccatatga gtgcgtcgac cacaacatgt gatgaaataa tgggggtcgt gtctgtctca 8521 tatccgattg aaaaattagt tatcaaaatt attgaaacaa aagcagggtt acaaaactat 8581 aaaaatagat ctataaataa tatggcgttg ttgaaaaagg tactaaatca ttatacagaa 8641 aaagagcaga agcaagttgt aaaatatatg cgttcaaatg gacgatataa gccttacaac 8701 gtcattgaac gcttacaagt tgatttgtat caagcaagta ttaaacaacg ttcagaacgt 8761 caaaaacaaa gaaatacagc aattgaaaac agtaagattg cacgagtaaa tgcatatcac 8821 caatcttcat atgtaaaagt ggtgtaacaa tggataaaaa gcaaataaaa gacttcgttt 8881 gtgattatca taagcgaact agaagtgatg tgttgataga tgatgaaata aataccgatg 8941 aattcttttc aataggtgat gaaaattcta atgaatggat ggcagacgat aacattgatg 9001 atcatattgt aaagaatcac ttagaaatga ttgttgacca agtagctaat gacaaagagt 9061 tttatatttt cgattcttta atacaaggac gtagttttaa agatattagc aatgtcttag 9121 agtgttcaga acaatctgta agattatggt atgaaacctt attagataaa attgtggagg 9181 tgatagaatg agtgagttaa cggcaaaaca agcgcgtttt gtgaatgagt atataagaac 9241 acttaatgta acacaaagtg ccataaaagc aggctatagc gcaaatagtg cacatgtgac 9301 aggatgtagg ttattaaaga agccacacat caagcaatat atacaagaac aaaaagataa 9361 gattatagat gagaatgtat taaccgcaaa agagttacta catgtgctta cgaatgcggc 9421 agtcggtgat gagacagaaa cgaaagaagt tgtagtcaag cgaggggaat ataaagagaa 9481 tccacaaagt ggcaaagtac agctagtcta taacgaacat gttgaactga tagaggtacc 9541 aataaaacct agtgatcgtt taaaagctcg tgatatgttg ggtaaatacc ataagttatt 9601 tacagataag catgatatta acgggaatgt gcctatattc attaatattg gtgaatggga 9661 tggcgatgat gaagatttag ataagacggt acaagaggta tctaacgcta atcctaatca 9721 tactgtgatt gtggatgata taccgttaga ggattgatta cagtaaaaac gattatcata 9781 ttgagttagt gaggattagt ttactaattc accctagctt tatattaaag cgttataaag 9841 ataaaaggga gaacgcttat tataattaac ggactccctt tattaataat tattacagaa 9901 aaagtggtaa attaattaat ttctgcttct atagttttta tttcatcaat atttataggt 9961 ggtttttcag tattgtattc aaacttttta gataaatcac tttgatatgt ggatccgtca 10021 ttcattgtta ttttccaata accacccgtt ttatcgcttg aacgatataa tccatgtatt 10081 tgagttagct gatgacgaat ttcaaagtct aaagttgata tagctaattg ttttttatcg 10141 aactttggcc aatactttaa ggggctatct ttaccatgaa ccttaacttt taaaggtagt 10201 tctattggag taggtaattt ttcagtattt gtaacgccac ttatttggaa atggatataa 10261 gttccttcgc tagtatgttg gcttttttta gttctttttg tgtttaagtc aactttttcc 10321 ccttttgtaa aagcagggct ataataagga ctcggaaaaa ttataaggct gatgctgcca 10381 tctgtgtttt ttatacgcat agatcctaag acggatcaag aaacaaaggc aacccaaatc 10441 tcgcaatttg agttgatatt agaaaaacaa gataattgga agattgtaaa ataacaaata 10501 ttggtacatg attacagata ctttgtaatc atgtactctt tttgataaaa aattggagat 10561 tcctttacaa atatgctctt acgtgctatt atttaagtga ctatttaaaa ggagttaata 10621 aatatgcggc aaggtattct taaataaact gtcaatttga tagcgggaac aaataattag 10681 atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt 10741 gattctaaag tatccagaga atatctgtat gctttgtata cctatggtta tgcataaaaa 10801 tcccagtgat aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga 10861 ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac 10921 taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga 10981 caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca 11041 gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg 11101 acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct 11161 actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag 11221 gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt 11281 atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa 11341 ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac 11401 ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc 11461 attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta 11521 tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa 11581 attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga 11641 atatacaaaa aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt 11701 acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc 11761 aataaatggt gaattatgta agattgatag agcttattct ggagaaattg ttattttgca 11821 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa 11881 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca 11941 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata 12001 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga 12061 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac 12121 agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc 12181 gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg 12241 aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt 12301 tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg tgacggattg 12361 taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag cagattttcg 12421 gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag aattgttaga 12481 gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg catataacga 12541 tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg aggtcattat 12601 tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa ctttttttac 12661 aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca ctggcgaacc 12721 tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata tgttcaataa 12781 aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa taagatgaaa 12841 tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca 12901 agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga gtggatacta 12961 tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta attaatgttc 13021 aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc cgataatttg 13081 agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac tgactagcct 13141 tcaatatttc ttgcttcatt gatacctttt gctagtgctt ccattaagga tagttctttg 13201 tctgtaaagc tatccatgta tttttctacc tgtaaccttc gagtgctttt aatcaaatta 13261 tctgtaggta agaaaaattc atcaatagat acatggagta atgatacaag atcccctaag 13321 gaattatcta aaacttcact atttgtactc gaggaactca acaagtctca gtgtgctgaa 13381 gttttgggac cattcaaaac agcatagctc taaaacgaga cctttgagct tccgagactg 13441 gtctcagttt tgggaccatt caaaacagca tagctctaaa acctcgtaga ctatttttgt 13501 ctaaaaaatt tcgtaatcgc actatttgtc tcagctagac ttcagtcttg aaaagcccct 13561 gtattactgc atttattaag agtattatac catattttta gttattaaga aataatcttc 13621 atctaaaata tacttcagtc acctcctagc tgactcaaat caatgcgtgt ttcataaaga 13681 ccagtgatgg attgatggat aagagtggca tctaaaactt cttttgtaga cgtatatcgt 13741 ttacgatcaa ttgttgtatc aaaatattta aaagcagcgg gagctccaag attcgtcaac 13801 gtaaataaat gaataatatt ttctgcttgt tcacgtattg gtttgtctct atgtttgtta 13861 tatgcactaa gaactttatc taaattggca tctgctaaaa taacacgctt agaaaattca 13921 ctgatttgct caataatctc atctaaataa tgcttatgct gctccacaaa caattgtttt 13981 tgttcgttat cttctggact acccttcaac ttttcataat gactagctaa atataaaaaa 14041 ttcacatatt tgcttggcag agccagctca tttccttttt gtaattctcc ggcactagcc 14101 agcatccgtt tacgaccgtt ttctaactca aaaagactat atttaggtag tttaatgatt 14161 aagtcttttt taacttcctt atatccttta gcttctaaaa agtcaatcgg atttttttca 14221 aaggaacttc tttccataat tgtgatccct agtaactctt taacggattt taacttcttc 14281 gatttccctt tttccacctt agcaaccact aggactgaat aagctaccgt tggactatca 14341 aaaccaccat atttttttgg atcccagtct tttttacgag caataagctt gtccgaattt 14401 ctttttggta aaattgactc cttggagaat ccgcctgtct gtacttctgt tttcttgaca 14461 atattgactt ggggcatgga caatactttg cgcactgtgg caaaatctcg ccctttatcc 14521 cagacaattt ctccagtttc cccattagtt tcgattagag ggcgtttgcg aatctctcca 14581 tttgcaagtg taatttctgt tttgaagaag ttcatgatat tagagtaaaa gaaatatttt 14641 gcggttgctt tgcctatttc ttgctcagac ttagcaatca ttttacgaac atcataaact 14701 ttataatcac catagacaaa ctccgattca agttttggat atttcttaat caaagcagtt 14761 ccaacgacgg catttagata cgcatcatgg gcatgatggt aattgttaat ctcacgtact 14821 ttatagaatt ggaaatcttt tcggaagtca gaaactaatt tagattttaa ggtaatcact 14881 ttaacctctc gaataagttt atcattttca tcgtatttag tattcatgcg actatccaaa 14941 atttgtgcca catgcttagt gatttggcga gtttcaacca attggcgttt gataaaacca 15001 gctttatcaa gttcactcaa acctccacgt tcagctttcg ttaaattatc aaacttacgt 15061 tgagtgatta acttggcgtt tagaagttgt ctccaatagt ttttcatctt tttgactact 15121 tcttcacttg gaacgttatc cgatttacca cgatttttat cagaacgcgt taagacctta 15181 ttgtctattg aatcgtcttt aaggaaactt tgtggaacaa tggcatcgac atcataatca 15241 cttaaacgat taatatctaa ttcttggtcc acatacatgt ctcttccatt ttggagataa 15301 tagagataga gcttttcatt ttgcaattga gtattttcaa caggatgctc tttaagaatc 15361 tgacttccta attctttgat accttcttcg attcgtttca tacgctctcg cgaatttttc 15421 tggccctttt gagttgtctg attttcacgt gccatttcaa taacgatatt ttctggctta 15481 tgccgcccca ttactttgac caattcatca acaactttta cagtctgtaa aatacctttt 15541 ttaatagcag ggctaccagc taaatttgca atatgttcat gtaaactatc gccttgtcca 15601 gacacttgtg ctttttgaat gtcttcttta aatgtcaaac tatcatcatg gatcagctgc 15661 ataaaattgc gattggcaaa accatctgat ttcaaaaaat ctaatattgt tttgccagat 15721 tgcttatccc taataccatt aatcaatttt cgagacaaac gtccccaacc agtataacgg 15781 cgacgtttaa gctgtttcat caccttatca tcaaagaggt gagcatatgt tttaagtctt 15841 tcctcaatca tctccctatc ttcaaataag gtcaatgtta aaacaatatc ctctaagata 15901 tcttcatttt cttcattatc caaaaaatct ttatctttaa taatttttag caaatcatgg 15961 taggtaccta atgaagcatt aaatctatct tcaactcctg aaatttcaac actatcaaaa 16021 cattctattt ttttgaaata atcttctttt aattgcttaa cggttacttt tcgatttgtt 16081 ttgaagagta aatcaacaat ggctttcttc tgttcacctg aaagaaatgc tggttttcgc 16141 attccttcag taacatattt gacctttgtc aattcgttat aaaccgtaaa atactcataa 16201 agcaaactat gttttggtag tactttttca tttggaagat ttttatcaaa gtttgtcatg 16261 cgttcaataa atgattgagc tgaagcacct ttatcgacaa cttcttcaaa attccatggg 16321 gtaattgttt cttcagactt ccgagtcatc catgcaaaac gactattgcc acgcgccaat 16381 ggaccaacat aataaggaat tcgaaaagtc aagatttttt caatcttctc acgattgtct 16441 tttaaaaatg gataaaagtc ttcttgtctt ctcaaaatag catgcagctc acccaagtga 16501 atttgatggg gaatagagcc gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga 16561 tttagtttca ccaataattc ctcagtacca tccatttttt ctaaaattgg tttgataaat 16621 ttataaaatt cttcttggct agctccccca tcaatataac ctgcatatcc gttttttgat 16681 tgatcaaaaa agatttcttt atacttttct ggaagttgtt gtcgaactaa agcttttaaa 16741 agagtcaagt cttgatgatg ttcatcgtag cgtttaatca ttgaagctga taggggagcc 16801 ttagttattt cagtatttac tcttaggata tctgaaagta aaatagcatc tgataaattc 16861 ttagctgcca aaaacaaatc agcatattga tctccaattt gcgccaataa attatctaaa 16921 tcatcatcgt aagtatcttt tgaaagctgt aatttagcat cttctgccaa atcaaaattt 16981 gatttaaaat taggggtcaa acccaatgac aaagcaatga gattcccaaa taagccattt 17041 ttcttctcac cggggagctg agcaatgaga ttttctaatc gtcttgattt actcaatcgt 17101 gcagaaagaa tcgctttagc atctactcca cttgcgttaa tagggttttc ttcaaataat 17161 tgattgtagg tttgtaccaa ctggataaat agtttgtcca catcactatt atcaggattt 17221 aaatctccct caatcaaaaa atgaccacga aacttaatca tatgcgctaa ggccaaatag 17281 attaagcgca aatccgcttt atcagtagaa tctaccaatt tttttcgcag atgatagata 17341 gttggatatt tctcatgata agcaacttca tctactatat ttccaaaaat aggatgacgt 17401 tcatgcttct tgtcttcttc caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca 17461 tctactttcg ccatctcatt tgaaaaaatc tcctgtagat aacaaatacg attcttccga 17521 cgtgtatacc ttctacgagc tgtccgtttg agacgagtcg cttccgctgt ctctccactg 17581 tcaaataaaa gagcccctat aagatttttt ttgatactgt ggcggtctgt atttcccaga 17641 accttgaact ttttagacgg aaccttatat tcatcagtga tcaccgccca tccgacgcta 17701 tttgtgccga tagctaagcc tattgagtat ttcttatcca tttttgcctc ctaaaataaa 17761 aagtttaaat taaatccata atgagtttga tgatttcaat aatagtttta atgacctccg 17821 aaattagttt aatatgcttt aatttttctt tttcaaaata tctcttcaaa aaatattacc 17881 caatacttaa taataaatag attataacac aaaattcttt taaaaagtag tttattttgt 17941 tatcattcta tagtattaag tattgtttta tggctgataa atttctttga atttctcctt 18001 gattatttgt tataaaagtt ataaaataat cttgttggaa ccattcaaaa cagcatagca 18061 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt 18121 ttgatacttc tattctactc tgactgcaaa ccaaaaaaac aagcgctttc aaaacgcttg 18181 ttttatcatt tttagggaaa ttaatctctt aatcctttta tcattctaca tttaggcgct 18241 gccatcttgc taaacctact aagctccaca ggatgatttc gtaactgcag ataagcatta 18301 tattgcaatg aaaaagcatt atatggataa tcatatctat cattgcaaat atacttatag 18361 agatttatgt gtgtgataat tggtggtcat aaattggtca taatgaaata aaaaaactaa 18421 aaaaattgaa tgcataaaga atacacgatg ctgatttaat aggatttttg tatatgattt 18481 atatctattt catactgccc ttaatgccag gaatgatgta aaat //