LOCUS       CLOFIXB                 1222 bp    DNA     linear   BCT 06-DEC-2001
DEFINITION  Clostridium saccharobutylicum FIXB (fixB) gene, complete cds.
ACCESSION   M91817
VERSION     M91817.1
KEYWORDS    .
SOURCE      Clostridium saccharobutylicum
  ORGANISM  Clostridium saccharobutylicum
            Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Lin,F.-P., Reid,S.J. and Woods,D.R.
  TITLE     Independent transcription of the linked Clostridium acetobutylicum
            fixB, a-hbd and adh1 genes in C. acetobutylicum P262 and
            Escherichia coli
  JOURNAL   Unpublished
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Clostridium saccharobutylicum"
                     /mol_type="genomic DNA"
                     /strain="P262"
                     /type_material="type strain of Clostridium
                     saccharobutylicum"
                     /db_xref="taxon:169679"
     gene            169..1219
                     /gene="fixB"
     regulatory      169..174
                     /regulatory_class="minus_35_signal"
                     /gene="fixB"
     regulatory      190..195
                     /regulatory_class="minus_10_signal"
                     /gene="fixB"
     regulatory      199..205
                     /regulatory_class="ribosome_binding_site"
                     /gene="fixB"
     CDS             215..1219
                     /gene="fixB"
                     /codon_start=1
                     /transl_table=11
                     /product="FIXB"
                     /protein_id="AAA23237.1"
                     /translation="MNIADYKGVWVFAEQREGELQKVSLELLGEGRRVADKLGVKLTA
                     LLLGSNVEGIKDLAEHGADEVLVADNKDLQHYTTDAYTKVICDLANERKPGILFVGAT
                     FIGRDLGPRVAARLNTGLTADCTSIDVEVENGDLLATRPAFGGNLMATIACPEHRPQM
                     ATVRPGVFEKVNTDGANCKVEKVEVKLTNNDLRTKVLEIIKSKKDIVDISEAKIIVAG
                     GRGVGSKENFELLGELAKVLGGTVAGSRAAVEKGWIENAYQVGQTGKTVKPSIYIACG
                     ISGAIQHVAGMQDSDMIIAINKDETAPIMKVADYGIVGDVKNVLPELIAQAKEIISAE
                     "
BASE COUNT          464 a          150 c          271 g          337 t
ORIGIN      
        1 gatcaaatca aggttatgac tgcagatgat atagatgtag ataaagctga attaggactt
       61 aaaggttcac ctacaaaggt taagaagtca atgactaaag aagttaaggg cgcaggagaa
      121 cttgtaaaag aatcagctaa agaatcagtt agttatgttt tcggaaaatt aagagaaaaa
      181 cactacatct aagataatag gagggtaatt tattatgaat atagcagatt acaaaggcgt
      241 ttgggtcttt gctgaacaaa gagaaggcga attacaaaaa gtatctttag aactacttgg
      301 agaaggtaga agagttgctg ataaattagg agtaaagtta acagctttat tactaggaag
      361 caatgtagaa ggtatcaaag acttagcaga acatggtgca gatgaagttt tggttgcaga
      421 taataaagac ttacaacact atactactga tgcatataca aaagttattt gtgatttagc
      481 aaatgaaaga aagccaggaa tattatttgt tggagctact ttcataggta gagatttagg
      541 tcctagagtt gcagctagat taaatactgg acttacagct gactgtacat caatcgatgt
      601 tgaagttgaa aatggtgatt tattagctac aagaccagca tttggtggta acttaatggc
      661 aacaatcgct tgtccagaac acagaccaca aatggctaca gtaagaccag gagttttcga
      721 aaaagttaac actgatggag ctaactgtaa agtagaaaaa gttgaagtta agttaactaa
      781 taatgatctt agaactaaag ttttagaaat cattaagagc aaaaaggata ttgtagatat
      841 ctcagaagct aagattatag tagcgggggg tagaggagta ggttctaaag aaaacttcga
      901 attactagga gaattagcaa aagttttagg tggaactgtt gcaggttcaa gagctgctgt
      961 tgaaaaagga tggatagaaa atgcttacca agttggtcaa actggtaaaa ctgttaaacc
     1021 atcaatatat atagcttgtg gtatttcagg agctatccaa cacgttgctg gtatgcaaga
     1081 ttctgatatg atcatagcta taaataaaga tgaaactgca ccaataatga aagttgcaga
     1141 ctatggtata gttggagacg ttaagaatgt tttacctgaa ttaattgctc aagctaaaga
     1201 aataataagt gctgaataat tt
//