LOCUS       MEFALADH                1054 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  Methanothermus sociabilis porphobilinogen synthase (aladh) gene,
            complete cds.
ACCESSION   M90083
VERSION     M90083.1
KEYWORDS    5-aminolevulinic acid dehydratase; Zn-binding protein; aladh gene;
            porphobilinogen synthase.
SOURCE      Methanothermus sociabilis
  ORGANISM  Methanothermus sociabilis
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanothermaceae; Methanothermus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Brockl,G., Berchtold,M., Behr,M. and Konig,H.
  TITLE     Sequence of the 5-aminolevulinic acid dehydratase-encoding gene
            from the hyperthermophilic methanogen, Methanothermus sociabilis
  JOURNAL   Gene 119 (1), 151-152 (1992)
   PUBMED   1398086
COMMENT     Original source text: Methanothermus sociabilis (strain KF1-Fl)
            (library: DSM 34966) DNA.
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Methanothermus sociabilis"
                     /mol_type="genomic DNA"
                     /strain="KF1-Fl"
                     /db_xref="taxon:2181"
                     /tissue_lib="DSM 34966"
     gene            19..993
                     /gene="aladh"
     regulatory      19..23
                     /regulatory_class="ribosome_binding_site"
                     /gene="aladh"
     CDS             31..993
                     /gene="aladh"
                     /EC_number="4.2.1.24"
                     /function="condensation of two 5-aminolevulinic acid
                     molecules to porphobilinogen"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="AAA73226.1"
                     /translation="MKFPYTRMRRLRKNSKIRSLVKETTLSKDDLIYPVFVKEGIKTK
                     EKIPKMPGQYRYSVDELIDEAKKLEEKGLKAILVFGIPKKKDKYGSSAYDPNGIVQKS
                     VKLLKEETDLVVITDVCLCQYTEHGHCGIVKNKKIVNDETLKYLSKVALSHAEAGADV
                     VAPSDMMDGRVKAIREELEKNGFDDVIIMSYSAKYASSFYEPFRSAVYSSPAFGDRST
                     YQMDPPNSLEALREVKLDIDEGADIVMVKPALPYLDIIRLVKDTFGVPTAAYNVSGEY
                     SMIKAAIDANYLSNKVIIETLLSIKRAGADLIITHFAPEIVEEI"
     misc_binding    118..131
                     /gene="aladh"
                     /bound_moiety="Zn"
     misc_feature    247..251
                     /gene="aladh"
                     /note="active site"
BASE COUNT          402 a          150 c          192 g          310 t
ORIGIN      
        1 tatttctacg acttcatcag gtgtattaaa atgaaatttc catacactcg gatgagaagg
       61 ttaagaaaaa attctaaaat aaggtctcta gtgaaagaga ctactttatc caaagatgat
      121 ttgatatatc ctgtttttgt aaaagaaggt ataaaaacca aggaaaaaat tcctaaaatg
      181 ccaggacaat ataggtactc tgtcgatgaa ttaatagatg aggctaaaaa gctagaagaa
      241 aaaggattaa aagctatatt agtttttgga attccgaaaa aaaaagataa atatggatcc
      301 agcgcttatg acccaaatgg cattgtccaa aaatctgtaa aattattaaa agaagagaca
      361 gacttagtag taataaccga tgtatgtctt tgtcaatata ctgaacatgg acattgcgga
      421 atagttaaaa acaaaaaaat agtaaacgat gaaactctca aatatttatc taaagtagct
      481 ttatcacatg ctgaagctgg tgcagatgtt gttgcaccat cagacatgat ggatggtcgt
      541 gtaaaggcta taagggaaga attagaaaaa aatggttttg atgatgtaat aataatgtca
      601 tattcagcaa aatacgcgtc atctttttat gaaccattca gatcagcagt atattcatct
      661 ccagcatttg gagatagaag tacttaccaa atggatccac cgaactcttt agaagctcta
      721 agggaagtca aattagatat tgatgaaggc gccgacatcg taatggtaaa acctgcttta
      781 ccttatcttg atattattag acttgttaaa gatacttttg gagtacctac tgccgcatat
      841 aatgtaagtg gtgaatattc aatgataaaa gccgctatag atgcaaatta tctttctaat
      901 aaagttataa tagaaacatt gctttccata aaacgtgcag gtgcagattt aataatcaca
      961 cattttgctc ctgaaattgt ggaggaaata taagctatga atgctaatta tgtttcaaaa
     1021 tcagcgcaaa tagcatcaat attagaagtt agtg
//