LOCUS       ECOGADB                 1421 bp    DNA     linear   BCT 27-MAY-2008
DEFINITION  E. coli glutamate decarboxylase-beta (gadB) gene, complete cds.
ACCESSION   M84025
VERSION     M84025.1
KEYWORDS    gadB gene; glutamate decarboxylase-beta.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Smith,D.K., Kassam,T., Singh,B. and Elliott,J.F.
  TITLE     Escherichia coli has two homologous glutamate decarboxylase genes
            that map to distinct loci
  JOURNAL   J. Bacteriol. 174 (18), 5820-5826 (1992)
   PUBMED   1522060
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     gene            1..1418
                     /gene="gadB"
     5'UTR           1..17
                     /gene="gadB"
     regulatory      7..12
                     /regulatory_class="ribosome_binding_site"
                     /gene="gadB"
     CDS             18..1418
                     /gene="gadB"
                     /EC_number="4.1.1.15"
                     /standard_name="'E. coli GAD-1'"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase-beta"
                     /protein_id="AAA23834.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT          339 a          371 c          379 g          332 t
ORIGIN      
        1 attttaagga gtttaaaatg gataagaagc aagtaacgga tttaaggtcg gaactactcg
       61 attcacgttt tggtgcgaag tctatttcca ctatcgcaga atcaaaacgt tttccgctgc
      121 acgaaatgcg cgacgatgtc gcattccaga ttatcaatga cgaattatat cttgatggca
      181 acgctcgtca gaacctggcc actttctgcc agacctggga cgacgaaaat gtccacaaat
      241 tgatggattt atccattaac aaaaactgga tcgacaaaga agaatatccg caatccgcag
      301 ccatcgacct gcgttgcgta aatatggttg ccgatctgtg gcatgcgcct gcgccgaaaa
      361 atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg ctcggcggga
      421 tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca acggataaac
      481 caaacctggt gtgcggtccg gtacaaatct gctggcataa attcgcccgc tactgggatg
      541 tggagctgcg tgagatccct atgcgccccg gtcagttgtt tatggacccg aaacgcatga
      601 ttgaagcctg tgacgaaaac accatcggcg tggtgccgac tttcggcgtg acctacactg
      661 gtaactatga gttcccacaa ccgctgcacg atgcgctgga taaattccag gccgataccg
      721 gtatcgacat cgacatgcac atcgacgctg ccagcggtgg cttcctggca ccgttcgtcg
      781 ccccggatat cgtctgggac ttccgcctgc cgcgtgtgaa atcgatcagt gcttcaggcc
      841 ataaattcgg tctggctccg ctgggctgcg gctgggttat ctggcgtgac gaagaagcgc
      901 tgccgcagga actggtgttc aacgttgact acctgggtgg tcaaattggt acttttgcca
      961 tcaacttctc ccgcccggcg ggtcaggtaa ttgcacagta ctatgaattc ctgcgcctcg
     1021 gtcgtgaagg ctataccaaa gtacagaacg cctcttacca ggttgccgct tatctggcgg
     1081 atgaaatcgc caaactgggg ccgtatgagt tcatctgtac gggtcgcccg gacgaaggca
     1141 tcccggcggt ttgcttcaaa ctgaaagatg gtgaagatcc gggatacacc ctgtatgacc
     1201 tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc ggcggtgaag
     1261 ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg gactttgctg
     1321 aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac ccgaaactgc
     1381 agggtattgc ccaacagaac agctttaaac atacctgata a
//