LOCUS STMTCREP 13123 bp DNA linear BCT 26-MAY-1995 DEFINITION Streptomyces glaucescens tcm operon. ACCESSION M80674 VERSION M80674.1 KEYWORDS O-methyltransferase; acyl carrier protein; hydroxylase; polyketide cyclase /O-methyltransferase; tcm operon; tcmA gene; tcmG gene; tcmH gene; tcmI gene; tcmJ gene; tcmK gene; tcmL; tcmM gene; tcmN gene; tcmO gene; tcmP gene; tcmR gene; tetracenomycin C resistance and export gene repressor; tetracenomycin C resistance and export protein; tetracenomycin polyketide synthase condensing enzyme. SOURCE Streptomyces glaucescens ORGANISM Streptomyces glaucescens Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces. REFERENCE 1 (bases 1 to 13123) AUTHORS Summers,R.G., Wendt-Pienkowski,E., Motamedi,H. and Hutchinson,C.R. TITLE Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase JOURNAL J. Bacteriol. 174 (6), 1810-1820 (1992) PUBMED 1548230 REFERENCE 2 (sites) AUTHORS Guilfoile,P.G. and Hutchinson,C.R. TITLE Sequence and transcriptional analysis of the Streptomyces glaucescens tcmAR tetracenomycin C resistance and repressor gene loci JOURNAL J. Bacteriol. 174 (11), 3651-3658 (1992) PUBMED 1592819 REFERENCE 3 (bases 1 to 13123) AUTHORS Shen,B. and Hutchinson,C.R. TITLE Triple hydroxylation of tetracenomycin A2 to tetracenomycin C in Streptomyces glaucescens. Overexpression of the tcmG gene in Streptomyces lividans and characterization of the tetracenomycin A2 oxygenase JOURNAL J. Biol. Chem. 269 (48), 30726-30733 (1994) PUBMED 7982994 COMMENT Original source text: Streptomyces glaucescens DNA; Streptomyces glaucescens DNA; and Streptomyces glaucescens DNA. FEATURES Location/Qualifiers source 1..13123 /organism="Streptomyces glaucescens" /mol_type="genomic DNA" /db_xref="taxon:1907" gene complement(59..742) /gene="tcmR" CDS complement(59..>742) /gene="tcmR" /function="repressor of tetracenomycin C resistance and export gene" /codon_start=1 /transl_table=11 /product="tetracenomycin C resistance and export gene repressor" /protein_id="AAA67508.1" /translation="RVDSAETDTPSTRSTPNGPGLRQRKLRRTRDQLIREALELFLAQ GYEHTTVEQIAEAVEVHPRTFFRHFASKEEVALTPISAIDEAFLAALEVRPAGENPLQ AMSGAFRAVLGRVRDGELEGVDGALHMAMMRLVERTPGLLAEYLRRSEEMEGRLARII AAREGVDLDDDFRPRFIVAVFKAVGRVVSREWYLRADTDLEALSVAFESALDSLRPEL FADWRRPGA" regulatory complement(745) /regulatory_class="promoter" /gene="tcmR" /note="p1 promoter of tcmR repressor gene, apparently inducible" regulatory 820 /regulatory_class="promoter" /gene="tcmA" regulatory complement(848) /regulatory_class="promoter" /gene="tcmR" /note="p2 promoter of tcmR repressor gene, apparently constitutive" gene 878..2557 /gene="tcmA" CDS 878..2494 /gene="tcmA" /codon_start=1 /transl_table=11 /product="tetracenomycin C resistance and export protein" /protein_id="AAA67509.1" /translation="MSTETHDEPSGVAHTPASGLRGRPWPTLLAVAVGVMMVALDSTI VAIANPAIQQDLHASLADVQWITNGYLLALAVSLITAGKLGDRFGHRQTFLVGVAGFA VTSAAIGLSGSVAAIVVFRVLQGLFGALMQPSALGLLRVTFPPGKLNMAIGIWSGVVG ASTAAGPIIGGLLVQHVGWEAVFFINVPVGLAALVAGLVILTDARAERAPKSFDVSGI VLLSGAMFCLVWGLIKAPAWGWGDLRTLGFLAAAVLAFAGFTLRESRATEPLMPLAMF RSVPLSAGTVLMVLMAFSFIGGLFFVTFYLQNVHGMSPVESGVHLLPLTGMMIVGAPV SGIVISRFGPGGPLVVGMLLTAASLWGMSTLEADSGMGITSLWFVLLGLGLAPVMVGT TDVIVSNAPAELAGVAGGLQQSAMQVGGSLGTAVLGVLMASRVGDVFPDKWAEANLPR VGPREAAAIEDAAEVGAVPPAGTLPGRHAGTLSEVVHSSFISGMGLAFTVAGAVALVA AAVALFTRKAEPDERAPEEFPVPASTAGRG" regulatory 2522..2557 /regulatory_class="terminator" /gene="tcmA" /note="putative transcription terminator; putative" gene complement(2609..3424) /gene="tcmP" CDS complement(2609..>3424) /gene="tcmP" /function="putative O-methyltransferase for conversion of tetracenomycin E to tetracenomycin A2" /codon_start=1 /transl_table=11 /product="O-methyltransferase" /protein_id="AAA67510.1" /translation="PMGETIRLTGVHETLLATLQARALDNRQPRPVLGDATAEELLRR IDYDFSRIRTATKDMRIVTFRARKLDEWAGRFLAVHPDAVVLHLACGLDSRAFRMDVP DTVEWIDVDVPDVIELRSRLYPERDGYRMIGASVTTEDWLDTVPRNRPALVVAEGLTP YLREADGEEMLRRVIRHLPTGAVMFDAVLPWTLRFAKYSQLLRATGATFGWGIGNPRS LESRVPGLRFQEQWSMLDSPFLADARPVEKWVGAGMRSIPQLRFAHRLLRYTF" gene 3954..5639 /gene="tcmG" CDS 3954..5639 /gene="tcmG" /function="putative hydroxylase for conversion of tetracenomycin A2 to tetracenomycin C" /codon_start=1 /transl_table=11 /product="hydroxylase" /protein_id="AAA67511.1" /translation="MSTEEVPVLIVGGGLTGLSAALFLSQHGVSCRLVEKHRGTTVLT RASGISSRTMELLRGVGLERTVIERGPKLVEGARWRELGQPADQIPWVVIRANGLHDL ENAVTVEEPSLDVGHLSPTRPYWCGQDRLEPILRDEAVRRGARIDFNTRMEAFTADES GVTATIVDQATGEQSTVRARYLIAADGVRSPVRETLGITRTGHGTIGNAMSVLFKADL RDTVKGRRFVICYLPNPDEPGVLQLPEVPAVLQLFDEDRWIFGFFFDPRETSPEQFTD ERCAQIIRTATGLPGLPVEVQMARPWEMSHNSARSYRSGRVFLAGDAAHVHPPAGAFG ANGGIQDAHNLAWKLAAVLKGTAGDALLDTYEQERLPIGAAVADQAWIRHTWRLNDSE ELRRALRESTLVATGYRYTSDAVLGAAYPEPIPAAHDLTGRPGYRVPHVWLGRGGERV STVDLGGDAFVVLAGPDGGEWQAAADKVAQDLGVPVHCHPVGGDGQLTDPDGAFLGTT GLTRNGALLIRPDGFVAWRAEYLPEDAAGELRSALERILARTSGTPGGTALEG" protein_bind 3987..4052 /gene="tcmG" /note="binding site in TcmG protein; putative" /bound_moiety="FAD" gene 5644..5982 /gene="tcmH" CDS 5644..5982 /gene="tcmH" /function="required for conversion of tetracenomycin F2 to tetracenomycin D3; putative hydroxylase" /codon_start=1 /transl_table=11 /product="hydroxylase" /protein_id="AAA67512.1" /translation="MATISPSPDLFTLVNVFGVAPEKQRELRDHLVQVTEDLIRHMPG FVSATFHLSRDGEQVVNYAQWRSEADFRAMHADPRLQPHFDYCRSVSRPKPIFCEVTH SFGATSPEGA" gene 5985..6314 /gene="tcmI" CDS 5985..6314 /gene="tcmI" /function="required for conversion of tetracenomycin F2 to tetracenomycin D3" /codon_start=1 /transl_table=11 /protein_id="AAA67513.1" /translation="MAYRALMVLRMDPADAEHVAAAFAEHDTTELPLEIGVRRRVLFR FHDLYMHLIEADDDIMERLYQARSHPLFQEVNERVGQYLTPYAQDWEELKDSKAEVFY SWTAPDS" gene 6328..6777 /gene="tcmJ" CDS 6328..6777 /gene="tcmJ" /function="possibly required for conversion of tetracenomycin F2 to tetracenomycin D3" /codon_start=1 /transl_table=11 /protein_id="AAA67514.1" /translation="MTTAQHEGEQTVAAAAKIAMSSVAPSHRQGGSTRALLTPSSVGA TSGFLGHIELAPGESVTEHYHPFSDKYLYLIEGSLVVRVNGEEVRLERDEALFVTRGQ RHRIENRGNVPARVVFQISPLAPRPELGHVDTEPVPNPAAAPPKVGG" gene 6779..8059 /gene="tcmK" CDS 6779..8059 /gene="tcmK" /note="putative; putative" /codon_start=1 /transl_table=11 /product="tetracenomycin polyketide synthase condensing enzyme" /protein_id="AAA67515.1" /translation="MTRHAEKRVVITGIGVRAPGGAGTAAFWDLLTAGRTATRTISLF DAAPYRSRIAGEIDFDPIGEGLSPRQASTYDRATQLAVVCAREALKDSGLDPAAVNPE RIGVSIGTAVGCTTGLDREYARVSEGGSRWLVDHTLAVEQLFDYFVPTSICREVAWEA GAEGPVTVVSTGCTSGLDAVGYGTELIRDGRADVVVCGATDAPISPITVACFDAIKAT SANNDDPAHASRPFDRNRDGFVLGEGSAVFVLEELSAARRRGAHAYAEVRGFATRSNA FHMTGLKPDGREMAEAITAALDQARRTGDDLHYINAHGSGTRQNDRHETAAFKRSLGQ RAYDVPVSSIKSMIGHSLGAIGSLELAACALAIEHGVIPPTANYEEPDPECDLDYVPN VAREQRVDTVLSVGSGFGGFQSAAVLARPKETRS" gene 8056..9285 /gene="tcmL" CDS 8056..9285 /gene="tcmL" /function="required for tetracenomycin polyketide synthase; function unknown" /codon_start=1 /transl_table=11 /protein_id="AAA67516.1" /translation="MSAPAPVVVTGLGIVAPNGTGTEEYWAATLAGKSGIDVIQRFDP HGYPVRVGGEVLAFDAAAHLPGRLLPQTDRMTQHALVAAEWALADAGLEPEKQDEYGL GVLTAAGAGGFEFGQREMQKLWGTGPERVSAYQSFAWFYAVNTGQISIRHGMRGHSSV FVTEQAGGLDAAAHAARLLRKGTLNTALTGGCEASLCPWGLVAQIPSGFLSEATDPHD AYLPFDARAAGYVPGEGGAMLVAERADSARERDAATVYGRIAGHASTFDARPGTGRPT GPARAIRLALEEARVAPEDVDVVYADAAGVPALDRAEAEALAEVFGPGAVPVTAPKTM TGRLYAGGAALDVATALLSIRDCVVPPTVGTGAPAPGLGIDLVLHQPRELRVDTALVV ARGMGGFNSALVVRRHG" gene 9358..9609 /gene="tcmM" CDS 9358..9609 /gene="tcmM" /function="acyl carrier protein for tetracenomycin polyketide synthase" /codon_start=1 /transl_table=11 /product="acyl carrier protein" /protein_id="AAA67517.1" /translation="MPQIGLPRLVEIIRECAGDPDERDLDGDILDVTYQDLGYDSIAL LEISAKLEQDLGVSIPGEELKTPRHTLHLVNTETAGEVA" gene 9611..11149 /gene="tcmN" CDS 9611..11095 /gene="tcmN" /function="O-methyltransferase" /function="putative polyketide cyclase" /note="possible internal translational start site at position 9950; SAM binding site at position 10617 to 10636" /codon_start=1 /transl_table=11 /protein_id="AAA67518.1" /translation="MAARTDNSIVVNAPFELVWDVTNDIEAWPELFSEYAEAEILRQD GDGFDFRLKTRPDANGRVWEWVSHRVPDKGSRTVRAHRVETGPFAYMNLHWTYRAVAG GTEMRWVQEFDMKPGAPFDNAHMTAHLNTTTRANMERIKKIIEDRHREGQRTPASVLP TELHAQQLLLLAASGRLARIVHVLTELRIADLLADGPRHVAELAKETDTHELSLYRVL RSAASVGVFAEGPVRTFSATPLSDGLRTGNPDGVLPLVKYNNMELTRRPYDEIMHSVR TGEPAFRRVFGSSFFEHLEANPEAGEFFERFMAHWSRRLVLDGLADQGMERFSRIADL GGGDGWFLAQILRRHPHATGLLMDLPRVAASAGPVLEEAKVADRVTVLPGDFFTDPVP TGYDAYLFKGVLHNWSDERAVTVLRRVREAIGDDDARLLIFDQVMAPENEWDHAKLLD IDMLVLFGGRERVLAEWRQLLLEADFDIVNTPSHTWTTLECRPV" regulatory 11121..11149 /regulatory_class="terminator" /gene="tcmN" /note="potential; putative" gene 11192..12211 /gene="tcmO" CDS 11192..12211 /gene="tcmO" /function="putative O-methyltransferase for conversion of tetracenomycin B3 to tetracenomycin E" /codon_start=1 /transl_table=11 /product="O-methyltransferase" /protein_id="AAA67519.1" /translation="MTPHTHVRGPGDILQLTMAFYGSRALISAVELDLFTLLAGKPLP LGELCERAGIHPRGARDFLDALVALGLLEREGEDTYRNSPAADRHLDRRKPGYVGGYA RLADTKLFPVWARLTEALRTGEKQVPSQGGFFGGYADPEAARGFLGAMDAVNGGVGHS LAGALDWTEYSSFVDLGGARGNLAAHLHRAHPHLRATCFDLPEMEPFFQEHMKSLETT DQVRFAGGDFFTDPLPRADVFIVGHILHYFGLRQREALIARIHQALTPGGAVLVYDRM IDDDRRSAALSLLGSLNMLLTSDEGREYTPAECVRWLSDAGFTDVRTTAVSGPDTLAI GRKPR" BASE COUNT 1951 a 4793 c 4520 g 1859 t ORIGIN 1 gcggccgccc cctcgggggc ctggcgcccc gtcagccacc cgggggtctg acgctccgtc 61 aggcccccgg gcgccgccag tcggcgaaca actccggccg cagcgagtcc agagcggact 121 cgaacgccac ggagagcgcc tccagatccg tgtcggcgcg caggtaccac tcccggctga 181 cgacgcggcc gaccgccttg aacaccgcca cgatgaaccg cggacggaag tcgtcgtcca 241 ggtcgacgcc ctcacgcgcg gcgatgatcc gggcgagtcg gccctccatc tcctcgctgc 301 gccgcaggta ctccgcgagg agcccggggg tgcgctcgac cagccgcatc atcgccatgt 361 gcaacgcgcc gtccacgccc tccagttcac cgtcgcgcac gcgcccaagg acggcccgga 421 aggcaccgct catggcctgc agcgggttct cgcccgccgg ccggacctcc agcgcggcga 481 ggaacgcctc gtcgatggcg ctgatggggg tgagggccac ctcctccttg ctggcgaagt 541 ggcggaagaa cgtgcgcgga tgcacctcga ccgcctcggc gatctgctcc acggtcgtgt 601 gctcgtaacc ctgcgccagg aacagctcca gggcctcgcg gatcagctga tcacgcgtac 661 ggcgcagctt ccgctggcgc aggccggggc cgttgggggt gctgcgggtg ctgggggtgt 721 cggtttcggc ggagtccacc cgccaaccct atcttgcgcc acctggtgac acctgcatgc 781 gattgtcaaa tgtcactgac tgctgttagc ttcggggcca tgttctcctc gtcacacacg 841 cgtccgtcct gccggaaatc gttaggggga cgctgaaatg agcacggaaa cgcacgacga 901 gccgtcaggc gtcgcacaca cccccgcttc cggtctgcgt ggacgtccct ggccgaccct 961 gctcgcggtc gcggtcggcg tgatgatggt ggctctggac agcaccatcg tcgccatcgc 1021 caacccggcc atccagcagg acctccacgc ctcgctggcc gatgtgcagt ggatcaccaa 1081 cggctacctg ctggcgctcg cggtctccct gatcaccgcc ggcaaactgg gcgacaggtt 1141 cgggcaccgg cagaccttcc tggtcggagt ggcgggcttc gccgtcacct ccgcggccat 1201 cggcctgtcc ggcagcgtcg cggcgatcgt cgtcttccgc gttctccagg gcctgttcgg 1261 cgctctgatg cagccgtcgg cgctgggcct gctgcgggtc acgttcccgc ccggcaagct 1321 caacatggcg atcggcatct ggagcggcgt ggtcggcgcc tcgaccgcag ccggaccgat 1381 catcggcggg ctcctggtgc agcacgtcgg ctgggaggcg gtcttcttca tcaatgtgcc 1441 ggtcggcctc gcggcactcg tcgccggcct cgtcatcctg accgacgcgc gcgccgaacg 1501 cgccccgaag tccttcgacg tctccggcat cgtgctcctg tccggggcca tgttctgcct 1561 ggtgtggggt ctgatcaagg ctccggcctg gggctggggc gacctgcgga cgctgggctt 1621 cctggccgcc gccgtgctcg ccttcgccgg attcacactg cgggagagca gggccaccga 1681 gccgctgatg ccgctcgcca tgttccgttc cgtgccgctg tccgcgggca ccgtgctcat 1741 ggtcctgatg gcgttctcgt tcatcggcgg actcttcttc gtcaccttct acctgcagaa 1801 cgtccatggc atgagcccgg tcgagagcgg tgtgcacctg ctgccgctca ccggcatgat 1861 gatcgtcggc gcccccgtgt ccggcatcgt gatcagccgg ttcggaccag gcgggccgct 1921 ggtggtgggc atgctcctga cggcggcgtc cctgtggggg atgtcgacgc tggaagcgga 1981 cagcggtatg gggatcacct cgctctggtt cgtgctgctg ggtctcggtc tcgcgccggt 2041 gatggtgggg acgacggacg tcatcgtcag caacgccccg gccgaactgg ccggtgtggc 2101 cggtggtctt cagcagtccg cgatgcaggt cggcggaagt ctcggcaccg ccgtactcgg 2161 tgtcctcatg gcctcccggg tcggcgacgt cttccccgac aagtgggcgg aggcgaacct 2221 gccgcgggtg ggtccgcggg aggccgcggc gatcgaggac gcggccgagg tgggagccgt 2281 cccgccggcc ggcaccctcc ccggccggca cgccgggacg ctctccgagg tggtccactc 2341 ctcgttcatc tcggggatgg ggctggcctt caccgtggcc ggtgccgtcg cgctcgtggc 2401 cgccgccgtc gccctgttca ccaggaaggc cgagcccgac gagagggccc cggaggaatt 2461 cccggtcccg gcgtccacgg cagggcgggg ctgaaagcct cggtccctgt ccgccggtga 2521 atgccggcgg ggtccggtcc ggcggacccc gccggcaccg ctgtttccgc gatgcccgaa 2581 cggaggggac ggaggaaaag tcccggaatc agaaggtgta acgcagcagc cggtgtgcga 2641 aacgcagttg cggaatgctg cgcatgccgg ctcccaccca tttctcgacg gggcgggcgt 2701 cggcgagaaa cggggaatcg agcatggacc actgctcctg gaaccgtaat cccggcaccc 2761 tgctctccag ggagcgggga tttccgattc cccatccgaa ggtggcgccc gtggcgcgca 2821 gaagttgtga atatttggcg aatcgaaggg tccacgggag tacggcgtcg aacatgaccg 2881 cacccgtcgg aaggtgccgg atcacccgcc ggagcatttc ctctccgtct gcttcccgga 2941 gatagggcgt cagcccttcg gcgaccacga gcgccggccg gttgcggggc accgtgtcca 3001 gccagtcctc cgtggtgacc gacgctccga tcatccggta tccgtcgcgt tcgggataga 3061 gccggctcct cagttcgatg acgtccggga cgtcgacgtc gatccattcc actgtgtcgg 3121 gcacgtccat ccggaatgcc cggctgtcca gcccgcatgc gaggtgcagc accaccgcgt 3181 ccggatggac cgcgaggaat cgaccggccc actcgtcgag cttcctggcg cggaacgtca 3241 cgatccgcat gtccttggtg gctgttctga tgcggctgaa gtcgtagtcg atgcggcgca 3301 gcagctcctc cgccgtcgcg tcccccagca cgggtcgcgg ttgtcgattg tccagggcac 3361 gggcctgaag tgtggcgagc agcgtctcgt gtacgccggt cagccggatc gtttcaccca 3421 tgggacagca gcttccgagg gggcaccgcg tgacgtcaag agaccccctc ggcgggccgt 3481 ggcgaaaaga tctggacaca ttgccgtacg caaagtcggg acacttccag gtattcctcg 3541 ccaaacagcg ggagcgttcg gccaggcgct tggctcgatc tgatcaacat gtcggaggga 3601 ggcgccgtcg cggacggcct gatctttgtc gtcggaaagg taaggggatc gccaagaggt 3661 aaaggattca tagggcatgt tcaaaagcat cggtcatcaa aaagggggtt cgtgtttaac 3721 tccggagagt cgagggtata catgcccatc aattttccgg tccacttctg accgagtgtc 3781 agtgacatct atccggtctc cccgccgaag attgttcgat gttgactcga cggaccggaa 3841 gatgttcatc tggatgagcc aagtgccgct ctgctcgtcg gcccgagcaa atccccgatc 3901 tcatcgcagg gatgactcgt gtgcccgttt ccgaccgacc gaaaggctgc atcttgtcca 3961 ctgaagaagt tccggtactg atagtcggcg gaggtcttac cggactgtcc gcggcactgt 4021 tcctcagtca gcacggagtt tcctgccgcc tggtggagaa gcaccgggga accacggtcc 4081 tgacccgggc ctcggggatc agctcccgca cgatggaact gttgcggggc gtcggcctgg 4141 aacggaccgt gatcgagcgc gggccgaaac tcgtcgaggg agcccgctgg cgggagctcg 4201 gccagccggc ggaccagatc ccctgggtcg tcatccgggc caacggcctg cacgacctgg 4261 agaacgcggt gaccgtcgag gaaccctcgc tcgacgtcgg ccacctcagc cccacccgcc 4321 cctactggtg cgggcaggac cggctggaac ccatcctccg cgacgaggcc gtccgccgtg 4381 gggcccgcat cgacttcaac acccgcatgg aggcgttcac ggcggacgag agcggggtca 4441 ccgccacgat cgtcgaccag gccaccggcg agcagagcac ggtccgcgcc cggtacctga 4501 tcgcggcgga cggggtgcgc agcccggtgc gggagacgct ggggatcacg cgcaccgggc 4561 acggcacgat cggcaacgcc atgagcgtcc tgttcaaggc ggacctgcgg gacaccgtca 4621 agggccggcg cttcgtcatc tgctacctgc cgaaccccga cgagcccggc gtcctccagc 4681 tcccggaggt gccggccgtc ctccagctct tcgacgagga ccgctggatc ttcggcttct 4741 tcttcgaccc ccgcgagacc tcgcccgagc agttcaccga cgagcgctgc gcgcagatca 4801 tccggaccgc gaccgggctc cccggcctcc cggtcgaggt gcagatggcc aggccctggg 4861 agatgtccca caacagtgcc cgctcctacc gcagcggccg ggtcttcctg gccggcgacg 4921 ccgcccacgt gcacccaccg gccggtgcct tcggagcgaa cggcggtatc caggacgccc 4981 acaacctggc ctggaagctg gcggccgtcc tgaagggaac ggccggcgac gccctgctcg 5041 acacctacga gcaggaacgc ctgccgatcg gtgccgccgt cgccgaccag gcgtggatcc 5101 ggcacacctg gaggctgaac gactccgagg agctgcgccg cgcgctgcgc gagtcgacgc 5161 tggtcgccac cggttaccgg tacacctccg acgcggtcct cggtgccgcc tacccggagc 5221 ccatccccgc ggcgcacgac ctcaccggcc ggccggggta ccgcgtgccc cacgtctggc 5281 tgggccgggg cggggagcgc gtctcgacgg tcgacctcgg cggggacgcg ttcgtcgtgc 5341 tcgccgggcc ggacggaggc gagtggcagg ccgcggccga caaggtcgcg caggacctgg 5401 gcgtgccggt gcactgccac ccggtgggcg gcgacgggca actgaccgat cccgacggcg 5461 cgttcctcgg caccacgggc ctcacccgga acggggcgct gctgatccgg ccggacggct 5521 tcgtggcgtg gcgggccgag tacctgccgg aggacgccgc cggggagctg cggtccgcac 5581 tggagcggat cctggcgcgg acctccggca cgcccggcgg cacggcgctg gagggctgac 5641 cgcatggcga ccatctcccc ctcgccggac ctgttcaccc tcgtcaacgt cttcggcgtc 5701 gcccccgaga agcagcggga actgcgcgac cacctggtgc aggtgaccga ggacctgatc 5761 cggcacatgc cgggtttcgt ctccgcgacc ttccacctca gccgcgacgg cgaacaggtc 5821 gtcaactacg cacagtggcg gtcggaggcc gacttccgcg ccatgcacgc cgacccgcgg 5881 ctgcagcccc acttcgacta ctgccggtcc gtgtcccggc cgaagccgat cttctgcgag 5941 gtcacccact ccttcggcgc cacgtcaccg gagggagcgt gaccatggcc taccgggcac 6001 tgatggtcct gcgcatggac ccggcggacg cggagcacgt ggcagccgcc ttcgccgagc 6061 acgacacgac cgaactgccg ctggagatcg gggtgcgacg gcgggtcctg ttccgcttcc 6121 acgatctgta catgcacctc atcgaggccg acgacgacat catggagcgg ctctaccagg 6181 cgcgcagcca cccgctgttc caggaggtca acgagcgcgt ggggcagtac ctcacgccgt 6241 acgcgcagga ctgggaggag ctgaaggact ccaaggcgga ggtcttctac agctggaccg 6301 cccccgactc ctgaccgccg gccatccgtg accaccgctc agcacgaagg agaacagacc 6361 gtggcagccg ccgccaagat cgccatgtcc agtgtggcgc ccagtcaccg gcaggggggc 6421 agcacccgcg ccctgctgac gccgtcgtcg gtgggtgcga cctccggatt cctcggccac 6481 atcgaactgg cgcccgggga gtccgtcacc gagcactacc acccgttctc ggacaagtac 6541 ctgtacctga tcgaagggag cctcgtggtc cgggtgaacg gcgaggaggt gcgcctggag 6601 cgggacgagg ccctgttcgt gacccggggc cagcggcacc gcatcgagaa ccgaggaaac 6661 gttcctgcac gggtggtctt ccagatctcg ccgctcgccc cgcggcccga actcggccat 6721 gtcgacaccg agcccgtgcc gaacccggcg gcggcgccgc cgaaggtggg aggctgagat 6781 gacccggcac gcggagaagc gggtggtgat caccggcatc ggtgtgcgag ccccgggagg 6841 cgccggaacc gcggccttct gggacctgct caccgccgga cgcaccgcca ccaggacgat 6901 ctccctgttc gacgccgcgc cctaccggtc ccggatagcc ggagagatcg acttcgaccc 6961 gatcggcgag ggcctgtcgc cccggcaggc gtcgacgtac gaccgggcca cgcagctcgc 7021 cgtcgtctgc gcccgcgagg cgctgaagga cagcgggctg gacccggccg cggtgaaccc 7081 ggaacggatc ggggtgagca tcggcaccgc cgtcggctgc accaccggcc tcgaccgcga 7141 gtacgcgcgg gtcagcgagg gcggctcgcg ctggctggtc gaccacacgc tcgcggtgga 7201 gcagctcttc gactacttcg tcccgacctc catctgccgg gaggtcgcct gggaggcggg 7261 ggccgaggga ccggtgacgg tggtgtcgac cgggtgcacc tcgggcctcg acgccgtcgg 7321 atacggcacg gagctcatcc gggacgggcg cgccgacgtg gtcgtctgcg gcgccaccga 7381 cgcgccgatc tcccccatca ccgtggcctg cttcgacgcc atcaaggcga cgtcggccaa 7441 caacgacgac ccggcccacg cctcccgccc cttcgaccgc aaccgcgacg gcttcgtgct 7501 cggggagggc tcggcggtct tcgtcctgga ggagctgtcc gccgcccgcc ggcgaggagc 7561 gcacgcctac gcggaggtcc gcggcttcgc cacacggtcg aacgccttcc acatgaccgg 7621 tctgaagccg gacggcaggg agatggcgga ggccatcacc gccgccctgg accaggcccg 7681 ccgcaccggc gacgacctgc actacatcaa cgcgcacggc tccggcaccc ggcagaacga 7741 ccggcacgag acggcggcct tcaagcgcag cctggggcaa cgggcgtacg acgtgcccgt 7801 cagctccatc aagtccatga tcggccactc gctcggagcg atcggctccc tcgaactggc 7861 cgcctgcgcg ctggcgatcg aacacggcgt gatcccgccc accgccaact acgaggaacc 7921 cgaccccgaa tgcgacctcg actacgtgcc gaacgtcgcg cgggaacagc gggtcgacac 7981 cgtgctcagc gtcggcagcg gattcggcgg cttccagagc gccgcggtgc tggcccggcc 8041 gaaggagacc cgctcatgag cgcgccggcc cccgtcgtgg tcaccggcct gggcatcgtg 8101 gcacccaacg gcaccggcac cgaggagtac tgggcggcca cgctcgccgg caagagcggc 8161 atcgacgtca tccagcggtt cgacccccac ggctaccccg tccgggtggg aggcgaggtg 8221 ctggccttcg acgccgccgc ccatctcccc gggcgcctgc tgccgcagac cgaccgcatg 8281 acccagcacg ccctggtggc cgccgaatgg gccctggccg acgccgggct ggagcccgag 8341 aagcaggacg agtacggcct cggcgtcctg acggccgccg gcgcgggagg gttcgagttc 8401 ggccagcgcg agatgcagaa actctgggga acgggcccgg aacgcgtcag cgcctaccag 8461 tcgttcgcct ggttctacgc ggtgaacacc ggccagatct ccatccgcca cggcatgcgc 8521 gggcacagct ccgtgttcgt caccgagcag gccggcggtc tcgacgccgc cgcccacgcg 8581 gcccgcctgc tgcgcaaggg gaccctgaac accgccctga ccggcggatg cgaagcctcc 8641 ctctgcccct ggggcctggt cgcccagatc ccgagcggct tcctgagcga ggccaccgac 8701 ccgcacgacg cctacctgcc cttcgacgcg cgggcggccg ggtacgtgcc cggcgagggc 8761 ggagccatgc tggtcgccga gcgggcggac tccgcacgcg aacgggacgc cgccacggtc 8821 tacgggagga tcgccgggca cgcctccacc ttcgacgccc gccccggcac cggccggccc 8881 accgggccgg cccgggccat ccgcctggcc ctcgaagagg cccgggtcgc gcccgaggac 8941 gtcgacgtgg tctacgccga cgccgctgga gtgccggccc tcgaccgcgc ggaggcggag 9001 gccctcgccg aggtcttcgg gcccggggcc gtaccggtca ccgcgcccaa gacgatgacc 9061 ggacggctct acgccggggg agcggccctc gacgtcgcga ccgcgctgct gtccatcagg 9121 gactgtgtcg tcccgccgac cgtcggcacc ggtgcgcccg cgccggggct cggcatcgac 9181 ctggtgctcc accagccccg tgagctgcgg gtggacaccg ccctggtcgt cgcgcggggc 9241 atgggcggat tcaactccgc cctggtcgtc cggcgccacg gctgaccgag ccgccgcccc 9301 cgctctccgc gcccgagcgc gcatcacctc atcacaccac tcatccggag gttcaccgtg 9361 ccccagatcg gcctgccccg actcgtcgag atcatcaggg aatgcgcagg agaccccgac 9421 gaacgggacc tggacggcga catcctcgac gtcacctacc aggacctcgg ctacgactcc 9481 atcgccctgc tggagatctc cgcgaagctg gaacaggacc tcggggtgtc gataccgggg 9541 gaggagctga agaccccccg gcacaccctc cacctcgtca acaccgagac ggccggggag 9601 gtcgcctgac atggcagcgc gcacggacaa ctccatcgtc gtcaacgcgc cgttcgagct 9661 cgtctgggac gtcaccaacg acatcgaggc atggccggag ctgttctccg agtacgccga 9721 ggcggagatc ctccggcagg acggcgacgg cttcgacttc cggctcaaga cccgccccga 9781 cgcgaacgga agggtctggg agtgggtctc ccaccgtgtg cccgacaagg gctcccgcac 9841 ggtccgggcg caccgcgtcg agaccgggcc cttcgcctac atgaacctgc actggaccta 9901 ccgggccgtg gccgggggca ccgagatgcg gtgggtgcag gagttcgaca tgaagcccgg 9961 ggccccgttc gacaacgcgc acatgaccgc tcacctcaac acgacgacac gggcgaacat 10021 ggagcgcatc aagaagatca tcgaggacag gcaccgggaa ggacagcgga ccccggcgtc 10081 cgtcctgccc accgaactgc acgcccagca actgctgttg ctggccgcct ccgggcgcct 10141 ggcgcggatc gtccacgtgc tgaccgagct gaggatcgcc gatctcctcg ccgacggccc 10201 ccgtcacgtc gcggaactgg ccaaggagac ggacacccac gagctctccc tctaccgcgt 10261 cctgcgcagc gccgcctccg tcggcgtctt cgcggagggg cccgtcagga ccttctccgc 10321 gacgcccctg tcggacggac tgcgcaccgg caacccggac ggtgtgctgc ccctggtgaa 10381 gtacaacaac atggagctga ccaggcggcc ctacgacgag atcatgcaca gtgtgcgcac 10441 cggcgaaccg gccttccggc gggtgttcgg cagctcgttc ttcgagcacc tggaggccaa 10501 cccggaagcc ggcgagttct tcgagcggtt catggcccac tggagccgcc ggctcgtcct 10561 cgacggactc gccgaccagg gcatggagcg cttctcgcgc atcgcggacc tcggcggtgg 10621 cgacggctgg ttcctcgccc agatcctccg gcgccacccg cacgccacgg gcctgctcat 10681 ggacctcccg cgggtggccg cgtcggccgg ccccgtcctg gaggaggcca aagtcgccga 10741 ccgggtcacc gtgctgcccg gcgacttctt cacggacccc gtccccaccg gctacgacgc 10801 ctacctcttc aagggcgtgc tgcacaactg gtccgacgag cgggccgtga ccgtgctgcg 10861 ccgggtccgg gaggccatcg gcgacgacga cgcacgcctg ctgatcttcg accaggtcat 10921 ggcccccgag aacgagtggg accacgccaa gctgctggac atcgacatgc tggtgctctt 10981 cggaggccgc gaacgggtgc tggccgagtg gcggcagctg ctgctcgagg cggacttcga 11041 catcgtcaac acgccctccc acacctggac cacgctggag tgccgccccg tctagggcct 11101 gtccggatcg tcccggcgcc gcggggtccg gcacccggcc ggaccccgcc cgaaccgcca 11161 gcccccacgt tcaaggagag ccgagcacac catgacaccg cacacgcacg tccgcggacc 11221 gggcgacatc ctccagctga ccatggcctt ctacggatcg cgcgccctca tcagcgcggt 11281 cgaactggac ctgttcaccc tgctcgccgg gaaaccgctg ccgctcggcg agctgtgcga 11341 gcgggcgggc atccatcctc gcggcgcgcg ggacttcctc gacgccctgg tggcactggg 11401 actcctggag cgcgagggcg aggacaccta ccgcaacagc ccggccgccg accgccatct 11461 ggaccggcgc aagcccgggt acgtcggcgg ctacgcccgg ctcgcggaca ccaagctgtt 11521 ccccgtctgg gcacggctga cggaggccct gcgcacgggc gagaagcagg tgccgagcca 11581 gggcggcttc ttcggcggct acgcggaccc ggaggcggcc cgcggcttcc tcggcgccat 11641 ggacgccgtc aacggcggcg tcgggcacag cctggccggc gcgctcgact ggacggagta 11701 ctcctccttc gtcgacctcg gcggcgcgcg cggcaacctc gccgcgcacc tgcaccgggc 11761 tcacccgcac ctgcgggcca cctgtttcga cctgccggag atggaaccct tcttccagga 11821 gcacatgaag tccctggaga cgacggacca ggtgcgcttc gccgggggcg acttcttcac 11881 cgaccccctg ccgcgcgcgg acgtgttcat cgtcgggcac atcctccact acttcggtct 11941 ccggcagcgc gaggcgctga tcgcccgcat ccaccaggcg ctcaccccgg gcggtgccgt 12001 cctcgtctac gaccggatga tcgacgacga ccggcgcagt gccgcactga gcctgctcgg 12061 cagcctcaac atgctgctga cctccgacga gggacgcgag tacacccccg cggagtgcgt 12121 ccggtggctg tccgacgccg gattcaccga cgtccgcacc accgcggtga gcgggcccga 12181 caccctcgcg atcggccgca aaccccggtg aggtggctcg gcaggaccgt ctggctgggg 12241 ctgatcggcc tcggcgcgtt ctggaccggg cacctgcccc ccggcccgta cgggcaggac 12301 cgggccgggt ccgccccgcc ggccggacac ccggaacggc tcgtccccac ggcgctcacc 12361 gcccaggagc tgatcctgtc gcgcgagctg ggctggaccc gctgacctgc cgggaacccc 12421 ggcccggtgc gcggcgggca ccccggaccg gggcacgtcc gcgtccgcgg gagtgctcgg 12481 cggacgtacc ggcccgcccg gcctccccgg cggcgacgcg gcgccgtcgg gtgcgtggcc 12541 gagccgcgcg ccccgtggcc gcggccggcg ccgcggggac ctctcacggg atcccgtgcc 12601 tggccgcgcc ccggtcgggg aggcgaggac caccagcgcg cggccgggcc gcgcggtcag 12661 gtgaaggtga ccgggccgcg cggtgtgccg accgtgaagg agaacccggc cggcccctcg 12721 gcgagggcca gcccggtgtc cagggcggcc agcagggggc ggatctccgc cgggtcggga 12781 gccgtcgccg acagccgcag caagggggtg acgggcagcc cggcggcggt ggggtggcgg 12841 gaggcgcccc agtcgatgag gaagggcacc agcccggacg ggtgggcgga accgtccgtc 12901 agccgccact ccagcagggc gccgtcgggc gtgcggcggc tcatcgcccg ggcgggaccg 12961 ggatcgtggc cgcgggcgcg ggccgaggcg atcgccgcgt ccaggtcggt cgtgcggatc 13021 gcccaggtca cggtgcgcgg gccgggcagc aggtccacgc cgaacggccg cgggccggcc 13081 ggggcgggct gctccgggtc cgggccgatg atctccaggt acc //