LOCUS       ECOMIAA                 1328 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  E.coli (delta)2-isopentenyl pyrophosphate transferase (miaA) gene,
            complete cds, and mutator protein (mutL) gene, 3' end.
ACCESSION   M63655 M26956 M37459
VERSION     M63655.1
KEYWORDS    (delta)2-isopentenyl pyrophosphate transferase; IPP transferase;
            miaA gene; mutL gene; mutator protein.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (sites)
  AUTHORS   Connolly,D.M. and Winkler,M.E.
  TITLE     Genetic and physiological relationships among the miaA gene,
            2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification,
            and spontaneous mutagenesis in Escherichia coli K-12
  JOURNAL   J. Bacteriol. 171 (6), 3233-3246 (1989)
   PUBMED   2656644
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Connolly,D.M. and Winkler,M.E.
  TITLE     Structure of Escherichia coli K-12 miaA and characterization of the
            mutator phenotype caused by miaA insertion mutations
  JOURNAL   J. Bacteriol. 173 (5), 1711-1721 (1991)
   PUBMED   1999389
COMMENT     Original source text: E.coli K-12 DNA, clone pNU127.
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /db_xref="taxon:562"
     gene            1..63
                     /gene="mutL"
                     /map="94.8 min"
     CDS             1..63
                     /gene="mutL"
                     /function="required for methyl-directed mismatch"
                     /codon_start=1
                     /map="94.8 min"
                     /transl_table=11
                     /product="mutator protein"
                     /protein_id="AAA24173.1"
                     /translation="PGGLLQSVDLHPAIKALKDE"
     gene            56..1006
                     /gene="miaA"
                     /map="95 min. on K-12 map"
     CDS             56..1006
                     /gene="miaA"
                     /codon_start=1
                     /map="95 min. on K-12 map"
                     /transl_table=11
                     /product="delta-2-isopentenyl pyrophosphate transferase"
                     /protein_id="AAA24174.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     CDS             1093..1328
                     /note="ORF"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24175.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHRTTPVAVP"
BASE COUNT          317 a          297 c          382 g          332 t
ORIGIN      95 min. on K-12 map.
        1 ccgggtggtc tgttacaatc tgttgattta catccggcga taaaagccct gaaagatgag
       61 tgatatcagt aaggcgagcc tgcctaaggc gatttttttg atggggccga cggcctccgg
      121 taaaacggcg ttagccattg agctgcgtaa aattttacca gtagagttga taagcgttga
      181 ttctgccctt atttacaaag ggatggatat cgggacggcg aagccgaacg ctgaagagtt
      241 actcgccgcg ccgcaccgat tgctggatat tcgcgatccg tcgcaggctt actcggctgc
      301 tgattttcgc cgcgatgcgc tggcggaaat ggccgatatc accgcggcgg ggcggatccc
      361 actgttagtg ggcggtacga tgttgtattt caaggcattg ctggaagggt tgtcgccgct
      421 accgtcggca gacccggaag tacgggccag aattgagcaa caggcggcag agcaaggttg
      481 ggagtcattg catcgtcaac ttcaggaggt agatccggtt gcggcagcaa ggattcatcc
      541 aaatgatcca caaaggcttt cccgggcact ggaagttttt ttcatttcgg gtaaaacttt
      601 aacggaactg acgcaaacgt caggagacgc tctaccgtat caggtgcatc agttcgccat
      661 cgccccggcg agccgtgaac tgctccatca acgcattgag cagcgttttc atcagatgtt
      721 ggcttcaggt tttgaagcag aagtccgggc gctttttgcc cgaggagatt tgcatacgga
      781 cttgccttcc attcgttgcg tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat
      841 ctcatacgat gaaatggttt atcgaggtgt ttgcgccacg agacagttgg cgaagcggca
      901 gataacctgg ctgcgtggtt gggaaggggt tcactggctt gacagtgaaa aaccagaaca
      961 ggcgcgtgac gaagtattac aggttgttgg tgctatcgca ggctgaatgt gtacaattga
     1021 gacgtatcgt gcgcaatttt ttcaggaatc gaaaggttca aagtacaaat aagcatataa
     1081 ggaaaagaga gaatggctaa ggggcaatct ttacaagatc cgttcctgaa cgcactgcgt
     1141 cgggaacgtg ttccagtttc tatttatttg gtgaatggta ttaagctgca agggcaaatc
     1201 gagtcttttg atcagttcgt gatcctgttg aaaaacacgg tcagccagat ggtttacaag
     1261 cacgcgattt ctactgttgt cccgtctcgc ccggtttctc atcacagaac aacgccggtg
     1321 gcggtacc
//