LOCUS       MEFDNABP                1698 bp    DNA     linear   BCT 04-AUG-1994
DEFINITION  Methanothermus fervidus DNA binding protein (hmfB) and
            ureohydrolase genes, complete cds.
ACCESSION   M34778
VERSION     M34778.1
KEYWORDS    DNA-binding protein; hmfB gene.
SOURCE      Methanothermus fervidus
  ORGANISM  Methanothermus fervidus
            Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
            Methanobacteriales; Methanothermaceae; Methanothermus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Sandman,K., Krzycki,J.A., Dobrinski,B., Lurz,R. and Reeve,J.N.
  TITLE     HMf, a DNA-binding protein isolated from the hyperthermophilic
            archaeon Methanothermus fervidus, is most closely related to
            histones
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87 (15), 5788-5791 (1990)
   PUBMED   2377617
REFERENCE   2  (bases 352 to 569)
  AUTHORS   Thomm,M., Sandman,K., Frey,G., Koller,G. and Reeve,J.N.
  TITLE     Transcription in vivo and in vitro of the histone-encoding gene
            hmfB from the hyperthermophilic archaeon Methanothermus fervidus
  JOURNAL   J. Bacteriol. 174 (11), 3508-3513 (1992)
   PUBMED   1592806
REFERENCE   3  (bases 1 to 1698)
  AUTHORS   Sandman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-1990) K. Sandman, Department of Microbiology,
            Ohio State University, Columbus, OH 43210, USA
COMMENT     On Aug 4, 1994 this sequence version replaced gi:149789.
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Methanothermus fervidus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:2180"
                     /map="1bp upstream of HindIII site"
     CDS             <1..299
                     /note="ORF; similar to threonine synthases"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="AAA72079.1"
                     /translation="ALRAIYESNXTALXVSDEEILKAQRLLAKKEGIGVEPASAASIA
                     GLIKLIDDGYLDHGERVVCVLTGHLLKDPKIAINACKKPLEVEPDFNLLKKIIG"
     gene            450..727
                     /gene="hmfB"
     regulatory      450..457
                     /regulatory_class="promoter"
                     /gene="hmfB"
     CDS             506..715
                     /gene="hmfB"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="AAA72080.1"
                     /translation="MELPIAPIGRIIKDAGAERVSDDARITLAKILEEMGRDIASEAI
                     KLARHAGRKTIKAEDIELAVRRFKK"
     regulatory      717..727
                     /regulatory_class="other"
                     /gene="hmfB"
                     /note="transcription termination signal"
     CDS             complement(733..1590)
                     /codon_start=1
                     /transl_table=11
                     /product="ureohydrolase"
                     /protein_id="AAA72081.1"
                     /translation="MGLKFAYSKDPSSLKYLSNKKVFGLLGVPFDSTSTYKPGSRFGP
                     LMIRQASYNFENYSLHYRKKLDVPIIDLGDIEVILGDFKNTCRNISEKVQEVLKKGMI
                     PIVLGGEHSITYGVVKTFDLSDVTILHFDAHMDMANTYAGKKFSHATVMRRIYELHPK
                     KIVQIGVRSCTKEEHEFVLNENIKYYTSRDIIEKFNMVLNEINKLDGPFYVTVDIDVL
                     DPGYAPGVGNPTPVGITPYHMEKFIEKIARKKIIGIDIVEVATDRIGDPAAMNAAKIL
                     YDFLFAIKI"
BASE COUNT          602 a          270 c          262 g          562 t
ORIGIN      
        1 aagctttgag agcaatatat gaatccaatn gaactgccct aancgtcagt gatgaagaaa
       61 tacttaaggc tcaaagacta ttagcaaaaa aagaaggtat aggtgttgag ccagcatcag
      121 ctgcaagtat tgcaggacta atcaaattaa tagatgatgg ctatttagat catggagaaa
      181 gagttgtatg cgtattgaca gggcatcttc taaaagatcc aaaaattgct ataaatgctt
      241 gtaaaaagcc tttagaagta gaaccagatt ttaatttact aaaaaagatt attggataaa
      301 tagaaaattt tattctataa aaaaacgcct ttgatacaat atctatatta tccatttata
      361 tggctatcaa agcacaatat aaacttttta tggttaataa gaatttcaca accaccatca
      421 aaataaaaca tcaacgatgc aaacaataaa tttatatagg ataaatttga taatattctt
      481 tcgtaagaat agagaggtgg taagtatgga attaccaata gcaccaattg gtagaattat
      541 aaaagatgca ggagccgaaa gagtcagtga tgatgcaaga ataactctag caaaaatttt
      601 agaagaaatg ggtagagaca ttgcttcaga agctataaaa ctagcaagac acgccggaag
      661 gaaaacaata aaagcagagg acattgaact agcagttcga agatttaaga aataatattt
      721 ttctttttcc ttttatattt ttattgcaaa taaaaaatca tacaaaattt ttgctgcatt
      781 catcgctgcg gggtcgccta ttctatctgt tgcaacttca acaatgtcaa tgccaataat
      841 tttttttcta gctatttttt caatgaattt ttccatatgg tagggagtga tacctacagg
      901 cgttggattt ccaacacctg gagcataacc aggatctaat acatctatat ccactgttac
      961 atagaatggt ccatctaatt tatttatttc atttaaaacc atgttaaatt tttcaattat
     1021 gtctcttgat gtatagtatt tgatattttc attaagaaca aattcatgtt cttcttttgt
     1081 acaagatctt acaccaattt ggacaatttt cttcggatgt aattcatata ttcgtcgcat
     1141 gactgttgca tgcgaaaact ttttaccagc ataagtattt gccatatcca tatgtgcatc
     1201 aaagtgtaga atagttacat cacttagatc aaatgttttc acaacaccat atgttatgct
     1261 atgttctcca ccaaggacaa taggaatcat tccttttttt aaaacttctt gaactttttc
     1321 agaaatatta cgacaagtat ttttaaagtc acctagaatt acctcaatat cacctaaatc
     1381 aatgatagga acatctagtt tttttctata gtgtaaacta tagttttcaa aattgtagga
     1441 tgcttgacga atcatcaatg gtccaaatct tgaccctggc ttatatgtgg atgtgctatc
     1501 aaaaggtact cctaacaatc caaaaacttt tttattagaa aggtatttaa gggaggaagg
     1561 gtccttagaa tatgcaaatt ttagacccat attatttaac tcttaaaagt tttgttttac
     1621 ccattgcttc taaatattca acttctgcac cttcagttaa tttatctttt aactcctcag
     1681 gtataggaac ttcaaatg
//