LOCUS ECOCYS 5755 bp DNA linear BCT 26-APR-1993 DEFINITION E.coli thiosulfate binding protein (cysP), sulfate permease (cysT, cysW, cysA) and o-acetylserine (thiol)-lyase-B (cysM) genes, complete cds. ACCESSION M32101 M38050 VERSION M32101.1 KEYWORDS cysA gene; cysM gene; cysP gene; cysR gene; cysT gene; cysW gene; o-acetylserine (thiol)-lyase-B; sulfate permease; thiosulfate binding protein. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 5755) AUTHORS Sirko,A., Hryniewicz,M., Hulanicka,D. and Bock,A. TITLE Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster JOURNAL J. Bacteriol. 172 (6), 3351-3357 (1990) PUBMED 2188958 REFERENCE 2 (bases 1 to 5755) AUTHORS Hryniewicz,M., Sirko,A., Palucha,A., Bock,A. and Hulanicka,D. TITLE Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding JOURNAL J. Bacteriol. 172 (6), 3358-3366 (1990) PUBMED 2188959 COMMENT Original source text: E.coli K12 DNA. Draft entry and computer-readable sequence [1] kindly submitted by D.Halanicka, 14-FEB-1990. FEATURES Location/Qualifiers source 1..5755 /organism="Escherichia coli" /mol_type="genomic DNA" /db_xref="taxon:562" regulatory 516..521 /regulatory_class="minus_10_signal" gene 559..1575 /gene="cysP" CDS 559..1575 /gene="cysP" /codon_start=1 /transl_table=11 /product="thiosulfate binding protein" /protein_id="AAA23636.1" /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA AGRN" gene 1575..2408 /gene="cysT" CDS 1575..2408 /gene="cysT" /codon_start=1 /transl_table=11 /protein_id="AAA23637.1" /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG H" gene 2408..3283 /gene="cysW" CDS 2408..3283 /gene="cysW" /note="sulfate permease (cysW)" /codon_start=1 /transl_table=11 /product="sulfate permease" /protein_id="AAA23638.1" /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL ENQEKRAQQEEHHEH" gene 3273..4370 /gene="cysA" CDS 3273..4370 /gene="cysA" /codon_start=1 /transl_table=11 /protein_id="AAA23639.1" /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAHVSGGQKQRVALARALAVEPQILLL DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE RLFVGLQHARLYNGDERIETRDEELALAQSA" gene 4505..5416 /gene="cysM" CDS 4505..5416 /gene="cysM" /EC_number="2.5.1.47" /codon_start=1 /transl_table=11 /product="o-acetylserine (thiol)-lyase-B" /protein_id="AAA23640.1" /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVV AIICDRGDRYLSTGVFGEEHFSQGAGI" misc_feature 5737..5755 /note="right end of mu" BASE COUNT 1259 a 1477 c 1682 g 1337 t ORIGIN 52 min on K12 map. 1 gttaacgcca tttgcccggg atacgtgcgc acaccaatgg cggaaagcat tgcccgccag 61 tcgaacccgg aagatccaga gtcggtgctg actgaaatgg cgaaagcaat cccgatgcgt 121 cgcctcgccg atccgctgga agtcggcgaa ctggcggcct tcctcgcatc ggatgaatcc 181 agctatttaa ccggtacaca gaatgtgatt gatggcggca gcacactgcc ggagacggtt 241 agcgtcggta tctgattcac ctctgtttcc tccctgcatt tgtggggagg atttcgtctt 301 gaactaagtt caccaggcta ttttatttgt cattttggcc ccgggcagtg ctcgaaatcc 361 tcacgtacta tgtgtacgct ccggtttctc cgcgctgttc gtgtccaaac tgactgcaac 421 aattacgcct gttgaaccaa gttcttattc ccttttcaac ttccaaatca ccaaacggta 481 tataaaaccg ttactccttt cacgtccgtt ataaatatga tggctattag aaagtcatta 541 aatttataag ggtgcgcaat ggccgttaac ttactgaaaa agaactcact cgcgctggtc 601 gcttctctgc tgctggcggg ccatgtacag gcaacggaac tgctgaacag ttcttatgac 661 gtctcccgcg agctgtttgc cgccctgaat ccgccgtttg agcaacaatg ggcaaaagat 721 aacggcggcg acaaactgac gataaaacaa tctcatgccg ggtcatcaaa acaggcgctg 781 gcgattttac agggcttaaa agccgacgtt gtcacttata accaggtgac cgacgtacaa 841 atcctgcacg ataaaggcaa gctgatcccg gccgactggc agtcgcgcct gccgaataat 901 agctcgccgt tctactccac catgggcttc ctggtgcgta agggtaaccc gaagaatatc 961 cacgattgga acgacctggt gcgctccgac gtgaagctga ttttcccgaa cccgaaaacg 1021 tcgggtaacg cgcgttatac ctatctggcg gcatggggcg cagcggataa agctgacggt 1081 ggtgacaaag gcaaaaccga acagtttatg acccagttcc tgaaaaacgt tgaagtgttc 1141 gatactggcg gtcgtggcgc gaccaccact tttgccgagc gcggcctggg cgatgtgctg 1201 attagcttcg aatcggaagt gaacaacatc cgtaaacagt atgaagcgca gggctttgaa 1261 gtggtgattc cgaaaaccaa cattctggcg gaattcccgg tggcgtgggt tgataaaaac 1321 gtgcaggcca acggtacgga aaaagccgcc aaagcctatc tgaactggct ctatagcccg 1381 caggcgcaaa ccatcatcac cgactattac taccgcgtga ataacccgga ggtgatggac 1441 aaactgaaag acaaattccc gcagaccgag ctgttccgcg tggaagacaa atttggctcc 1501 tggccggaag tgatgaaaac ccacttcacc agcggcggcg agttagacaa gctgttagcg 1561 gcggggcgta actgatgttt gctgtctcct ccagacgcgt gctgccgggc tttaccttaa 1621 gcctcggcac cagtctgctg tttgtgtgcc tgattttgct gctgccgctc tccgcgctgg 1681 tgatgcaact ggcccagatg agctgggcgc agtactggga ggtgatcacc aacccgcagg 1741 tggtcgcggc ctacaaagta acgctgctgt cggcgtttgt ggcatcgatt tttaacggcg 1801 ttttcggtct gctgatggcg tggatcctaa cccgctatcg cttcccaggc cgcacgctgc 1861 ttgatgcgct gatggattta ccctttgcgc tgccaacggc tgtcgccggt ttaacgctgg 1921 cctcgctctt ttccgtaaac ggtttttacg gtgaatggct ggcgaagttt gatatcaaag 1981 tcacctatac atggctgggg attgcggtgg ctatggcctt taccagcatt ccgtttgtgg 2041 tgcgtaccgt gcagccggtg ctggaagagt taggcccgga atatgaagaa gcggcggaaa 2101 cgcttggtgc aacgcgctgg cagagtttct gcaaagtggt gctgccggag ctttctccgg 2161 cgctggtggc gggcgtggcg ctgtcgttta cccgtagtct tggtgaattt ggcgcggtga 2221 tttttatcgc cggaaatatc gcgtggaaga cggaagtgac gtcgctgatg atttttgtgc 2281 gcttacagga gtttgattac ccggcagcga gcgcgattgc ttcggtgatc ctcgcggcat 2341 ctctgctgct gctgttctca attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag 2401 gtcattaatg gcggaagtta cccaattgaa gcgttatgac gcgcgcccga ttaactgggg 2461 caaatggttt ctgattggca tcgggatgct ggtttcggcg ttcatcctgc tggtgccgat 2521 gatttacatc ttcgtgcagg cattcagcaa ggggctgatg ccggttttac agaatctggc 2581 cgatccggac atgctgcacg ccatctggct gacggtgatg atcgcgctga ttgccgtacc 2641 ggtaaacctg gtgttcggca ttctgctggc ctggctggtg acgcgcttta acttccctgg 2701 acgccagtta ctgctgacgc tactggacat tccgtttgcc gtatcgccgg tggttgccgg 2761 tctggtgtat ttgctgttct acggctctaa cggcccgctc ggcggttggc tcgacgagca 2821 taacctgcaa attatgttct cctggccggg aatggtgctg gtcaccatct tcgtgacgtg 2881 tccgtttgtg gtgcgcgaac tggtgccggt gatgttaagc cagggcagcc aggaagacga 2941 agcggcgatt ttgcttggcg cgtccggctg gcagatgttc cgtcgcgtca cattaccgaa 3001 catccgctgg gcgctgcttt atggcgtggt gttgaccaac gcccgcgcaa ttggcgagtt 3061 tggcgcggtg tcggtggttt ccggctcgat tcgcggcgaa accctgtcgc tgccgttaca 3121 gattgaattg ctggagcagg actacaacac cgtcggctcc tttaccgctg cggcgctgtt 3181 aacgctgatg gcgattatca ccctgttttt aaaaagtatg ttgcagtggc gcctggagaa 3241 tcaggaaaaa cgcgcacagc aggaggaaca tcatgagcat tgagattgcc aatattaaga 3301 agtcgtttgg tcgcacccag gtgctgaacg atatctcact ggatattcct tcaggtcaga 3361 tggtcgcgtt gctggggccg tccggttccg ggaaaaccac gctgctgcgc attatcgccg 3421 ggctggagca tcaaaccagc gggcatattc gcttccacgg caccgacgtg agccgcctgc 3481 acgcacgtga tcgtaaagtc ggtttcgtgt tccagcatta cgcgctgttc cgccatatga 3541 cggtgttcga caatatcgct tttggcctga cggtgctgcc gcgtcgcgag cgcccgaatg 3601 ccgcagccat caaagcgaaa gtgacaaaat tgctggaaat ggtccagctt gcccatctgg 3661 cggatcgtta tccggcgcac gtttccggcg gccagaaaca gcgcgtggcg ctggcgcgcg 3721 cgctggctgt ggaaccgcaa attctgctgc ttgatgaacc gtttggcgcg ctggatgcgc 3781 aggtgcgtaa agagctgcgt cgctggctgc gtcaactcca tgaagaacta aaattcacca 3841 gcgtttttgt gacccacgat caggaagaag cgaccgaagt agctgatcgt gtagttgtga 3901 tgagccaggg caatattgaa caggctgacg cgccggatca ggtatggcgc gaaccggcga 3961 cccgttttgt gctcgaattt atgggcgaag tgaaccgcct gcagggaacc attcgcggcg 4021 ggcagttcca tgttggcgcg catcgctggc cgctgggcta cacacctgcg tatcaggggc 4081 cggtggatct cttcctgcgc ccttgggaag tggatatcag ccgccgtacc agcctcgatt 4141 cgccgctgcc ggtacaggta ctggaagcca gcccgaaagg tcactacacc caattagtgg 4201 tgcagccgct ggggtggtac aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc 4261 cgcagcgtgg cgagcgttta ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg 4321 agcgtatcga aacccgcgat gaggaacttg ctctcgcaca aagcgcctga taggttgagt 4381 gaatgttaaa cgcccggagg cgcttcccgc gagtccgggc tttttaatgg caaggtttgt 4441 aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg caacaccacg tatggataga 4501 gatcgtgagt acattagaac aaacaatagg caatacgcct ctggtgaagt tgcagcgaat 4561 ggggccggat aacggcagtg aagtgtggtt aaaactggaa ggcaataacc cggcaggttc 4621 ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg gaaaagcgcg gggaaattaa 4681 accgggtgat gtcttaatcg aagccaccag tggtaacacc ggcattgcgc tggcaatgat 4741 tgccgcgctg aaaggctatc gcatgaaatt gctgatgccc gacaacatga gccaggaacg 4801 ccgtgcggcg atgcgtgctt atggtgcgga actgattctt gtcaccaaag agcagggcat 4861 ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt ggcgaaggaa agctgctcga 4921 tcagttcaat aatcccgata acccttatgc gcattacacc accactgggc cggaaatctg 4981 gcagcaaacc ggcgggcgca tcactcattt tgtctccagc atggggacga ccggcactat 5041 caccggcgtc tcacgcttta tgcgcgaaca atccaaaccg gtgaccattg tcggcctgca 5101 accggaagag ggcagcagca ttcccggcat tcgccgctgg cctacggaat atctgccggg 5161 gattttcaac gcttctctgg tggatgaggt gctggatatt catcagcgcg atgcggaaaa 5221 caccatgcgc gaactggcgg tgcgggaagg aatattctgt ggcgtcagct ccggcggcgc 5281 ggttgccgga gcactgcggg tggcaaaagc taaccctgac gcggtggtgg tggcgatcat 5341 ctgcgatcgt ggcgatcgct acctttctac cggggtgttt ggggaagagc attttagcca 5401 gggggcgggg atttaaggat taatagcatc ggagactgat gacaaacgca aaactgcctg 5461 atgcgctacg cttatcaggc ctacaaggtt tctgcaatat attgaattag cacgattttg 5521 taggccggat aaggcgttta cgccgcatcc ggcataaaca aagcgcactt ttttaacagt 5581 tgttgctgcc gacaaatgca gtatttaatt ttcgtgagga aacgccgtaa ggtcattgaa 5641 gcggcgcacg aaaaaccgaa agcgtttcac gataaatgcg aaaactttac gtttcgcgct 5701 tcaaatgaaa cagatgtatt aattactact ttttattcat tacatgggga tccag //