LOCUS       ECOHYDHGA               1740 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  Escherichia coli hydHG operon regulating labile hydrogenase
            activity.
ACCESSION   M28369
VERSION     M28369.1
KEYWORDS    hydrogenase.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Stoker,K., Reijnders,W.N., Oltmann,L.F. and Stouthamer,A.H.
  TITLE     Initial cloning and sequencing of hydHG, an operon homologous to
            ntrBC and regulating the labile hydrogenase activity in Escherichia
            coli K-12
  JOURNAL   J. Bacteriol. 171 (8), 4448-4456 (1989)
   PUBMED   2666400
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     gene            1..415
                     /gene="hydH"
     CDS             <1..415
                     /gene="hydH"
                     /codon_start=2
                     /transl_table=11
                     /protein_id="AAA24003.1"
                     /translation="VSQDANSREIQLRFTANDTLPEIQADPDRLTQVVLNLYLNAIQA
                     IGQHGVISVTATKAGGVKISVTDSGKGIAADQLDAIFTPYFTTKAEGTGLGLAVVHNI
                     VEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG"
     gene            412..1725
                     /gene="hydG"
     CDS             412..1725
                     /gene="hydG"
                     /note="hydG protein"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24004.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATWKKRSHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPC
                     EATVLIHGDSARKELVARGLHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTG
                     ADKRREGPFVEADGGTCLDEIGDISPMMQVRLLRAIQEREVQRVGSNQIISVDVRLIA
                     ATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRRG
                     KRFYAPGLDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLGIASTPIPLGQS
                     QDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
BASE COUNT          408 a          431 c          534 g          367 t
ORIGIN      
        1 ggtaagtcag gatgcaaaca gccgggagat ccagttacgc tttaccgcca acgacacatt
       61 accggaaatt caggccgacc cggacaggct gactcaggtc gtgttgaatc tctatctcaa
      121 tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccacga aagccggcgg
      181 ggtaaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc ttgatgccat
      241 cttcactccg tacttcacca ctaaagccga aggcaccgga ttggggctgg cggtcgtgca
      301 taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg gaaaaggctc
      361 aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag gatgacgcac
      421 gataatatcg atattctggt ggtggatgat gacattagcc actgcactat tttgcaggct
      481 ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca ggcgttggag
      541 caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc ggagatggac
      601 ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt gctgattatg
      661 actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc gctggattat
      721 ctcatcaagc cgctggattt cgataaccta caggcgacgt ggaaaaagcg ctcgcatacg
      781 cacagtattg atgctgaaac acctgcggtg actgccagcc agttcggtat ggtcggtaaa
      841 agcccggcga tgcaacacct gctcagtgaa atcgccctcg tcgcgccatg cgaagccacg
      901 gtactgatcc acggcgattc ggcacgtaaa gagctggtcg ccaggggact tcacgccagt
      961 agcgcacgta gcgaaaaacc gctggtaacg ctcaactgtg cggcactcaa cgaatccttg
     1021 ctggaatctg aattgttcgg tcacgaaaaa ggggcgttta ctggagccga taaacgccgg
     1081 gaagggccct ttgttgaggc ggacggcggc acgtgtctcg atgaaattgg cgatatctcg
     1141 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
     1201 agcaaccaga ttatctcggt tgatgtccgg ctgattgcgg cgacccatcg cgatcttgcc
     1261 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
     1321 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tggccatttt
     1381 ctgcagcgct ttgccgagcg taatcgaagg ggtaaaaggt tttacgcccc aggactggat
     1441 ctgttgattc attacgactg gccgggaaat attcgtgagc tggaaaacgc ggtggaacgg
     1501 gcagtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct gggcattgcc
     1561 agtacgccga tcccgctggg acaaagtcag gatattcagc cgttggtgga agtggaaaaa
     1621 gaggtgattc tggcggcgct ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
     1681 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
//