LOCUS       YSCGLUI                 2538 bp    DNA     linear   PLN 27-APR-1993
DEFINITION  Yeast (S.fibuligera) glucoamylase (GLUI) gene, complete cds.
ACCESSION   M17355
VERSION     M17355.1
KEYWORDS    glucoamylase.
SOURCE      Saccharomycopsis fibuligera
  ORGANISM  Saccharomycopsis fibuligera
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycopsidaceae; Saccharomycopsis.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Itoh,T., Ohtsuki,I., Yamashita,I. and Fukui,S.
  TITLE     Nucleotide sequence of the glucoamylase gene GLU1 in the yeast
            Saccharomycopsis fibuligera
  JOURNAL   J. Bacteriol. 169 (9), 4171-4176 (1987)
   PUBMED   3114236
REFERENCE   2  (sites)
  AUTHORS   Itoh,T., Sakata,Y., Akada,R., Mini,O. and Yamashita,I.
  TITLE     Construction and characterization of mutant glucoamylases from the
            yeast Saccharomycopsis fibuligera
  JOURNAL   Agric. Biol. Chem. 53, 3159-3167 (1991)
COMMENT     Original source text: Yeast (S.fibuligera, strain HUT7212) DNA.
            Draft entry and printed copy for [1] kindly provided by
            I.Yamashita, 19-JAN-1988.
            A poly-adenylation site is located at positions 2061-2066 and a
            Hogness box at 178-185.
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Saccharomycopsis fibuligera"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4944"
     CDS             322..1881
                     /note="glucoamylase precursor"
                     /codon_start=1
                     /protein_id="AAA34649.1"
                     /translation="MKFGVLFSVFAAIVSALPLQEGPLNKRAYPSFEAYSNYKVDRTD
                     LETFLDKQKEVSLYYLLQNIAYPEGQFNNGVPGTVIASPSTSNPDYYYQWTRDSAITF
                     LTVLSELEDNNFNTTLAKAVEYYINTSYNLQRTSNPSGSFDDENHKGLGEPKFNTDGS
                     AYTGAWGRPQNDGPALRAYAISRYLNDVNSLNEGKLVLTDSGDINFSSTEDIYKNIIK
                     PDLEYVIGYWDSTGFDLWEENQGRHFFTSLVQQKALAYAVDIAKSFDDGDFANTLSST
                     ASTLESYLSGSDGGFVNTDVNHIVENPDLLQQNSRQGLDSATYIGPLLTHDIGESSST
                     PFDVDNEYVLQSYYLLLEDNKDRYSVNSAYSAGAAIGRYPEDVYNGDGSSEGNPWFLA
                     TAYAAQVPYKLAYDAKSASNDITINKINYDFFNKYIVDLSTINSAYQSSDSVTIKSGS
                     DEFNTVADNLVTFGDSFLQVILDHINDDGSLNEQLNRYTGYSTGAYSLTWSSGALLEA
                     IRLRNKVKALA"
     sig_peptide     322..402
                     /note="glucoamylase signal peptide"
     mat_peptide     403..1878
                     /product="glucoamylase"
BASE COUNT          766 a          462 c          450 g          860 t
ORIGIN      5 bp upstream of PstI site.
        1 ctgcagtcaa catgcgcatt cgaaggataa aagatgttcc gaggggttaa actgattatg
       61 actacacatc gtagatcaca acattatgcc atagcacttt tttggaagtg ttcaaaaaat
      121 ataagcgtgt cttcaatata gcggcgaggt tctcttggtt tggtaaatag aaattggtat
      181 aaatacttac tacatcacct cattttctac agttttacac aatataatct ttatatatca
      241 aaaatattca aacaaaatca ataaaaacag aactttcttt tttcaaattt ttttgctacc
      301 ttcctgaaac ttctatttgc tatgaaattc ggtgttttat tttccgtctt tgctgctatt
      361 gttagtgctt tacctttgca agaaggtcct ttgaacaaaa gagcctatcc ttcttttgaa
      421 gcttattcaa actataaagt tgacagaact gacttggaaa ccttcttgga caaacaaaaa
      481 gaagtatctt tatactatct tttacaaaac attgcttatc ctgaaggcca atttaataat
      541 ggtgttcctg gtactgttat tgcttctcca tcaacctcta atccggacta ctattaccaa
      601 tggaccagag attccgcaat tacatttttg acagttcttt ctgaactaga agataataac
      661 ttcaatacca ctttggccaa ggcagttgag tactacatta acaccagtta caaccttcaa
      721 agaaccagta acccaagtgg cagctttgat gatgaaaatc ataaaggctt gggagaacca
      781 aaatttaaca cagatggttc tgcatacacc ggagcttggg ggagaccgca aaatgatggt
      841 cctgctttga gagcttatgc tatcagtaga tacttgaatg atgtcaattc tttaaatgaa
      901 ggtaaattag tattgactga ttcaggtgat atcaactttt cttcaactga agatatttac
      961 aaaaatatca tcaaaccaga cttggaatat gttatagggt actgggattc tactgggttt
     1021 gatctttggg aggaaaacca aggcagacac ttttttacaa gcttggttca acagaaagcc
     1081 cttgcttatg ctgtcgatat tgccaaaagt tttgacgacg gcgactttgc gaacacactt
     1141 tcttcgactg cttctaccct cgaaagttat ttgagtggca gtgatggtgg atttgttaat
     1201 actgatgtta accacattgt tgaaaaccca gatttgcttc aacaaaactc tagacaaggt
     1261 ctagattcag ccacatatat tggcccactt ttgactcatg atattggtga aagcagctca
     1321 actccatttg atgttgacaa tgagtatgtt ttgcaatcat attacttgtt attggaggat
     1381 aacaaagaca gatactctgt taacagtgct tattctgctg gtgcagctat tggcagatac
     1441 ccagaagatg tttacaatgg tgatggttca tctgaaggca atccatggtt cttagctact
     1501 gcctatgctg cccaagttcc atacaaactt gcttatgatg caaagtcggc ctcaaatgac
     1561 attaccatta acaagattaa ctacgatttt tttaacaagt atattgttga tttatctacc
     1621 atcaattctg cttaccagtc ttctgatagt gtcaccatta aaagtggctc tgatgaattt
     1681 aacacggttg ctgataattt ggtcacattc ggtgattcct ttttgcaagt cattttggat
     1741 catattaatg atgatggctc cttgaatgaa caacttaaca gatataccgg ttattccacc
     1801 ggtgcctact ctttgacatg gagcagtggt gctcttcttg aagctattag acttagaaat
     1861 aaggtcaagg ctttggctta agtgctttga aaagtaagtg tttttccttt gtggagaaca
     1921 tgcaaccata atccgacagc tttgttatga tactactttt ttgatatttt ccccttcttt
     1981 gaccaggcta tctaattatg gctctatttt ctttctactt tttggctcga gcttctgaaa
     2041 catatacgtc agcgagtatc aataaacttt tatttgttac ttttttttag gatatcttta
     2101 actggtaact tttggagatt atcaatagag ttaatagtat cgtggcgcct gcatatatag
     2161 ttaatgctat tatttggaat ttacactgtc agcacgtttc cagtcttggt agtcaggaat
     2221 agacttatga aataacagaa agggcatccg tgaccattta tcatcaaaat tatatcaaaa
     2281 caattggtcg tggttcctac aaatattgga aaaaatattc tctgagatgt attttcctcc
     2341 gattgggtcg tttctcttat actattcctg aatctaccta acactactag tagactgatg
     2401 tgtctatcta tcgtttaaat gagactaaga tggaaaagat tttgttgcaa agaaacaatt
     2461 tttttcaata ttgtaaagtg aaaacattca atacttttga atttaatttc caagaataca
     2521 aaatcggtgt tgctgcag
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